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A structure model explaining the binding between a ubiquitous unconventional G-protein (OsYchF1) and a plant-specific C2-domain protein (OsGAP1) from rice

Ming-Yan Cheung, Jacky Chi-Ki Ngo, Zhongzhou Chen, Qi Jia, Tianjie Li, Yitao Gou, Yi Wang, Hon-Ming Lam

Biochem J (2020) 477 (20): 3935–3949.

https://doi.org/10.1042/BCJ20200380

Published: 23 October 2020

...Ming-Yan Cheung; Jacky Chi-Ki Ngo; Zhongzhou Chen; Qi Jia; Tianjie Li; Yitao Gou; Yi Wang; Hon-Ming Lam The unconventional G-protein OsYchF1 plays regulatory roles in plant defense and abiotic stress responses. We have previously resolved the crystal structures of OsYchF1 and its plant-specific...

View article titled, A structure model explaining the binding between a ubiquitous unconventional G-protein (OsYchF1) and a plant-specific C2-domain protein (OsGAP1) from rice

Includes: Supplementary data

Articles

AZD8797 is an allosteric non-competitive modulator of the human CX3CR1 receptor

Linda Cederblad, Birgitta Rosengren, Erik Ryberg, Nils-Olov Hermansson

Biochem J (2016) 473 (5): 641–649.

https://doi.org/10.1042/BJ20150520

Published: 24 February 2016

...) and in a B-lymphocyte cell line with IC 50 values of 300 and 6 nM respectively. AZD8797 also prevented G-protein activation in a [ 35 S]GTPγS (guanosine 5′-[γ-thio]triphosphate) accumulation assay. In contrast, dynamic mass redistribution (DMR) experiments revealed a weak G αi -dependent AZD8797 agonism...

View article titled, AZD8797 is an allosteric non-competitive modulator of the human CX3CR1 receptor

Articles

Different inhibition of Gβγ-stimulated class IB phosphoinositide 3-kinase (PI3K) variants by a monoclonal antibody. Specific function of p101 as a Gβγ-dependent regulator of PI3Kγ enzymatic activity

Aliaksei Shymanets, Prajwal, Oscar Vadas, Cornelia Czupalla, Jaclyn LoPiccolo, Michael Brenowitz, Alessandra Ghigo, Emilio Hirsch, Eberhard Krause, Reinhard Wetzker, Roger L. Williams, Christian Harteneck, Bernd Nürnberg

Biochem J (2015) 469 (1): 59–69.

https://doi.org/10.1042/BJ20150099

Published: 19 June 2015

... enzymes downstream of signalling cascades triggered by G-protein-coupled receptors (GPCRs). PI3Kγ variants have one catalytic p110γ subunit that can form two different heterodimers by binding to one of a pair of non-catalytic subunits, p87 or p101. Growing experimental data argue for a different...

View article titled, Different inhibition of Gβγ-stimulated class IB phosphoinositide 3-kinase (PI3K) variants by a monoclonal antibody. Specific function of p101 as a Gβγ-dependent regulator of PI3Kγ enzymatic activity

Articles

The p101 subunit of PI3Kγ restores activation by Gβ mutants deficient in stimulating p110γ

Aliaksei Shymanets, Mohammad R. Ahmadian, Katja T. Kössmeier, Reinhard Wetzker, Christian Harteneck, Bernd Nürnberg

Biochem J (2012) 441 (3): 851–858.

https://doi.org/10.1042/BJ20111664

Published: 16 January 2012

...Aliaksei Shymanets; Mohammad R. Ahmadian; Katja T. Kössmeier; Reinhard Wetzker; Christian Harteneck; Bernd Nürnberg G-protein-regulated PI3Kγ (phosphoinositide 3-kinase γ) plays a crucial role in inflammatory and allergic processes. PI3Kγ, a dimeric protein formed by the non-catalytic p101...

View article titled, The p101 subunit of PI3Kγ restores activation by Gβ mutants deficient in stimulating p110γ

Articles

Substrate binding disrupts dimerization and induces nucleotide exchange of the chloroplast GTPase Toc33

Mislav Oreb, Anja Höfle, Patrick Koenig, Maik S. Sommer, Irmgard Sinning, Fei Wang, Ivo Tews, Danny J. Schnell, Enrico Schleiff

Biochem J (2011) 436 (2): 313–319.

https://doi.org/10.1042/BJ20110246

Published: 13 May 2011

.... dimeric GTPase GDP-dissociation-inhibitor function (GDI function) G-protein protein translocation substrate-based regulation 2 Present address: Department of Biosciences, Physiology and Genetics of Lower Eukaryotes, Goethe University Frankfurt, D-60438 Frankfurt am Main, Germany. 3...

View article titled, Substrate binding disrupts dimerization and induces nucleotide exchange of the chloroplast GTPase Toc33

Articles

Wnt signalling: the case of the ‘missing’ G-protein

Craig C. Malbon

Biochem J (2011) 433 (3): e3–e5.

https://doi.org/10.1042/BJ20102111

Published: 14 January 2011

...Craig C. Malbon Wnt signalling remains a hot topic for cell signalling sleuthhounds. The trail of signalling downstream of the seven-transmembrane segment Frizzleds, which bind Wnt ligands, is replete of clues [e.g. LPR5/6 (lipoprotein receptor-related protein 5/6), G-proteins or Dishevelled...

View article titled, Wnt signalling: the case of the ‘missing’ G-protein

Articles

The vasoactive peptides urotensin II and urotensin II-related peptide regulate astrocyte activity through common and distinct mechanisms: involvement in cell proliferation

Marie Jarry, Mickaël Diallo, Céline Lecointre, Laurence Desrues, Tursonjan Tokay, David Chatenet, Jérôme Leprince, Oriana Rossi, Hubert Vaudry, Marie-Christine Tonon, Laurent Prézeau, Hélène Castel, Pierrick Gandolfo

Biochem J (2010) 428 (1): 113–124.

https://doi.org/10.1042/BJ20090867

Published: 28 April 2010

.... astrocyte bioluminescence resonance energy transfer calcium cell proliferation G-protein urotensin II receptor Astrocytes (5 DIV) were incubated for 60 min at 37 °C with fresh serum-free medium in the absence or presence of test substances. Peptides contained in culture medium were concentrated...

View article titled, The vasoactive peptides urotensin II and urotensin II-related peptide regulate astrocyte activity through common and distinct mechanisms: involvement in cell proliferation

Articles

Interaction of retinal guanylate cyclase with the α subunit of transducin: potential role in transducin localization

Derek H. Rosenzweig, K. Saidas Nair, Konstantin Levay, Igor V. Peshenko, John W. Crabb, Alexander M. Dizhoor, Vladlen Z. Slepak

Biochem J (2009) 417 (3): 803–812.

https://doi.org/10.1042/BJ20081513

Published: 16 January 2009

... hydrolysis via the G-protein transducin, which directly binds to and activates phosphodiesterase. Bright light also causes relocalization of transducin from the OS (outer segments) of the rod cells to the inner compartments. In the present study, we show experimental evidence for a previously unknown...

View article titled, Interaction of retinal guanylate cyclase with the α subunit of transducin: potential role in transducin localization

Includes: Supplementary data

Articles

Pathways of signal transduction employed by vertebrate Hedgehogs

Natalia A. Riobo, David R. Manning

Biochem J (2007) 403 (3): 369–379.

https://doi.org/10.1042/BJ20061723

Published: 12 April 2007

...-proteins and G-protein-coupled receptor kinases by Smoothened. We also address the pathways, insofar as known, linking Smoothened to the expression and stability of Gli1, Gli2 and Gli3. The mechanisms by which Hh proteins signal have few, if any, parallels. It is becoming clear in vertebrates, however...

View article titled, Pathways of signal transduction employed by vertebrate Hedgehogs

Articles

Big roles for small GTPases in the control of directed cell movement

Pascale G. Charest, Richard A. Firtel

Biochem J (2007) 401 (2): 377–390.

https://doi.org/10.1042/BJ20061432

Published: 21 December 2006

... shed light on the molecular mechanisms by which small GTPases control directed cell migration. cell polarity cellular motility directional sensing eukaryotic chemotaxis G-protein The migration of neutrophils to sites of inflammation, the organization of embryonic cells during morphogenesis...

View article titled, Big roles for small GTPases in the control of directed cell movement

Articles

Regulation of the cardiac L-type calcium channel in L6 cells by arginine-vasopressin

Basil M. Hantash, Andrew P. Thomas, John P. Reeves

Biochem J (2006) 400 (3): 411–419.

https://doi.org/10.1042/BJ20060742

Published: 28 November 2006

... into the appropriate medium. This yielded a final DMSO or chloroform concentration of 0.1% or less, which alone had no effect on L-type Ca 2+ channel activity in our assay. In order to ADP-ribosylate all G-protein α subunits of the G i family, we treated L6 cells for 18–24 h with 100 ng/ml PTX. PTX was also...

View article titled, Regulation of the cardiac L-type calcium channel in L6 cells by arginine-vasopressin

Articles

Rapid stimulation of tyrosine phosphorylation signals downstream of G-protein-coupled receptors for thromboxane A 2 in human platelets

Pietro Minuz, Laura Fumagalli, Stefania Gaino, Rosa M. Tommasoli, Maurizio Degan, Chiara Cavallini, Anna Lecchi, Marco Cattaneo, Clara Lechi Santonastaso, Giorgio Berton

Biochem J (2006) 400 (1): 127–134.

https://doi.org/10.1042/BJ20061015

Published: 27 October 2006

...Pietro Minuz; Laura Fumagalli; Stefania Gaino; Rosa M. Tommasoli; Maurizio Degan; Chiara Cavallini; Anna Lecchi; Marco Cattaneo; Clara Lechi Santonastaso; Giorgio Berton Signals ensuing from trimeric G-protein-coupled receptors synergize to induce platelet activation. At low doses, the thromboxane...

View article titled, Rapid stimulation of tyrosine phosphorylation signals downstream of G-protein-coupled receptors for thromboxane A 2 in human platelets

Articles

The allosteric enhancer PD81,723 increases chimaeric A 1 /A 2A adenosine receptor coupling with G s

Samita Bhattacharya, Rebecca L. Youkey, Kobina Ghartey, Matthew Leonard, Joel Linden, Amy L. Tucker

Biochem J (2006) 396 (1): 139–146.

https://doi.org/10.1042/BJ20051422

Published: 26 April 2006

... -coupled A 2A ARs. PD81,723 elicits a decrease in the dissociation kinetics of A 1 AR agonist radioligands and an increase in functional agonist potency. In the present study, we sought to determine whether enhancer sensitivity is dependent on coupling domains or G-protein specificity of the A 1 AR. Using...

View article titled, The allosteric enhancer PD81,723 increases chimaeric A 1 /A 2A adenosine receptor coupling with G s

Articles

New insights into the families of PLC enzymes: looking back and going forward

Matilda Katan

Biochem J (2005) 391 (3): e7.

https://doi.org/10.1042/BJ20051506

Published: 25 October 2005

... (phosphoinositide-specific phospholipase C) enzymes, PLCη. Two isoforms, PLCη1 and PLCη2, and their splice variants add to the molecular diversity of PLC enzymes. The studies of PLCη regulation suggest that at least some splice variants of PLCη2 could be regulated by the G-protein subunits Gβγ. As two other...

View article titled, New insights into the families of PLC enzymes: looking back and going forward

Articles

Molecular cloning and characterization of PLC-η2

Yixing Zhou, Michele R. Wing, John Sondek, T. Kendall Harden

Biochem J (2005) 391 (3): 667–676.

https://doi.org/10.1042/BJ20050839

Published: 25 October 2005

... spliced first exons. Co-expression of PLC-η2 with Gβ 1 γ 2 dimers of heterotrimeric G-proteins resulted in marked stimulation of inositol lipid hydrolysis. Thus PLC-η2 may in part function downstream of G-protein-coupled receptors. 1 To whom correspondence should be addressed (email tkh...

View article titled, Molecular cloning and characterization of PLC-η2

Articles

Phosphatidylinositol 3,4,5-trisphosphate and Ca 2+ /calmodulin competitively bind to the regulators of G-protein-signalling (RGS) domain of RGS4 and reciprocally regulate its action

Masaru ISHII, Satoru FUJITA, Mitsuhiko YAMADA, Yukio HOSAKA, Yoshihisa KURACHI

Biochem J (2005) 385 (1): 65–73.

https://doi.org/10.1042/BJ20040404

Published: 14 December 2004

...Masaru ISHII; Satoru FUJITA; Mitsuhiko YAMADA; Yukio HOSAKA; Yoshihisa KURACHI RGS (regulators of G-protein signalling) are a diverse group of proteins, which accelerate intrinsic GTP hydrolysis on heterotrimeric G-protein α subunits. They are involved in the control of a physiological behaviour...

View article titled, Phosphatidylinositol 3,4,5-trisphosphate and Ca 2+ /calmodulin competitively bind to the regulators of G-protein-signalling (RGS) domain of RGS4 and reciprocally regulate its action

Articles

Inositol phospholipids regulate the guanine-nucleotide-exchange factor Tiam1 by facilitating its binding to the plasma membrane and regulating GDP/GTP exchange on Rac1

Ian N. FLEMING, Ian H. BATTY, Alan R. PRESCOTT, Alex GRAY, Gursant S. KULAR, Hazel STEWART, C. Peter DOWNES

Biochem J (2004) 382 (3): 857–865.

https://doi.org/10.1042/BJ20040916

Published: 07 September 2004

[email protected] ). 1 6 2004 2 7 2004 9 7 2004 9 7 2004 The Biochemical Society, London 2004 G-protein guanine-nucleotide-exchange factor inositol phospholipid pleckstrin homology domain Rac1 Tiam1 PH (pleckstrin homology) domains are poorly conserved protein modules...

View article titled, Inositol phospholipids regulate the guanine-nucleotide-exchange factor Tiam1 by facilitating its binding to the plasma membrane and regulating GDP/GTP exchange on Rac1

Articles

Ligand-dependent autophosphorylation of the insulin receptor is positively regulated by Gi-proteins

J. KREUZER, B. NÜRNBERG, H. I. KRIEGER-BRAUER

Biochem J (2004) 380 (3): 831–836.

https://doi.org/10.1042/bj20031659

Published: 15 June 2004

... function. For the experiments, isolated IRs from plasma membranes of human fat cells were used. The activation of IR autophosphorylation by insulin was blocked by G-protein inactivation through GDPβS (guanosine 5´-[β-thio]disphosphate). Consistently, activation of G-proteins by micromolar concentrations...

View article titled, Ligand-dependent autophosphorylation of the insulin receptor is positively regulated by Gi-proteins

Includes: Supplementary data

Articles

Identification of a stretch of six divergent amino acids on the alpha5 helix of Galpha16 as a major determinant of the promiscuity and efficiency of receptor coupling

Maurice K. C. HO, Jasmine H. P. CHAN, Cecilia S. S. WONG, Yung H. WONG

Biochem J (2004) 380 (2): 361–369.

https://doi.org/10.1042/bj20040231

Published: 01 June 2004

...Maurice K. C. HO; Jasmine H. P. CHAN; Cecilia S. S. WONG; Yung H. WONG A broad repertory of G-protein-coupled receptors shows effective coupling with the haematopoietic G 16 protein. In the present study, individual residues along the C-terminal α5 helix of Gα 16 were examined...

View article titled, Identification of a stretch of six divergent amino acids on the alpha5 helix of Galpha16 as a major determinant of the promiscuity and efficiency of receptor coupling

Articles

Mutation of cysteine 214 in Gi1 alpha subunit abolishes its endogenous GTPase activity

Yuren WANG, Gregory TAWA, Deborah SMITH, Girija KRISHNAMURTHY, Kathleen H. YOUNG

Biochem J (2004) 379 (3): 673–679.

https://doi.org/10.1042/bj20031343

Published: 01 May 2004

...-mail [email protected] ). 3 9 2003 5 1 2004 16 1 2004 16 1 2004 The Biochemical Society, London ©2004 2004 cysteine G-protein GTPase GTP hydrolysis site-directed mutagenesis Abbreviations used: GST, glutathione S-transferase; CHO, Chinese hamster ovary; GTP[S...

View article titled, Mutation of cysteine 214 in Gi1 alpha subunit abolishes its endogenous GTPase activity

Articles

Purification and biochemical characterization of the D6 chemokine receptor

Paul E. BLACKBURN, Clare V. SIMPSON, Robert J. B. NIBBS, Maureen O'HARA, Rhona BOOTH, Jemma POULOS, Neil W. ISAACS, Gerard J. GRAHAM

Biochem J (2004) 379 (2): 263–272.

https://doi.org/10.1042/bj20031266

Published: 15 April 2004

... here, leads the way towards work aimed at generating the three-dimensional structure of this seven-transmembrane- spanning receptor. Key words: chemokine, D6 chemokine receptor, G-protein, purification, structure. INTRODUCTION Chemokines are typically defined as regulators of the migration of cells...

View article titled, Purification and biochemical characterization of the D6 chemokine receptor

Articles

Role of receptor tyrosine kinases in G-protein-coupled receptor regulation of Ras: transactivation or parallel pathways?

Julian DOWNWARD

Biochem J (2003) 376 (1): e9–e10.

https://doi.org/10.1042/bj20031745

Published: 15 November 2003

...Julian DOWNWARD Ras protein regulation by G-protein-coupled receptors has been thought to occur through transactivation of receptor tyrosine kinases. New evidence suggests that these two receptor types independently control different pathways leading to Ras activation in response...

View article titled, Role of receptor tyrosine kinases in G-protein-coupled receptor regulation of Ras: transactivation or parallel pathways?

Articles

Co-incident signalling between μ-opioid and M3 muscarinic receptors at the level of Ca2+ release from intracellular stores: lack of evidence for Ins(1,4,5)P3 receptor sensitization

Damien S. K. SAMWAYS, Wen-hong LI, Stuart J. CONWAY, Andrew B. HOLMES, Martin D. BOOTMAN, Graeme HENDERSON

Biochem J (2003) 375 (3): 713–720.

https://doi.org/10.1042/bj20030508

Published: 01 November 2003

... 2003 24 7 2003 25 7 2003 25 7 2003 The Biochemical Society, London ©2003 2003 Ca 2+ G-protein Ins P 3 μ-opioid Abbreviations used: AM, acetoxymethyl ester; [Ca 2+ ] i , intracellular free-Ca 2+ concentration; Ins P 3 , Ins(1,4,5) P 3 ; ciIns P 3 /PM, caged...

View article titled, Co-incident signalling between μ-opioid and M3 muscarinic receptors at the level of Ca2+ release from intracellular stores: lack of evidence for Ins(1,4,5)P3 receptor sensitization

Articles

Regulation and organization of adenylyl cyclases and cAMP

Dermot M. F. COOPER

Biochem J (2003) 375 (3): 517–529.

https://doi.org/10.1042/bj20031061

Published: 01 November 2003

... cAMP fluctuations in these various compartments. 1 e-mail [email protected] 15 7 2003 7 8 2003 26 8 2003 26 8 2003 The Biochemical Society, London ©2003 2003 adenylyl cyclase calcium cAMP G-protein protein kinase A (PKA) raft Abbreviations used: AC...

View article titled, Regulation and organization of adenylyl cyclases and cAMP

Articles

Activation of phosphoinositide 3-kinase by D2 receptor prevents apoptosis in dopaminergic cell lines

Venugopalan D. NAIR, C. Warren OLANOW, Stuart C. SEALFON

Biochem J (2003) 373 (1): 25–32.

https://doi.org/10.1042/bj20030017

Published: 01 July 2003

... do not express detectable D 2 receptors. Notably, the agonists studied differ in their relative efficacy to mediate anti-apoptotic effects and in their capacity to stimulate [ 35 S]guanosine 5′-[γ-thio]triphosphate ([ 35 S]GTP[S]) binding, an indicator of G-protein activation. Studies with inhibitors...

View article titled, Activation of phosphoinositide 3-kinase by D2 receptor prevents apoptosis in dopaminergic cell lines

Articles

RGS3 interacts with 14-3-3 via the N-terminal region distinct from the RGS (regulator of G-protein signalling) domain

Jiaxin NIU, Astrid SCHESCHONKA, Kirk M. DRUEY, Amanda DAVIS, Eleanor REED, Vladimir KOLENKO, Richard BODNAR, Tatyana VOYNO-YASENETSKAYA, Xiaoping DU, John KEHRL, Nickolai O. DULIN

Biochem J (2002) 365 (3): 677–684.

https://doi.org/10.1042/bj20020390

Published: 01 August 2002

...Jiaxin NIU; Astrid SCHESCHONKA; Kirk M. DRUEY; Amanda DAVIS; Eleanor REED; Vladimir KOLENKO; Richard BODNAR; Tatyana VOYNO-YASENETSKAYA; Xiaoping DU; John KEHRL; Nickolai O. DULIN RGS3 belongs to a family of the regulators of G-protein signalling (RGS), which bind and inhibit the Gα subunits...

View article titled, RGS3 interacts with 14-3-3 via the N-terminal region distinct from the RGS (regulator of G-protein signalling) domain

Articles

Homo- and hetero-oligomeric interactions between G-protein-coupled receptors in living cells monitored by two variants of bioluminescence resonance energy transfer (BRET): hetero-oligomers between receptor subtypes form more efficiently than between less closely related sequences

Douglas RAMSAY, Elaine KELLETT, Mary McVEY, Stephen REES, Graeme MILLIGAN

Biochem J (2002) 365 (2): 429–440.

https://doi.org/10.1042/bj20020251

Published: 15 July 2002

...Douglas RAMSAY; Elaine KELLETT; Mary McVEY; Stephen REES; Graeme MILLIGAN Homo- and hetero-oligomerization of G-protein-coupled receptors (GPCRs) were examined in HEK-293 cells using two variants of bioluminescence resonance energy transfer (BRET). BRET 2 (a variant of BRET) offers greatly improved...

View article titled, Homo- and hetero-oligomeric interactions between G-protein-coupled receptors in living cells monitored by two variants of bioluminescence resonance energy transfer (BRET): hetero-oligomers between receptor subtypes form more efficiently than between less closely related sequences

Articles

Suramin and the suramin analogue NF307 discriminate among calmodulin-binding sites

Markus KLINGER, Elisa BOFILL-CARDONA, Bernd MAYER, Christian NANOFF, Michael FREISSMUTH, Martin HOHENEGGER

Biochem J (2001) 355 (3): 827–833.

https://doi.org/10.1042/bj3550827

Published: 24 April 2001

... were purified [ryanodine receptor-1 (RyR1) from rabbit skeletal muscle, neuronal NO synthase (nNOS) from Sf9 cells, G-protein βγ dimers (Gβγ) from porcine brain and a glutathione S-transferase-fusion protein comprising the C-terminal calmodulin-binding domain of the metabotropic glutamate receptor 7A...

View article titled, Suramin and the suramin analogue NF307 discriminate among calmodulin-binding sites

Articles

Peptide YY Y1 receptor activates mitogen-activated protein kinase and proliferation in gut epithelial cells via the epidermal growth factor receptor

Peter J. MANNON, Jennifer M. MELE

Biochem J (2000) 350 (3): 655–661.

https://doi.org/10.1042/bj3500655

Published: 08 September 2000

...Peter J. MANNON; Jennifer M. MELE The G-protein-coupled peptide YY (PYY)/neuropeptide Y Y1 receptor (Y1R) subtype is highly expressed in the proliferative zone of human colonic crypt epithelial cells but biochemical and biological support for growth effects have been lacking. Using a model gut...

View article titled, Peptide YY Y1 receptor activates mitogen-activated protein kinase and proliferation in gut epithelial cells via the epidermal growth factor receptor

Articles

Palmitoylation of the recombinant human A1 adenosine receptor: enhanced proteolysis of palmitoylation-deficient mutant receptors

Zhenhai GAO, Yajun NI, Gabor SZABO, Joel LINDEN

Biochem J (1999) 342 (2): 387–395.

https://doi.org/10.1042/bj3420387

Published: 24 August 1999

...), down-regulation (24 h), inhibition of forskolin-stimulated cAMP accumulation, or activation of co-transfected G-protein-activated inward rectifier K + /cardiac inward rectifying K + (GIRK1/CIR K + ) channels. Also unaffected by palmitoylation is guanosine 5′-[γ-thio]-triphosphate ([S]GTP)-sensitive...

View article titled, Palmitoylation of the recombinant human A1 adenosine receptor: enhanced proteolysis of palmitoylation-deficient mutant receptors

NARROW

A structure model explaining the binding between a ubiquitous unconventional G-protein (OsYchF1) and a plant-specific C2-domain protein (OsGAP1) from rice

AZD8797 is an allosteric non-competitive modulator of the human CX3CR1 receptor

Different inhibition of Gβγ-stimulated class IB phosphoinositide 3-kinase (PI3K) variants by a monoclonal antibody. Specific function of p101 as a Gβγ-dependent regulator of PI3Kγ enzymatic activity

The p101 subunit of PI3Kγ restores activation by Gβ mutants deficient in stimulating p110γ

Substrate binding disrupts dimerization and induces nucleotide exchange of the chloroplast GTPase Toc33

Wnt signalling: the case of the ‘missing’ G-protein

The vasoactive peptides urotensin II and urotensin II-related peptide regulate astrocyte activity through common and distinct mechanisms: involvement in cell proliferation

Interaction of retinal guanylate cyclase with the α subunit of transducin: potential role in transducin localization

Pathways of signal transduction employed by vertebrate Hedgehogs

Big roles for small GTPases in the control of directed cell movement

Regulation of the cardiac L-type calcium channel in L6 cells by arginine-vasopressin

Rapid stimulation of tyrosine phosphorylation signals downstream of G-protein-coupled receptors for thromboxane A 2 in human platelets

The allosteric enhancer PD81,723 increases chimaeric A 1 /A 2A adenosine receptor coupling with G s

New insights into the families of PLC enzymes: looking back and going forward

Molecular cloning and characterization of PLC-η2

Phosphatidylinositol 3,4,5-trisphosphate and Ca 2+ /calmodulin competitively bind to the regulators of G-protein-signalling (RGS) domain of RGS4 and reciprocally regulate its action

Inositol phospholipids regulate the guanine-nucleotide-exchange factor Tiam1 by facilitating its binding to the plasma membrane and regulating GDP/GTP exchange on Rac1

Ligand-dependent autophosphorylation of the insulin receptor is positively regulated by Gi-proteins

Identification of a stretch of six divergent amino acids on the alpha5 helix of Galpha16 as a major determinant of the promiscuity and efficiency of receptor coupling

Mutation of cysteine 214 in Gi1 alpha subunit abolishes its endogenous GTPase activity

Purification and biochemical characterization of the D6 chemokine receptor

Role of receptor tyrosine kinases in G-protein-coupled receptor regulation of Ras: transactivation or parallel pathways?

Co-incident signalling between μ-opioid and M3 muscarinic receptors at the level of Ca2+ release from intracellular stores: lack of evidence for Ins(1,4,5)P3 receptor sensitization

Regulation and organization of adenylyl cyclases and cAMP

Activation of phosphoinositide 3-kinase by D2 receptor prevents apoptosis in dopaminergic cell lines

RGS3 interacts with 14-3-3 via the N-terminal region distinct from the RGS (regulator of G-protein signalling) domain

Homo- and hetero-oligomeric interactions between G-protein-coupled receptors in living cells monitored by two variants of bioluminescence resonance energy transfer (BRET): hetero-oligomers between receptor subtypes form more efficiently than between less closely related sequences

Suramin and the suramin analogue NF307 discriminate among calmodulin-binding sites

Peptide YY Y1 receptor activates mitogen-activated protein kinase and proliferation in gut epithelial cells via the epidermal growth factor receptor

Palmitoylation of the recombinant human A1 adenosine receptor: enhanced proteolysis of palmitoylation-deficient mutant receptors

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