... transfer (trFRET) were used to estimate the amount of DNA bending. Steady-state FRET was measured using a Perkin–Elmer LS 55 Fluorescence Spectrometer at room temperature in a final volume of 50 µl with 50 nM DNA. Assays used 1 μM protein and were incubated for 10 min at room temperature prior to analysis...

... share a similar tertiary structure. Both proteins bind DNA in the minor groove, inducing significant DNA bending and DNA unwinding. Among the most remarkable features of the DNA deformation caused by the two proteins is a single-step sharp kink induced by bound Cren7 (∼50°) or Sul7d (∼60°). The ability...

... specific binding preference for chorion gene promoter elements and induces DNA bending thereon. BmHMGA deploys temporal-specific interaction with transcription factors BmC/EBP (C/EBP is CCAAT/enhancer-binding protein) and BmGATAβ during follicle maturation. The respective protein complexes can be detected...

... To whom correspondence should be addressed (email [email protected] ). 14 2 2005 13 4 2005 22 4 2005 22 4 2005 The Biochemical Society, London 2005 DNA bending DNA site size HBsu integration host factor (IHF) histone-like HU protein salt bridge type II DNA-binding...

.... We also show that, like Sp1 and E-box-binding proteins such as USF1 and USF2, NRF-1 causes DNA bending, in this case producing a bend of 57° towards the major groove. The combined effect of the four protein-induced bends on promoter geometry is the formation of a highly compact arch-like structure...

... correspondence should be addressed (e-mail [email protected]). 12 2 1999 7 4 1999 19 5 1999 The Biochemical Society, London © 1999 1999 bandshift DNA bending DNAprotein complexes potassium permanganate transcription regulation Biochem. J. (1999) 341, 477 482 (Printed in Great...