... polypeptide of the EccC enzyme contains two Ftsk/SpoIIIE type ATPase domains (D2 and D3) and binds to the EsxAB factor at the C-terminal region of the D3 domain. In the current study, we have determined a low-resolution structure of EccCb1, and its mechanism involved in ATPase activity and EsxAB factor...

... by Escherichia coli cells has been purified as a single heptameric ring. It possesses ATPase activity and does not require a co-chaperonin for its function. In vitro experiments demonstrated that gp246 is able to suppress the thermal protein inactivation and aggregation in an ATP-dependent manner, thus...

..., but at the expense of optimal folding efficiency, suggesting an evolutionary optimization of the co-chaperones for their Hsp70 partner. ATPase activity DnaJ DnaK GrpE heat-shock protein 70 escort protein (Hsp70 escort protein) protein folding Hsp70s (heat-shock proteins of 70 kDa) are a class...

.... Complementary to the in silico screening, a versatile and sensitive colorimetric assay was set up to measure the disruption of the ATPase activity of Pontin. This assay allowed the determination of inhibition curves for more than 20 top-scoring compounds, resulting in the identification of four ligands...

.... 1 To whom correspondence should be addressed (email [email protected] ). 27 6 2011 25 10 2011 17 11 2011 17 11 2011 © The Authors Journal compilation © 2012 Biochemical Society 2012 A13T mutation ATPase activity familial hypertrophic cardiomyopathy (FHC...

... ATPase activity cholesterol oxidase detergent-resistant membrane (DRM) leukotriene C 4 transport assay lipid raft multidrug-resistance-related protein 1 (MRP1) Protein from equal volumes of the gradient fractions was processed as described in [ 14 ]. Given their localization in DRMs...

... coupling of myosin, MyBPC1 and MM-CK is further corroborated using an ATPase activity assay in which ATP expenditure accelerates upon the association of the three proteins, and the apparent K m value of myosin is therefore reduced. The results of the present study suggest that MyBPC1 acts as an adaptor...

... (Hsp90) and positively discriminating against the first valine residue in the VEEVD motif (Ssa1). In the present study we also show that Tah1 can affect the ATPase activity of Hsp90, in common with some other TPR-domain proteins. 1 To whom correspondence should be addressed (email chris.prodromou...

...Ayana MOORE; Linda WORDEMAN Mitotic centromere-associated kinesin (MCAK) is a microtubule (MT)-destabilizing molecular motor. In the present study we show that the final 8 amino acids of the C-terminus of MCAK inhibit lattice-stimulated ATPase activity of the motor. Surprisingly, loss of this C...

... understanding of the regulatory mech- anism of scallop muscle myosin may shed light on the regulatory mechanism of both smooth muscle and the non-muscle myosins. The binding of calcium to molluscan myosin or to its double- headed proteolytic fragment, heavy meromyosin (HMM), increases the Mg-ATPase activity...

... to microtubules and severs them in an ATP-dependent manner in vitro . ATPase activity of Atp60 is stimulated by low tubulin/katanin ratios, and is inhibited at higher ratios. Considering its properties in vitro , several functions of Atp60 in vivo are discussed. Biochem. J. (2002) 365, 337 342 (Printed in Great...

... 2002 23 4 2002 The Biochemical Society, London ©2002 2002 ATPase activity calcium regulation enzyme kinetics heavy meromyosin (HMM) myosin Abbreviations used: HMM, heavy meromyosin; scHMM, HMM from scallop striated muscle; S1, myosin subfragment 1; scS1, scallop S1; mant...