... protein. This mutant kills different insect populations that are resistant to Cry toxins, but lost potency against susceptible insects. We found that the Cry1AbMod-protoxin efficiently induces oligomerization, but not the activated Cry1AbMod-toxin, explaining the loss of potency of Cry1AbMod against...

...Claudia Rodríguez-Almazán; Esmeralda Z. Reyes; Fernando Zúñiga-Navarrete; Carlos Muñoz-Garay; Isabel Gómez; Amy M. Evans; Supaporn Likitvivatanavong; Alejandra Bravo; Sarjeet S. Gill; Mario Soberón Bacillus thuringiensis subsp. israelensis produces three Cry toxins (Cry4Aa, Cry4Ba and Cry11Aa...

... of Bacillus thuringiensis . Though several isoenzymes of aminopeptidases have been identified from the midgut of an insect and their corresponding cDNA cloned, only one of the isoform has been expressed heterologously and studied for its binding to Cry toxins. Here we report the cloning and expression of two...

... with BtR175 in the midgut cells. Advantages are also discussed of applying these transfected mammalian cells to toxicity assays of mutant Cry proteins. Key words: Cry toxin, cytotoxicity assay, d-endotoxin. as Cry receptors by examining whether they can impart Cry susceptibility to heterologous cell systems...