... in Caenorhabditis elegans that is highly conserved across nematode species. We show that RML-1 activates glucose 1-phosphate (Glc-1-P) in the presence of either dTTP or UTP to yield dTDP-glucose or UDP-glucose, respectively. RML-2 is a dTDP-glucose 4,6-dehydratase, converting dTDP-glucose into dTDP-4-keto-6...

...Hyoe-Jin Joo; Saeram Park; Kwang-Youl Kim; Mun-Young Kim; Heekyeong Kim; Donha Park; Young-Ki Paik The nematode worm Caenorhabditis elegans survives by adapting to environmental stresses such as temperature extremes by increasing the concentrations of ascaroside pheromones, termed ascarosides...

...Hsiang Yu; Huey-Jen Lai; Tai-Wei Lin; Chang-Shi Chen; Szecheng J. Lo Three waves of apoptosis shape the development of Caenorhabditis elegans . Although the exact roles of the three DNase II genes ( nuc-1 , crn-6 and crn-7 ), which are known to mediate degradation of apoptotic DNA, in the embryonic...

... and remains one of the most important anthelmintic drugs for medical and veterinary use. We have characterized how Caenorhabditis elegans metabolizes and excretes TBZ. We have shown that TBZ directly binds to the nuclear hormone receptor (NHR)-176 and that this receptor is required for the induction by TBZ...

...Chiung-Wen Hu; Jian-Lian Chen; Yu-Wen Hsu; Cheng-Chieh Yen; Mu-Rong Chao From 1986 to the present, the popular research model organism Caenorhabditis elegans has been thought to completely lack DNA methylation and seems to have lost DNA methylation enzymes from its genomes. In the present study, we...

... in a transactivation cell-based luciferase assay and rescued the Mig phenotype of daf-9 ( rh50 ) Caenorhabditis elegans mutants. In the present paper, to investigate further the relationship between the structure of the steroid side chain and DAF-12 activity, we evaluated the in vitro and in vivo activity of Δ 4 -DA...

...Julia Keller; Alexandra Ellieva; Dengke K. Ma; Jingjuan Ju; Erik Nehk; Anne Konkel; John R. Falck; Wolf-Hagen Schunck; Ralph Menzel A specific behavioural response of Caenorhabditis elegans , the rapid increase of locomotion in response to anoxia/reoxygenation called the O2-ON response, has been...

...Wen-ming Huang; Zhao-yu Li; Yan-jun Xu; Wei Wang; Mao-ge Zhou; Peng Zhang; Ping-sheng Liu; Tao Xu; Zheng-xing Wu Lysosomes act as terminal degradation organelles to hydrolyse macromolecules derived from both the extracellular space and the cytoplasm. In Caenorhabditis elegans fasting induces...

...Ying Feng; Barnabas G. Williams; Françoise Koumanov; Adrian J. Wolstenholme; Geoffrey D. Holman Caenorhabditis elegans is widely used as a model for investigation of the relationships between aging, nutrient restriction and signalling via the DAF-2 (abnormal dauer formation 2) receptor for insulin...

... between yeast and higher eukaryotes. Very little is known about essential autophagy components specific to higher eukaryotes. Recent studies have shown that a variety of protein aggregates are selectively removed by autophagy (a process termed aggrephagy) during Caenorhabditis elegans embryogenesis...

...Takahiro Tanji; Kenji Nishikori; Hirohisa Shiraishi; Masatomo Maeda; Ayako Ohashi-Kobayashi Caenorhabditis elegans HAF-4 and HAF-9 are half ABC (ATP-binding-cassette) transporters that are highly homologous to the human lysosomal peptide transporter TAPL [TAP (transporter associated with antigen...

... describe a novel structural arrangement of the CBS enzyme encoded by the cbs-1 gene of the nematode Caenorhabditis elegans . The CBS-1 protein contains a unique tandem repeat of two evolutionarily conserved catalytic regions in a single polypeptide chain. These repeats include a catalytically active C...

... eukaryotic species, indicating that it performs a basal cellular function. However, differential tissue expression and the existence of several ACBP paralogues in many eukaryotic species indicate that these proteins serve distinct functions. The nematode Caenorhabditis elegans expresses seven ACBPs: four...

...Mandy Kosel; Waltraud Wild; Alexandra Bell; Michael Rothe; Carsten Lindschau; Christian E. W. Steinberg; Wolf-Hagen Schunck; Ralph Menzel Caenorhabditis elegans harbours several CYP (cytochrome P450) genes that are homologous with mammalian CYP isoforms important to the production...

... that novel players remain to be identified. A search for mutants in Caenorhabditis elegans with a Hid ( h igh-temperature- i nduced d auer formation) phenotype, identified mutations in the hid-1 gene [ 6 ]. The hid-1 gene encodes a highly conserved putative protein (HID-1) with homologues...

...Ana C. Calvo; Angel L. Pey; Antonio Miranda-Vizuete; Anne P. Døskeland; Aurora Martinez TH (tyrosine hydroxylase) is the rate-limiting enzyme in the synthesis of catecholamines. The cat-2 gene of the nematode Caenorhabditis elegans is expressed in mechanosensory dopaminergic neurons and has been...

...Hyoe-Jin Joo; Yong-Hyeon Yim; Pan-Young Jeong; You-Xun Jin; Jeong-Eui Lee; Heekyeong Kim; Seul-Ki Jeong; David J. Chitwood; Young-Ki Paik Caenorhabditis elegans excretes a dauer pheromone or daumone composed of ascarylose and a fatty acid side chain, the perception of which enables worms to enter...

... the identification of biochemical targets not found in host organisms. Recent studies suggest that Caenorhabditis elegans synthesizes phosphocholine through the action of PEAMT ( S -adenosyl- L -methionine:phosphoethanolamine N -methyltransferases) that convert phosphoethanolamine into phosphocholine. Here, we...

... mitochondrial translation system employs two different EF-Tu factors named EF-Tu1 and EF-Tu2. Our previous study showed that nematode Caenorhabditis elegans EF-Tu1 binds specifically to T-armless tRNA. C. elegans EF-Tu1 has a 57-amino acid C-terminal extension that is absent from canonical EF-Tu, and the T-arm...

... the bigger insects [ 4 ]. Many important genes, such as toll and imd , have been identified using D. melanogaster [ 4 ]. Caenorhabditis elegans cecropin innate immunity molecular evolution nematode 5′ rapid amplification of cDNA ends (RACE) To screen novel Ascaris transcripts...

... development under laboratory conditions. The TKO worm may be more susceptible to mutations in other genes, thereby providing an approach for the identification of genes that interact with GnT I. Caenorhabditis elegans N -acetylglucosaminyltransferase I null mutations mass spectrometry (MS) N...

...You-Jun FEI; Jin-Cai LIU; Katsuhisa INOUE; Lina ZHUANG; Katsuya MIYAKE; Seiji MIYAUCHI; Vadivel GANAPATHY We have cloned and functionally characterized an Na + -coupled citrate transporter from Caenorhabditis elegans (ceNAC-2). This transporter shows significant sequence homology to Drosophila Indy...

...Christopher J. MEE; Simon R. TOMLINSON; Pavel V. PERESTENKO; David de POMERAI; Ian R. DUCE; Peter N. R. USHERWOOD; David R. BELL Black widow spider venom (BWSV) kills Caenorhabditis elegans after injection owing to the presence of heat- and detergent-sensitive components, which are high-molecular...

...Wenli ZHANG; Pinjiang CAO; Shihao CHEN; Andrew M. SPENCE; Shaoxian ZHU; Erika STAUDACHER; Harry SCHACHTER We have previously reported three Caenorhabditis elegans genes ( gly-12, gly-13 and gly-14 ) encoding UDP- N -acetyl- d -glucosamine:α-3- d -mannoside β1,2- N -acetylglucosaminyltransferase I...

...Wei CHEN; Patricia R. KOMUNIECKI; Richard KOMUNIECKI Pyruvate dehydrogenase kinases (PDKs) from the anaerobic parasitic nematode Ascaris suum and the free-living nematode Caenorhabditis elegans were functionally expressed with hexahistidine tags at their N-termini and purified to apparent...