... at the cytoplasmic tail and the hydrophobic amino acids VIL (Val-Ile-Leu) in the outer part of the TMR (transmembrane region), lead to reduced raft association. In addition, exchange of VIL, but not of the S-acylation sites severely retards transport of HA through the Golgi. In the present study, we have further...

...(s) of GCKIIIs (and CCM3/PDCD10) in STRIPAK (striatin-interacting phosphatase and kinase) complexes and their association with the cis -Golgi protein GOLGA2 (golgin A2; GM130). Recently, an interaction of GCKIIIs with MO25 has been identified. This exhibits similarities to the STRADα (STE20-related...

.... In the present study, we show that a highly conserved Golgi-localized protein named HID-1 acts to prevent mis-sorting of peptide cargoes to lysosomes for degradation via a PtdIns3 P -dependent trafficking pathway. Epistasis analysis suggests that rab-2 is epistatic to hid-1 . 1 These authors contributed...

...Gerald R. V. Hammond; Giampietro Schiavo; Robin F. Irvine PtdIns4 P is the major precursor for the synthesis of the multifunctional plasma membrane lipid, PtdIns(4,5) P 2 . Yet PtdIns4 P also functions as a regulatory lipid in its own right, particularly at the Golgi apparatus. In the present study...

...Frøy Grøndahl; Heidi Tveit; Kristian Prydz PGs (proteoglycans) are proteins acquiring long, linear and sulfated GAG (glycosaminoglycan) chains during Golgi passage. In MDCK cells (Madin–Darby canine kidney cells), most of the CS (chondroitin sulfate) PGs are secreted apically, whereas most...

... benzaldehyde} has been reported to disrupt the Golgi apparatus completely and to stimulate Golgi–ER (endoplasmic reticulum) fusion in mammalian cells, akin to the well-characterized fungal toxin BFA (brefeldin A). It has also been reported that Exo2 does not affect the integrity of the TGN ( trans -Golgi...

... microscopy and subcellular fractionation, that modulation of SMS1 and, to a lesser extent, SMS2 affected the formation of DAG at the Golgi apparatus. Similarly, down-regulation of SMS1 and SMS2 reduced the localization of the DAG-binding protein PKD (protein kinase D) to the Golgi. These results provide...

... to characterize the localization and dynamics of ACBP in living cells. Results showed that ACBP targeted to the ER (endoplasmic reticulum) and Golgi in a ligand-binding-dependent manner. A variant Y28F/K32A-FACI-50, which is unable to bind acyl-CoA, did no longer show association with the ER and became segregated...

...Alberto M. Díaz Añel The requirement of DAG (diacylglycerol) to recruit PKD (protein kinase D) to the TGN ( trans -Golgi network) for the targeting of transport carriers to the cell surface, has led us to a search for new components involved in this regulatory pathway. Previous findings reveal...

... reported that Ser 262 present in the C-terminus of PITPβ is constitutively phosphorylated and determines Golgi localization. We provide evidence for the expression of an sp (splice) variant of PITPβ (PITPβ-sp2) where the C-terminal 15 amino acids of PITPβ-sp1 are replaced by an alternative C-terminus of 16...

... splice variant-EGFP or transporter-V5 expression plasmids along with organelle marker plasmids into HEK-293 cells (human embryonic kidney 293 cells) revealed that both rAbca5 and splice variant fusion proteins co-localized with marker protein for the Golgi apparatus. Expression of rAbca5 mRNA in Leydig...

.... Current data imply that the yeast ORP Kes1p is a negative regulator of Golgi-derived vesicular transport mediated by the essential phosphatidylinositol/phosphatidylcholine transfer protein Sec14p. Inactivation of Kes1p function allows restoration of growth and vesicular transport in cells lacking Sec14p...

... associated with intracellular organelles and, particularly, mitochondria. GSL biosynthesis starts with the formation of ceramide in the endoplasmic reticulum (ER), which is transported by controversial mechanisms to the Golgi apparatus, where stepwise addition of monosaccharides on to ceramides takes place...

...Svein Olav KOLSET; Kristian PRYDZ; Katja FJELDSTAD; Fariba SAFAIYAN; Tram Thu VUONG; Eva GOTTFRIDSSON; Markku SALMIVIRTA Brefeldin A (BFA) perturbs the organization of the Golgi apparatus, such that Golgi stack components are fused with the endoplasmic reticulum (ER) and separated from the trans...

...Abdelkarim ABOUSALHAM; Tom C. HOBMAN; Jay DEWALD; Michael GARBUTT; David N. BRINDLEY Differential effects of acetyl(C 2 -) ceramide ( N -acetylsphingosine) were studied on coated vesicle formation from Golgi-enriched membranes of Chinese hamster ovary (CHO) and Madin—Darby canine kidney (MDCK...

...Vítor L. SOUSA; Maria Teresa COSTA; Angelina S. PALMA; Francisco ENGUITA; Júlia COSTA Fucosyltransferase III [galactoside 3(4)- l -fucosyltransferase; EC 2.4.1.65] (FT3) is a Golgi type II membrane protein that catalyses the synthesis of fucosylated Lewis motifs that are associated with cell...

... that PKC ε 95 had a perinuclear, probably Golgi, localization and PKC ε 87 was distributed in the cytosol. It is proposed that phosphorylation at Ser-729 may be important for determining the intracellular localization of PKC ε, and that a specific Ser-729 phosphatase may be activated on cell passage...

... or more sites located within the secretory route proximal and distal to the site where ASA is retrieved by the KDEL receptor, i.e. proximal to the trans -Golgi. At each of these sites up to two N -acetylglucosamine 1-phosphate groups can be added to a single oligosaccharide. Of several drugs known...