... sensitive to calcium with different intensities, whereas six CPKs from subgroup 3 exhibited low or no calcium sensitivity to two generic substrates. Interestingly, this apparent calcium-independence correlated with significant alterations in the predicted EF-hands of these kinases, although they all bound...

...Liliana Santamaria-Kisiel; Gary S. Shaw S100A11 is a dimeric EF-hand calcium-binding protein. Calcium binding to S100A11 results in a large conformational change that uncovers a broad hydrophobic surface used to interact with phospholipid-binding proteins (annexins A1 and A2) and facilitate...

... a series of mutant CaMs designed to individually increase the Ca 2+ affinity of each of CaM's EF-hands by increasing the number of acidic residues in Ca 2+ -chelating positions. Domain-specific Ca 2+ affinities of each CaM variant were determined by equilibrium fluorescence titration. Mutations in sites I...

... was then developed using ECL® (enhanced chemiluminescence) reagents (Amersham). calcium EF-hand exocytosis Na + /Ca 2+ exchanger neuronal calcium sensor (NCS) protein neurotransmitter release The transduction of intracellular Ca 2+ signals into physiological responses is mediated by Ca 2+ sensor...

...Jessica L. Gifford; Michael P. Walsh; Hans J. Vogel The ‘EF-hand’ Ca 2+ -binding motif plays an essential role in eukaryotic cellular signalling, and the proteins containing this motif constitute a large and functionally diverse family. The EF-hand is defined by its helix–loop–helix secondary...

...Annika Rogstam; Sara Linse; Anders Lindqvist; Peter James; Ludwig Wagner; Tord Berggård Secretagogin is a hexa EF-hand protein, which has been identified as a novel potential tumour marker. In the present study, we show that secretagogin binds four Ca 2+ ions (log K 1 =7.1±0.4, log K 2 =4.7±0.6...

...Liliana Santamaria-Kisiel; Anne C. Rintala-Dempsey; Gary S. Shaw The S100 proteins comprise at least 25 members, forming the largest group of EF-hand signalling proteins in humans. Although the proteins are expressed in many tissues, each S100 protein has generally been shown to have a preference...

... on SDS/PAGE (10% gel) [ 23 ] and was used for the immunization of rabbits. 1 To whom correspondence should be addressed (email [email protected] ). 16 9 2005 3 1 2006 24 1 2006 24 1 2006 Cab45/reticulocalbin/ERC45/calumenin (CREC) EF-hand paired basic...

...Keiichi Katoh; Hidenori Suzuki; Yoshinori Terasawa; Takako Mizuno; Jiro Yasuda; Hideki Shibata; Masatoshi Maki ALG-2 (apoptosis-linked gene 2) is a Ca 2+ -binding protein that belongs to the PEF (penta-EF-hand) protein family. Alix (ALG-2-interacting protein X)/AIP1 (ALG-2-interacting protein 1...

...Christian VANNAHME; Silke GÖSLING; Mats PAULSSON; Patrik MAURER; Ursula HARTMANN We have isolated the novel gene SMOC-2, which encodes a secreted modular protein containing an EF-hand calcium-binding domain homologous to that in BM-40. It further consists of two thyroglobulin-like domains...

... activated calpain. On the basis of sequence comparison and the X-ray structure of m-calpain, many m-type residues in the C-terminal EF-hand-containing domain IV were converted into the corresponding μ -type residues, but these mutations did not produce the expected decrease in K d . In a series of hybrid...