... sites. The constructs were used to express Ncap proteins and protocols developed which allow efficient production of purified Ncap with yields of over 200 mg per litre of culture media. These proteins were deployed in ELISA assays to allow comparison of their responses to human sera. Our results suggest...

... is the concentration of ligand at which half-maximal specific saturable binding is attained [ 36 , 37 ]. carbohydrate-recognition domain cell adhesion collagen ELISA fibronectin type II domain mannose receptor The macrophage mannose receptor plays a well-documented role in mediating the clearance...

... at the pituitary gonadal axis. ELISA demonstrated that PSPBP levels were significantly lower ( P =0.0014) in the serum of a prostate cancer population ( n =65) compared with a control population ( n =70). PSPBP identification will help the understanding of PSP94's functions and facilitate ELISA development...

...Tao PAN; Boon-Seng WONG; Tong LIU; Ruliang LI; Robert B. PETERSEN; Man-Sun SY We used ELISA and flow cytometry to study the binding of prion protein PrP to glycosaminoglycans (GAGs). We found that recombinant human PrP (rPrP) binds GAGs including chondroitin sulphate A, chondroitin sulphate B...

...Aneet K. SIAN; Emma R. FREARS; Omar M. A. EL-AGNAF; Bhroma P. PATEL; Maria F. MANCA; Giuliano SILIGARDI; Rohanah HUSSAIN; Brian M. AUSTEN A novel ELISA has been developed which detects oligomerization of β-amyloid (Aβ). Oligomerization, fibrillization and neurotoxicity of native Aβ associated...

...), the C-terminus (14.4 kDa) and intact Fn by ELISA yielded estimated K d values of 216, 18 and 2.1 nM, respectively. The higher fibrin-binding affinity of the N-terminus was substantiated by the ability of both a recombinant 4 F1. 5 F1 and a monoclonal antibody (mAb) to this site to maximally inhibit...

... antibodies to cells. A sandwich ELISA was set up using mAb 131.4 (directed against purified CD36 from human platelets) as capture antibody and phage antibodies and anti-phage sera as detector. With this ELISA (sensitivity 0.05 µ g/ml), the FAT content in isolated cardiomyocytes was found to be comparable...