... standard buffer [25 mM Tris, 300 mM NaCl, 5 mM MgCl 2 , 5 mM 2-mercaptoethanol and 5% (v/v) glycerol, pH 7.8]. calorimetry crystal structure GTP hydrolysis septin SEPT3 Septins are guanine-nucleotide-binding proteins that were initially recognized as essential for the completion...

... compilation © 2009 Biochemical Society 2009 cytokinesis GTP hydrolysis microtubule OTBA (3-{5-[4-oxo-2-thioxo-3-(3-trifluoromethyl-phenyl)-thiazolidin-5-ylidenemethyl]-furan-2-yl}-benzoic acid) protofilament Z-ring A conventional method of drug discovery has been to search for antibiotics from...

... of the isothermal curve yielded a molar ratio of 0.87 and a K d value of 21 μM. The GTP-hydrolysis reaction is a fundamental process in living cells and represents an important timer in intracellular processes. The rate of Rho protein-mediated GTP hydrolysis is intrinsically low. Direct interaction...

... to function as Arf effectors and oncogenes. We have set out to characterize the kinetics of the GAP-induced GTP hydrolysis using a truncated form of ASAP1 [Arf GAP with SH3 (Src homology 3) domain, ankyrin repeats and PH (pleckstrin homology) domains 1] as a model. We found that ASAP1 used Arf1-GTP...

...-mail [email protected] ). 3 9 2003 5 1 2004 16 1 2004 16 1 2004 The Biochemical Society, London ©2004 2004 cysteine G-protein GTPase GTP hydrolysis site-directed mutagenesis Abbreviations used: GST, glutathione S-transferase; CHO, Chinese hamster ovary; GTP[S...

... functional significance for the phosphate-binding loop of Rab5 and shows that mutations in this region lead to either a loss-of-function or a gain-of-function phenotype, indicating a structure–function relationship distinct from that of Ras. endocytosis GTP binding GTP hydrolysis Ras structurefunction...

...Zhimin LIANG; Timothy MATHER; Guangpu LI Structural and biochemical data indicate the importance of the phosphate-binding loop residues Gly 12 and Gly 13 of Ras both in the GTP hydrolysis reaction and in biological activity, but these two residues are not conserved in other Ras-related GTPases...