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Divergent contribution of the MVA and MEP pathways to the formation of polyprenols and dolichols in Arabidopsis

Agata Lipko, Cezary Pączkowski, Laura Perez-Fons, Paul D. Fraser, Magdalena Kania, Marta Hoffman-Sommer, Witold Danikiewicz, Michel Rohmer, Jaroslaw Poznanski, Ewa Swiezewska

Biochem J (2023) 480 (8): 495–520.

https://doi.org/10.1042/BCJ20220578

Published: 21 April 2023

... access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) . Arabidopsis thaliana cross-talk dolichol MEP pathway MVA pathway polyprenol Isoprenoids constitute a very large...

View articletitled, Divergent contribution of the MVA and MEP pathways to the formation of polyprenols and dolichols in Arabidopsis

Includes: Supplementary data

Articles

An Arabidopsis thaliana arabinogalactan-protein (AGP31) and several cationic AGP fragments catalyse the boron bridging of rhamnogalacturonan-II

Dayan Sanhueza, Rifat Ara Begum, Cécile Albenne, Elisabeth Jamet, Stephen C. Fry

Biochem J (2022) 479 (18): 1967–1984.

https://doi.org/10.1042/BCJ20220340

Published: 23 September 2022

... natural chaperones remained elusive. We have now tested cationic peptide fragments of several Arabidopsis thaliana arabinogalactan-proteins (AGPs) as candidates. Fragments of AGP17, 18, 19 and 31 were effective, typically at ∼25 µg/ml (9–19 µM), promoting the boron bridging of 16–20 µM monomeric RG-II...

View articletitled, An Arabidopsis thaliana arabinogalactan-protein (AGP31) and several cationic AGP fragments catalyse the boron bridging of rhamnogalacturonan-II

Includes: Supplementary data

Articles

The Arabidopsis locus AT3G03890 encodes a dimeric β-barrel protein implicated in heme degradation

Jia Wang, Qi Guo, Xiaoyi Li, Xiao Wang, Lin Liu

Biochem J (2020) 477 (24): 4785–4796.

https://doi.org/10.1042/BCJ20200712

Published: 24 December 2020

... at 20 μM and HOZ concentration varied from 0 to 40 μM. The near-UV and visible range 300–800 nm were scanned. arabidopsis thaliana crystallography protein structure tetrapyrroles Heme is an essential pigment for plants and participates in various key biological processes [ 1...

View articletitled, The Arabidopsis locus AT3G03890 encodes a dimeric β-barrel protein implicated in heme degradation

Articles

Rubisco lysine acetylation occurs at very low stoichiometry in mature Arabidopsis leaves: implications for regulation of enzyme function

Brendan M. O'Leary, Andrew P. Scafaro, Ricarda Fenske, Owen Duncan, Elke Ströher, Jakob Petereit, A. Harvey Millar

Biochem J (2020) 477 (19): 3885–3896.

https://doi.org/10.1042/BCJ20200413

Published: 12 October 2020

... to confirm that Lys-acetylation at low stoichiometries can be a regulatory mechanism controlling Rubisco maximal activity. The results highlight the need for further use of stoichiometry measurements when determining the biological significance of reversible PTMs like acetylation. Arabidopsis thaliana...

View articletitled, Rubisco lysine acetylation occurs at very low stoichiometry in mature Arabidopsis leaves: implications for regulation of enzyme function

Includes: Supplementary data

Articles

Genetic activity during early plant embryogenesis

Ran Tian, Priyanka Paul, Sanjay Joshi, Sharyn E. Perry

Biochem J (2020) 477 (19): 3743–3767.

https://doi.org/10.1042/BCJ20190161

Published: 12 October 2020

... Arabidopsis thaliana (henceforth Arabidopsis ). In both ZE and SE, certain events must occur including the establishment of the body axis (polarity), and of the primary and apical meristems. As such, we briefly discuss key factors involved in the initiation of tissue systems during ZE, and speculate...

View articletitled, Genetic activity during early plant embryogenesis

Includes: Supplementary data

Articles

Three cytosolic NAD-malate dehydrogenase isoforms of Arabidopsis thaliana : on the crossroad between energy fluxes and redox signaling

Aleksandra Liszka, Regina Schimpf, Krupskaya Ivannova Cartuche Zaruma, Annika Buhr, Thorsten Seidel, Stefan Walter, Johannes Knuesting, Anna Dreyer, Karl-Josef Dietz, Renate Scheibe, Jennifer Selinski

Biochem J (2020) 477 (19): 3673–3693.

https://doi.org/10.1042/BCJ20200240

Published: 05 October 2020

... of the supplier (ThermoFisher Scientiﬁc, Darmstadt, Germany; RevertAid™ First Strand cDNA Synthesis Kit). Arabidopsis thaliana energy metabolism malate dehydrogenase redox signaling S-glutathionylation small molecules Malate dehydrogenases (MDHs) are essential components of shuttle systems...

View articletitled, Three cytosolic NAD-malate dehydrogenase isoforms of Arabidopsis thaliana : on the crossroad between energy fluxes and redox signaling

Includes: Supplementary data

Articles

The composition and turnover of the Arabidopsis thaliana 80S cytosolic ribosome

Karzan Jalal Salih, Owen Duncan, Lei Li, Josua Trösch, A. Harvey Millar

Biochem J (2020) 477 (16): 3019–3032.

https://doi.org/10.1042/BCJ20200385

Published: 26 August 2020

... in Arabidopsis thaliana by determining the abundance of different proteins during enrichment of ribosomes from cell cultures using peptide mass spectrometry. The turnover rates of 26 40S subunit r-proteins and 29 60S subunit r-proteins were also determined, showing that half of the ribosome population...

View articletitled, The composition and turnover of the Arabidopsis thaliana 80S cytosolic ribosome

Includes: Supplementary data

Articles

Biochemical and functional characterization of a mitochondrial citrate carrier in Arabidopsis thaliana

Biochem J (2020) 477 (9): 1759–1777.

https://doi.org/10.1042/BCJ20190785

Published: 15 May 2020

... The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2020 Arabidopsis thaliana citrate isocitrat mitochondrial carrier succinate/fumarate carrier 21 10 2019 22 4 2020 23 4 2020 24 4 2020 Correspondence: Adriano Nunes-Nesi...

View articletitled, Biochemical and functional characterization of a mitochondrial citrate carrier in Arabidopsis thaliana

Includes: Supplementary data

Articles

Biochemical characterization of phospho enol pyruvate carboxykinases from Arabidopsis thaliana

Bruno E. Rojas, Matías D. Hartman, Carlos M. Figueroa, Laura Leaden, Florencio E. Podestá, Alberto A. Iglesias

Biochem J (2019) 476 (20): 2939–2952.

https://doi.org/10.1042/BCJ20190523

Published: 18 October 2019

..., our knowledge on the structural, kinetic and regulatory properties of the enzymes from C 3 species is still limited. In this work, we report the recombinant production and biochemical characterization of two PEPCKs identified in Arabidopsis thaliana : Ath PEPCK1 and Ath PEPCK2. We found that both...

View articletitled, Biochemical characterization of phospho enol pyruvate carboxykinases from Arabidopsis thaliana

Includes: Supplementary data

Articles

Thioredoxin-like2/2-Cys peroxiredoxin redox cascade acts as oxidative activator of glucose-6-phosphate dehydrogenase in chloroplasts

Keisuke Yoshida, Eriko Uchikoshi, Satoshi Hara, Toru Hisabori

Biochem J (2019) 476 (12): 1781–1790.

https://doi.org/10.1042/BCJ20190336

Published: 26 June 2019

... Arabidopsis thaliana as described previously [ 26 ] and used as a template for RT-PCR. The gene fragment encoding the predicted mature protein region of G6PDH1 (At5g35790; Phe 51 –Ala 576 ) was amplified with the following oligonucleotide primers: 5′-CATG CCATGG CGTTCTTCGCCGAGAAACATTC-3′ (NcoI) and 5′-CCG...

View articletitled, Thioredoxin-like2/2-Cys peroxiredoxin redox cascade acts as oxidative activator of glucose-6-phosphate dehydrogenase in chloroplasts

Includes: Supplementary data

Articles

Abscisic acid-determined seed vigour differences do not influence redox regulation during ageing

In Collection

Seeds

Christina Schausberger, Thomas Roach, Wolfgang Stöggl, Erwann Arc, William E. Finch-Savage, Ilse Kranner

Biochem J (2019) 476 (6): 965–974.

https://doi.org/10.1042/BCJ20180903

Published: 22 March 2019

..., cauliflower, broccoli and Brussel sprouts, belong to the same species, Brassica oleracea L., the nearest crop relative to the model plant Arabidopsis thaliana L. According to the Food and Agriculture Organization (FAO), 3.8 million hectares of cabbage, cauliflower and broccoli were harvested in 2016...

View articletitled, Abscisic acid-determined seed vigour differences do not influence redox regulation during ageing

Includes: Supplementary data

Articles

Dynamic hydrolase labelling as a marker for seed quality in Arabidopsis seeds

In Collection

Seeds

Natanael Viñegra de la Torre, Farnusch Kaschani, Markus Kaiser, Renier A. L. van der Hoorn, Wim J. J. Soppe, Johana C. Misas Villamil

Biochem J (2019) 476 (5): 843–857.

https://doi.org/10.1042/BCJ20180911

Published: 12 March 2019

... protein profiling of proteases to evaluate the quality of artificially and naturally aged seeds of Arabidopsis thaliana . Using this approach, we have identified two protease activities with opposite behaviours in aged seeds of Arabidopsis that correlate with the quality status of the seeds. We show...

View articletitled, Dynamic hydrolase labelling as a marker for seed quality in Arabidopsis seeds

Includes: Supplementary data

Articles

The lipid head group is the key element for substrate recognition by the P4 ATPase ALA2: a phosphatidylserine flippase

Lisa Theorin, Kristina Faxén, Danny Mollerup Sørensen, Rebekka Migotti, Gunnar Dittmar, Jürgen Schiller, David L. Daleke, Michael Palmgren, Rosa Laura López-Marqués, Thomas Günther Pomorski

Biochem J (2019) 476 (5): 783–794.

https://doi.org/10.1042/BCJ20180891

Published: 06 March 2019

... in Arabidopsis thaliana , in complex with the ALA-interacting subunit 5 (ALIS5). The ATP hydrolytic activity of the ALA2–ALIS5 complex was stimulated in a highly specific manner by phosphatidylserine. Small changes in the stereochemistry or the functional groups of the phosphatidylserine head group affected...

View articletitled, The lipid head group is the key element for substrate recognition by the P4 ATPase ALA2: a phosphatidylserine flippase

Includes: Supplementary data

Articles

The transmembrane autophagy cargo receptors ATI1 and ATI2 interact with ATG8 through intrinsically disordered regions with distinct biophysical properties

Ida Marie Zobbe Sjøgaard, Simon Bressendorff, Andreas Prestel, Swathi Kausika, Emilie Oksbjerg, Birthe B. Kragelund, Peter Brodersen

Biochem J (2019) 476 (3): 449–465.

https://doi.org/10.1042/BCJ20180748

Published: 05 February 2019

... ( [email protected] ) * These authors contributed equally to this work. Arabidopsis thaliana autophagy intrinsically disordered proteins In addition to these examples of autophagy cargoes and cargo receptors, the Arabidopsis AT G8- I nteracting proteins ATI1 and ATI2 represent two...

View articletitled, The transmembrane autophagy cargo receptors ATI1 and ATI2 interact with ATG8 through intrinsically disordered regions with distinct biophysical properties

Includes: Supplementary data

Articles

Engineering Arabidopsis long-chain acyl-CoA synthetase 9 variants with enhanced enzyme activity

Yang Xu, Kristian Mark P. Caldo, Roman Holic, Elzbieta Mietkiewska, Jocelyn Ozga, Syed Masood Rizvi, Guanqun Chen, Randall J. Weselake

Biochem J (2019) 476 (1): 151–164.

https://doi.org/10.1042/BCJ20180787

Published: 15 January 2019

... the enzyme activity of Arabidopsis thaliana LACS9 (AtLACS9) by introducing random mutations into its cDNA using error-prone PCR. Two AtLACS9 variants containing multiple amino acid residue substitutions were identified with enhanced enzyme activity. To explore the effect of each amino acid residue...

View articletitled, Engineering Arabidopsis long-chain acyl-CoA synthetase 9 variants with enhanced enzyme activity

Includes: Supplementary data

Articles

SUMO chain formation relies on the amino-terminal region of SUMO-conjugating enzyme and has dedicated substrates in plants

Konstantin Tomanov, Lilian Nehlin, Ionida Ziba, Andreas Bachmair

Biochem J (2018) 475 (1): 61–74.

https://doi.org/10.1042/BCJ20170472

Published: 02 January 2018

... by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) . Arabidopsis thaliana AUX/IAA proteins protein modification SUMO SUMO chains In vitro sumoylation of substrate NAF ( Figure 1 ) results in the addition...

View articletitled, SUMO chain formation relies on the amino-terminal region of SUMO-conjugating enzyme and has dedicated substrates in plants

Includes: Supplementary data

Articles

AtSPX1 affects the AtPHR1–DNA-binding equilibrium by binding monomeric AtPHR1 in solution

Wanjun Qi, Iain W. Manfield, Stephen P. Muench, Alison Baker

Biochem J (2017) 474 (21): 3675–3687.

https://doi.org/10.1042/BCJ20170522

Published: 23 October 2017

... in SDS gel-loading buffer, analyzed on SDS–PAGE gels and blotted with anti-GST antibody. Arabidopsis thaliana eukaryotic gene expression myb transcription factor phosphate surface plasmon resonance P1BS probes containing 1× P1BS motif and 2× P1BS motifs were taken from the promoter...

View articletitled, AtSPX1 affects the AtPHR1–DNA-binding equilibrium by binding monomeric AtPHR1 in solution

Includes: Supplementary data

Articles

Arabidopsis fructokinase-like protein associations are regulated by ATP

John W. Riggs, Judy Callis

Biochem J (2017) 474 (11): 1789–1801.

https://doi.org/10.1042/BCJ20161077

Published: 10 May 2017

...John W. Riggs; Judy Callis The Arabidopsis thaliana fructokinase-like proteins FLN1 and FLN2 are required for the differentiation of plastids into photosynthetically competent chloroplasts. However, their specific roles are unknown. FLN1 and FLN2 localize in a multisubunit prokaryotic-type...

View articletitled, Arabidopsis fructokinase-like protein associations are regulated by ATP

Articles

Distinct electron transfer from ferredoxin–thioredoxin reductase to multiple thioredoxin isoforms in chloroplasts

Keisuke Yoshida, Toru Hisabori

Biochem J (2017) 474 (8): 1347–1360.

https://doi.org/10.1042/BCJ20161089

Published: 04 April 2017

..., Arabidopsis thaliana genome sequencing has revealed that as many as five Trx subtypes encoded by a total of 10 nuclear genes are targeted to chloroplasts. Because each Trx isoform seems to have a distinct target selectivity, the electron distribution from FTR to multiple Trxs is thought to be the critical...

View articletitled, Distinct electron transfer from ferredoxin–thioredoxin reductase to multiple thioredoxin isoforms in chloroplasts

Articles

Proteomic and functional analysis of proline dehydrogenase 1 link proline catabolism to mitochondrial electron transport in Arabidopsis thaliana

Cécile Cabassa-Hourton, Peter Schertl, Marianne Bordenave-Jacquemin, Kaouthar Saadallah, Anne Guivarc'h, Sandrine Lebreton, Séverine Planchais, Jennifer Klodmann, Holger Eubel, Emilie Crilat, Delphine Lefebvre-De Vos, Thanos Ghelis, Luc Richard, Chedly Abdelly, Pierre Carol, Hans-Peter Braun, Arnould Savouré

Biochem J (2016) 473 (17): 2623–2634.

https://doi.org/10.1042/BCJ20160314

Published: 30 August 2016

... in the genome of the model plant Arabidopsis thaliana . To gain a better understanding of ProDH1 functions in mitochondria, proteomic analysis was performed. ProDH1 polypeptides were identified in Arabidopsis mitochondria by immunoblotting gels after 2D blue native (BN)-SDS/PAGE, probing them with an anti-ProDH...

View articletitled, Proteomic and functional analysis of proline dehydrogenase 1 link proline catabolism to mitochondrial electron transport in Arabidopsis thaliana

Includes: Supplementary data

Articles

Role of post-translational modifications at the β-subunit ectodomain in complex association with a promiscuous plant P4-ATPase

Sara R. Costa, Magdalena Marek, Kristian B. Axelsen, Lisa Theorin, Thomas G. Pomorski, Rosa L. López-Marqués

Biochem J (2016) 473 (11): 1605–1615.

https://doi.org/10.1042/BCJ20160207

Published: 27 May 2016

... Arabidopsis thaliana Cdc50 ectodomain disulfide bond flippase N-glycosylation The P-type ATPase superfamily comprises a large number of primary transmembrane-spanning transporters that share the common characteristic of forming a phosphorylated intermediate during their catalytic cycle [ 1 ]. P...

View articletitled, Role of post-translational modifications at the β-subunit ectodomain in complex association with a promiscuous plant P4-ATPase

Includes: Supplementary data

Articles

Substrate specificity, kinetic properties and inhibition by fumonisin B 1 of ceramide synthase isoforms from Arabidopsis

Kyle D. Luttgeharm, Edgar B. Cahoon, Jonathan E. Markham

Biochem J (2016) 473 (5): 593–603.

https://doi.org/10.1042/BJ20150824

Published: 24 February 2016

... synthesis of ceramides acting to promote programmed cell death [ 13 – 15 ]. Arabidopsis thaliana ceramide enzyme kinetics plant biochemistry sphingolipid Ceramides are organizing components of sphingolipids in the eukaryotic cell. Three ceramide synthase isoforms are found...

View articletitled, Substrate specificity, kinetic properties and inhibition by fumonisin B 1 of ceramide synthase isoforms from Arabidopsis

Includes: Supplementary data

Articles

Bacterial and plant HAD enzymes catalyse a missing phosphatase step in thiamin diphosphate biosynthesis

Ghulam Hasnain, Sanja Roje, Na Sa, Rémi Zallot, Michael J. Ziemak, Valérie de Crécy-Lagard, Jesse F. Gregory, III, Andrew D. Hanson

Biochem J (2016) 473 (2): 157–166.

https://doi.org/10.1042/BJ20150805

Published: 05 January 2016

... to require an active ThMPase for growth. In vitro assays confirmed that these candidates all preferred ThMP to any of 45 other phosphate ester substrates tested. An Arabidopsis thaliana ThMPase homologue (At4g29530) of unknown function whose expression pattern and compartmentation fit with a role in ThDP...

View articletitled, Bacterial and plant HAD enzymes catalyse a missing phosphatase step in thiamin diphosphate biosynthesis

Includes: Supplementary data

Articles

Conserved phosphorylation sites in the activation loop of the Arabidopsis phytosulfokine receptor PSKR1 differentially affect kinase and receptor activity

Jens Hartmann, Dennis Linke, Christine Bönniger, Andreas Tholey, Margret Sauter

Biochem J (2015) 472 (3): 379–391.

https://doi.org/10.1042/BJ20150147

Published: 27 November 2015

...Jens Hartmann; Dennis Linke; Christine Bönniger; Andreas Tholey; Margret Sauter PSK (phytosulfokine) is a plant peptide hormone perceived by a leucine-rich repeat receptor kinase. Phosphosite mapping of epitope-tagged PSKR1 (phytosulfokine receptor 1) from Arabidopsis thaliana plants identified Ser...

View articletitled, Conserved phosphorylation sites in the activation loop of the Arabidopsis phytosulfokine receptor PSKR1 differentially affect kinase and receptor activity

Includes: Supplementary data

Articles

Biochemical and redox characterization of the mediator complex and its associated transcription factor GeBPL, a GLABROUS1 enhancer binding protein

Jehad Shaikhali, Céline Davoine, Kristoffer Brännström, Nicolas Rouhier, Joakim Bygdell, Stefan Björklund, Gunnar Wingsle

Biochem J (2015) 468 (3): 385–400.

https://doi.org/10.1042/BJ20150132

Published: 15 June 2015

... be crucial to modulate the activity of transcription factors (TFs) in response to environmental signals/stress. Arabidopsis thaliana CryR2 deoxyribonucleic acid (DNA)-binding GLABROUS1 enhancer-binding protein (GeBP) mediator redox Mediator was originally identified in Saccharomyces...

View articletitled, Biochemical and redox characterization of the mediator complex and its associated transcription factor GeBPL, a GLABROUS1 enhancer binding protein

Includes: Supplementary data

Articles

A stable RNA G-quadruplex within the 5′-UTR of Arabidopsis thaliana ATR mRNA inhibits translation

Chun Kit Kwok, Yiliang Ding, Saima Shahid, Sarah M. Assmann, Philip C. Bevilacqua

Biochem J (2015) 467 (1): 91–102.

https://doi.org/10.1042/BJ20141063

Published: 20 March 2015

.... This GQS is located within the 5′-UTR of the ATAXIA TELANGIECTASIA-MUTATED AND RAD3-RELATED ( ATR ) mRNA of Arabidopsis thaliana (mouse-ear cress). We show that this GQS is highly stable and is thermodynamically favoured over a competing hairpin structure in the 5′-UTR at physiological K + and Mg 2...

View articletitled, A stable RNA G-quadruplex within the 5′-UTR of Arabidopsis thaliana ATR mRNA inhibits translation

Includes: Supplementary data

Articles

Trehalose-6-phosphate synthase 1 is not the only active TPS in Arabidopsis thaliana

Ines Delorge, Carlos M. Figueroa, Regina Feil, John E. Lunn, Patrick Van Dijck

Biochem J (2015) 466 (2): 283–290.

https://doi.org/10.1042/BJ20141322

Published: 20 February 2015

... ). 23 10 2014 1 12 2014 12 12 2014 12 12 2014 © The Authors Journal compilation © 2015 Biochemical Society 2015 We show that Arabidopsis thaliana has at least three catalytically active trehalose 6-phosphate synthase isoforms, not one as previously thought, implying...

View articletitled, Trehalose-6-phosphate synthase 1 is not the only active TPS in Arabidopsis thaliana

Articles

A directed-overflow and damage-control N -glycosidase in riboflavin biosynthesis

Océane Frelin, Lili Huang, Ghulam Hasnain, James G. Jeffryes, Michael J. Ziemak, James R. Rocca, Bing Wang, Jennifer Rice, Sanja Roje, Svetlana N. Yurgel, Jesse F. Gregory, III, Arthur S. Edison, Christopher S. Henry, Valérie de Crécy-Lagard, Andrew D. Hanson

Biochem J (2015) 466 (1): 137–145.

https://doi.org/10.1042/BJ20141237

Published: 06 February 2015

... in Arabidopsis thaliana and Escherichia coli helps maintain flavin levels. COG3236 proteins thus illustrate two emerging principles in chemical biology: directed overflow metabolism, in which excess flux is diverted out of a pathway, and the pre-emption of damage from reactive metabolites. Subsequent steps...

View articletitled, A directed-overflow and damage-control N -glycosidase in riboflavin biosynthesis

Includes: Supplementary data

Articles

Plant cyclopropylsterol-cycloisomerase: key amino acids affecting activity and substrate specificity

Alain Rahier, Francis Karst

Biochem J (2014) 459 (2): 289–299.

https://doi.org/10.1042/BJ20131239

Published: 28 March 2014

... the Arabidopsis thaliana cpi gene were screened for inactive CPI mutant enzymes on the basis of their ability to genetically complement a Saccharomyces cerevisiae erg7 (defective in oxidosqualene cyclase) ergosterol auxotroph grown in the presence of exogenous cycloeucalenol, and led to the identification of four...

View articletitled, Plant cyclopropylsterol-cycloisomerase: key amino acids affecting activity and substrate specificity

Includes: Supplementary data

Articles

Analysis of Arabidopsis thioredoxin-h isotypes identifies discrete domains that confer specific structural and functional properties

Young Jun Jung, Yong Hun Chi, Ho Byoung Chae, Mi Rim Shin, Eun Seon Lee, Joon-Yung Cha, Seol Ki Paeng, Yuno Lee, Jin Ho Park, Woe Yeon Kim, Chang Ho Kang, Kyun Oh Lee, Keun Woo Lee, Dae-Jin Yun, Sang Yeol Lee

Biochem J (2013) 456 (1): 13–24.

https://doi.org/10.1042/BJ20130618

Published: 24 October 2013

...Young Jun Jung; Yong Hun Chi; Ho Byoung Chae; Mi Rim Shin; Eun Seon Lee; Joon-Yung Cha; Seol Ki Paeng; Yuno Lee; Jin Ho Park; Woe Yeon Kim; Chang Ho Kang; Kyun Oh Lee; Keun Woo Lee; Dae-Jin Yun; Sang Yeol Lee Multiple isoforms of Arabidopsis thaliana h-type thioredoxins (AtTrx-hs) have distinct...

View articletitled, Analysis of Arabidopsis thioredoxin-h isotypes identifies discrete domains that confer specific structural and functional properties

Includes: Supplementary data

Articles

The folding capacity of the mature domain of the dual-targeted plant tRNA nucleotidyltransferase influences organelle selection

Matthew Leibovitch, Daniela Bublak, Pamela J. Hanic-Joyce, Bodo Tillmann, Nadine Flinner, Daniel Amsel, Klaus-Dieter Scharf, Oliver Mirus, Paul B. M. Joyce, Enrico Schleiff

Biochem J (2013) 453 (3): 401–412.

https://doi.org/10.1042/BJ20121577

Published: 12 July 2013

... of four regions, the N-terminal head domain, the neck domain, the body region and the C-terminal tail ( Figure 1 A) [ 5 ]. © The Authors Journal compilation © 2013 Biochemical Society 2013 Arabidopsis thaliana in vivo protein translocation protein stability protein targeting tRNA...

View articletitled, The folding capacity of the mature domain of the dual-targeted plant tRNA nucleotidyltransferase influences organelle selection

Includes: Supplementary data

Articles

Regulation of the V-type ATPase by redox modulation

Thorsten Seidel, Stefan Scholl, Melanie Krebs, Florian Rienmüller, Irene Marten, Rainer Hedrich, Miriam Hanitzsch, Patricia Janetzki, Karl-Josef Dietz, Karin Schumacher

Biochem J (2012) 448 (2): 243–251.

https://doi.org/10.1042/BJ20120976

Published: 07 November 2012

... (email [email protected] or [email protected] ). 15 6 2012 28 8 2012 4 9 2012 4 9 2012 © The Authors Journal compilation © 2012 Biochemical Society 2012 Arabidopsis thaliana glutathione nitrosoglutathione (GSNO) redox...

View articletitled, Regulation of the V-type ATPase by redox modulation

Includes: Supplementary data

Articles

Characterization of Arabidopsis calcium-dependent protein kinases: activated or not by calcium?

Marie Boudsocq, Marie-Jo Droillard, Leslie Regad, Christiane Laurière

Biochem J (2012) 447 (2): 291–299.

https://doi.org/10.1042/BJ20112072

Published: 26 September 2012

... All experiments were performed with Arabidopsis thaliana ecotype Columbia (Col0) WT (wild-type) or CPK-overexpressing transgenic lines. For seedling assays, seeds were sterilized and grown in 0.5×Murashige and Skoog liquid medium (Sigma) containing 0.5% sucrose for 9 days at 21°C with a 16 h...

View articletitled, Characterization of Arabidopsis calcium-dependent protein kinases: activated or not by calcium?

Includes: Supplementary data

Articles

Characterization of the evolutionarily conserved iron–sulfur cluster of sirohydrochlorin ferrochelatase from Arabidopsis thaliana

Kaushik Saha, Michael E. Webb, Stephen E. J. Rigby, Helen K. Leech, Martin J. Warren, Alison G. Smith

Biochem J (2012) 444 (2): 227–237.

https://doi.org/10.1042/BJ20111993

Published: 11 May 2012

... by methylation, oxidation and ferrochelation reactions. In Arabidopsis thaliana , the ferrochelation step is catalysed by sirohydrochlorin ferrochelatase (SirB), which, unlike its counterparts in bacteria, contains an [Fe–S] cluster. We determined the cluster to be a [4Fe–4S] type, which quickly oxidizes...

View articletitled, Characterization of the evolutionarily conserved iron–sulfur cluster of sirohydrochlorin ferrochelatase from Arabidopsis thaliana

Includes: Supplementary data

Articles

Crystal structure and functional characterization of a glucosamine-6-phosphate N-acetyltransferase from Arabidopsis thaliana

Heike Riegler, Thomas Herter, Irina Grishkovskaya, Anja Lude, Malgorzata Ryngajllo, Marie E. Bolger, Bernd Essigmann, Björn Usadel

Biochem J (2012) 443 (2): 427–437.

https://doi.org/10.1042/BJ20112071

Published: 27 March 2012

... are above or to the right of a reaction arrow. Prokaryotic enzymes are indicated below or left of a reaction arrow. Arabidopsis thaliana glucosamine-6-phosphate N-acetyltransferase (GNA) glycosylation N -acetylglucosamine biosynthesis nucleotide sugar GlcNAc ( N -acetylglucosamine...

View articletitled, Crystal structure and functional characterization of a glucosamine-6-phosphate N-acetyltransferase from Arabidopsis thaliana

Includes: Supplementary data

Articles

The pH sensor of the plant K + -uptake channel KAT1 is built from a sensory cloud rather than from single key amino acids

Wendy González, Janin Riedelsberger, Samuel E. Morales-Navarro, Julio Caballero, Jans H. Alzate-Morales, Fernando D. González-Nilo, Ingo Dreyer

Biochem J (2012) 442 (1): 57–63.

https://doi.org/10.1042/BJ20111498

Published: 27 January 2012

... (approximately 2–2.5 pH units) is perceived by voltage-gated inward potassium channels (K in ) that then can open their pores with lower energy cost. The sensory units for extracellular pH in stomatal K in channels are proposed to be histidines exposed to the apoplasm. However, in the Arabidopsis thaliana...

View articletitled, The pH sensor of the plant K + -uptake channel KAT1 is built from a sensory cloud rather than from single key amino acids

Articles

Identification of persulfide-binding and disulfide-forming cysteine residues in the NifS-like domain of the molybdenum cofactor sulfurase ABA3 by cysteine-scanning mutagenesis

Markus Lehrke, Steffen Rump, Torsten Heidenreich, Josef Wissing, Ralf R. Mendel, Florian Bittner

Biochem J (2012) 441 (3): 823–839.

https://doi.org/10.1042/BJ20111170

Published: 16 January 2012

...Markus Lehrke; Steffen Rump; Torsten Heidenreich; Josef Wissing; Ralf R. Mendel; Florian Bittner The Moco (molybdenum cofactor) sulfurase ABA3 from Arabidopsis thaliana catalyses the sulfuration of the Moco of aldehyde oxidase and xanthine oxidoreductase, which represents the final activation step...

View articletitled, Identification of persulfide-binding and disulfide-forming cysteine residues in the NifS-like domain of the molybdenum cofactor sulfurase ABA3 by cysteine-scanning mutagenesis

Includes: Supplementary data

Articles

Inter- and intra-molecular interactions of Arabidopsis thaliana DELLA protein RGL1

David J. Sheerin, Jeremy Buchanan, Chris Kirk, Dawn Harvey, Xiaolin Sun, Julian Spagnuolo, Sheng Li, Tong Liu, Virgil A. Woods, Toshi Foster, William T. Jones, Jasna Rakonjac

Biochem J (2011) 435 (3): 629–639.

https://doi.org/10.1042/BJ20101941

Published: 13 April 2011

... of signalling between the N- and C-terminal domains of DELLA proteins is unresolved. In the present study, we used in vivo and in vitro approaches to characterize di- and tri-partite interactions of the DELLA protein RGL1 (REPRESSOR OF GA1-3-LIKE 1) of Arabidopsis thaliana with the gibberellin receptor GID1A...

View articletitled, Inter- and intra-molecular interactions of Arabidopsis thaliana DELLA protein RGL1

Includes: Supplementary data

Articles

A new principle of oligomerization of plant DEG7 protease based on interactions of degenerated protease domains

Holger Schuhmann, Ulrike Mogg, Iwona Adamska

Biochem J (2011) 435 (1): 167–174.

https://doi.org/10.1042/BJ20101613

Published: 15 March 2011

... unrestricted non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited. Arabidopsis thaliana complex formation degenerated protease domain DEG7 serine protease taxonomic distribution Deg/HtrA (for deg radation of periplasmic proteins/ h...

View articletitled, A new principle of oligomerization of plant DEG7 protease based on interactions of degenerated protease domains

Includes: Supplementary data

Articles

The Arabidopsis class I TCP transcription factor AtTCP11 is a developmental regulator with distinct DNA-binding properties due to the presence of a threonine residue at position 15 of the TCP domain

Ivana L. Viola, Nora G. Uberti Manassero, Rodrigo Ripoll, Daniel H. Gonzalez

Biochem J (2011) 435 (1): 143–155.

https://doi.org/10.1042/BJ20101019

Published: 15 March 2011

... correspondence should be addressed (email [email protected] ). 8 7 2010 23 12 2010 17 1 2011 17 1 2011 © The Authors Journal compilation © 2011 Biochemical Society 2011 Arabidopsis thaliana DNA-binding specificity EAR domain plant development systematic...

View articletitled, The Arabidopsis class I TCP transcription factor AtTCP11 is a developmental regulator with distinct DNA-binding properties due to the presence of a threonine residue at position 15 of the TCP domain

Includes: Supplementary data

Articles

NAD-malic enzymes of Arabidopsis thaliana display distinct kinetic mechanisms that support differences in physiological control

Marcos A. Tronconi, Mariel C. Gerrard Wheeler, Verónica G. Maurino, María F. Drincovich, Carlos S. Andreo

Biochem J (2010) 430 (2): 295–303.

https://doi.org/10.1042/BJ20100497

Published: 13 August 2010

...Marcos A. Tronconi; Mariel C. Gerrard Wheeler; Verónica G. Maurino; María F. Drincovich; Carlos S. Andreo The Arabidopsis thaliana genome contains two genes encoding NAD-MEs [NAD-dependent malic enzymes; NAD-ME1 (TAIR accession number At4G13560) and NAD-ME2 (TAIR accession number At4G00570...

View articletitled, NAD-malic enzymes of Arabidopsis thaliana display distinct kinetic mechanisms that support differences in physiological control

Includes: Supplementary data

Articles

The Arabidopsis thaliana NAC transcription factor family: structure–function relationships and determinants of ANAC019 stress signalling

Michael K. Jensen, Trine Kjaersgaard, Michael M. Nielsen, Pernille Galberg, Klaus Petersen, Charlotte O'Shea, Karen Skriver

Biochem J (2010) 426 (2): 183–196.

https://doi.org/10.1042/BJ20091234

Published: 09 February 2010

...), ATAF, CUC (cup-shaped cotyledon)] proteins comprise one of the largest plant TF families. They are key regulators of stress perception and developmental programmes, and most share an N-terminal NAC domain. On the basis of analyses of gene expression data and the phylogeny of Arabidopsis thaliana NAC...

View articletitled, The Arabidopsis thaliana NAC transcription factor family: structure–function relationships and determinants of ANAC019 stress signalling

Includes: Supplementary data

Articles

Characterization of the haem oxygenase protein family in Arabidopsis thaliana reveals a diversity of functions

Bjoern Gisk, Yukiko Yasui, Takayuki Kohchi, Nicole Frankenberg-Dinkel

Biochem J (2010) 425 (2): 425–434.

https://doi.org/10.1042/BJ20090775

Published: 23 December 2009

... essential for proper photomorphogenesis. Arabidopsis thaliana contains one major biochemically characterized HO (HY1) and three additional putative HOs (HO2, HO3 and HO4). All four proteins are encoded in the nucleus but contain chloroplast translocation sequences at their N-termini. The transit peptides...

View articletitled, Characterization of the haem oxygenase protein family in Arabidopsis thaliana reveals a diversity of functions

Articles

Chloroplast HCF101 is a scaffold protein for [4Fe-4S] cluster assembly

Serena Schwenkert, Daili J. A. Netz, Jeverson Frazzon, Antonio J. Pierik, Eckhard Bill, Jeferson Gross, Roland Lill, Jörg Meurer

Biochem J (2010) 425 (1): 207–218.

https://doi.org/10.1042/BJ20091290

Published: 14 December 2009

... is properly cited. [4Fe-4S]-cluster-containing P-loop NTPase (FSC-NTPase) Arabidopsis thaliana chloroplast high chlorophyll fluorescence 101 (HCF101) iron-sulfur cluster assembly scaffold protein Fe/S clusters (iron-sulfur clusters) are ancient cofactors of proteins required for various...

View articletitled, Chloroplast HCF101 is a scaffold protein for [4Fe-4S] cluster assembly

Includes: Supplementary data

Articles

Eukaryotic GCP1 is a conserved mitochondrial protein required for progression of embryo development beyond the globular stage in Arabidopsis thaliana

Kirsten Haussuehl, Pitter F. Huesgen, Marc Meier, Patrick Dessi, Elżbieta Glaser, Jerzy Adamski, Iwona Adamska

Biochem J (2009) 423 (3): 333–341.

https://doi.org/10.1042/BJ20091023

Published: 12 October 2009

... study on GCP1-type proteins from eukaryotic organisms we demonstrated that GCP1 is a mitochondrial protein in Homo sapiens [called here GCP1/OSGEPL1 ( O -sialoglycoprotein endopeptidase)] and Arabidopsis thaliana , which is located/anchored to the mitochondrial inner membrane. Analysis of mRNA...

View articletitled, Eukaryotic GCP1 is a conserved mitochondrial protein required for progression of embryo development beyond the globular stage in Arabidopsis thaliana

Includes: Supplementary data

Articles

Single-Myb-histone proteins from Arabidopsis thaliana : a quantitative study of telomere-binding specificity and kinetics

Ctirad Hofr, Pavla Šultesová, Michal Zimmermann, Iva Mozgová, Petra Procházková Schrumpfová, Michaela Wimmerová, Jiří Fajkus

Biochem J (2009) 419 (1): 221–230.

https://doi.org/10.1042/BJ20082195

Published: 13 March 2009

...Ctirad Hofr; Pavla Šultesová; Michal Zimmermann; Iva Mozgová; Petra Procházková Schrumpfová; Michaela Wimmerová; Jiří Fajkus Proteins that bind telomeric DNA modulate the structure of chromosome ends and control telomere function and maintenance. It has been shown that AtTRB ( Arabidopsis thaliana...

View articletitled, Single-Myb-histone proteins from Arabidopsis thaliana : a quantitative study of telomere-binding specificity and kinetics

Includes: Supplementary data

Articles

Identification of new protein substrates for the chloroplast ATP-dependent Clp protease supports its constitutive role in Arabidopsis

Tara M. Stanne, Lars L. E. Sjögren, Shai Koussevitzky, Adrian K. Clarke

Biochem J (2009) 417 (1): 257–269.

https://doi.org/10.1042/BJ20081146

Published: 12 December 2008

... of one of these subunits in Arabidopsis thaliana , ClpR1. Loss of ClpR1 caused a slow-growth phenotype, with chlorotic leaves during early development that later partially recovered upon maturity. Analysis of the Clp proteolytic core in the clpR1 mutant ( clpR1-1 ) revealed approx. 10% of the wild-type...

View articletitled, Identification of new protein substrates for the chloroplast ATP-dependent Clp protease supports its constitutive role in Arabidopsis

Includes: Supplementary data

Articles

Salt-stress-induced association of phosphatidylinositol 4,5-bisphosphate with clathrin-coated vesicles in plants

Sabine König, Till Ischebeck, Jennifer Lerche, Irene Stenzel, Ingo Heilmann

Biochem J (2008) 415 (3): 387–399.

https://doi.org/10.1042/BJ20081306

Published: 15 October 2008

... [email protected] ). 26 6 2008 5 8 2008 22 8 2008 22 8 2008 © The Authors Journal compilation © 2008 Biochemical Society 2008 All experiments were performed with Arabidopsis thaliana ecotype Columbia 0 (col-0). Plants were grown on soil under exposure to 140 μmol...

View articletitled, Salt-stress-induced association of phosphatidylinositol 4,5-bisphosphate with clathrin-coated vesicles in plants

Includes: Supplementary data

Articles

A novel protein phosphatase indirectly regulates phytochrome-interacting factor 3 via phytochrome

Bong-Kwan Phee, Jeong-Il Kim, Dong Ho Shin, Jihye Yoo, Kyoung-Jin Park, Yun-Jeong Han, Yong-Kook Kwon, Man-Ho Cho, Jong-Seong Jeon, Seong Hee Bhoo, Tae-Ryong Hahn

Biochem J (2008) 415 (2): 247–255.

https://doi.org/10.1042/BJ20071555

Published: 25 September 2008

... in the nucleus. 1 These authors contributed equally to the present study. Arabidopsis thaliana dephosphorylation phosphatase phytochrome p hytochrome- a ssociated p rotein p hosphatase type 2C (PAPP2C) phytochrome-interacting factor 3 (PIF3) Plant light signal transduction...

View articletitled, A novel protein phosphatase indirectly regulates phytochrome-interacting factor 3 via phytochrome

Includes: Supplementary data

Articles

Diverse roles for chloroplast stromal and thylakoid-bound ascorbate peroxidases in plant stress responses

Saijaliisa Kangasjärvi, Anna Lepistö, Kati Hännikäinen, Mirva Piippo, Eeva-Maria Luomala, Eva-Mari Aro, Eevi Rintamäki

Biochem J (2008) 412 (2): 275–285.

https://doi.org/10.1042/BJ20080030

Published: 14 May 2008

... an important role in the antioxidant system of plants. In the present study we addressed the significance of chloroplast APXs in stress tolerance and signalling in Arabidopsis thaliana . To this end, T-DNA (transfer DNA) insertion mutants tapx , sapx and tapx sapx , lacking the tAPX (thylakoid-bound APX), sAPX...

View articletitled, Diverse roles for chloroplast stromal and thylakoid-bound ascorbate peroxidases in plant stress responses

Includes: Supplementary data

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