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Keywords: DNA damage
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Biochem J (2022) 2345–2349.
Published: 23 November 2022
...Elizabeth M. Black; Yoon Ki Joo; Lilian Kabeche Chk1 is a member of the DNA damage response pathway, whose loss leads to replication stress and genome instability. Because of its protective role against lethal levels of DNA replication stress, Chk1 has been studied as a valuable and intriguing...
Biochem J (2022) 479 (13): 1429–1439.
Published: 04 July 2022
... assay. DNA damage is inhibited by growth media containing amino acids, which bind to extracellular Ru and prevent its entry into cells. We conclude that the cytotoxicity of Ru(II) is different from that of platinum, making it a promising development target for cancer therapeutics. Correspondence...
Biochem J (2021) 478 (12): 2359–2370.
Published: 23 June 2021
... 2021 27 5 2021 1 6 2021 1 6 2021 © 2021 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2021 base excision repair DNA damage DNA glycosylase oxidative stress Activation of macrophages and neutrophils during the inflammatory...
Includes: Supplementary data
Biochem J (2021) 478 (7): 1399–1412.
Published: 16 April 2021
...Evgeniy S. Shilkin; Anastasia S. Gromova; Margarita P. Smal; Alena V. Makarova Y-family DNA polymerase iota (Pol ι) is involved in DNA damage response and tolerance. Mutations and altered expression level of POLI gene are linked to a higher incidence of cancer. We biochemically characterized five...
Includes: Supplementary data
Biochem J (2020) 477 (24): 4797–4810.
Published: 24 December 2020
...Hunmin Jung; Michael A. Hawkins; Seongmin Lee The exocyclic amines of nucleobases can undergo deamination by various DNA damaging agents such as reactive oxygen species, nitric oxide, and water. The deamination of guanine and adenine generates the promutagenic xanthine and hypoxanthine...
Biochem J (2019) 476 (21): 3401–3411.
Published: 15 November 2019
... with intrinsic disorder regions. HDM2 is a hub protein with a large interactome and with different cellular functions. It is best known for its regulation of the p53 tumour suppressor. Under normal cellular conditions, HDM2 ubiquitinates and degrades p53 by the 26S proteasome but after DNA damage, HDM2 switches...
Includes: Supplementary data
Biochem J (2015) 465 (3): 413–421.
Published: 22 January 2015
... of Alzheimer's disease (AD) and AICD has important roles in the regulation of gene transcription (in complex with Fe65). It is therefore important to understand how Fe65 is regulated and how this contributes to the function and/or processing of APP. Studies have also implicated Fe65 in the cellular DNA damage...
Biochem J (2012) 448 (1): 115–125.
Published: 18 October 2012
...-strand break DNA damage leukaemia single-strand break V(D)J recombination V(D)J recombination is a site-specific recombination, which generates immunoglobulin and TCR (T-cell receptor) diversity by assembling the variable (V), diversity (D) and joining (J) subexons of the antigen receptor...
Includes: Supplementary data
Biochem J (2012) 443 (1): 213–222.
Published: 14 March 2012
... Sciences, Niger Delta University, Bayelsa State, Nigeria. 2 To whom correspondence should be addressed (email P.J.Thornalley@warwick.ac.uk ). 12 9 2011 20 12 2011 22 12 2011 22 12 2011 © The Authors Journal compilation © 2012 Biochemical Society 2012 DNA damage...
Includes: Supplementary data
Biochem J (2012) 441 (3): 813–821.
Published: 16 January 2012
...) and PLGA [poly(lactic-co-glycolic acid)]-PEO [poly(ethylene oxide)] polymeric NPs (150 nm). We evaluated their uptake by the cells, and their localization, generation of oxidative stress and DNA-damaging effects in exposed cells. We show that NPs are internalized by human brain-derived endothelial cells...
Biochem J (2012) 441 (1): 227–236.
Published: 14 December 2011
... (glycogen synthase kinase 3) phosphorylates RASSF1C to promote RASSF1C degradation subsequently, which is negatively regulated by the PI3K (phosphoinositide 3-kinase)/Akt pathway. Thus the present study reveals a novel regulation of RASSF1C and the potentially important role of RASSF1C in DNA damage...
Includes: Supplementary data
Biochem J (2011) 440 (1): 127–135.
Published: 27 October 2011
... -methyl-rac-glycero-3-phosphocholine), FasL (Fas ligand) and DNA damage. S49 cells made resistant to ALP (S49 AR ) are defective in sphingomyelin synthesis and ALP uptake, and also have acquired resistance to FasL and DNA damage. However, these cells can be re-sensitized following prolonged culturing...
Includes: Supplementary data
Biochem J (2011) 436 (3): 687–698.
Published: 27 May 2011
...Adam J. Koppers; Lisa A. Mitchell; Ping Wang; Minjie Lin; R. John Aitken Human spermatozoa are characterized by poor functionality and abundant DNA damage that collude to generate the high incidences of male infertility and miscarriage seen in our species. Although apoptosis has been suggested...
Biochem J (2011) 435 (1): 65–71.
Published: 15 March 2011
... Society 2011 cell division arrest chromosome mis-segregation DNA damage KillerRed photodynamics optogenetics GFP (green fluorescent protein) and homologous fluor-escent proteins are widely used as genetically encoded fluorescent labels for imaging structures and processes in living...
Includes: Supplementary data
Biochem J (2010) 429 (3): 573–582.
Published: 14 July 2010
... (email giuseppe.villani@ipbs.fr ). 19 3 2010 28 5 2010 8 6 2010 8 6 2010 © The Authors Journal compilation © 2010 Biochemical Society 2010 7,8-dihydro-8-oxoguanine DNA replication DNA polymerase DNA damage proliferating-cell nuclear antigen (PCNA) replication...
Includes: Supplementary data
Biochem J (2010) 428 (2): 147–161.
Published: 13 May 2010
... The Authors Journal compilation © 2010 Biochemical Society 2010 chromosome segregation cohesion DNA damage heterochromatin transcriptional insulation The accurate separation of sister chromatids into daughter cells is critical for maintaining genome integrity. A loss of genome integrity via...
Biochem J (2009) 424 (3): 431–438.
Published: 10 December 2009
.... Apoptosis was the predominant type of cell death, with mechanistic studies revealing that oxidative stress and DNA damage may have a part to play. For the first time, uptake of Ant 4 via the PTS was demonstrated both directly and indirectly in human cell lines. In addition, Ant 4 significantly reduced...
Biochem J (2009) 423 (3): 363–374.
Published: 12 October 2009
... of HUVECs upon knockdown of Nox4. Surprisingly, mean telomere length was significantly reduced in Nox4-depleted cells. Nox4 depletion had no discernable influence on the activity of MAPKs and stress-activated kinases, but reduced the degree of oxidative DNA damage. These results suggest that Nox4 activity...
Includes: Supplementary data
Biochem J (2009) 422 (3): 543–551.
Published: 27 August 2009
...Hanshao Liu; Hoi Chin Hew; Zheng-Guang Lu; Tomoko Yamaguchi; Yoshio Miki; Kiyotsugu Yoshida Transcriptional regulation of the p53 tumour suppressor gene plays an important role in the control of the expression of various target genes involved in the DNA damage response. However, the molecular basis...
Biochem J (2009) 418 (3): 625–634.
Published: 25 February 2009
... that EWS and EWS-Fli1 (Friend leukaemia virus integration 1), the fusion most frequently found in Ewing's sarcomas, become phosphorylated at Thr 79 in response to either mitogens or DNA-damaging agents. The much weaker mitogen-induced phosphorylation of EWS is catalysed by the MAPKs (mitogen-activated...
Biochem J (2008) 415 (2): 297–307.
Published: 25 September 2008
... protein 10] fusion generated by a t(12;16)(q13;p11) chromosomal translocation, Ser 42 in FUS–CHOP is not phosphorylated after DNA damage. These results identify FUS as a new target of the ATM-signalling pathway and strengthen the notion that FUS regulates genome stability. 1 To whom correspondence...
Biochem J (2007) 406 (3): 437–444.
Published: 29 August 2007
... 2007 8 6 2007 © The Authors Journal compilation © 2007 Biochemical Society 2007 chromatin immunoprecipitation DNA damage p53 PC4 promoter transcription The tumour suppressor protein p53 plays a pivotal role in maintenance of cellular homoeostasis. It is a sequence...
Includes: Supplementary data
Biochem J (2007) 405 (1): 115–122.
Published: 13 June 2007
...-mediated procaspase 9 activation under more natural conditions remains unresolved. In the present study, we show that Apaf-1-deficient Jurkat T-lymphocytes and mouse embryonic fibroblasts were highly resistant to DNA-damage-induced apoptosis and failed to cleave or activate any apoptotic procaspase...
Biochem J (2006) 400 (3): 573–582.
Published: 28 November 2006
...Roni H. G. Wright; Edward S. Dornan; Mary M. Donaldson; Iain M. Morgan TopBP1 has eight BRCT [BRCA1 (breast-cancer susceptibility gene 1) C-terminus] domains and is involved in initiating DNA replication, and DNA damage checkpoint signalling and repair. Several BRCT-domain-containing proteins...
Biochem J (2005) 391 (2): 325–333.
Published: 10 October 2005
... is greatly decreased after exposure of cells to DNA damage, indicative of post-translational modification. Here we report that Slx4 is heavily phosphorylated after exposure of cells to a wide range of agents that damage DNA or cause replication fork stalling. Genotoxin-induced Slx4 phosphorylation requires...
Includes: Supplementary data
Biochem J (2005) 388 (3): 813–818.
Published: 07 June 2005
...Ayako FURUKAWA; Yusuke HIRAKU; Shinji OIKAWA; Catherine LUXFORD; Michael J. DAVIES; Shosuke KAWANISHI In γ-irradiation, • OH is directly generated from water and causes DNA damage leading to carcinogenesis. Exposure of proteins to γ-irradiation, in the presence of oxygen, gives high yields...
Biochem J (2005) 388 (2): 705–712.
Published: 24 May 2005
...Catriona A. L. CLARKE; Paul R. CLARKE Cell-cycle checkpoints induced by DNA damage or replication play critical roles in the maintenance of genomic integrity during cell proliferation. Biochemical analysis of checkpoint pathways has been greatly facilitated by the use of cell-free systems made from...
Biochem J (2005) 388 (1): e1.
Published: 10 May 2005
...Benjamin N. WARDLEWORTH; Jessica A. DOWNS In response to DNA damage, cells initiate multiple repair mechanisms that all contribute to the survival of both the cell and the organism. These responses are numerous and variable, and can include cell cycle arrest, transcriptional activation of DNA...
Biochem J (2005) 388 (1): 7–15.
Published: 10 May 2005
... severe to be tolerated. Evidence indicates that, upon generation of DNA DSBs, many nuclear proteins that are involved in DNA repair and checkpoints are recruited to chromatin around the DNA lesions. In the present study we used a proteomics approach to identify DNA-damage-induced chromatin-binding...
Biochem J (2005) 387 (3): 703–710.
Published: 26 April 2005
... rupture. The rapid decrease in ICIP after addition of DFO to the medium suggests draining of cytosolic iron to the medium, rather than penetration of DFO through the plasma membrane. Most importantly, these observations directly connect oxidative stress and resultant DNA damage with lysosomal rupture...
Biochem J (2004) 378 (3): 1039–1045.
Published: 15 March 2004
... lysosomal-membrane integrity against H 2 O 2 -induced oxidative disruption. More importantly, both forms of DFO prevented H 2 O 2 -induced strand breaks in nuclear DNA, including telomeres. To exclude the possibility that lysosomal hydrolases, rather than iron, caused the observed DNA damage, limited...
Biochem J (2003) 376 (3): 725–732.
Published: 15 December 2003
... pathways exhibit cross-regulation in cytokine-dependent, but not in p53-dependent, cell-death models. apoptosis ceramide DNA damage glutathione p53 signal transduction Abbreviations used: BSO, l -buthionine S,R -sulphoximine; C 6 ceramide, N -hexanoylsphingosine; CHX, cycloheximide; ER...
Biochem J (2003) 371 (2): 301–310.
Published: 15 April 2003
... 2003 The Biochemical Society, London ©2003 2003 apoptosis cell-cycle arrest DNA damage tumour suppressor gene Abbreviations used: CTF, CCAAT-binding transcription factor; DTT, dithiothreitol; ECL ® , enhanced chemiluminescence; EMSA, electrophoretic mobility shift assay; GST...
Biochem J (2002) 365 (3): 605–615.
Published: 01 August 2002
... 2002 The Biochemical Society, London ©2002 2002 DNA damage free radicals hypochlorite myeloperoxidase spin trapping Abbreviations used: DMPO, 5,5-dimethyl-1-pyrroline- N -oxide; DTBN, di- t -butylnitroxide; MNP, 2-methyl-2-nitrosopropane; NAH, N -α-acetyl-histidine; TNB, 5...
Biochem J (2001) 359 (2): 459–464.
Published: 08 October 2001
... by Mdm2 (mouse double minute clone 2), a negative regulatory partner of p53. Previous studies have suggested that DNA-damage-induced phosphorylation of p53 at key N-terminal sites has a pivotal role in regulating the interaction with Mdm2 but the precise role of phosphorylation of serines 15 and 20...
Biochem J (2001) 359 (1): 183–192.
Published: 24 September 2001
..., such as hypoxia or heat shock. Induction of genotoxic stress also results in a large reduction in global protein synthesis rates and therefore we investigated whether the c- myc IRES was active following DNA damage. As expected, in cells treated with either ethylmethane sulphonate or mitomycin C there was a large...
Biochem J (2001) 356 (2): 509–513.
Published: 24 May 2001
... with dehydroascorbic acid, the vitamin present in the cells was all in its reduced form. It was found that, in cells that are otherwise ascorbate-deficient, an increase in their ascorbic acid content does not prevent, but rather enhances, the DNA-damaging and lethal responses mediated by exogenous ONOO...
Biochem J (2001) 355 (2): 473–479.
Published: 06 April 2001
... (MTGSH cells). Cell survival following exposure to oxidative stress and chemical agents was higher in cells expressing the native MT gene than in cells where MT localization was disrupted, or in untransfected cells. Also, MTMT cells showed less DNA damage than MTGSH cells in response to either hydrogen...
Biochem J (2000) 352 (1): 1–17.
Published: 07 November 2000
... properties of p53 protein that affect its tumour-suppressor function and the experimental strategies that have been developed for the re- activation of the p53 pathway in cancers. Key words: cell-cycle checkpoint pathway, degradation, DNA damage, transcription. THE TRANSACTIVATION FUNCTION OF p53 PROTEIN...
Biochem J (1999) 344 (1): 125–134.
Published: 08 November 1999
... give rise to subsequent DNA damage; the latter includes both DNA-protein cross-links and formation of oxidized DNA bases. 1 To whom correspondence should be addressed (e-mail m.davies@hri.org.au). 14 6 1999 16 8 1999 10 9 1999 The Biochemical Society, London © 1999 1999...