... for prion propagation in transmissible spongiform encephalopathies. Pentapeptides PrP-(92–96) and PrP-(107–111) represent the minimum motif for Cu 2+ binding to the PrP-(90–126) fragment. Consequently, we were surprised that the appearance of the visible CD spectra for two fragments of PrP, residues 90–126...

.... Native polyacrylamide gel electrophoresis showed, that mutant p.Y143C subunits are able to form the tetrameric complex as is the wild-type enzyme. CD analysis showed that the p.Y143C mutation renders the recombinant protein thermosensitive, with an unfolding temperature significantly reduced by 7 °C...

... milligram quantities of the isolated C-terminus of ClC-5 (CIC-5 Ct). CD studies show that the protein is compact, with predominantly α-helical structure. We determined, using radiolabelled ATP, that this nucleotide binds the folded protein with low affinity, in the millimolar range...

... synthesized and their interactions with membranes studied by (i) CD, (ii) a filtration-binding assay and (iii) a fluorescent molecule leakage assay. Model membranes that varied in lipid compositions and radius of curvature were utilized to determine the compositional and structural requirements for optimal...

...-dodecyl-β- d -maltoside-solubilized UCP1–His 6 retained its PN (purine nucleotide)-binding capacity. The far-UV CD spectrum of the functional protein clearly indicated the predominance of α-helices in the UCP1 secondary structure. The UCP1 secondary structure exhibited an α-helical degree of approx. 68...

..., and the haemolytic effects occurred at concentrations 10- to 100-fold higher than those required for bacterial cell lysis. For insight into the mechanism of action of SC4 peptide-amphiphiles, CD, NMR and fluorescence spectroscopy studies were performed in micelle and liposome models of eukaryotic and bacterial cell...

...Irene KARANTZENI; Carmen RUIZ; Chin-Chi LIU; Vince J. LiCATA Thermal denaturations of the type 1 DNA polymerases from Thermus aquaticus ( Taq polymerase) and Escherichia coli (Pol 1) have been examined using differential scanning calorimetry and CD spectroscopy. The full-length proteins are single...

...Monika OBERER; Klaus ZANGGER; Stefan PRYTULLA; Walter KELLER NMR and CD spectroscopy have been used to characterize, both structurally and dynamically, the 82-amino-acid ParD protein of the post-segregational killing module of the broad-host-range plasmid RP4/RK2. ParD occurs as a dimer in solution...

... alignments are the same in all variant tetramers. CD data for all variants indicate mostly β-sheet character in dimers and random coil character in monomers. Only for the V22I variant is the β-sheet fold stabilized in the monomer state. Pulse-field gradient NMR-derived diffusion coefficients, measured...

... properties at low pH and a protease-resistant N-terminal domain at pH4 and pH8 comprising residues 63–193. To explore the mechanism of action of StAR we investigated the structural properties of the bacterially expressed N-terminal domain (63–193 StAR) using CD, limited proteolysis and NMR. Far- and near-UV...

... and is not haemolytic below 10 -4 M. For all SC peptides, CD and NMR data indicate the presence of both 3 10 - and α-helix. For SC-4, nuclear-Overhauser-effect-based computational modelling yields an amphipathic helix with K1, K4, R5, and K8 arrayed on the same face (K is lysine, R is arginine). Activity differences...

..., and 46 μ M and 142 μ M for adenylate kinase. For ATPase activity, genistein reduced V max by 29% and V max / K m by 77%. Additional evidence for complex-formation between genistein and MBP-NBF-2 was obtained by the detection of genistein-dependent alterations in the CD spectrum of MBP-NBF-2 that were...