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1-39 of 39
Keywords: Escherichia coli
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Articles
Journal:
Biochemical Journal
Biochem J (2025) 482 (12): BCJ20253056.
Published: 11 June 2025
...Neha Pandey; Chitra Seetharam Misra; Devashish Rath The structural and mechanistic complexity of Escherichia coli’ s type I Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated (CRISPR-Cas) system, compared with the multidomain, single effector protein-based type II systems...
Includes: Supplementary data
Articles
Lupeol-induced nitric oxide elicits apoptosis-like death within Escherichia coli in a DNA fragmentation-independent manner
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Journal:
Biochemical Journal
Biochem J (2021) 478 (4): 855–869.
Published: 24 February 2021
... NO was measured in Escherichia coli using DAF-FM. Using the properties of NO passing through plasma membrane easily, increased malondialdehyde levels have shown that lupeol causes lipid peroxidation, and the resulting membrane depolarization was confirmed by DiBAC 4 (3). These data indicated that lupeol-induced...
Articles
Cystathionine β-lyase is involved in d-amino acid metabolism
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Journal:
Biochemical Journal
Biochem J (2018) 475 (8): 1397–1410.
Published: 23 April 2018
... changes, and understanding the biosynthetic pathways is important. We identified novel amino acid racemases possessing the ability to produce non-canonical d -amino acids in Escherichia coli and Bacillus subtilis in our previous study, whereas the biosynthetic pathways of these d -amino acids still remain...
Articles
MinD directly interacting with FtsZ at the H10 helix suggests a model for robust activation of MinC to destabilize FtsZ polymers
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Journal:
Biochemical Journal
Biochem J (2017) 474 (18): 3189–3205.
Published: 08 September 2017
.... Correspondence: Tushar Kant Beuria ( [email protected] ) 12 5 2017 10 7 2017 25 7 2017 25 7 2017 © 2017 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2017 ATPase cell division cytoskeleton Escherichia coli FtsZ MinD...
Includes: Multimedia, Supplementary data
Articles
Dynamic regulation of extracellular ATP in Escherichia coli
Available to PurchaseCora Lilia Alvarez, Gerardo Corradi, Natalia Lauri, Irene Marginedas-Freixa, María Florencia Leal Denis, Nicolás Enrique, Sabina María Mate, Verónica Milesi, Mariano Anibal Ostuni, Vanesa Herlax, Pablo Julio Schwarzbaum
Journal:
Biochemical Journal
Biochem J (2017) 474 (8): 1395–1416.
Published: 04 April 2017
...Cora Lilia Alvarez; Gerardo Corradi; Natalia Lauri; Irene Marginedas-Freixa; María Florencia Leal Denis; Nicolás Enrique; Sabina María Mate; Verónica Milesi; Mariano Anibal Ostuni; Vanesa Herlax; Pablo Julio Schwarzbaum We studied the kinetics of extracellular ATP (ATPe) in Escherichia coli...
Includes: Supplementary data
Articles
Biophysical and physiological characterization of ZraP from Escherichia coli , the periplasmic accessory protein of the atypical ZraSR two-component system
Available to PurchaseIsabelle Petit-Härtlein, Kevin Rome, Eve de Rosny, Florian Molton, Carole Duboc, Erwan Gueguen, Agnès Rodrigue, Jacques Covès
Journal:
Biochemical Journal
Biochem J (2015) 472 (2): 205–216.
Published: 13 November 2015
...Isabelle Petit-Härtlein; Kevin Rome; Eve de Rosny; Florian Molton; Carole Duboc; Erwan Gueguen; Agnès Rodrigue; Jacques Covès The ZraSR system belongs to the family of TCSs (two-component signal transduction systems). In Escherichia coli , it was proposed to participate in zinc balance...
Includes: Supplementary data
Articles
An antioxidant role for catecholate siderophores in Salmonella
Available to PurchaseMaud E. S. Achard, Kaiwen W. Chen, Matthew J. Sweet, Rebecca E. Watts, Kate Schroder, Mark A. Schembri, Alastair G. McEwan
Journal:
Biochemical Journal
Biochem J (2013) 454 (3): 543–549.
Published: 29 August 2013
... for siderophores during host–pathogen interactions. S . Typhimurium SL1344 [ 10 ] and Escherichia coli 83972 [ 11 ] were used in the present study. The deletion mutants constructed in these two strains are described in Table 1 . The SL1344 entC and iroB mutants were described previously [ 12...
Includes: Supplementary data
Articles
Characterization of a pre-export enzyme–chaperone complex on the twin-arginine transport pathway
Open Access
Journal:
Biochemical Journal
Biochem J (2013) 452 (1): 57–66.
Published: 25 April 2013
.... In Escherichia coli , Tat-dependent proteins often contain redox cofactors that must be loaded before translocation. Trimethylamine N -oxide reductase (TorA) is a model bacterial Tat substrate and is a molybdenum cofactor-dependent enzyme. Co-ordination of cofactor loading and translocation of TorA is directed...
Includes: Supplementary data
Articles
Temperature-dependence of the DnaA–DNA interaction and its effect on the autoregulation of dnaA expression
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Journal:
Biochemical Journal
Biochem J (2013) 449 (2): 333–341.
Published: 14 December 2012
...Chiara Saggioro; Anne Olliver; Bianca Sclavi The DnaA protein is a key factor for the regulation of the timing and synchrony of initiation of bacterial DNA replication. The transcription of the dnaA gene in Escherichia coli is regulated by two promoters, dnaAP1 and dnaAP2 . The region between...
Includes: Supplementary data
Articles
MpaA is a murein-tripeptide-specific zinc carboxypeptidase that functions as part of a catabolic pathway for peptidoglycan-derived peptides in γ-proteobacteria
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Journal:
Biochemical Journal
Biochem J (2012) 448 (3): 329–341.
Published: 21 November 2012
...Abbas Maqbool; Mireille Hervé; Dominique Mengin-Lecreulx; Anthony J. Wilkinson; Gavin H. Thomas The murein peptide amidase MpaA is a cytoplasmic enzyme that processes peptides derived from the turnover of murein. We have purified the enzyme from Escherichia coli and demonstrated that it efficiently...
Includes: Supplementary data
Articles
Fe-haem bound to Escherichia coli bacterioferritin accelerates iron core formation by an electron transfer mechanism
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Journal:
Biochemical Journal
Biochem J (2012) 444 (3): 553–560.
Published: 29 May 2012
... and was previously thought not to play a role in iron core formation. Investigation of the effect of bound haem on formation of the iron core has been enabled in the present work by development of a method for reconstitution of BFR from Escherichia coli with exogenously added haem at elevated temperature...
Articles
Journal:
Biochemical Journal
Biochem J (2012) 441 (1): 481–485.
Published: 14 December 2011
...María-Antonia Sánchez-Romero; David J. Lee; Eugenio Sánchez-Morán; Stephen J. W. Busby In the present paper, we report that transcription affects the location of a DNA target in Escherichia coli K-12. A strain whose chromosome had been engineered to encode a lac repressor–GFP (green fluorescent...
Articles
Escherichia coli glycogen genes are organized in a single glgBXCAP transcriptional unit possessing an alternative suboperonic promoter within glgC that directs glgAP expression
Available to PurchaseManuel Montero, Goizeder Almagro, Gustavo Eydallin, Alejandro M. Viale, Francisco J. Muñoz, Abdellatif Bahaji, Jun Li, Mehdi Rahimpour, Edurne Baroja-Fernández, Javier Pozueta-Romero
Journal:
Biochemical Journal
Biochem J (2011) 433 (1): 107–117.
Published: 15 December 2010
...Manuel Montero; Goizeder Almagro; Gustavo Eydallin; Alejandro M. Viale; Francisco J. Muñoz; Abdellatif Bahaji; Jun Li; Mehdi Rahimpour; Edurne Baroja-Fernández; Javier Pozueta-Romero Although it is generally accepted that Escherichia coli glycogen genes are organized in two tandemly arranged...
Includes: Supplementary data
Articles
Unusual organization, complexity and redundancy at the Escherichia coli hcp-hcr operon promoter
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Journal:
Biochemical Journal
Biochem J (2010) 430 (1): 61–68.
Published: 28 July 2010
...David L. Chismon; Douglas F. Browning; Gregory K. Farrant; Stephen J. W. Busby Expression from the Escherichia coli hcp - hcr operon promoter is optimally induced during anaerobic conditions in the presence of nitrite. This expression depends on transcription activation by FNR (fumarate and nitrate...
Articles
Structure–function relationships in Escherichia coli adenylate cyclase
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Journal:
Biochemical Journal
Biochem J (2008) 415 (3): 449–454.
Published: 15 October 2008
...Jürgen U. Linder Class I adenylate cyclases are found in γ- and δ-proteobacteria. They play central roles in processes such as catabolite repression in Escherichia coli or development of full virulence in pathogens such as Yersinia enterocolitica and Vibrio vulnificus . The catalytic domain...
Includes: Supplementary data
Articles
Heterodimer-based analysis of subunit and domain contributions to double-stranded RNA processing by Escherichia coli RNase III in vitro
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Journal:
Biochemical Journal
Biochem J (2008) 410 (1): 39–48.
Published: 29 January 2008
... between the catalytic sites and the requirement for both dsRBDs for processing activity are not known. It is shown that an Escherichia coli RNase III heterodimer that contains a single functional wt (wild-type) catalytic site and an inactive catalytic site (RNase III[E117A/wt]) cleaves a substrate...
Articles
Leber hereditary optic neuropathy mutations in the ND6 subunit of mitochondrial complex I affect ubiquinone reduction kinetics in a bacterial model of the enzyme
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Journal:
Biochemical Journal
Biochem J (2008) 409 (1): 129–137.
Published: 11 December 2007
... mtDNA (mitochondrial DNA), leading to amino acid substitutions in mitochondrial complex I subunits ND1, ND4 and ND6 respectively. We have now examined the effects of ND6 mutations on the function of complex I using the homologous NuoJ subunit of Escherichia coli NDH-1 (NADH:quinone oxidoreductase...
Includes: Supplementary data
Articles
The mechanism of addition of pyridoxal 5′-phosphate to Escherichia coli apo-serine hydroxymethyltransferase
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Journal:
Biochemical Journal
Biochem J (2007) 404 (3): 477–485.
Published: 29 May 2007
...Francesca Malerba; Andrea Bellelli; Alessandra Giorgi; Francesco Bossa; Roberto Contestabile Previous studies suggest that the addition of pyridoxal 5′-phosphate to apo-serine hydroxymethyltransferase from Escherichia coli is the last event in the enzyme's folding process. We propose a mechanism...
Articles
Quaternary structure and biochemical properties of mycobacterial RNase E/G
Available to PurchaseMirijam-Elisabeth Zeller, Agnes Csanadi, Andras Miczak, Thierry Rose, Thierry Bizebard, Vladimir R. Kaberdin
Journal:
Biochemical Journal
Biochem J (2007) 403 (1): 207–215.
Published: 13 March 2007
...Mirijam-Elisabeth Zeller; Agnes Csanadi; Andras Miczak; Thierry Rose; Thierry Bizebard; Vladimir R. Kaberdin The RNase E/G family of endoribonucleases plays the central role in numerous post-transcriptional mechanisms in Escherichia coli and, presumably, in other bacteria, including human pathogens...
Articles
Transcript analysis reveals an extended regulon and the importance of protein–protein co-operativity for the Escherichia coli methionine repressor
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Journal:
Biochemical Journal
Biochem J (2006) 396 (2): 227–234.
Published: 15 May 2006
...Ferenc Marincs; Iain W. Manfield; Jonathan A. Stead; Kenneth J. Mcdowall; Peter G. Stockley We have used DNA arrays to investigate the effects of knocking out the methionine repressor gene, metJ , on the Escherichia coli transcriptome. We assayed the effects in the knockout strain of supplying wild...
Includes: Supplementary data
Articles
Characterization of Escherichia coliD-arabinose 5-phosphate isomerase encoded by kpsF: implications for group 2 capsule biosynthesis
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Journal:
Biochemical Journal
Biochem J (2006) 395 (2): 427–432.
Published: 28 March 2006
...Timothy C. Meredith; Ronald W. Woodard In Escherichia coli , there are multiple paralogous copies of the enzyme API [A5P ( D -arabinose 5-phosphate) isomerase], which catalyses the conversion of the pentose pathway intermediate Ru5P ( D -ribulose 5-phosphate) into A5P. A5P is a precursor of Kdo (3...
Articles
Domain II plays a crucial role in the function of ribosome recycling factor
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Journal:
Biochemical Journal
Biochem J (2006) 393 (3): 767–777.
Published: 13 January 2006
... remains unclear. To clarify this, two RRF chimaeras, Eco DI/ Tte DII and Tte DI/ Eco DII, were created by domain swaps between the proteins from Escherichia coli and Thermoanaerobacter tengcongensis . The ribosome recycling activity of the RRF chimaeras was compared with their wild-type RRFs by using...
Articles
A large displacement of the SXN motif of Cys 115 -modified penicillin-binding protein 5 from Escherichia coli
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Journal:
Biochemical Journal
Biochem J (2005) 392 (1): 55–63.
Published: 08 November 2005
...George Nicola; Alena Fedarovich; Robert A. Nicholas; Christopher Davies Penicillin-binding proteins (PBPs), which are the lethal targets of β-lactam antibiotics, catalyse the final stages of peptidoglycan biosynthesis of the bacterial cell wall. PBP 5 of Escherichia coli is a D -alanine CPase...
Articles
Folding pathway of the pyridoxal 5′-phosphate C-S lyase MalY from Escherichia coli
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Journal:
Biochemical Journal
Biochem J (2005) 389 (3): 885–898.
Published: 26 July 2005
...Mariarita Bertoldi; Barbara Cellini; Douglas V. Laurents; Carla Borri Voltattorni MalY from Escherichia coli is a bifunctional dimeric PLP (pyridoxal 5′-phosphate) enzyme acting as a β-cystathionase and as a repressor of the maltose system. The spectroscopic and molecular properties...
Articles
Pseudomonas aeruginosa MutL protein functions in Escherichia coli
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Journal:
Biochemical Journal
Biochem J (2005) 388 (3): 879–887.
Published: 07 June 2005
...Daniela K. JACQUELÍN; Adrián FILIBERTI; Carlos E. ARGARAÑA; José L. BARRA Escherichia coli MutS, MutL and MutH proteins act sequentially in the MMRS (mismatch repair system). MutH directs the repair system to the newly synthesized strand due to its transient lack of Dam (DNA-adenine methylase...
Articles
Positive co-operative activity and dimerization of the isolated ABC ATPase domain of HlyB from Escherichia coli
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Journal:
Biochemical Journal
Biochem J (2005) 386 (3): 489–495.
Published: 08 March 2005
...Houssain BENABDELHAK; Lutz SCHMITT; Carsten HORN; Kornelia JUMEL; Mark A. BLIGHT; I. Barry HOLLAND The ATPase activity of the ABC (ATP-binding cassette) ATPase domain of the HlyB (haemolysin B) transporter is required for secretion of Escherichia coli haemolysin via the type I pathway. Although ABC...
Articles
Probing the active site of YjeE: a vital Escherichia coli protein of unknown function
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Journal:
Biochemical Journal
Biochem J (2004) 384 (3): 577–584.
Published: 07 December 2004
...Abdellah ALLALI-HASSANI; Tracey L. CAMPBELL; Andy HO; Jeffrey W. SCHERTZER; Eric D. BROWN In the study described here, we have taken steps to characterize the YjeE protein, an Escherichia coli protein of unknown function that is essential for bacterial viability. YjeE represents a protein family...
Articles
The strong efficiency of the Escherichia coli gapA P1 promoter depends on a complex combination of functional determinants
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Journal:
Biochemical Journal
Biochem J (2004) 383 (2): 371–382.
Published: 08 October 2004
...Benoit THOUVENOT; Bruno CHARPENTIER; Christiane BRANLANT The Escherichia coli multi-promoter region of the gapA gene ensures a high level of GAPDH (glyceraldehyde-3-phosphate dehydrogenase) production under various growth conditions. In the exponential phase of growth, gapA mRNAs are mainly...
Articles
Effects of the antimicrobial peptide temporin L on cell morphology, membrane permeability and viability of Escherichia coli
Available to PurchaseMaria Luisa MANGONI, Niv PAPO, Donatella BARRA, Maurizio SIMMACO, Argante BOZZI, Antonio DI GIULIO, Andrea C. RINALDI
Journal:
Biochemical Journal
Biochem J (2004) 380 (3): 859–865.
Published: 15 June 2004
... of evidence that many of them interact and increase the permeability of bacterial membranes as part of their killing mechanism. However, it is not clear whether this is the lethal step. To address this issue, we studied the interaction of the antimicrobial peptide temporin L with Escherichia coli by using...
Articles
Overexpression of UDP-glucose dehydrogenase in Escherichia coli results in decreased biosynthesis of K5 polysaccharide
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Journal:
Biochemical Journal
Biochem J (2003) 374 (3): 767–772.
Published: 15 September 2003
...Elisabet ROMAN; Ian ROBERTS; Kerstin LIDHOLT; Marion KUSCHE-GULLBERG The Escherichia coli K5 capsular polysaccharide (glycosaminoglycan) chains are composed of the repeated disaccharide structure: -GlcAβ1,4-GlcNAcα1,4-(where GlcA is glucuronic acid and GlcNAc is N -acetyl- d -glucosamine). The GlcA...
Articles
Degradation of mutant initiator protein DnaA204 by proteases ClpP, ClpQ and Lon is prevented when DNA is SeqA-free
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Journal:
Biochemical Journal
Biochem J (2003) 370 (3): 867–871.
Published: 15 March 2003
...Monika SLOMINSKA; Anne WAHL; Grzegorz WEGRZYN; Kirsten SKARSTAD A mutant form of the Escherichia coli replication initiator protein, DnaA204, is unstable. At low growth rates, the dnaA204 mutant cells experience a limitation of initiator protein and grow with reduced initiation frequency and DNA...
Articles
Cloning of thioredoxin h reductase and characterization of the thioredoxin reductase–thioredoxin h system from wheat
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Journal:
Biochemical Journal
Biochem J (2002) 367 (2): 491–497.
Published: 15 October 2002
... site of thioredoxin h. Wheat NTR was expressed in Escherichia coli as a His-tagged protein. The absorption spectrum of the purified recombinant protein is typical of flavoenzymes. Furthermore, it showed NADPH-dependent thioredoxin h re- INTRODUCTION Thioredoxins are ubiquitous proteins with molecular...
Articles
Biochemical analysis of DnaA protein with mutations in both Arg328 and Lys372
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Journal:
Biochemical Journal
Biochem J (2002) 362 (2): 453–458.
Published: 22 February 2002
...Masaki MAKISE; Shinji MIMA; Motohiro KOTERASAWA; Tomofusa TSUCHIYA; Tohru MIZUSHIMA The DnaA protein is the initiator of chromosomal DNA replication in Escherichia coli . Acidic phospholipids decrease its affinity for adenine nucleotides, and re-activate the ADP-bound form to the ATP-bound form. We...
Articles
Local and spatial factors determining HIV-1 protease substrate recognition
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Journal:
Biochemical Journal
Biochem J (2001) 358 (2): 505–510.
Published: 24 August 2001
...Stephane HAZEBROUCK; Valerie MACHTELINCKX-DELMAS; Jean-Jacques KUPIEC; Pierre SONIGO Insertional mutagenesis of the Escherichia coli thymidylate synthase (TS) was used to address substrate recognition of HIV-1 protease in a well characterized structural context. By modifying the TS conformation...
Articles
Continuous affinity-based selection: rapid screening and simultaneous amplification of bacterial surface-display libraries
Available to PurchaseDipak PATEL, Srdjan VITOVSKI, H. Jennifer SENIOR, Michael D. EDGE, Robert C. HOCKNEY, Michael J. DEMPSEY, Jon R. SAYERS
Journal:
Biochemical Journal
Biochem J (2001) 357 (3): 779–785.
Published: 25 July 2001
..., Escherichia coli, lamB, phage lambda receptor, peptide library. the total pool of cells, but to enrich and amplify simultaneously for the isolated sub-populations. We wished to develop a continuous culture system for the rapid isolation}screening and amplification of cells expressing a valuable protein...
Articles
Suppression of temperature-sensitivity of a dnaA46 mutant by excessive DNA supercoiling
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Journal:
Biochemical Journal
Biochem J (2000) 348 (2): 375–379.
Published: 23 May 2000
[email protected]). 7 2 2000 10 3 2000 29 3 2000 The Biochemical Society, London © 2000 2000 DNA replication DnaA protein Escherichia coli linking number nalidixic acid Biochem. J. (2000) 348, 375 379 (Printed in Great Britain) 375 Suppression of temperature-sensitivity...
Articles
Deuterium-labelled isotopomers of 2-C-methyl-d-erythritol as tools for the elucidation of the 2-C-methyl-d-erythritol 4-phosphate pathway for isoprenoid biosynthesis
Available to PurchaseLionel CHARON, Jean-François HOEFFLER, Catherine PALE-GROSDEMANGE, Luisa-Maria LOIS, Narciso CAMPOS, Albert BORONAT, Michel ROHMER
Journal:
Biochemical Journal
Biochem J (2000) 346 (3): 737–742.
Published: 07 March 2000
...Lionel CHARON; Jean-François HOEFFLER; Catherine PALE-GROSDEMANGE; Luisa-Maria LOIS; Narciso CAMPOS; Albert BORONAT; Michel ROHMER Escherichia coli synthesizes its isoprenoids via the mevalonate-independent 2- C -methyl- D -erythritol 4-phosphate (MEP) pathway. The MC4100 dxs ::CAT strain...
Articles
2,4′-Dihydroxyacetophenone dioxygenase (EC 1.13.11.41) from Alcaligenes sp. 4HAP: a novel enzyme with an atypical dioxygenase sequence
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Journal:
Biochemical Journal
Biochem J (1999) 344 (2): 397–402.
Published: 24 November 1999
... sequence, confirms the N-terminal amino acid sequencing data and contains the sequence of an internal tryptic peptide. It gave a calculated M r of 20364. The gene was expressed in Escherichia coli and yielded active enzyme. The derived amino acid sequence does not show significant similarity to other...
Articles
Involvement of Arg-328, Arg-334 and Arg-342 of DnaA protein in the functional interaction with acidic phospholipids
Available to PurchaseYoshihiro YAMAGUCHI, Masakazu HASE, Masaki MAKISE, Shinji MIMA, Takeshi YOSHIMI, Yuichi ISHIKAWA, Tomofusa TSUCHIYA, Tohru MIZUSHIMA
Journal:
Biochemical Journal
Biochem J (1999) 340 (2): 433–438.
Published: 25 May 1999
[email protected]). 4 12 1998 3 2 1999 25 2 1999 The Biochemical Society, London © 1999 1999 basic amino acid Escherichia coli initiator protein membrane binding site-directed mutagenesis Biochem. J. (1999) 340, 433 438 (Printed in Great Britain) 433 Involvement of Arg...
