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(trafficking AND membrane)

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Biochem J (2021) 478 (7): 1315–1319.
Published: 06 April 2021
... (2): 407–422) of complexin-2 as an important contributor to glucose transporter 4 (GLUT4) translocation to muscle cell plasma membrane upon insulin stimulation is essential. The present commentary discusses the biological importance of the findings and proposes future challenges and opportunities...
Articles
Biochem J (2021) 478 (10): 1977–1984.
Published: 28 May 2021
... regulation was also reported in mutants affected in mitochondrial respiration as rpoTmp (defective in the T7-phage-type organellar RNA polymerase shared by plastids and mitochondria) and atphB3 (defective in the mitochondrial membrane protein PROHIBITIN 3), presenting strong induction of oxidative stress...
Articles
Biochem J (2021) 478 (19): 3643–3654.
Published: 14 October 2021
...Daniel Wirth; Ece Özdemir; Christopher King; Lena Ahlswede; Dirk Schneider; Kalina Hristova The spatial distribution of proteins in cell membranes is crucial for signal transduction, cell communication and membrane trafficking. Members of the Tetraspanin family organize functional protein clusters...
Articles
Biochem J (2021) 478 (2): 407–422.
Published: 29 January 2021
... al. ( 2006 ) Molecular dissection of the Munc18c/syntaxin4 interaction: implications for regulation of membrane trafficking . Traffic 7 , 1408 – 1419 10.1111/j.1600-0854.2006.00474.x 14 Widberg , C.H. , Bryant , N.J. , Girotti , M. , Rea , S. and James , D.E...
Includes: Supplementary data
Articles
Biochem J (2021) 478 (10): 1959–1976.
Published: 28 May 2021
... fusion [ 13 ]. The conflicting observations are likely attributed to differential regulation of the binding partners upon autophagy induction in response to distinct stimuli. Additionally, VAMP3/Cellubrevin has been indicated to play a role in membrane trafficking in non-neuronal cells. Studies in K562...
Articles
Biochem J (2021) 478 (12): 2339–2357.
Published: 23 June 2021
... interfering RNA (siRNA), we generated CLN5 knockdown (CLN5 KD ) HeLa cells. In these cells, we found less membrane bound RAB7A [ 7 ]. RAB7A is a small GTPases that can bind to and recruit retromer, a protein complex that mediates endosome-to-trans Golgi network (TGN) trafficking. Once recruited, retromer...
Includes: Supplementary data
Articles
Biochem J (2021) 478 (22): 3977–3998.
Published: 23 November 2021
... endosome (ERC/RE) and the Trans Golgi Network (TGN) and is required for recycling processes directed towards the plasma membrane, cytokinesis and primary cilia formation [ 145 ]. It also acts in a cascade with Rab8a, a post-Golgi network-localized GTPase involved in several trafficking pathways, including...
Articles
Biochem J (2021) 478 (24): 4203–4220.
Published: 23 December 2021
...-type CFTR into F508del CFTR-expressing cells increases SLC26A9 trafficking to the plasma membrane [ 38 ]. Together, these results reveal that F508del CFTR prevents SLC26A9 trafficking to the plasma membrane and increases the degradation of ER-retained SLC26A9. Although a significant body of work...
Includes: Supplementary data
Articles
Biochem J (2021) 478 (3): 553–578.
Published: 10 February 2021
... mutations in LRRK2 that enhance kinase activity cause Parkinson's disease. LRRK2 phosphorylates a subset of Rab GTPases including Rab8A and Rab10 within its effector binding motif. Here, we explore whether LRRK1, a less studied homolog of LRRK2 that regulates growth factor receptor trafficking...
Includes: Supplementary data
Articles
Biochem J (2021) 478 (6): 1261–1282.
Published: 19 March 2021
...Diti Chatterjee Bhowmick; Lydia Burnett; Zhanar Kudaibergenova; Aleksandar M. Jeremic Here, we investigated transcriptional and trafficking mechanisms of human islet amyloid polypeptide (hIAPP) in normal and stressed β-cells. In high glucose-challenged human islets and rat insulinoma cells...
Includes: Multimedia, Supplementary data
Articles
Biochem J (2021) 478 (8): 1617–1629.
Published: 28 April 2021
... vasopressin-independent aquaporin-2 water channel trafficking to the apical membranes of cells lining the kidney collecting ducts [ 92 ]. AKAP220 has been found to participate in mobilizing a microtubule-associated network in the leading edge of cells by integrating cAMP and calcium signals...
Articles
Biochem J (2021) 478 (1): 21–39.
Published: 08 January 2021
... to be led by GBM stem cells (GSCs). Also, tumor networking and intercellular communication play a major role in driving GBM therapy-resistance. Tunneling Nanotubes (TNTs), thin membranous open-ended channels connecting distant cells, have been observed in several types of cancer, where they emerge to drive...
Includes: Multimedia, Supplementary data
Articles
Biochem J (2021) 478 (1): 261–279.
Published: 15 January 2021
..., such as proteasomal degradation, signal transduction, and protein trafficking. The process is also involved in events for establishing viral infection and replication. The first step in ubiquitination involves ubiquitin (Ub) binding with Ub-activating enzyme (E1, also termed UBE1) via a thioester linkage. Our results...
Articles
Biochem J (2021) 478 (11): 2051–2057.
Published: 08 June 2021
... protein production and trafficking to the plasma membrane but have no transport function, whereas others suffer from protein expression, folding and trafficking defects, and thus the mutant proteins fail to reach the plasma membrane [ 21–24 ]. To understand full well the consequences of the different...
Articles
Biochem J (2021) 478 (14): 2921–2925.
Published: 28 July 2021
... (not depicted in the figure). A proteinaceous pore could also be formed by syntaxins localized to the cell surface (e.g. syntaxin1) ( C ), to directly mediate tau transport across the plasma membrane [ 14 ]. On the other hand, tau may exploit interactions with MVB-specific SNAREs (e.g. STX8 and VAMP8) to get...
Articles
Biochem J (2021) 478 (1): 197–215.
Published: 15 January 2021
... of the ferlin family. This group of proteins is involved in calcium-sensitive membrane trafficking and comprises six members including dysferlin, myoferlin, otoferlin, Fer1L4, Fer1L5, and Fer1L6. Dysferlin has been shown to be an important membrane repair protein. Knockout experiments show that mice lacking...
Articles
Biochem J (2021) 478 (18): 3429–3444.
Published: 23 September 2021
...Pradeep Kumar Sheokand; Monika Narwal; Vandana Thakur; Asif Mohmmed Phospholipid synthesis is crucial for membrane proliferation in malaria parasites during the entire cycle in the host cell. The major phospholipid of parasite membranes, phosphatidylcholine (PC), is mainly synthesized through...
Includes: Multimedia, Supplementary data
Articles
Biochem J (2021) 478 (18): 3395–3421.
Published: 23 September 2021
... sites within phagophore membrane by WIPI2 [ 121 ]. In summary, elongation is facilitated by the ATG12–ATG5–ATG16L1 complex inserting lipidated LC3 (LC3-II) into phagophores and ATG9A-dependent membrane trafficking to growing phagophore membranes. Stage 4 Autophagosome Maturation : Temporally...
Articles
Biochem J (2021) 478 (8): 1605–1615.
Published: 28 April 2021
... in the mechanisms by which insulin regulates the translocation of GLUT4 to the plasma membrane in muscle, indicating a role for this in mechanisms regulating vesicle trafficking in cells [ 10 ]. We have shown that glucose induces phosphorylation of β-catenin on Ser552 but not the Ser675 site in β-cells via...
Articles
Biochem J (2021) 478 (9): 1705–1732.
Published: 10 May 2021
... Plasmodium falciparum PROPPIN Malaria parasites undergo a multi-stage development in diverse intracellular and extracellular environments. Several of these stages are specialized for invasion, degradation of host cellular contents to obtain nutrients, generation of trafficking systems for import...
Includes: Supplementary data
Articles
Biochem J (2021) 478 (16): 3099–3123.
Published: 26 August 2021
... released from the membrane and into the cell, the clathrin coat is disassembled. Removal of the clathrin coat allows the vesicle to be trafficked to the early endosome and CME adaptors associated with the clathrin coat to be recycled. Synaptojanin and Oculocerebrorenal syndrome of Lowe inositol...
Articles
Biochem J (2021) 478 (7): 1471–1484.
Published: 16 April 2021
...-2001-2-11-reviews3012 32 Jung , J.J. , Inamdar , S.M. , Tiwari , A. and Choudhury , A. ( 2012 ) Regulation of intracellular membrane trafficking and cell dynamics by syntaxin-6 . Biosci. Rep. 32 , 383 – 391 10.1042/BSR20120006 33 Yamada , K. , Holth , J.K...
Includes: Supplementary data
Articles
Biochem J (2021) 478 (6): 1199–1225.
Published: 19 March 2021
... of PI3Ks: class I PI3Ks produce PIP 3 at plasma membrane level. Although D. melanogaster and C. elegans have only one form of class I PI3K, vertebrates have four class I PI3Ks called isoforms despite being encoded by four different genes. Hence, duplication of these genes coincides with the acquisition...
Includes: Supplementary data
Articles
Biochem J (2021) 478 (3): 633–646.
Published: 12 February 2021
...-mediated phosphorylation, thereby acting to link increased insulin signaling to trafficking/fusion of vesicles containing the insulin-sensitive glucose transporter type 4 (GLUT4) to the plasma membrane [ 23 ]. Phosphorylation of AS160 in response to insulin also increases its association with the scaffold...
Includes: Supplementary data
Articles
Biochem J (2021) 478 (14): 2733–2758.
Published: 23 July 2021
... of these assemblies may be causative towards the disruption of intra- and intercellular membrane trafficking, leading to the downstream weakening of synaptic communications between neurons [ 4 ]. In many NDs, mitochondrial health is also compromised, leading to impaired bioenergetics, metabolism, and oxidative stress...
Articles
Biochem J (2021) 478 (8): 1515–1524.
Published: 21 April 2021
.... , Saalbach , G. , Larsson , C. and Kjellbom , P. ( 2004 ) Arabidopsis plasma membrane proteomics identifies components of transport, signal transduction and membrane trafficking . Plant Cell Physiol. 45 , 1543 – 1556 10.1093/pcp/pch209 62 Marmagne , A. , Ferro , M...
Articles
Biochem J (2021) 478 (6): 1303–1307.
Published: 23 March 2021
... of our review articles [ 37 ]. PI3K-produced PIP3 activates phosphoinositide-dependent protein kinase-1 (PDK1), which subsequently activates AKT (termed PKB in the U.K.) at the plasma membrane. AKT signals connect to mTOR (see Figure 1 ) by phosphorylating tuberous sclerosis complex 2 (TSC2) GTPase...
Articles
Biochem J (2021) 478 (14): 2811–2823.
Published: 23 July 2021
...Sebastian Mathea; Eidarus Salah; Cynthia Tallant; Deep Chatterjee; Benedict-Tilman Berger; Rebecca Konietzny; Susanne Müller; Benedikt M. Kessler; Stefan Knapp The human protein kinase ULK3 regulates the timing of membrane abscission, thus being involved in exosome budding and cytokinesis. Herein...
Includes: Supplementary data
Articles
Biochem J (2021) 478 (3): 463–486.
Published: 05 February 2021
... a transformative agreement with EBSCO. blood-brain barrier brain EIEE25/DEE25 liver metabolic syndrome NACT/SLC13A5/mINDY Transport proteins (transporters) are involved in the movement of several biological molecules (e.g. nutrients, metabolites) across the plasma membrane and membranes...
Articles
Biochem J (2021) 478 (1): 247–260.
Published: 15 January 2021
... ]. Early mucosal restitution after wounding refers to epithelial cell migration into a defect in a process independent of epithelial cell proliferation [ 2 , 3 ]. Cell migration is a complex process that involves cytoskeletal changes, matrix adhesion sites and membrane trafficking in response to stress [ 4...
Articles
Biochem J (2021) 478 (2): 341–355.
Published: 27 January 2021
... and also cell death and senescence. PKC isozymes were initially studied in cancer following their identification as the ‘receptors’ for tumour promoting phorbol esters. These compounds embed in cell membranes and mimic binding of the natural agonist diacylglycerol (DAG) to acutely activate PKC [ 1 , 2...
Articles
Biochem J (2021) 478 (12): 2309–2319.
Published: 23 June 2021
... secreted proteins, are first synthesized in the endoplasmic reticulum (ER), but only 5–20% of each lysosomal enzyme synthesized is secreted directly outside the cell via the secretory pathway, while the rest are trafficked to lysosomes [ 13 ]. Altering the native signal peptide sequence, therefore, offers...
Includes: Supplementary data
Articles
Biochem J (2021) 478 (15): 2977–2997.
Published: 10 August 2021
... carboxylase-1 (ACC1) [ 6 , 7 ]. The activation of AMPK also leads to increased fatty acid oxidation through phosphorylation of ACC2 [ 8 ], and glucose uptake in skeletal muscle, at least in part through phosphorylation of TBC1D1 [ 9 , 10 ], a Rab GTPase-activating protein (GAP) which modulates trafficking...
Includes: Supplementary data
Articles
Biochem J (2021) 478 (2): 357–375.
Published: 27 January 2021
...-sensing systems for rapid adaptation to changes in the nutrient supply, including several types of plasma membrane nutrient sensors [ 1 ]. The latter include the glucose/sucrose-sensing G-protein coupled receptor (GPCR) Gpr1 [ 2 ] and the transporter-like, but transport-deficient glucose sensors, Snf3...
Includes: Supplementary data
Articles
Biochem J (2021) 478 (19): 3613–3619.
Published: 08 October 2021
... database for potential methyllysine readers. Of the over 60 hits, the most interesting emerged as possible regulators of various metabolite and carbohydrate processing systems, ion transport, membrane trafficking and cellular signaling ( Supplementary Table S1 and Figure 2c ) Interestingly, some of hits...
Includes: Supplementary data
Articles
Biochem J (2021) 478 (2): 443–461.
Published: 29 January 2021
... by Portland Press Limited on behalf of the Biochemical Society 2021 liquid consensates phase separation steroid receptors transcripcion factors Steroid receptors (SRs) are ligand-activatable transcription factors (TFs) that bind membrane-permeable steroid hormones and control gene expression...
Articles
Biochem J (2021) 478 (10): 1861–1877.
Published: 18 May 2021
... [ 27 ]. It functions as an ACSL with a specificity for activation of long-chain and very-long-chain fatty acids (VLFA) for the syntheses of polar and neutral lipids as well as β-oxidation [ 25–27 ]. It has been shown that GPLs and neutral lipids can be regulated by ER-Golgi trafficking [ 28...
Includes: Supplementary data
Articles
Biochem J (2021) 478 (5): 1009–1021.
Published: 04 March 2021
..., and in the extracellular and pericellular matrices [ 2 , 3 ]. Cell surface PGs either span the membrane or anchored via glycosylphosphatidylinositol. Extracellular and pericellular PGs are secreted and exist as macromolecular complexes with other PGs and proteins. In addition, PG ectodomains and GAGs are cleaved...
Includes: Supplementary data
Articles
Biochem J (2021) 478 (14): 2759–2774.
Published: 23 July 2021
.... Genet. 5 , 270 10.3389/fgene.2014.00270 4 Offringa , R. and Huang , F. ( 2013 ) Phosphorylation-dependent trafficking of plasma membrane proteins in animal and plant cells . J. Integr. Plant Biol. 55 , 789 – 808 10.1111/jipb.12096 5 Humphrey , S.J. , James...
Articles
Biochem J (2021) 478 (13): 2715–2732.
Published: 16 July 2021
... The nuclear envelope presented in the eukaryotic cells establishes an essential barrier to controlling and regulating cellular processes, such as gene expression and cell cycle evolution. The transport of the most well-characterized components through the nuclear membrane occurs after the recognition...
Articles
Biochem J (2021) 478 (7): 1359–1375.
Published: 16 April 2021
... ]. It is an ancient and evolutionarily conserved catabolic process and is intensely involved in the degradation and recycling of cytoplasmic products. Essentially, autophagy is a trafficking pathway. It transports cytoplasmic cargo to the lysosome for their subsequent degradation via lysosomal hydrolases. Cytosolic...
Articles
Biochem J (2021) 478 (12): 2321–2337.
Published: 23 June 2021
... membrane and microtubules are well documented. The microtubule networks determine the spatial patterning of the cytoplasm, the coupling of microtubules to membranes controls the structure and positioning of organelles and directs membrane trafficking between the organelles [ 19 ]. In addition to integral...
Includes: Multimedia, Supplementary data
Articles
Biochem J (2021) 478 (19): 3621–3642.
Published: 14 October 2021
... protein Bax [ 57 ], which can activate CerS in outer mitochondrial membrane [ 58 ]. The pro-apoptotic protein Bcl-2 homologous antagonist/killer (BAK) acts upstream of ceramide leading to activation of CerS, with the subsequent activation of PP2A, dephosphorylation, and inactivation of anti-apoptotic Bcl...
Articles
Biochem J (2021) 478 (8): 1525–1545.
Published: 21 April 2021
... contains an amphipathic helix (termed H1, comprising residues 15–22 in LAI; Figure 1A ) that helps to anchor Nef to the charged lipid headgroups of the cellular membrane [ 23 ]. In structural studies of full-length Nef, this H1 helix has been observed to bind to the hydrophobic groove formed by the core...
Includes: Supplementary data
Articles
Biochem J (2021) 478 (5): 1159–1173.
Published: 12 March 2021
... to the cellular degradation pathways, recent studies have revealed that lysosomes play active roles in nutrient sensing, adaption to multiple cellular stress, plasma membrane repair, cell signaling, and membrane trafficking [ 41 ]. Hence, lysosomal dysfunction has been associated to many diseases i.e. lysosomal...
Articles
Biochem J (2021) 478 (19): 3539–3553.
Published: 06 October 2021
... to pump blood throughout the body [ 1 ]. During E-C coupling, efficient, uniform and synchronous Ca 2+ release throughout the cytosol of cardiomyocytes is governed by a process called Ca 2+ -induced Ca 2+ release [ 2–4 ]. Cardiomyocytes have developed intracellular membrane junctional contacts...
Articles
Biochem J (2021) 478 (24): 4153–4167.
Published: 16 December 2021
... stained with SimplyBlue SafeStain (Invitrogen, U.K.). SDS–PAGE gels were transferred to a nitrocellulose membrane (BioTrace, Pall Life Sciences) at 100 V for 1 h in low molecular weight transfer buffer (25 mM Tris, 192 mM glycine, 40% methanol) in a Mini Trans-Blot Electrophoretic Transfer Cell (Bio-Rad...
Includes: Supplementary data
Articles
Biochem J (2021) 478 (23): 4099–4118.
Published: 06 December 2021
... by the finding that mutations in key components of the mitophagy pathway such as PINK1 or PRKN (Parkin) can cause familial autosomal recessive Parkinsonism [ 12 , 13 ]. Mitophagy is a multi-step process that ensures the removal of damaged mitochondria (e.g. those with loss of membrane potential) through...
Includes: Supplementary data
Articles
Biochem J (2021) 478 (12): 2179–2199.
Published: 18 June 2021
... complex was originally identified as a chaperoning complex that prevents tail-anchored proteins from aggregation prior to their proper insertion into the membrane [ 17–19 ]. By binding to hydrophobic or misfolded proteins the BAG6 complex assists also in proteasome-mediated degradation [ 20 , 21...
Articles
Biochem J (2021) 478 (8): 1647–1661.
Published: 30 April 2021
... in the regulation of many cellular processes, including energy and phosphate metabolism, DNA repair, ribosome biogenesis, vesicle trafficking and insulin secretion [ 1–5 ]. In mammals, the most abundant inositol pyrophosphate is 5-diphosphoinositol pentakisphosphate (5-PP-IP5 or 5-IP 7 ), which is synthesized from...
Includes: Supplementary data