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Keywords: Clavularia
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Articles

Directed evolution of a monomeric, bright and photostable version of Clavularia cyan fluorescent protein: structural characterization and applications in fluorescence imaging

Hui-wang Ai, J. Nathan Henderson, S. James Remington, Robert E. Campbell
Journal: Biochemical Journal
Biochem J (2006) 400 (3): 531–540.
https://doi.org/10.1042/BJ20060874
Published: 28 November 2006
... CFP (cyan FP) cFP484 from Clavularia coral. Starting from a designed synthetic gene library with mammalian codon preferences, we identified dimeric cFP484 variants with fluorescent brightness significantly greater than the wild-type protein. Following incorporation of dimer-breaking mutations...
View article titled, Directed evolution of a monomeric, bright and photostable version of <span class="search-highlight">Clavularia</span> cyan fluorescent protein: structural characterization and applications in fluorescence imaging
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Includes: Supplementary data