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Bernd NIDETZKY
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Articles

The oxyanion hole of Pseudomonas fluorescens mannitol 2-dehydrogenase: a novel structural motif for electrostatic stabilization in alcohol dehydrogenase active sites

Mario Klimacek, Bernd Nidetzky
Journal: Biochemical Journal
Biochem J (2010) 425 (2): 455–463.
https://doi.org/10.1042/BJ20091441
Published: 23 December 2009
View article titled, The oxyanion hole of Pseudomonas fluorescens mannitol 2-dehydrogenase: a novel structural motif for electrostatic stabilization in alcohol dehydrogenase active sites
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Includes: Supplementary data
Articles

The catalytic mechanism of NADH-dependent reduction of 9,10-phenanthrenequinone by Candida tenuis xylose reductase reveals plasticity in an aldo-keto reductase active site

Simone L. Pival, Mario Klimacek, Bernd Nidetzky
Journal: Biochemical Journal
Biochem J (2009) 421 (1): 43–49.
https://doi.org/10.1042/BJ20090128
Published: 12 June 2009
View article titled, The catalytic mechanism of NADH-dependent reduction of 9,10-phenanthrenequinone by Candida tenuis xylose reductase reveals plasticity in an aldo-keto reductase active site
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Includes: Supplementary data
Articles

Biochemical characterization and mutational analysis of the mononuclear non-haem Fe 2+ site in Dke1, a cupin-type dioxygenase from Acinetobacter johnsonii

Stefan Leitgeb, Grit D. Straganz, Bernd Nidetzky
Journal: Biochemical Journal
Biochem J (2009) 418 (2): 403–411.
https://doi.org/10.1042/BJ20081161
Published: 11 February 2009
View article titled, Biochemical characterization and mutational analysis of the mononuclear non-haem Fe 2+ site in Dke1, a cupin-type dioxygenase from Acinetobacter johnsonii
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Includes: Supplementary data
Articles

Catalytic mechanism of Zn 2+ -dependent polyol dehydrogenases: kinetic comparison of sheep liver sorbitol dehydrogenase with wild-type and Glu 154 →Cys forms of yeast xylitol dehydrogenase

Mario Klimacek, Heidemarie Hellmer, Bernd Nidetzky
Journal: Biochemical Journal
Biochem J (2007) 404 (3): 421–429.
https://doi.org/10.1042/BJ20061384
Published: 29 May 2007
View article titled, Catalytic mechanism of Zn 2+ -dependent polyol dehydrogenases: kinetic comparison of sheep liver sorbitol dehydrogenase with wild-type and Glu 154 →Cys forms of yeast xylitol dehydrogenase
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Includes: Supplementary data
Articles

Acid–base catalysis in Leuconostoc mesenteroides sucrose phosphorylase probed by site-directed mutagenesis and detailed kinetic comparison of wild-type and Glu 237 →Gln mutant enzymes

Alexandra Schwarz, Lothar Brecker, Bernd Nidetzky
Journal: Biochemical Journal
Biochem J (2007) 403 (3): 441–449.
https://doi.org/10.1042/BJ20070042
Published: 12 April 2007
View article titled, Acid–base catalysis in Leuconostoc mesenteroides sucrose phosphorylase probed by site-directed mutagenesis and detailed kinetic comparison of wild-type and Glu 237 →Gln mutant enzymes
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Articles

Structure–function relationships for Schizophyllum commune trehalose phosphorylase and their implications for the catalytic mechanism of family GT-4 glycosyltransferases

Christiane Goedl, Richard Griessler, Alexandra Schwarz, Bernd Nidetzky
Journal: Biochemical Journal
Biochem J (2006) 397 (3): 491–500.
https://doi.org/10.1042/BJ20060029
Published: 13 July 2006
View article titled, Structure–function relationships for Schizophyllum commune trehalose phosphorylase and their implications for the catalytic mechanism of family GT-4 glycosyltransferases
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Includes: Supplementary data
Articles

Probing the substrate binding site of Candida tenuis xylose reductase (AKR2B5) with site-directed mutagenesis

Regina Kratzer, Stefan Leitgeb, David K. Wilson, Bernd Nidetzky
Journal: Biochemical Journal
Biochem J (2006) 393 (1): 51–58.
https://doi.org/10.1042/BJ20050831
Published: 12 December 2005
View article titled, Probing the substrate binding site of Candida tenuis xylose reductase (AKR2B5) with site-directed mutagenesis
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Articles

Electrostatic stabilization in a pre-organized polar active site: the catalytic role of Lys-80 in Candida tenuis xylose reductase (AKR2B5) probed by site-directed mutagenesis and functional complementation studies

Regina Kratzer, Bernd Nidetzky
Journal: Biochemical Journal
Biochem J (2005) 389 (2): 507–515.
https://doi.org/10.1042/BJ20050167
Published: 05 July 2005
View article titled, Electrostatic stabilization in a pre-organized polar active site: the catalytic role of Lys-80 in Candida tenuis xylose reductase (AKR2B5) probed by site-directed mutagenesis and functional complementation studies
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Articles

Catalytic mechanism of α-retaining glucosyl transfer by Corynebacterium callunae starch phosphorylase: the role of histidine-334 examined through kinetic characterization of site-directed mutants

Alexandra SCHWARZ, Francesco Maria PIERFEDERICI, Bernd NIDETZKY
Journal: Biochemical Journal
Biochem J (2005) 387 (2): 437–445.
https://doi.org/10.1042/BJ20041593
Published: 05 April 2005
View article titled, Catalytic mechanism of α-retaining glucosyl transfer by Corynebacterium callunae starch phosphorylase: the role of histidine-334 examined through kinetic characterization of site-directed mutants
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Articles

The coenzyme specificity of Candida tenuis xylose reductase (AKR2B5) explored by site-directed mutagenesis and X-ray crystallography

Barbara PETSCHACHER, Stefan LEITGEB, Kathryn L. KAVANAGH, David K. WILSON, Bernd NIDETZKY
Journal: Biochemical Journal
Biochem J (2005) 385 (1): 75–83.
https://doi.org/10.1042/BJ20040363
Published: 14 December 2004
View article titled, The coenzyme specificity of Candida tenuis xylose reductase (AKR2B5) explored by site-directed mutagenesis and X-ray crystallography
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Articles

On the role of Brønsted catalysis in Pseudomonas fluorescens mannitol 2-dehydrogenase

Mario KLIMACEK, Kathryn L. KAVANAGH, David K. WILSON, Bernd NIDETZKY
Journal: Biochemical Journal
Biochem J (2003) 375 (1): 141–149.
https://doi.org/10.1042/bj20030733
Published: 01 October 2003
View article titled, On the role of Brønsted catalysis in Pseudomonas fluorescens mannitol 2-dehydrogenase
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Articles

Structure of xylose reductase bound to NAD+ and the basis for single and dual co-substrate specificity in family 2 aldo-keto reductases

Kathryn L. KAVANAGH, Mario KLIMACEK, Bernd NIDETZKY, David K. WILSON
Journal: Biochemical Journal
Biochem J (2003) 373 (2): 319–326.
https://doi.org/10.1042/bj20030286
Published: 15 July 2003
View article titled, Structure of xylose reductase bound to NAD+ and the basis for single and dual co-substrate specificity in family 2 aldo-keto reductases
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Articles

A catalytic consensus motif for d-mannitol 2-dehydrogenase, a member of a polyol-specific long-chain dehydrogenase family, revealed by kinetic characterization of site-directed mutants of the enzyme from Pseudomonas fluorescens

Mario KLIMACEK, Bernd NIDETZKY
Journal: Biochemical Journal
Biochem J (2002) 367 (1): 13–18.
https://doi.org/10.1042/bj20020932
Published: 01 October 2002
View article titled, A catalytic consensus motif for d-mannitol 2-dehydrogenase, a member of a polyol-specific long-chain dehydrogenase family, revealed by kinetic characterization of site-directed mutants of the enzyme from Pseudomonas fluorescens
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Articles

Catalytic reaction profile for NADH-dependent reduction of aromatic aldehydes by xylose reductase from Candida tenuis

Peter MAYR, Bernd NIDETZKY
Journal: Biochemical Journal
Biochem J (2002) 366 (3): 889–899.
https://doi.org/10.1042/bj20020080
Published: 15 September 2002
View article titled, Catalytic reaction profile for NADH-dependent reduction of aromatic aldehydes by xylose reductase from Candida tenuis
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Articles

Substrate-binding recognition and specificity of trehalose phosphorylase from Schizophyllum commune examined in steady-state kinetic studies with deoxy and deoxyfluoro substrate analogues and inhibitors

Christian EIS, Bernd NIDETZKY
Journal: Biochemical Journal
Biochem J (2002) 363 (2): 335–340.
https://doi.org/10.1042/bj3630335
Published: 08 April 2002
View article titled, Substrate-binding recognition and specificity of trehalose phosphorylase from Schizophyllum commune examined in steady-state kinetic studies with deoxy and deoxyfluoro substrate analogues and inhibitors
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Articles

α-Retaining glucosyl transfer catalysed by trehalose phosphorylase from Schizophyllum commune: mechanistic evidence obtained from steady-state kinetic studies with substrate analogues and inhibitors

Bernd NIDETZKY, Christian EIS
Journal: Biochemical Journal
Biochem J (2001) 360 (3): 727–736.
https://doi.org/10.1042/bj3600727
Published: 10 December 2001
View article titled, α-Retaining glucosyl transfer catalysed by trehalose phosphorylase from Schizophyllum commune: mechanistic evidence obtained from steady-state kinetic studies with substrate analogues and inhibitors
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Articles

Fungal trehalose phosphorylase: kinetic mechanism, pH-dependence of the reaction and some structural properties of the enzyme from Schizophyllum commune

Christian EIS, Mark WATKINS, Thomas PROHASKA, Bernd NIDETZKY
Journal: Biochemical Journal
Biochem J (2001) 356 (3): 757–767.
https://doi.org/10.1042/bj3560757
Published: 08 June 2001
View article titled, Fungal trehalose phosphorylase: kinetic mechanism, pH-dependence of the reaction and some structural properties of the enzyme from Schizophyllum commune
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Articles

Role of non-covalent enzyme–substrate interactions in the reaction catalysed by cellobiose phosphorylase from Cellulomonas uda

Bernd NIDETZKY, Christian EIS, Martin ALBERT
Journal: Biochemical Journal
Biochem J (2000) 351 (3): 649–659.
https://doi.org/10.1042/bj3510649
Published: 24 October 2000
View article titled, Role of non-covalent enzyme–substrate interactions in the reaction catalysed by cellobiose phosphorylase from Cellulomonas uda
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Articles

Mechanism of thermal denaturation of maltodextrin phosphorylase from Escherichia coli

Richard GRIEßLER, Sabato D'AURIA, Reinhard SCHINZEL, Fabio TANFANI, Bernd NIDETZKY
Journal: Biochemical Journal
Biochem J (2000) 346 (2): 255–263.
https://doi.org/10.1042/bj3460255
Published: 22 February 2000
View article titled, Mechanism of thermal denaturation of maltodextrin phosphorylase from Escherichia coli
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Articles

Binding energy and specificity in the catalytic mechanism of yeast aldose reductases

Bernd NIDETZKY, Peter MAYR, Philipp HADWIGER, Arnold E. STüTZ
Journal: Biochemical Journal
Biochem J (1999) 344 (1): 101–107.
https://doi.org/10.1042/bj3440101
Published: 08 November 1999
View article titled, Binding energy and specificity in the catalytic mechanism of yeast aldose reductases
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Articles

Characterization of trehalose phosphorylase from Schizophyllum commune

Christian EIS, Bernd NIDETZKY
Journal: Biochemical Journal
Biochem J (1999) 341 (2): 385–393.
https://doi.org/10.1042/bj3410385
Published: 08 July 1999
View article titled, Characterization of trehalose phosphorylase from Schizophyllum commune
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Articles

Structural and functional properties of a yeast xylitol dehydrogenase, a Zn2+-containing metalloenzyme similar to medium-chain sorbitol dehydrogenases

Regina LUNZER, Yasmine MAMNUN, Dietmar HALTRICH, Klaus D. KULBE, Bernd NIDETZKY
Journal: Biochemical Journal
Biochem J (1998) 336 (1): 91–99.
https://doi.org/10.1042/bj3360091
Published: 15 November 1998
View article titled, Structural and functional properties of a yeast xylitol dehydrogenase, a Zn2+-containing metalloenzyme similar to medium-chain sorbitol dehydrogenases
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Articles

NAD(P)H-dependent aldose reductase from the xylose-assimilating yeast Candida tenuis : Isolation, characterization and biochemical properties of the enzyme

Wilfried NEUHAUSER, Dietmar HALTRICH, Klaus D. KULBE, Bernd NIDETZKY
Journal: Biochemical Journal
Biochem J (1997) 326 (3): 683–692.
https://doi.org/10.1042/bj3260683
Published: 15 September 1997
View article titled, NAD(P)H-dependent aldose reductase from the xylose-assimilating yeast Candida tenuis : Isolation, characterization and biochemical properties of the enzyme
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Articles

α -1,4-D-Glucan phosphorylase of gram-positive Corynebacterium callunae : isolation, biochemical properties and molecular shape of the enzyme from solution X-ray scattering

Andreas WEINHÄUSEL, Richard GRIESSLER, Angelika KREBS, Peter ZIPPER, Dietmar HALTRICH, Klaus D. KULBE, Bernd NIDETZKY
Journal: Biochemical Journal
Biochem J (1997) 326 (3): 773–783.
https://doi.org/10.1042/bj3260773
Published: 15 September 1997
View article titled, α -1,4-D-Glucan phosphorylase of gram-positive Corynebacterium callunae : isolation, biochemical properties and molecular shape of the enzyme from solution X-ray scattering
Open the PDF for α -1,4-D-Glucan phosphorylase of gram-positive Corynebacterium callunae : isolation, biochemical properties and molecular shape of the enzyme from solution X-ray scattering in another window