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Keywords: Hill coefficient
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Evidence for co-operativity in coenzyme binding to tetrameric Sulfolobus solfataricus alcohol dehydrogenase and its structural basis: fluorescence, kinetic and structural studies of the wild-type enzyme and non-co-operative N249Y mutant

Antonietta GIORDANO, Ferdinando FEBBRAIO, Consiglia RUSSO, Mosè ROSSI, Carlo A. RAIA
Journal: Biochemical Journal
Biochem J (2005) 388 (2): 657–667.
https://doi.org/10.1042/BJ20041539
Published: 24 May 2005
...→Tyr) mutant was studied using the high fluorescence sensitivity of its tryptophan residues Trp-95 and Trp-117 to the binding of coenzyme moieties. Fluorescence quenching studies performed at 25 °C show that SsADH exhibits linearity in the NAD(H) binding [the Hill coefficient ( h )∼1) at pH 9.8...
View articletitled, Evidence for co-operativity in coenzyme binding to tetrameric Sulfolobus solfataricus alcohol dehydrogenase and its structural basis: fluorescence, kinetic and structural studies of the wild-type enzyme and non-co-operative N249Y mutant
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