... energy/ATP required for cell migration during the initial stage of microvascularization. Extracellularly, the physiological energy-rich polymer, inorganic polyphosphate (polyP), applied as biomimetic amorphous calcium polyP microparticles (Ca-polyP-MP), is functioning as a substrate for ATP generation...

... also be linked. In HaCaT keratinocytes, ATP caused a rapid and strong but transient activation of hyaluronan synthase 2 ( HAS2 ) expression via protein kinase C-, Ca 2+ /calmodulin-dependent protein kinase II-, mitogen-activated protein kinase-, and calcium response element-binding protein-dependent...

...Michael F. Jackson Pannexin channels are recognized as important conduits for the release of ATP, which contributes to purinergic signalling. Pathologically, ATP release via these channels acts as a find-me signal for apoptotic cell clearance. Accordingly, there is considerable and growing interest...

..., whereas it is stronger when Nrf2 activity is constitutively high. In the absence of glucose, addition of fatty acids differentially affects the production of ATP in mouse embryonic fibroblasts from wild-type, Nrf2-knockout and Keap1 (Kelch-like ECH-associated protein 1)-knockout mice. In acute tissue...

...Katarzyna Hanzelka; Lukasz Skalniak; Jolanta Jura; Sigurd Lenzen; Ewa Gurgul-Convey Mimitin, a novel mitochondrial protein, has been shown to act as a molecular chaperone for the mitochondrial complex I and to regulate ATP synthesis. During Type 1 diabetes development, pro-inflammatory cytokines...

... encoding all three cytosolic domains of GLUT4 [GST (glutathione-transferase)–GLUT4-cyto]. Both proteins bound to a lesser extent the middle cytosolic loop but not individual N- or C-terminal domains of GLUT4. Purified GAPDH and HKII competed for binding to GST–GLUT4-cyto; ATP increased GAPDH binding...

...Mariko Omatsu-Kanbe; Kazuko Inoue; Yusuke Fujii; Takefumi Yamamoto; Takahiro Isono; Norihisa Fujita; Hiroshi Matsuura The effect of extracellular ATP on adipogenesis was investigated using the mouse 3T3-L1 cell line. Incubation of cells with ATP (1–100 μM) for 5 min induced actin filament...

... that the muscarinic agonist carbachol produced a rapid, simultaneous and reversible cessation of the movements of both organelles, which was dependent on a rise in cytosolic Ca 2+ . This rise in Ca 2+ was shown to cause a fall in cellular ATP levels, and the effect of carbachol on organelle movement could be mimicked...

... was prevented by the prior addition of ADPR and vice versa, indicating that both compounds share some mechanisms mediating the rise in [Ca 2+ ] i . NAD + , as well as ADPR, were ineffective when applied following ATP, suggesting that ATP controls events that intersect with NAD + and ADPR signalling. 1...

...Schuichi KOIZUMI; Kayoko FUJISHITA; Kaori INOUE; Yukari SHIGEMOTO-MOGAMI; Makoto TSUDA; Kazuhide INOUE ATP acts as an intercellular messenger in a variety of cells. In the present study, we have characterized the propagation of Ca 2+ waves mediated by extracellular ATP in cultured NHEKs (normal...

... of thapsigargin-stimulated Ca 2+ inflow, a 40% inhibition of ATP and maitotoxin-stimulated Ca 2+ inflow, and a 50% inhibition of maitotoxin-stimulated Mn 2+ inflow. The idea that, in liver cells, TRPC1 encodes a non-selective cation channel involved directly or indirectly in the regulation of cell volume...

... a strong correlation between the catalytic efficiency of three family I PPases (from Saccharomyces cerevisiae , Escherichia coli and rat liver) and one family II PPase (from Streptococcus mutans ) in ATP and tripolyphosphate (P 3 ) hydrolysis in the presence of Mg 2+ , Mn 2+ , Zn 2+ and Co 2+ on the one...

...). In ghosts, ATP (1–4mM) competitively antagonizes oestradiol, genistein and cytochalasin B (CB)-dependent inhibitions of glucose exit, ( K i(ATP/ED) = 2.5±0.23mM, K i(ATP/GEN) = 0.99±0.17mM and K i(ATP/CB) = 0.76±0.08mM). Tamoxifen and faslodex reverse oestradiol-dependent inhibition of glucose exit with ATP...

... to bradykinin (BK) and ATP. Incubation of porcine aortic endothelial cells with BK (1 μ M) in the presence of 3mM extracellular Ca 2+ increased [Ca 2+ ] i from 110 to 350nM and enhanced the rate of l -[ 3 H]citrulline formation from 0.1 to 5fmol/min. In the absence of extracellular Ca 2+ , the BK-induced...

.... The M. tuberculosis DnaA protein binds the origin of replication ( oriC ), ATP and ADP, and exhibited weak ATPase activity. ADP, after hydrolysis of ATP, remained strongly associated with DnaA and the exchange of ATP for bound ADP was weak. Vesicles prepared from acidic phospholipids...

...Donald A. VESSEY; Michael KELLEY The XL-I form of xenobiotic/medium-chain fatty acid:CoA ligase was purified to apparent homogeneity from bovine liver mitochondria and used to determine the reaction mechanism. A tersubstrate kinetic analysis was conducted by varying the concentrations of ATP...

...Edward K. AINSCOW; Guy A. RUTTER Increases in mitochondrial [Ca 2+ ] ([Ca 2+ ] m ) have recently been reported to cause long-term alterations in cellular ATP production [Jouaville, Bastianutto, Rutter and Rizzuto (1999) Proc. Natl. Acad. Sci. U.S.A. 96 , 13807Ő13812]. We have determined...

.... In single HEK-293 cells, ATP and carbamylcholine (CCh) stimulated Ca 2+ release from intracellular stores via a pathway that was entirely dependent on Ins(1,4,5) P 3 . After stimulation with maximal concentrations of ATP or CCh in Ca 2+ -free medium, there was no response to a second stimulation...

...Susana CADENAS; Martin D. BRAND During oxidative phosphorylation most of the protons pumped out to the cytosol across the mitochondrial inner membrane return to the matrix through the ATP synthase, driving ATP synthesis. However, some of them leak back to the matrix through a proton-conductance...

...), endothelin-1 (ET-1) and ATP. In the present study we have used in vitro activity assays of purified eNOS and in vitro binding assays with glutathione S-transferase fusion proteins to show that the capacity to bind and inhibit eNOS is a common feature of membrane-proximal regions of intracellular domain 4...

..., energy metabolism and protein phosphorylation during apoptosis of neuronal PC12 cells induced by nerve growth factor and serum deprivation was examined using [ 32 P]P i -labelling techniques. Although ATP levels were maintained at control levels during apoptosis, [ 32 P]P i incorporation into ATP...