... recessive Parkinson's disease (PD) whilst gain of function mutations in the LRRK2 kinase cause autosomal dominant PD. PINK1 indirectly regulates the phosphorylation of a subset of Rab GTPases at a conserved Serine111 (Ser111) residue within the SF3 motif. Using genetic code expansion technologies, we have...

...Larissa Legewie; Jennifer Loschwitz; Nora Steffens; Martin Prescher; Xue Wang; Sander H. J. Smits; Lutz Schmitt; Birgit Strodel; Daniel Degrandi; Klaus Pfeffer Guanylate-binding proteins (GBPs) constitute a family of interferon-inducible guanosine triphosphatases (GTPases) that are key players...

... cofilin and small GTPases, thereby linking EFhd2, synapses and the actin cytoskeleton. Expression data and functional experiments in mice and in humans have led to the hypothesis that down-regulation of EFhd2, especially in the cortex, is involved in dementia. 26 2 2016 20 4 2016 3 5 2016...

... GTPase kinase LRRK2 homologue neurodegeneration preclinical research The cause of most cases of PD is not known, leading to the description as a sporadic or idiopathic disease. However, in recent years it has been increasingly appreciated that monogenic forms of PD exist and that cloning...

...Kamal R. Abdul Azeez; Stefan Knapp; João M. P. Fernandes; Enno Klussmann; Jonathan M. Elkins The RhoGEF (Rho GTPase guanine-nucleotide-exchange factor) domain of AKAP-Lbc (A-kinase-anchoring protein-Lbc, also known as AKAP13) catalyses nucleotide exchange on RhoA and is involved in the development...

... GTPases regulates a host of cellular processes, including proliferation, differentiation and survival. This protein family functions as a molecular switch, transitioning from an inactive GDP-bound to an active GTP-bound state when triggered by signalling from receptor tyrosine kinases via the activation...

..., there is great interest in the identification of small-molecule inhibitors of RhoGTPases. In the present paper, using the property of GTP-loaded RhoGTPases to bind to their effectors, we describe a miniaturized and robust assay to monitor Rac1 GTPase activation that is suitable for large-scale high-throughput...

... was not significantly different from the wild-type upon non-stress microaerobic incubation of strains. Urease maturation occurs through nickel mobilization via a suite of known accessory proteins, one being the GTPase UreG. Treatment of UreG with H 2 O 2 resulted in oxidation of MS-identified methionine residues...

..., tetramethylrhodamine–ParM, can also monitor GDP. The biosensor can be used to measure micromolar concentrations of GDP on the background of millimolar concentrations of GTP. The fluorescence response of the biosensor is fast, the response time being <0.2 s. Thus the biosensor allows real-time measurements of GTPase...

...James P. Madigan; Brian O. Bodemann; Donita C. Brady; Brian J. Dewar; Patricia J. Keller; Michael Leitges; Mark R. Philips; Anne J. Ridley; Channing J. Der; Adrienne D. Cox The Rnd proteins (Rnd1, Rnd2 and Rnd3/RhoE) form a distinct branch of the Rho family of small GTPases. Altered Rnd3 expression...

...Tracey L. Campbell; Denis M. Daigle; Eric D. Brown We present an analysis of the cellular phenotype and biochemical activity of a conserved bacterial GTPase of unknown function (YloQ and YjeQ in Bacillus subtilis and Escherichia coli respectively) using a collection of antibiotics of diverse...

...-blot analysis revealed that RLP was strongly expressed in heart, brain and kidney, and below the detection level in spleen and skeletal muscles. At the protein level, RLP is approx. 30% homologous with members of the Ras superfamily, particularly in domains characteristic for small GTPases. However...

...Sabine TRAVER; Anne SPLINGARD; Georges GAUDRIAULT; Jean de GUNZBURG RGS (regulator of G-protein signalling) proteins stimulate the intrinsic GTPase activity of the α subunits of heterotrimeric G-proteins, and thereby negatively regulate G-protein-coupled receptor signalling. RGS14 has been shown...

... GTPase activity. Free phosphate release from [ 32 P]GTP-bound Gα i1 C214A or [ 32 P]GTP-bound Gα i1 C214W was at least 30-fold lower than that of the wild-type Gα i1 in single-turnover GTPase assays. Consistently, tryptic proteolysis of C214A and C214W proteins showed that they were partially protected...

...Pontus ASPENSTRÖM; Åsa FRANSSON; Jan SARAS The Rho GTPases are related to the Ras proto-oncogenes and consist of 22 family members. These proteins have important roles in regulating the organization of the actin filament system, and thereby the morphogenesis of vertebrate cells as well...

... and interacts with GTPase-activating protein in a manner indistinguishable from wild-type Cdc42Hs. Immunofluorescence localization studies revealed that this RhoGDI-binding-defective mutant is found predominantly in the Golgi apparatus, with a staining pattern similar to that of the wild-type protein. However...

... sequestration. G alpha interacting protein (GAIP),which stimulates the GTPase activity of the G αi3 protein and favours macroautophagic sequestration in HT-29 cells,was shown, by immunofluorescence studies using confocal microscopy, to be confined to the cytoplasm. The cytoplasmic distribution of GAIP only...