... are much higher in embryonic and neonate mouse brain compared with the adult tissue. We also demonstrate, by comparing wild-type and Cd38 −/− tissues, that brain cADPR content is independent of the presence of CD38 (the best characterized mammalian ADP-ribosyl cyclase) not only in adult but also...

...), a major degradation product of NAD + , also resulted in a rise in [Ca 2+ ] i . Selective inhibition of CD38, an NAD-glycohydrolase that generates free ADPR from NAD + , does not abolish the effect of NAD + , excluding the possibility that NAD + might act via ADPR. The NAD + -induced Ca 2+ response...

... of NAADP was highly sensitive to nicotinamide inhibition, this was not via a competition with the nicotinic-acid-binding site. In contrast with the ecto-ADP-ribosyl cyclase (CD38), the cyclization and base-exchange reaction of the skeletal muscle isoform was inhibited by Cu 2+ and Zn 2+ , while other...

.... 27 5 2003 28 5 2003 28 5 2003 8 7 2003 The Biochemical Society, London ©2003 2003 Ca 2+ mobilization cADP-ribose CD38 inositol phospholipids insulin nicotinic acid—adenine dinucleotide phosphate (NAADP) 1 To whom correspondence should be addressed (e...

...Claire CENI; Nathalie POCHON; Virginie BRUN; Hélène MULLER-STEFFNER; Annie ANDRIEUX; Didier GRUNWALD; Francis SCHUBER; Michel DE WAARD; Frances LUND; Michel VILLAZ; Marie-Jo MOUTIN CD38 is a transmembrane glycoprotein that is expressed in many tissues throughout the body. In addition to its major...

...+ signalling CD38 NAD + NADH Abbreviations used: cADPR, cADP-ribose; NAADP, nicotinic acid adenine dinucleotide phosphate; NHD, nicotinamide hypoxanthine dinucleotide; RIA, radioimmunoassay. Biochem. J. (2002) 361, 379 384 (Printed in Great Britain) 379 A novel cycling assay for cellular cADP...