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Keywords: Escherichia coli
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Biochem J (2021) 478 (4): 855–869.
Published: 24 February 2021
... was measured in Escherichia coli using DAF-FM. Using the properties of NO passing through plasma membrane easily, increased malondialdehyde levels have shown that lupeol causes lipid peroxidation, and the resulting membrane depolarization was confirmed by DiBAC 4 (3). These data indicated that lupeol...
Biochem J (2018) 475 (8): 1397–1410.
Published: 23 April 2018
... changes, and understanding the biosynthetic pathways is important. We identified novel amino acid racemases possessing the ability to produce non-canonical d -amino acids in Escherichia coli and Bacillus subtilis in our previous study, whereas the biosynthetic pathways of these d -amino acids still remain...
Biochem J (2017) 474 (18): 3189–3205.
Published: 08 September 2017
... ATPase cell division cytoskeleton Escherichia coli FtsZ MinD Cell division in bacteria is a highly controlled and regulated process. More than a dozen of proteins are associated with the cell division. Among these, FtsZ, a bacterial cytoskeletal protein, forms the skeleton of a ring-like...
Includes: Multimedia, Supplementary data
Biochem J (2017) 474 (8): 1395–1416.
Published: 04 April 2017
...Cora Lilia Alvarez; Gerardo Corradi; Natalia Lauri; Irene Marginedas-Freixa; María Florencia Leal Denis; Nicolás Enrique; Sabina María Mate; Verónica Milesi; Mariano Anibal Ostuni; Vanesa Herlax; Pablo Julio Schwarzbaum We studied the kinetics of extracellular ATP (ATPe) in Escherichia coli and...
Includes: Supplementary data
Biochem J (2015) 472 (2): 205–216.
Published: 13 November 2015
...Isabelle Petit-Härtlein; Kevin Rome; Eve de Rosny; Florian Molton; Carole Duboc; Erwan Gueguen; Agnès Rodrigue; Jacques Covès The ZraSR system belongs to the family of TCSs (two-component signal transduction systems). In Escherichia coli , it was proposed to participate in zinc balance and to...
Includes: Supplementary data
Biochem J (2013) 454 (3): 543–549.
Published: 29 August 2013
... S . Typhimurium SL1344 [ 10 ] and Escherichia coli 83972 [ 11 ] were used in the present study. The deletion mutants constructed in these two strains are described in Table 1 . The SL1344 entC and iroB mutants were described previously [ 12 ]. In the present study, derivatives of these...
Includes: Supplementary data
Biochem J (2013) 452 (1): 57–66.
Published: 25 April 2013
... Escherichia coli , Tat-dependent proteins often contain redox cofactors that must be loaded before translocation. Trimethylamine N -oxide reductase (TorA) is a model bacterial Tat substrate and is a molybdenum cofactor-dependent enzyme. Co-ordination of cofactor loading and translocation of TorA is directed...
Includes: Supplementary data
Biochem J (2013) 449 (2): 333–341.
Published: 14 December 2012
...Chiara Saggioro; Anne Olliver; Bianca Sclavi The DnaA protein is a key factor for the regulation of the timing and synchrony of initiation of bacterial DNA replication. The transcription of the dnaA gene in Escherichia coli is regulated by two promoters, dnaAP1 and dnaAP2 . The region between these...
Includes: Supplementary data
Biochem J (2012) 448 (3): 329–341.
Published: 21 November 2012
...Abbas Maqbool; Mireille Hervé; Dominique Mengin-Lecreulx; Anthony J. Wilkinson; Gavin H. Thomas The murein peptide amidase MpaA is a cytoplasmic enzyme that processes peptides derived from the turnover of murein. We have purified the enzyme from Escherichia coli and demonstrated that it efficiently...
Includes: Supplementary data
Biochem J (2012) 444 (3): 553–560.
Published: 29 May 2012
... previously thought not to play a role in iron core formation. Investigation of the effect of bound haem on formation of the iron core has been enabled in the present work by development of a method for reconstitution of BFR from Escherichia coli with exogenously added haem at elevated temperature in the...
Biochem J (2012) 441 (1): 481–485.
Published: 14 December 2011
...María-Antonia Sánchez-Romero; David J. Lee; Eugenio Sánchez-Morán; Stephen J. W. Busby In the present paper, we report that transcription affects the location of a DNA target in Escherichia coli K-12. A strain whose chromosome had been engineered to encode a lac repressor–GFP (green fluorescent...
Biochem J (2011) 433 (1): 107–117.
Published: 15 December 2010
...Manuel Montero; Goizeder Almagro; Gustavo Eydallin; Alejandro M. Viale; Francisco J. Muñoz; Abdellatif Bahaji; Jun Li; Mehdi Rahimpour; Edurne Baroja-Fernández; Javier Pozueta-Romero Although it is generally accepted that Escherichia coli glycogen genes are organized in two tandemly arranged...
Includes: Supplementary data
Biochem J (2010) 430 (1): 61–68.
Published: 28 July 2010
...David L. Chismon; Douglas F. Browning; Gregory K. Farrant; Stephen J. W. Busby Expression from the Escherichia coli hcp - hcr operon promoter is optimally induced during anaerobic conditions in the presence of nitrite. This expression depends on transcription activation by FNR (fumarate and nitrate...
Biochem J (2008) 415 (3): 449–454.
Published: 15 October 2008
...Jürgen U. Linder Class I adenylate cyclases are found in γ- and δ-proteobacteria. They play central roles in processes such as catabolite repression in Escherichia coli or development of full virulence in pathogens such as Yersinia enterocolitica and Vibrio vulnificus . The catalytic domain...
Includes: Supplementary data
Biochem J (2008) 410 (1): 39–48.
Published: 29 January 2008
... between the catalytic sites and the requirement for both dsRBDs for processing activity are not known. It is shown that an Escherichia coli RNase III heterodimer that contains a single functional wt (wild-type) catalytic site and an inactive catalytic site (RNase III[E117A/wt]) cleaves a substrate with a...
Biochem J (2008) 409 (1): 129–137.
Published: 11 December 2007
... mtDNA (mitochondrial DNA), leading to amino acid substitutions in mitochondrial complex I subunits ND1, ND4 and ND6 respectively. We have now examined the effects of ND6 mutations on the function of complex I using the homologous NuoJ subunit of Escherichia coli NDH-1 (NADH:quinone oxidoreductase) as a...
Includes: Supplementary data
Biochem J (2007) 404 (3): 477–485.
Published: 29 May 2007
...Francesca Malerba; Andrea Bellelli; Alessandra Giorgi; Francesco Bossa; Roberto Contestabile Previous studies suggest that the addition of pyridoxal 5′-phosphate to apo-serine hydroxymethyltransferase from Escherichia coli is the last event in the enzyme's folding process. We propose a mechanism...
Biochem J (2007) 403 (1): 207–215.
Published: 13 March 2007
...Mirijam-Elisabeth Zeller; Agnes Csanadi; Andras Miczak; Thierry Rose; Thierry Bizebard; Vladimir R. Kaberdin The RNase E/G family of endoribonucleases plays the central role in numerous post-transcriptional mechanisms in Escherichia coli and, presumably, in other bacteria, including human pathogens...
Biochem J (2006) 396 (2): 227–234.
Published: 15 May 2006
...Ferenc Marincs; Iain W. Manfield; Jonathan A. Stead; Kenneth J. Mcdowall; Peter G. Stockley We have used DNA arrays to investigate the effects of knocking out the methionine repressor gene, metJ , on the Escherichia coli transcriptome. We assayed the effects in the knockout strain of supplying wild...
Includes: Supplementary data
Biochem J (2006) 395 (2): 427–432.
Published: 28 March 2006
...Timothy C. Meredith; Ronald W. Woodard In Escherichia coli , there are multiple paralogous copies of the enzyme API [A5P ( D -arabinose 5-phosphate) isomerase], which catalyses the conversion of the pentose pathway intermediate Ru5P ( D -ribulose 5-phosphate) into A5P. A5P is a precursor of Kdo (3...
Biochem J (2006) 393 (3): 767–777.
Published: 13 January 2006
... remains unclear. To clarify this, two RRF chimaeras, Eco DI/ Tte DII and Tte DI/ Eco DII, were created by domain swaps between the proteins from Escherichia coli and Thermoanaerobacter tengcongensis . The ribosome recycling activity of the RRF chimaeras was compared with their wild-type RRFs by using in...
Biochem J (2005) 392 (1): 55–63.
Published: 08 November 2005
...George Nicola; Alena Fedarovich; Robert A. Nicholas; Christopher Davies Penicillin-binding proteins (PBPs), which are the lethal targets of β-lactam antibiotics, catalyse the final stages of peptidoglycan biosynthesis of the bacterial cell wall. PBP 5 of Escherichia coli is a D -alanine CPase...
Biochem J (2005) 389 (3): 885–898.
Published: 26 July 2005
...Mariarita Bertoldi; Barbara Cellini; Douglas V. Laurents; Carla Borri Voltattorni MalY from Escherichia coli is a bifunctional dimeric PLP (pyridoxal 5′-phosphate) enzyme acting as a β-cystathionase and as a repressor of the maltose system. The spectroscopic and molecular properties of the...
Biochem J (2005) 388 (3): 879–887.
Published: 07 June 2005
...Daniela K. JACQUELÍN; Adrián FILIBERTI; Carlos E. ARGARAÑA; José L. BARRA Escherichia coli MutS, MutL and MutH proteins act sequentially in the MMRS (mismatch repair system). MutH directs the repair system to the newly synthesized strand due to its transient lack of Dam (DNA-adenine methylase...
Biochem J (2005) 386 (3): 489–495.
Published: 08 March 2005
...Houssain BENABDELHAK; Lutz SCHMITT; Carsten HORN; Kornelia JUMEL; Mark A. BLIGHT; I. Barry HOLLAND The ATPase activity of the ABC (ATP-binding cassette) ATPase domain of the HlyB (haemolysin B) transporter is required for secretion of Escherichia coli haemolysin via the type I pathway. Although ABC...
Biochem J (2004) 384 (3): 577–584.
Published: 07 December 2004
...Abdellah ALLALI-HASSANI; Tracey L. CAMPBELL; Andy HO; Jeffrey W. SCHERTZER; Eric D. BROWN In the study described here, we have taken steps to characterize the YjeE protein, an Escherichia coli protein of unknown function that is essential for bacterial viability. YjeE represents a protein family...
Biochem J (2004) 383 (2): 371–382.
Published: 08 October 2004
...Benoit THOUVENOT; Bruno CHARPENTIER; Christiane BRANLANT The Escherichia coli multi-promoter region of the gapA gene ensures a high level of GAPDH (glyceraldehyde-3-phosphate dehydrogenase) production under various growth conditions. In the exponential phase of growth, gapA mRNAs are mainly...
Biochem J (2004) 380 (3): 859–865.
Published: 15 June 2004
... evidence that many of them interact and increase the permeability of bacterial membranes as part of their killing mechanism. However, it is not clear whether this is the lethal step. To address this issue, we studied the interaction of the antimicrobial peptide temporin L with Escherichia coli by using...
Biochem J (2003) 374 (3): 767–772.
Published: 15 September 2003
...Elisabet ROMAN; Ian ROBERTS; Kerstin LIDHOLT; Marion KUSCHE-GULLBERG The Escherichia coli K5 capsular polysaccharide (glycosaminoglycan) chains are composed of the repeated disaccharide structure: -GlcAβ1,4-GlcNAcα1,4-(where GlcA is glucuronic acid and GlcNAc is N -acetyl- d -glucosamine). The GlcA...
Biochem J (2003) 370 (3): 867–871.
Published: 15 March 2003
...Monika SLOMINSKA; Anne WAHL; Grzegorz WEGRZYN; Kirsten SKARSTAD A mutant form of the Escherichia coli replication initiator protein, DnaA204, is unstable. At low growth rates, the dnaA204 mutant cells experience a limitation of initiator protein and grow with reduced initiation frequency and DNA...
Biochem J (2002) 367 (2): 491–497.
Published: 15 October 2002
... Escherichia coli as a His-tagged protein. The absorption spectrum of the purified recombinant protein is typical of flavoenzymes. Furthermore, it showed NADPH-dependent thioredoxin h reduction activity, thus confirming that the cDNA clone reported in the present study encodes wheat NTR. Using the His-tagged...
Biochem J (2002) 362 (2): 453–458.
Published: 22 February 2002
...Masaki MAKISE; Shinji MIMA; Motohiro KOTERASAWA; Tomofusa TSUCHIYA; Tohru MIZUSHIMA The DnaA protein is the initiator of chromosomal DNA replication in Escherichia coli . Acidic phospholipids decrease its affinity for adenine nucleotides, and re-activate the ADP-bound form to the ATP-bound form. We...
Biochem J (2001) 358 (2): 505–510.
Published: 24 August 2001
...Stephane HAZEBROUCK; Valerie MACHTELINCKX-DELMAS; Jean-Jacques KUPIEC; Pierre SONIGO Insertional mutagenesis of the Escherichia coli thymidylate synthase (TS) was used to address substrate recognition of HIV-1 protease in a well characterized structural context. By modifying the TS conformation...
Biochem J (2001) 357 (3): 779–785.
Published: 25 July 2001
... 17 5 2001 8 6 2001 The Biochemical Society, London ©2001 2001 affinity chromatography Escherichia coli lamB phage lambda receptor peptide library Abbreviations used: CABS, continuous affinity-based selection; OMP, outer membrane protein; R-ve, receptor negative; R...
Biochem J (2000) 348 (2): 375–379.
Published: 23 May 2000
...@pheasant.pharm.okayama-u.ac.jp). 7 2 2000 10 3 2000 29 3 2000 The Biochemical Society, London © 2000 2000 DNA replication DnaA protein Escherichia coli linking number nalidixic acid Biochem. J. (2000) 348, 375 379 (Printed in Great Britain) 375 Suppression of temperature-sensitivity...
Biochem J (2000) 346 (3): 737–742.
Published: 07 March 2000
...Lionel CHARON; Jean-François HOEFFLER; Catherine PALE-GROSDEMANGE; Luisa-Maria LOIS; Narciso CAMPOS; Albert BORONAT; Michel ROHMER Escherichia coli synthesizes its isoprenoids via the mevalonate-independent 2- C -methyl- D -erythritol 4-phosphate (MEP) pathway. The MC4100 dxs ::CAT strain...
Biochem J (1999) 344 (2): 397–402.
Published: 24 November 1999
... sequence, confirms the N-terminal amino acid sequencing data and contains the sequence of an internal tryptic peptide. It gave a calculated M r of 20364. The gene was expressed in Escherichia coli and yielded active enzyme. The derived amino acid sequence does not show significant similarity to other...
Biochem J (1999) 340 (2): 433–438.
Published: 25 May 1999
...@pheasant.pharm.okayama-u.ac.jp). 4 12 1998 3 2 1999 25 2 1999 The Biochemical Society, London © 1999 1999 basic amino acid Escherichia coli initiator protein membrane binding site-directed mutagenesis Biochem. J. (1999) 340, 433 438 (Printed in Great Britain) 433 Involvement of Arg...