...Min-Kyu Kwak; Han-Bong Ryu; Sung-Hyun Song; Jin-Won Lee; Sa-Ouk Kang YlaD, a membrane-anchored anti-sigma (σ) factor of Bacillus subtilis , contains a HX 3 CXXC motif that functions as a redox-sensing domain and belongs to one of the zinc (Zn)-co-ordinated anti-σ factor families. Despite previously...

... separated by a spacer of 17±1 bp for optimal activity. However, how the optimal promoter spacing is sensed by the primary σ remains unclear. In the present study, we examined this issue using a transcriptionally active Bacillus subtilis N-terminally truncated σ A (SND100-σ A ). The results of the present...

...Yang Du; Wei-Wei Shi; Yong-Xing He; Yi-Hu Yang; Cong-Zhao Zhou; Yuxing Chen The compatible solute ABC (ATP-binding cassette) transporters are indispensable for acquiring a variety of compatible solutes under osmotic stress in Bacillus subtilis . The substrate-binding protein OpuCC (Opu...

...Karin E. van Straaten; Hongyan Zheng; David R. J. Palmer; David A. R. Sanders Inositol dehydrogenase from Bacillus subtilis (BsIDH) is a NAD + -dependent enzyme that catalyses the oxidation of the axial hydroxy group of myo -inositol to form scyllo -inosose. We have determined the crystal...

...Chloe Singleton; Stephen Hearnshaw; Liang Zhou; Nick E. Le Brun; Andrew M. Hemmings Multinuclear Cu(I) clusters are common in nature, but little is known about their formation or transfer between proteins. CopZ and CopA from Bacillus subtilis , which are involved in a copper-efflux pathway, both...

... arabinofuranosyl substituents and xylopyranosyl backbone residues of arabinoxylan. Bacillus subtilis was recently shown to produce an AXH that cleaves arabinose units from O-2- or O-3-mono-substituted xylose residues: Bs AXH-m2,3 ( B. subtilis AXH-m2,3). Crystallographic analysis reveals a two-domain structure...

...Liang Zhou; Chloe Singleton; Nick E. Le Brun CopZ, an Atx1-like copper chaperone from the bacterium Bacillus subtilis , functions as part of a complex cellular machinery for Cu(I) trafficking and detoxification, in which it interacts specifically with the transmembrane Cu(I)-transporter CopA. Here...

...Chloe Singleton; Lucia Banci; Simone Ciofi-Baffoni; Leonardo Tenori; Margaret A. Kihlken; Ruth Boetzel; Nick E. Le Brun CopA, a P-type ATPase from Bacillus subtilis , plays a major role in the resistance of the cell to copper by effecting the export of the metal across the cytoplasmic membrane...

... of a relatively inert C–C bond [ 1 , 2 ]. The enzyme, coded by the oxdC gene in the bacterium Bacillus subtilis , is thought to be involved in the response to acid pH stress [ 3 ] and a recent study has reported that it is unexpectedly targeted to the cell wall [ 4 ]. It requires Mn(II) and, uniquely, dioxygen...

...Jing Wu; Galina Ya. Sheflyan; Ronald W. Woodard The mono/bifunctional and metallo/non-metallo properties of Bacillus subtilis DAHPS (3-deoxy- D - arabino -heptulosonate 7-phosphate synthase) have been controversial for several decades. The present study investigated the DAHPSs from both the B...

.... The primers used for the site-directed mutagenesis (which introduced the W190L mutation) were W190L-FW, 5′-ACTCAGAAGAAAAATTGTTTGACAAAAT-3′, and W190L-BW, 5′-GCAATTTTGTCAAACAATTTTTCTTCTG-3′. The Biochemical Society, London 2004 Bacillus subtilis differential gene expression fluorescence...

... of the purified enzyme generated patterns consistent with the amino acid sequence coded for by the cloned cDNA. A BLAST search of the SwissProt database revealed that A. mellea nuclease shared significant amino acid similarity with two nucleases from Bacillus subtilis , suggesting that the three might constitute...