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E W Thomas
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Articles

Structure of chymopapain M the late-eluted chymopapain deduced by comparative modelling techniques and active-centre characteristics determined by pH-dependent kinetics of catalysis and reactions with time-dependent inhibitors: the Cys-25/His-159 ion-pair is insufficient for catalytic competence in both chymopapain M and papain

M P Thomas, C M Topham, D Kowlessur, G W Mellor, E W Thomas, D Whitford, K Brocklehurst
Journal: Biochemical Journal
Biochem J (1994) 300 (3): 805–820.
https://doi.org/10.1042/bj3000805
Published: 15 June 1994
View article titled, Structure of chymopapain M the late-eluted chymopapain deduced by comparative modelling techniques and active-centre characteristics determined by pH-dependent kinetics of catalysis and reactions with time-dependent inhibitors: the Cys-25/His-159 ion-pair is insufficient for catalytic competence in both chymopapain M and papain
Open the PDF for Structure of chymopapain M the late-eluted chymopapain deduced by comparative modelling techniques and active-centre characteristics determined by pH-dependent kinetics of catalysis and reactions with time-dependent inhibitors: the Cys-25/His-159 ion-pair is insufficient for catalytic competence in both chymopapain M and papain in another window
Articles

Clarification of the pH-dependent kinetic behaviour of papain by using reactivity probes and analysis of alkylation and catalysed acylation reactions in terms of multihydronic state models: implications for electrostatics calculations and interpretation of the consequences of site-specific mutations such as Asp-158-Asn and Asp-158-Glu

G W Mellor, M Patel, E W Thomas, K Brocklehurst
Journal: Biochemical Journal
Biochem J (1993) 294 (1): 201–210.
https://doi.org/10.1042/bj2940201
Published: 15 August 1993
View article titled, Clarification of the pH-dependent kinetic behaviour of papain by using reactivity probes and analysis of alkylation and catalysed acylation reactions in terms of multihydronic state models: implications for electrostatics calculations and interpretation of the consequences of site-specific mutations such as Asp-158-Asn and Asp-158-Glu
Open the PDF for Clarification of the pH-dependent kinetic behaviour of papain by using reactivity probes and analysis of alkylation and catalysed acylation reactions in terms of multihydronic state models: implications for electrostatics calculations and interpretation of the consequences of site-specific mutations such as Asp-158-Asn and Asp-158-Glu in another window
Articles

Catalytic-site characteristics of the porcine calpain II 80 kDa/18 kDa heterodimer revealed by selective reaction of its essential thiol group with two-hydronic-state time-dependent inhibitors: evidence for a catalytic site Cys/His interactive system and an ionizing modulatory group

G W Mellor, S K Sreedharan, D Kowlessur, E W Thomas, K Brocklehurst
Journal: Biochemical Journal
Biochem J (1993) 290 (1): 75–83.
https://doi.org/10.1042/bj2900075
Published: 15 February 1993
View article titled, Catalytic-site characteristics of the porcine calpain II 80 kDa/18 kDa heterodimer revealed by selective reaction of its essential thiol group with two-hydronic-state time-dependent inhibitors: evidence for a catalytic site Cys/His interactive system and an ionizing modulatory group
Open the PDF for Catalytic-site characteristics of the porcine calpain II 80 kDa/18 kDa heterodimer revealed by selective reaction of its essential thiol group with two-hydronic-state time-dependent inhibitors: evidence for a catalytic site Cys/His interactive system and an ionizing modulatory group in another window
Articles

Ionization characteristics of the Cys-25/His-159 interactive system and of the modulatory group of papain: resolution of ambiguity by electronic perturbation of the quasi-2-mercaptopyridine leaving group in a new pyrimidyl disulphide reactivity probe

G W Mellor, E W Thomas, C M Topham, K Brocklehurst
Journal: Biochemical Journal
Biochem J (1993) 290 (1): 289–296.
https://doi.org/10.1042/bj2900289
Published: 15 February 1993
View article titled, Ionization characteristics of the Cys-25/His-159 interactive system and of the modulatory group of papain: resolution of ambiguity by electronic perturbation of the quasi-2-mercaptopyridine leaving group in a new pyrimidyl disulphide reactivity probe
Open the PDF for Ionization characteristics of the Cys-25/His-159 interactive system and of the modulatory group of papain: resolution of ambiguity by electronic perturbation of the quasi-2-mercaptopyridine leaving group in a new pyrimidyl disulphide reactivity probe in another window
Articles

Evaluation of hydrogen-bonding and enantiomeric P 2 -S 2 hydrophobic contacts in dynamic aspects of molecular recognition by papain

M Patel, I S Kayani, W Templeton, G W Mellor, E W Thomas, K Brocklehurst
Journal: Biochemical Journal
Biochem J (1992) 287 (3): 881–889.
https://doi.org/10.1042/bj2870881
Published: 01 November 1992
View article titled, Evaluation of hydrogen-bonding and enantiomeric P 2 -S 2 hydrophobic contacts in dynamic aspects of molecular recognition by papain
Open the PDF for Evaluation of hydrogen-bonding and enantiomeric P 2 -S 2 hydrophobic contacts in dynamic aspects of molecular recognition by papain in another window
Articles

Variation in the P 2 -S 2 stereochemical selectivity towards the enantiomeric N -acetylphenylalanylglycine 4-nitroanilides among the cysteine proteinases papain, ficin and actinidin

M Patel, I S Kayani, G W Mellor, S Sreedharan, W Templeton, E W Thomas, M Thomas, K Brocklehurst
Journal: Biochemical Journal
Biochem J (1992) 281 (2): 553–559.
https://doi.org/10.1042/bj2810553
Published: 15 January 1992
View article titled, Variation in the P 2 -S 2 stereochemical selectivity towards the enantiomeric N -acetylphenylalanylglycine 4-nitroanilides among the cysteine proteinases papain, ficin and actinidin
Open the PDF for Variation in the P 2 -S 2 stereochemical selectivity towards the enantiomeric N -acetylphenylalanylglycine 4-nitroanilides among the cysteine proteinases papain, ficin and actinidin in another window
Articles

Structure-function relationships in the cysteine proteinases actinidin, papain and papaya proteinase Ω. Three-dimensional structure of papaya proteinase Ω deduced by knowledge-based modelling and active-centre characteristics determined by two-hydronic-state reactivity probe kinetics and kinetics of catalysis

C M Topham, E Salih, C Frazao, D Kowlessur, J P Overington, M Thomas, S M Brocklehurst, M Patel, E W Thomas, K Brocklehurst
Journal: Biochemical Journal
Biochem J (1991) 280 (1): 79–92.
https://doi.org/10.1042/bj2800079
Published: 15 November 1991
View article titled, Structure-function relationships in the cysteine proteinases actinidin, papain and papaya proteinase Ω. Three-dimensional structure of papaya proteinase Ω deduced by knowledge-based modelling and active-centre characteristics determined by two-hydronic-state reactivity probe kinetics and kinetics of catalysis
Open the PDF for Structure-function relationships in the cysteine proteinases actinidin, papain and papaya proteinase Ω. Three-dimensional structure of papaya proteinase Ω deduced by knowledge-based modelling and active-centre characteristics determined by two-hydronic-state reactivity probe kinetics and kinetics of catalysis in another window
Articles

A re-appraisal of the structural basis of stereochemical recognition in papain. Insensitivity of binding-site-catalytic-site signalling to P 2 -chirality in a time-dependent inhibition

W Templeton, D Kowlessur, E W Thomas, C M Topham, K Brocklehurst
Journal: Biochemical Journal
Biochem J (1990) 266 (3): 645–651.
https://doi.org/10.1042/bj2660645
Published: 15 March 1990
View article titled, A re-appraisal of the structural basis of stereochemical recognition in papain. Insensitivity of binding-site-catalytic-site signalling to P 2 -chirality in a time-dependent inhibition
Open the PDF for A re-appraisal of the structural basis of stereochemical recognition in papain. Insensitivity of binding-site-catalytic-site signalling to P 2 -chirality in a time-dependent inhibition in another window
Articles

Dependence of the P 2 -S 2 stereochemical selectivity of papain on the nature of the catalytic-site chemistry. Quantification of selectivity in the catalysed hydrolysis of the enantiomeric N -acetylphenylalanylglycine 4-nitroanilides

D Kowlessur, E W Thomas, C M Topham, W Templeton, K Brocklehurst
Journal: Biochemical Journal
Biochem J (1990) 266 (3): 653–660.
https://doi.org/10.1042/bj2660653
Published: 15 March 1990
View article titled, Dependence of the P 2 -S 2 stereochemical selectivity of papain on the nature of the catalytic-site chemistry. Quantification of selectivity in the catalysed hydrolysis of the enantiomeric N -acetylphenylalanylglycine 4-nitroanilides
Open the PDF for Dependence of the P 2 -S 2 stereochemical selectivity of papain on the nature of the catalytic-site chemistry. Quantification of selectivity in the catalysed hydrolysis of the enantiomeric N -acetylphenylalanylglycine 4-nitroanilides in another window
Articles

The interplay of electrostatic fields and binding interactions determining catalytic-site reactivity in actinidin. A possible origin of differences in the behaviour of actinidin and papain

D Kowlessur, M O'Driscoll, C M Topham, W Templeton, E W Thomas, K Brocklehurst
Journal: Biochemical Journal
Biochem J (1989) 259 (2): 443–452.
https://doi.org/10.1042/bj2590443
Published: 15 April 1989
View article titled, The interplay of electrostatic fields and binding interactions determining catalytic-site reactivity in actinidin. A possible origin of differences in the behaviour of actinidin and papain
Open the PDF for The interplay of electrostatic fields and binding interactions determining catalytic-site reactivity in actinidin. A possible origin of differences in the behaviour of actinidin and papain in another window
Articles

Identification of signalling and non-signalling binding contributions to enzyme reactivity. Alternative combinations of binding interactions provide for change in transition-state geometry in reactions of papain

D Kowlessur, C M Topham, E W Thomas, M O'Driscoll, W Templeton, K Brocklehurst
Journal: Biochemical Journal
Biochem J (1989) 258 (3): 755–764.
https://doi.org/10.1042/bj2580755
Published: 15 March 1989
View article titled, Identification of signalling and non-signalling binding contributions to enzyme reactivity. Alternative combinations of binding interactions provide for change in transition-state geometry in reactions of papain
Open the PDF for Identification of signalling and non-signalling binding contributions to enzyme reactivity. Alternative combinations of binding interactions provide for change in transition-state geometry in reactions of papain in another window
Articles

The interplay of electrostatic and binding interactions determining active centre chemistry and catalytic activity in actinidin and papain

K Brocklehurst, M O'Driscoll, D Kowlessur, I R Phillips, W Templeton, E W Thomas, C M Topham, C W Wharton
Journal: Biochemical Journal
Biochem J (1989) 257 (1): 309–310.
https://doi.org/10.1042/bj2570309
Published: 01 January 1989
View article titled, The interplay of electrostatic and binding interactions determining active centre chemistry and catalytic activity in actinidin and papain
Open the PDF for The interplay of electrostatic and binding interactions determining active centre chemistry and catalytic activity in actinidin and papain in another window
Articles

Consequences of molecular recognition in the S 1 -S 2 intersubsite region of papain for catalytic-site chemistry. Change in pH-dependence characteristics and generation of an inverse solvent kinetic isotope effect by introduction of a P 1 -P 2 amide bond into a two-protonic-state reactivity probe

K Brocklehurst, D Kowlessur, G Patel, W Templeton, K Quigley, E W Thomas, C W Wharton, F Willenbrock, R J Szawelski
Journal: Biochemical Journal
Biochem J (1988) 250 (3): 761–772.
https://doi.org/10.1042/bj2500761
Published: 15 March 1988
View article titled, Consequences of molecular recognition in the S 1 -S 2 intersubsite region of papain for catalytic-site chemistry. Change in pH-dependence characteristics and generation of an inverse solvent kinetic isotope effect by introduction of a P 1 -P 2 amide bond into a two-protonic-state reactivity probe
Open the PDF for Consequences of molecular recognition in the S 1 -S 2 intersubsite region of papain for catalytic-site chemistry. Change in pH-dependence characteristics and generation of an inverse solvent kinetic isotope effect by introduction of a P 1 -P 2 amide bond into a two-protonic-state reactivity probe in another window
Articles

Substrate-derived two-protonic-state electrophiles as sensitive kinetic specificity probes for cysteine proteinases. Activation of 2-pyridyl disulphides by hydrogen-bonding

K Brocklehurst, D Kowlessur, M O'Driscoll, G Patel, S Quenby, E Salih, W Templeton, E W Thomas, F Willenbrock
Journal: Biochemical Journal
Biochem J (1987) 244 (1): 173–181.
https://doi.org/10.1042/bj2440173
Published: 15 May 1987
View article titled, Substrate-derived two-protonic-state electrophiles as sensitive kinetic specificity probes for cysteine proteinases. Activation of 2-pyridyl disulphides by hydrogen-bonding
Open the PDF for Substrate-derived two-protonic-state electrophiles as sensitive kinetic specificity probes for cysteine proteinases. Activation of 2-pyridyl disulphides by hydrogen-bonding in another window
Articles

Purification of an NADH-(dichlorophenol-indophenol) oxidoreductase from Bacillus stearothermophilus

I Mains, D M Power, E W Thomas, J A Buswell
Journal: Biochemical Journal
Biochem J (1980) 191 (2): 457–465.
https://doi.org/10.1042/bj1910457
Published: 01 November 1980
View article titled, Purification of an NADH-(dichlorophenol-indophenol) oxidoreductase from Bacillus stearothermophilus
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Articles

Affinity chromatography of pig heart fumarase

S Chaudhuri, E W Thomas
Journal: Biochemical Journal
Biochem J (1979) 177 (1): 115–119.
https://doi.org/10.1042/bj1770115
Published: 01 January 1979
View article titled, Affinity chromatography of pig heart fumarase
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