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Structural Insights into allosteric inhibition of HRI kinase by heme binding via HDX-MS

Shivani Kanta, Vanesa Vinciauskaite, Graham Neill, Miratul Muqit, Glenn Masson

Biochem J (2025) BCJ20253072.

https://doi.org/10.1042/BCJ20253072

Published: 04 June 2025

...-threonine kinases Protein-tyrosine kinases Hydrogen-deuterium exchange mass spectrometry Stress response Iron The Royal Society http://dx.doi.org/10.13039/501100000188 RGS\R1\231147 Biotechnology and Biological Sciences Research Council http://dx.doi.org/10.13039/501100000268 BB...

View articletitled, Structural Insights into allosteric inhibition of HRI kinase by heme binding via HDX-MS

Articles

Metal-sensing properties of the disordered loop from the Arabidopsis metal transceptor IRT1

Virginia Cointry, Reyes Ródenas, Nelly Morellet, Steven Fanara, Valérie Cotelle, Julie Neveu, Grégory Vert

Biochem J (2025) 482 (09): 451–466.

https://doi.org/10.1042/BCJ20240685

Published: 06 May 2025

...Virginia Cointry; Reyes Ródenas; Nelly Morellet; Steven Fanara; Valérie Cotelle; Julie Neveu; Grégory Vert The plant iron-regulated transporter 1 (IRT1) iron transporter is a plasma membrane protein that takes up iron in the root under iron-limited conditions. Besides its primary metal substrate...

View articletitled, Metal-sensing properties of the disordered loop from the Arabidopsis metal transceptor IRT1

Includes: Supplementary data

Articles

Metal sensing properties of the disordered loop from the Arabidopsis metal transceptor IRT1

Virginia Cointry, Reyes Ródenas, Nelly Morellet, Steven Fanara, Valérie Cotelle, Julie Neveu, Grégory Vert

Biochem J (2025) BCJ20240685.

https://doi.org/10.1042/BCJ-2024-0685

Published: 17 April 2025

...Virginia Cointry; Reyes Ródenas; Nelly Morellet; Steven Fanara; Valérie Cotelle; Julie Neveu; Grégory Vert The plant IRT1 iron transporter is a plasma membrane protein that takes up iron in root upon iron-limited conditions. Besides its primary metal substrate iron, IRT1 transports other divalent...

View articletitled, Metal sensing properties of the disordered loop from the Arabidopsis metal transceptor IRT1

Articles

Insight into the function of active site residues in the catalytic mechanism of human ferrochelatase

Amy E. Medlock, Wided Najahi-Missaoui, Mesafint T. Shiferaw, Angela N. Albetel, William N. Lanzilotta, Harry A. Dailey,, Jr

Biochem J (2021) 478 (17): 3239–3252.

https://doi.org/10.1042/BCJ20210460

Published: 07 September 2021

...Amy E. Medlock; Wided Najahi-Missaoui; Mesafint T. Shiferaw; Angela N. Albetel; William N. Lanzilotta; Harry A. Dailey,, Jr Ferrochelatase catalyzes the insertion of ferrous iron into a porphyrin macrocycle to produce the essential cofactor, heme. In humans this enzyme not only catalyzes...

View articletitled, Insight into the function of active site residues in the catalytic mechanism of human ferrochelatase

Articles

Response to the letter ‘Absent regulation of iron acquisition by the copper regulator Mac1 in A. fumigatus ’

Yong-Sung Park, Suzie Kang, Hyewon Seo, Cheol-Won Yun

Biochem J (2020) 477 (16): 3055–3058.

https://doi.org/10.1042/BCJ20200341

Published: 26 August 2020

...Yong-Sung Park; Suzie Kang; Hyewon Seo; Cheol-Won Yun The answer to the letter ‘Absent regulation of iron acquisition by the copper regulator Mac1 in A. fumigatus ’ has been prepared. We explained our data and showed supplementary information to answer the questions. And we respect the results...

View articletitled, Response to the letter ‘Absent regulation of iron acquisition by the copper regulator Mac1 in A. fumigatus ’

Includes: Supplementary data

Articles

Absent regulation of iron acquisition by the copper regulator Mac1 in A. fumigatus

Annie Yap, Matthias Misslinger, Hubertus Haas

Biochem J (2020) 477 (16): 2967–2970.

https://doi.org/10.1042/BCJ20200286

Published: 19 August 2020

...Annie Yap; Matthias Misslinger; Hubertus Haas Aspergillus fumigatus is the most common cause of invasive aspergillosis, a life-threatening infection mainly affecting immunocompromised patients. The essential metals copper and iron play crucial roles in virulence of this mold. Recently, the copper...

View articletitled, Absent regulation of iron acquisition by the copper regulator Mac1 in A. fumigatus

Articles

Interaction of α-synuclein and Parkin in iron toxicity on SH-SY5Y cells: implications in the pathogenesis of Parkinson's disease

Upasana Ganguly, Anindita Banerjee, Sankha Shubhra Chakrabarti, Upinder Kaur, Oishimaya Sen, Roberto Cappai, Sasanka Chakrabarti

Biochem J (2020) 477 (6): 1109–1122.

https://doi.org/10.1042/BCJ20190676

Published: 23 March 2020

...Upasana Ganguly; Anindita Banerjee; Sankha Shubhra Chakrabarti; Upinder Kaur; Oishimaya Sen; Roberto Cappai; Sasanka Chakrabarti The toxicity of accumulated α-synuclein plays a key role in the neurodegeneration of Parkinson's disease (PD). This study has demonstrated that iron in varying...

View articletitled, Interaction of α-synuclein and Parkin in iron toxicity on SH-SY5Y cells: implications in the pathogenesis of Parkinson's disease

Includes: Supplementary data

Articles

Crystal structure of a hemerythrin-like protein from Mycobacterium kansasii and homology model of the orthologous Rv2633c protein of M. tuberculosis

Zhongxin Ma, Jan Abendroth, Garry W. Buchko, Kyle H. Rohde, Victor L. Davidson

Biochem J (2020) 477 (2): 567–581.

https://doi.org/10.1042/BCJ20190827

Published: 31 January 2020

...Zhongxin Ma; Jan Abendroth; Garry W. Buchko; Kyle H. Rohde; Victor L. Davidson Pathogenic and opportunistic mycobacteria have a distinct class of non-heme di-iron hemerythrin-like proteins (HLPs). The first to be isolated was the Rv2633c protein, which plays a role in infection by Mycobacterium...

View articletitled, Crystal structure of a hemerythrin-like protein from Mycobacterium kansasii and homology model of the orthologous Rv2633c protein of M. tuberculosis

Articles

Handing off iron to the next generation: how does it get into seeds and what for?

In Collection

Seeds

Stéphane Mari, Christophe Bailly, Sébastien Thomine

Biochem J (2020) 477 (1): 259–274.

https://doi.org/10.1042/BCJ20190188

Published: 17 January 2020

...Stéphane Mari; Christophe Bailly; Sébastien Thomine To ensure the success of the new generation in annual species, the mother plant transfers a large proportion of the nutrients it has accumulated during its vegetative life to the next generation through its seeds. Iron (Fe) is required in large...

View articletitled, Handing off iron to the next generation: how does it get into seeds and what for?

Articles

Group A Streptococcus co-ordinates manganese import and iron efflux in response to hydrogen peroxide stress

Andrew G. Turner, Karrera Y. Djoko, Cheryl-lynn Y. Ong, Timothy C. Barnett, Mark J. Walker, Alastair G. McEwan

Biochem J (2019) 476 (3): 595–611.

https://doi.org/10.1042/BCJ20180902

Published: 14 February 2019

... – 26 ]. The Fe-loaded form of PerR is highly sensitive to oxidation by H 2 O 2 , which in turn enables derepression of genes involved in the oxidative stress response [ 27 ]. In GAS, these genes include pmtA , which encodes a P 1B-4 -type ATPase that effluxes ferrous iron and likely reduces...

View articletitled, Group A Streptococcus co-ordinates manganese import and iron efflux in response to hydrogen peroxide stress

Includes: Supplementary data

Articles

A copper transcription factor, AfMac1, regulates both iron and copper homeostasis in the opportunistic fungal pathogen Aspergillus fumigatus

Yong-Sung Park, Suzie Kang, Hyewon Seo, Cheol-Won Yun

Biochem J (2018) 475 (17): 2831–2845.

https://doi.org/10.1042/BCJ20180399

Published: 11 September 2018

...Yong-Sung Park; Suzie Kang; Hyewon Seo; Cheol-Won Yun Although iron and copper are co-ordinately regulated in living cells, the homeostatic effects of each of these metals on the other remain unknown. Here, we show the function of AfMac1, a transcriptional activator of the copper and iron regulons...

View articletitled, A copper transcription factor, AfMac1, regulates both iron and copper homeostasis in the opportunistic fungal pathogen Aspergillus fumigatus

Includes: Supplementary data

Articles

Functional characterization of the copper transcription factor AfMac1 from Aspergillus fumigatus

Yong-Sung Park, Tae-Hyoung Kim, Cheol-Won Yun

Biochem J (2017) 474 (14): 2365–2378.

https://doi.org/10.1042/BCJ20170191

Published: 03 July 2017

... in A. fumigatus . Correspondence: Cheol-Won Yun ( [email protected] ) 10 3 2017 14 5 2017 16 5 2017 17 5 2017 © 2017 The Author(s); published by Portland Press Limited on behalf of the Biochemical Society 2017 A. fumigatus copper iron Mac1 MrpA S. cerevisiae...

View articletitled, Functional characterization of the copper transcription factor AfMac1 from Aspergillus fumigatus

Articles

Identification of ferrichrome- and ferrioxamine B-mediated iron uptake by Aspergillus fumigatus

Yong-Sung Park, Ju-Yeon Kim, Cheol-Won Yun

Biochem J (2016) 473 (9): 1203–1213.

https://doi.org/10.1042/BCJ20160066

Published: 26 April 2016

... are putative virulence factors of A. fumigatus ; sit1 and sit2 are homologous to yeast Sit1, and sit1 and sit2 gene expression was up-regulated after iron depletion. When expressed heterologously in Saccharomyces cerevisiae , sit1 and sit2 were localized to the plasma membrane ; sit1 efficiently complemented...

View articletitled, Identification of ferrichrome- and ferrioxamine B-mediated iron uptake by Aspergillus fumigatus

Includes: Supplementary data

Articles

An essential role for functional lysosomes in ferroptosis of cancer cells

Seiji Torii, Ryosuke Shintoku, Chisato Kubota, Makoto Yaegashi, Ryoko Torii, Masaya Sasaki, Toshinobu Suzuki, Masanobu Mori, Yuhei Yoshimoto, Toshiyuki Takeuchi, Keiichi Yamada

Biochem J (2016) 473 (6): 769–777.

https://doi.org/10.1042/BJ20150658

Published: 10 March 2016

..., which requires intracellular iron. In the present study, we assessed ferroptosis following treatment of human fibrosarcoma HT1080 cells with several inhibitors of lysosomal activity and found that they prevented cell death induced by the ferroptosis-inducing compounds erastin and RSL3. Fluorescent...

View articletitled, An essential role for functional lysosomes in ferroptosis of cancer cells

Includes: Supplementary data

Articles

Iron alters cell survival in a mitochondria-dependent pathway in ovarian cancer cells

Kyle Bauckman, Edward Haller, Nicholas Taran, Stephanie Rockfield, Abigail Ruiz-Rivera, Meera Nanjundan

Biochem J (2015) 466 (2): 401–413.

https://doi.org/10.1042/BJ20140878

Published: 20 February 2015

...Kyle Bauckman; Edward Haller; Nicholas Taran; Stephanie Rockfield; Abigail Ruiz-Rivera; Meera Nanjundan The role of iron in the development of cancer remains unclear. We previously reported that iron reduces cell survival in a Ras/mitogen-activated protein kinase (MAPK)-dependent manner in ovarian...

View articletitled, Iron alters cell survival in a mitochondria-dependent pathway in ovarian cancer cells

Includes: Supplementary data

Articles

Loss of TRPML1 promotes production of reactive oxygen species: is oxidative damage a factor in mucolipidosis type IV?

Jessica Coblentz, Claudette St. Croix, Kirill Kiselyov

Biochem J (2014) 457 (2): 361–368.

https://doi.org/10.1042/BJ20130647

Published: 20 December 2013

... that TRPML1's role in the cell extends outside lysosomes. 1 To whom correspondence should be addressed (email [email protected] ). 9 5 2013 31 10 2013 6 11 2013 6 11 2013 iron lysosomal storage disease mitochondrion reactive oxygen species transient receptor potential...

View articletitled, Loss of TRPML1 promotes production of reactive oxygen species: is oxidative damage a factor in mucolipidosis type IV?

Includes: Supplementary data

Articles

Structural and functional characterization of the Staphylococcus aureus virulence factor and vaccine candidate FhuD2

Paolo Mariotti, Enrico Malito, Marco Biancucci, Paola Lo Surdo, Ravi P. N. Mishra, Vincenzo Nardi-Dei, Silvana Savino, Mikkel Nissum, Glen Spraggon, Guido Grandi, Fabio Bagnoli, Matthew J. Bottomley

Biochem J (2013) 449 (3): 683–693.

https://doi.org/10.1042/BJ20121426

Published: 09 January 2013

.... The development of antibiotic resistance in S. aureus highlights the need for a preventive vaccine. In the present paper we explore the structure and function of FhuD2 (ferric-hydroxamate uptake D2), a staphylococcal surface lipoprotein mediating iron uptake during invasive infection, recently described...

View articletitled, Structural and functional characterization of the Staphylococcus aureus virulence factor and vaccine candidate FhuD2

Includes: Supplementary data

Articles

TfR1 interacts with the IKK complex and is involved in IKK–NF-κB signalling

Niall S. Kenneth, Sharon Mudie, Sanne Naron, Sonia Rocha

Biochem J (2013) 449 (1): 275–284.

https://doi.org/10.1042/BJ20120625

Published: 07 December 2012

... an unbiased proteomics screen where we identified TfR1 (transferrin receptor 1). TfR1 is required for transferrin binding and internalization and ultimately for iron homoeostasis. TfR1 depletion does not lead to changes in IKK subunit protein levels; however, it does reduce the formation of the IKK complex...

View articletitled, TfR1 interacts with the IKK complex and is involved in IKK–NF-κB signalling

Includes: Supplementary data

Articles

Mutation in the Fe–S scaffold protein Isu bypasses frataxin deletion

Heeyong Yoon, Ramesh Golla, Emmanuel Lesuisse, Jayashree Pain, Jason E. Donald, Elise R. Lyver, Debkumar Pain, Andrew Dancis

Biochem J (2012) 441 (1): 473–480.

https://doi.org/10.1042/BJ20111637

Published: 14 December 2011

...Heeyong Yoon; Ramesh Golla; Emmanuel Lesuisse; Jayashree Pain; Jason E. Donald; Elise R. Lyver; Debkumar Pain; Andrew Dancis Frataxin is a conserved mitochondrial protein deficient in patients with Friedreich's ataxia. Frataxin has been implicated in control of iron homoeostasis and Fe–S cluster...

View articletitled, Mutation in the Fe–S scaffold protein Isu bypasses frataxin deletion

Articles

Rim2, a pyrimidine nucleotide exchanger, is needed for iron utilization in mitochondria

Heeyong Yoon, Yan Zhang, Jayashree Pain, Elise R. Lyver, Emmanuel Lesuisse, Debkumar Pain, Andrew Dancis

Biochem J (2011) 440 (1): 137–146.

https://doi.org/10.1042/BJ20111036

Published: 27 October 2011

...Heeyong Yoon; Yan Zhang; Jayashree Pain; Elise R. Lyver; Emmanuel Lesuisse; Debkumar Pain; Andrew Dancis Mitochondria transport and utilize iron for the synthesis of haem and Fe–S clusters. Although many proteins are known to be involved in these processes, additional proteins are likely...

View articletitled, Rim2, a pyrimidine nucleotide exchanger, is needed for iron utilization in mitochondria

Articles

Entamoeba histolytica secretes two haem-binding proteins to scavenge haem

Areli Cruz-Castañeda, Mavil López-Casamichana, José J. Olivares-Trejo

Biochem J (2011) 434 (1): 105–111.

https://doi.org/10.1042/BJ20100897

Published: 27 January 2011

...Areli Cruz-Castañeda; Mavil López-Casamichana; José J. Olivares-Trejo Entamoeba histolytica is a human pathogen which can grow using different sources of iron such as free iron, lactoferrin, transferrin, ferritin or haemoglobin. In the present study, we found that E. histolytica was also capable...

View articletitled, Entamoeba histolytica secretes two haem-binding proteins to scavenge haem

Articles

The role of vicinal tyrosine residues in the function of Haemophilus influenzae ferric-binding protein A

Husain K. Khambati, Trevor F. Moraes, Jagroop Singh, Stephen R. Shouldice, Rong-hua Yu, Anthony B. Schryvers

Biochem J (2010) 432 (1): 57–67.

https://doi.org/10.1042/BJ20101043

Published: 25 October 2010

...Husain K. Khambati; Trevor F. Moraes; Jagroop Singh; Stephen R. Shouldice; Rong-hua Yu; Anthony B. Schryvers The periplasmic FbpA (ferric-binding protein A) from Haemophilus influenzae plays a critical role in acquiring iron from host transferrin, shuttling iron from the outer-membrane receptor...

View articletitled, The role of vicinal tyrosine residues in the function of Haemophilus influenzae ferric-binding protein A

Includes: Supplementary data

Articles

Antagonistic roles of the ERK and p38 MAPK signalling pathways in globin expression, haem biosynthesis and iron uptake 1

Louay Mardini, Jadwiga Gasiorek, Anna Derjuga, Lucie Carrière, Matthias Schranzhofer, Barry H. Paw, Prem Ponka, Volker Blank

Biochem J (2010) 432 (1): 145–151.

https://doi.org/10.1042/BJ20100541

Published: 25 October 2010

...Louay Mardini; Jadwiga Gasiorek; Anna Derjuga; Lucie Carrière; Matthias Schranzhofer; Barry H. Paw; Prem Ponka; Volker Blank Late-stage erythroid cells synthesize large quantities of haemoglobin, a process requiring the co-ordinated regulation of globin and haem synthesis as well as iron uptake...

View articletitled, Antagonistic roles of the ERK and p38 MAPK signalling pathways in globin expression, haem biosynthesis and iron uptake 1

Articles

Cadmium regulates copper homoeostasis by inhibiting the activity of Mac1, a transcriptional activator of the copper regulon, in Saccharomyces cerevisiae

Dong-Hyuk Heo, In-Joon Baek, Hyun-Jun Kang, Ji-Hyun Kim, Miwha Chang, Mi-Young Jeong, Tae-Hyoung Kim, Il-Dong Choi, Cheol-Won Yun

Biochem J (2010) 431 (2): 257–265.

https://doi.org/10.1042/BJ20100638

Published: 28 September 2010

... of Fet3 activity, which eventually impairs iron uptake. In this way, cadmium exhibits a negative effect on both iron and copper homoeostasis. 1 To whom correspondence should be addressed ( [email protected] ). 23 4 2010 29 7 2010 30 7 2010 30 7 2010 © The Authors...

View articletitled, Cadmium regulates copper homoeostasis by inhibiting the activity of Mac1, a transcriptional activator of the copper regulon, in Saccharomyces cerevisiae

Articles

Transferrin-iron routing to the cytosol and mitochondria as studied by live and real-time fluorescence

Maya Shvartsman, Eitan Fibach, Z. Ioav Cabantchik

Biochem J (2010) 429 (1): 185–193.

https://doi.org/10.1042/BJ20100213

Published: 14 June 2010

...Maya Shvartsman; Eitan Fibach; Z. Ioav Cabantchik In the present study we analysed the mechanism of intracellular routing of iron acquired by erythroid cells via receptor-mediated endocytosis of Tf-Fe [Tf (transferrin)-iron]. Using real-time fluorimetry and flow cytometry, in conjunction...

View articletitled, Transferrin-iron routing to the cytosol and mitochondria as studied by live and real-time fluorescence

Articles

Characterization of the transition-metal-binding properties of hepcidin

Chris Tselepis, Samuel J. Ford, Andrew T. McKie, Wolfgang Vogel, Heinz Zoller, Robert J. Simpson, Javier Diaz Castro, Tariq H. Iqbal, Douglas G. Ward

Biochem J (2010) 427 (2): 289–296.

https://doi.org/10.1042/BJ20091521

Published: 29 March 2010

...Chris Tselepis; Samuel J. Ford; Andrew T. McKie; Wolfgang Vogel; Heinz Zoller; Robert J. Simpson; Javier Diaz Castro; Tariq H. Iqbal; Douglas G. Ward Accumulating evidence suggests that hepcidin, a 25-residue peptide hormone, is the master regulator of iron metabolism. Further evidence suggests...

View articletitled, Characterization of the transition-metal-binding properties of hepcidin

Articles

Gain-of-function mutations identify amino acids within transmembrane domains of the yeast vacuolar transporter Zrc1 that determine metal specificity

Huilan Lin, Damali Burton, Liangtao Li, David E. Warner, John D. Phillips, Diane McVey Ward, Jerry KAPLAN

Biochem J (2009) 422 (2): 273–283.

https://doi.org/10.1042/BJ20090853

Published: 13 August 2009

... and Kaplan (2008) J. Biol. Chem. 283 , 33865–33873]. Mutant Zrc1 that gained iron transport activity could protect cells with a deletion in the vacuolar iron transporter ( CCC1 ) from high iron toxicity. Utilizing suppression of high iron toxicity and PCR mutagenesis of ZRC1 , we identified other amino acid...

View articletitled, Gain-of-function mutations identify amino acids within transmembrane domains of the yeast vacuolar transporter Zrc1 that determine metal specificity

Articles

A novel function of Aft1 in regulating ferrioxamine B uptake: Aft1 modulates Arn3 ubiquitination in Saccharomyces cerevisiae

Mi-Young Jeong, Chang-Min Kang, Ji-Hyun Kim, Dong-Hyuk Heo, Miwha Chang, In-Joon Baek, Hyeon-Su Ro, Il-Dong Choi, Tae-Hyoung Kim, Cheol-Won Yun

Biochem J (2009) 422 (1): 181–191.

https://doi.org/10.1042/BJ20082399

Published: 29 July 2009

...Mi-Young Jeong; Chang-Min Kang; Ji-Hyun Kim; Dong-Hyuk Heo; Miwha Chang; In-Joon Baek; Hyeon-Su Ro; Il-Dong Choi; Tae-Hyoung Kim; Cheol-Won Yun Aft1 is a transcriptional activator in Saccharomyces cerevisiae that responds to iron availability and regulates the expression of genes in the iron...

View articletitled, A novel function of Aft1 in regulating ferrioxamine B uptake: Aft1 modulates Arn3 ubiquitination in Saccharomyces cerevisiae

Articles

Haemolytic anaemia and alterations in hepatic iron metabolism in aged mice lacking Cu,Zn-superoxide dismutase

Rafał R. Starzyński, François Canonne-Hergaux, Alexandra Willemetz, Mikołaj A. Gralak, Jarosław Woliński, Agnieszka Styś, Jarosław Olszak, Paweł Lipiński

Biochem J (2009) 420 (3): 383–390.

https://doi.org/10.1042/BJ20082137

Published: 27 May 2009

...Rafał R. Starzyński; François Canonne-Hergaux; Alexandra Willemetz; Mikołaj A. Gralak; Jarosław Woliński; Agnieszka Styś; Jarosław Olszak; Paweł Lipiński The continuous recycling of haem iron following phagocytosis and catabolism of senescent and damaged red blood cells by macrophages is a crucial...

View articletitled, Haemolytic anaemia and alterations in hepatic iron metabolism in aged mice lacking Cu,Zn-superoxide dismutase

Includes: Supplementary data

Articles

Arabidopsis thaliana GLX2-1 contains a dinuclear metal binding site, but is not a glyoxalase 2

Pattraranee Limphong, Michael W. Crowder, Brian Bennett, Christopher A. Makaroff

Biochem J (2009) 417 (1): 323–330.

https://doi.org/10.1042/BJ20081151

Published: 12 December 2008

..., kinetics, and UV–visible, EPR, and 1 H-NMR spectroscopies. The purified enzyme was purple and contained substoichiometric amounts of iron and zinc; however, metal-binding studies reveal that GLX2-1 can bind nearly two equivalents of either iron or zinc and that the most stable analogue of GLX2-1...

View articletitled, Arabidopsis thaliana GLX2-1 contains a dinuclear metal binding site, but is not a glyoxalase 2

Articles

Tyrosine modification enhances metal-ion binding

Graham S. Baldwin, Michael F. Bailey, B. Philip Shehan, Ioulia Sims, Raymond S. Norton

Biochem J (2008) 416 (1): 77–84.

https://doi.org/10.1042/BJ20081059

Published: 28 October 2008

...). calcium cholecystokinin ferric ion iron phosphotyrosine sulfotyrosine Modification of tyrosine residues is a critical post-translational modification of many proteins. Tyrosine sulfation occurs in the trans -Golgi network, and is thought to be important in protein secretion [ 1 , 2 ]. A more...

View articletitled, Tyrosine modification enhances metal-ion binding

Includes: Supplementary data

Articles

The crystal structure of peroxymyoglobin generated through cryoradiolytic reduction of myoglobin compound III during data collection

Hans-Petter Hersleth, Ya-Wen Hsiao, Ulf Ryde, Carl Henrik Görbitz, K. Kristoffer Andersson

Biochem J (2008) 412 (2): 257–264.

https://doi.org/10.1042/BJ20070921

Published: 14 May 2008

... be addressed (email [email protected] ). 12 7 2007 22 1 2008 23 1 2008 23 1 2008 © The Authors Journal compilation © 2008 Biochemical Society 2008 cryoradiolytic haem iron myoglobin oxygen binding peroxide complex Mb (myoglobin) is a haem protein...

View articletitled, The crystal structure of peroxymyoglobin generated through cryoradiolytic reduction of myoglobin compound III during data collection

Articles

Sequential regulation of ferroportin expression after erythrophagocytosis in murine macrophages: early mRNA induction by haem, followed by iron-dependent protein expression

Constance Delaby, Nathalie Pilard, Hervé Puy, François Canonne-Hergaux

Biochem J (2008) 411 (1): 123–131.

https://doi.org/10.1042/BJ20071474

Published: 13 March 2008

...Constance Delaby; Nathalie Pilard; Hervé Puy; François Canonne-Hergaux Tissue macrophages play an essential role in iron recycling through the phagocytosis of senescent RBCs (red blood cells). Following haem catabolism by HO1 (haem oxygenase 1), they recycle iron back into the plasma through...

View articletitled, Sequential regulation of ferroportin expression after erythrophagocytosis in murine macrophages: early mRNA induction by haem, followed by iron-dependent protein expression

Includes: Supplementary data

Articles

Physical and functional interaction of FgFtr1–FgFet1 and FgFtr2–FgFet2 is required for iron uptake in Fusarium graminearum

Yong-Sung Park, Ji-Hyun Kim, Jin-Hwa Cho, Hyo-Ihl Chang, Seung-Wook Kim, Hyun-Dong Paik, Chang-Won Kang, Tae-Hyoung Kim, Ha-Chin Sung, Cheol-Won Yun

Biochem J (2007) 408 (1): 97–104.

https://doi.org/10.1042/BJ20070450

Published: 29 October 2007

...Yong-Sung Park; Ji-Hyun Kim; Jin-Hwa Cho; Hyo-Ihl Chang; Seung-Wook Kim; Hyun-Dong Paik; Chang-Won Kang; Tae-Hyoung Kim; Ha-Chin Sung; Cheol-Won Yun FgFtr1 and FgFtr2 are putative iron permeases, and FgFet1 and FgFet2 are putative ferroxidases of Fusarium graminearum . They have high homologies...

View articletitled, Physical and functional interaction of FgFtr1–FgFet1 and FgFtr2–FgFet2 is required for iron uptake in Fusarium graminearum

Includes: Supplementary data

Articles

Activation of brain calcineurin (Cn) by Cu-Zn superoxide dismutase (SOD1) depends on direct SOD1–Cn protein interactions occurring in vitro and in vivo

Abdulbaki Agbas, Dongwei Hui, Xinsheng Wang, Vekalet Tek, Asma Zaidi, Elias K. Michaelis

Biochem J (2007) 405 (1): 51–59.

https://doi.org/10.1042/BJ20061202

Published: 13 June 2007

... The Authors Journal compilation © 2007 Biochemical Society 2007 brain calcineurin hydrogen peroxide iron superoxide dismutase zinc Cn (calcineurin) is an Fe- and Zn-containing metallophosphatase [ 1 – 6 ]. The active form of the enzyme is a heterodimer of CnA and CnB. Activation of Cn...

View articletitled, Activation of brain calcineurin (Cn) by Cu-Zn superoxide dismutase (SOD1) depends on direct SOD1–Cn protein interactions occurring in vitro and in vivo

Articles

Does the calcein-AM method assay the total cellular ‘labile iron pool’ or only a fraction of it?

Margarita Tenopoulou, Tino Kurz, Paschalis-Thomas Doulias, Dimitrios Galaris, Ulf T. Brunk

Biochem J (2007) 403 (2): 261–266.

https://doi.org/10.1042/BJ20061840

Published: 26 March 2007

...Margarita Tenopoulou; Tino Kurz; Paschalis-Thomas Doulias; Dimitrios Galaris; Ulf T. Brunk The calcein-AM (calcein-acetoxymethyl ester) method is a widely used technique that is supposed to assay the intracellular ‘labile iron pool’ (LIP). When cells in culture are exposed to this ester, it passes...

View articletitled, Does the calcein-AM method assay the total cellular ‘labile iron pool’ or only a fraction of it?

Articles

Comparison of mammalian cell lines expressing distinct isoforms of divalent metal transporter 1 in a tetracycline-regulated fashion

Michael D. Garrick, Hung-Chieh Kuo, Farida Vargas, Steven Singleton, Lin Zhao, Jaime J. Smith, Prasad Paradkar, Jerome A. Roth, Laura M. Garrick

Biochem J (2006) 398 (3): 539–546.

https://doi.org/10.1042/BJ20051987

Published: 29 August 2006

...Michael D. Garrick; Hung-Chieh Kuo; Farida Vargas; Steven Singleton; Lin Zhao; Jaime J. Smith; Prasad Paradkar; Jerome A. Roth; Laura M. Garrick DMT1 (divalent metal transporter; also known as SLC11A2, DCT1 or Nramp2) is responsible for ferrous iron uptake in the duodenum, iron exit from endosomes...

View articletitled, Comparison of mammalian cell lines expressing distinct isoforms of divalent metal transporter 1 in a tetracycline-regulated fashion

Includes: Supplementary data

Articles

Tumour necrosis factor α causes hypoferraemia and reduced intestinal iron absorption in mice

Abas H. Laftah, Naveen Sharma, Matthew J. Brookes, Andrew T. McKie, Robert J. Simpson, Tariq H. Iqbal, Chris Tselepis

Biochem J (2006) 397 (1): 61–67.

https://doi.org/10.1042/BJ20060215

Published: 14 June 2006

...Abas H. Laftah; Naveen Sharma; Matthew J. Brookes; Andrew T. McKie; Robert J. Simpson; Tariq H. Iqbal; Chris Tselepis Cytokines are implicated in the anaemia of chronic disease by reducing erythropoiesis and increasing iron sequestration in the reticuloendotheial system. However, the effect...

View articletitled, Tumour necrosis factor α causes hypoferraemia and reduced intestinal iron absorption in mice

Articles

Transferrin receptor 2 protein is not expressed in normal erythroid cells

Alessia CALZOLARI, Silvia DEAGLIO, Nadia Maria SPOSI, Eleonora PETRUCCI, Ornella MORSILLI, Marco GABBIANELLI, Fabio MALAVASI, Cesare PESCHLE, Ugo TESTA

Biochem J (2004) 381 (3): 629–634.

https://doi.org/10.1042/BJ20040230

Published: 27 July 2004

.... 1 To whom correspondence should be addressed (e-mail [email protected] ). 11 2 2004 31 3 2004 15 4 2004 15 4 2004 The Biochemical Society, London 2004 erythropoiesis haemochromatosis iron liver transferrin transferrin receptor Erythroleukaemic K562...

View articletitled, Transferrin receptor 2 protein is not expressed in normal erythroid cells

Articles

Complex changes in cellular inositol phosphate complement accompany transit through the cell cycle

Christopher J. BARKER, Joanne WRIGHT, Philip J. HUGHES, Christopher J. KIRK, Robert H. MICHELL

Biochem J (2004) 380 (2): 465–473.

https://doi.org/10.1042/bj20031872

Published: 01 June 2004

... with cell-cycle progression. Key words: cell cycle, inositol polyphosphate, inositol 1,2,3- trisphosphate, iron, WRK-1 cells. INTRODUCTION Phosphoinositide-based regulatory systems are ubiquitous in eukaryotes. They contribute to many processes, including signal- ling from cell-surface receptors, vesicle...

View articletitled, Complex changes in cellular inositol phosphate complement accompany transit through the cell cycle

Articles

Metallothionein is crucial for safe intracellular copper storage and cell survival at normal and supra-physiological exposure levels

Lucía TAPIA, Mauricio GONZÁLEZ-AGÜERO, Mónica F. CISTERNAS, Miriam SUAZO, Verónica CAMBIAZO, Ricardo UAUY, Mauricio GONZÁLEZ

Biochem J (2004) 378 (2): 617–624.

https://doi.org/10.1042/bj20031174

Published: 01 March 2004

... words: copper, efflux, iron, metallothionein, uptake, zinc. INTRODUCTION Cu (copper) is an essential micronutrient for life. It is required by a wide range of species, from bacteria to yeast, plants and mammals, including humans [1]. The essential requirement for Cu is based on its capacity to act...

View articletitled, Metallothionein is crucial for safe intracellular copper storage and cell survival at normal and supra-physiological exposure levels

Articles

Role of YHM1, encoding a mitochondrial carrier protein, in iron distribution of yeast

Emmanuel LESUISSE, Elise R. LYVER, Simon A. B. KNIGHT, Andrew DANCIS

Biochem J (2004) 378 (2): 599–607.

https://doi.org/10.1042/bj20031387

Published: 01 March 2004

... or mitochondria to cytoplasm, although many family members do not have assigned substrates. We speculated whether one or more of these transporters will play a role in iron metabolism. Haploid yeast strains each deleted for a single mitochondrial carrier protein were analysed for alterations in iron homoeostasis...

View articletitled, Role of YHM1, encoding a mitochondrial carrier protein, in iron distribution of yeast

Articles

Functional studies of hephaestin in yeast: evidence for multicopper oxidase activity in the endocytic pathway

Liangtao LI, Chris D. VULPE, Jerry KAPLAN

Biochem J (2003) 375 (3): 793–798.

https://doi.org/10.1042/bj20030866

Published: 01 November 2003

...Liangtao LI; Chris D. VULPE; Jerry KAPLAN Hephaestin is a mammalian gene that encodes a predicted multicopper oxidase required for intestinal iron export. To examine if hephaestin can act as a ferroxidase, we studied yeast strains transformed with plasmids containing both a full-length hephaestin...

View articletitled, Functional studies of hephaestin in yeast: evidence for multicopper oxidase activity in the endocytic pathway

Articles

Zinc suppresses the iron-accumulation phenotype of Saccharomyces cerevisiae lacking the yeast frataxin homologue (Yfh1)

Renata SANTOS, Andrew DANCIS, David EIDE, Jean-Michel CAMADRO, Emmanuel LESUISSE

Biochem J (2003) 375 (2): 247–254.

https://doi.org/10.1042/bj20030835

Published: 15 October 2003

...Renata SANTOS; Andrew DANCIS; David EIDE; Jean-Michel CAMADRO; Emmanuel LESUISSE Analysis of Saccharomyces cerevisiae cell transcriptome revealed that iron deprivation/supplementation affects genes other than those of the iron regulon (controlled by Aft proteins). Several genes regulated by zinc...

View articletitled, Zinc suppresses the iron-accumulation phenotype of Saccharomyces cerevisiae lacking the yeast frataxin homologue (Yfh1)

Articles

Effects of nitrogen monoxide and carbon monoxide on molecular and cellular iron metabolism: mirror-image effector molecules that target iron

Ralph N. WATTS, Prem PONKA, Des R. RICHARDSON

Biochem J (2003) 369 (3): 429–440.

https://doi.org/10.1042/bj20021302

Published: 01 February 2003

...Ralph N. WATTS; Prem PONKA; Des R. RICHARDSON Many effector functions of nitrogen monoxide (NO) and carbon monoxide (CO) are mediated through their high-affinity for iron (Fe). In this review, the roles of NO and CO are examined in terms of their effects on the molecular and cellular mechanisms...

View articletitled, Effects of nitrogen monoxide and carbon monoxide on molecular and cellular iron metabolism: mirror-image effector molecules that target iron

Articles

Inhibition of potato lipoxygenase by linoleyl hydroxamic acid: kinetic and EPR spectral evidence for a two-step reaction

Igor A. BUTOVICH, C. Channa REDDY

Biochem J (2002) 365 (3): 865–871.

https://doi.org/10.1042/bj20020495

Published: 01 August 2002

... iron was in ferrous state. The positional isomer 9( S )-HPODE caused similar spectral changes. Therefore the catalytic centre of ptLOX appears to accommodate both positional isomers of linoleic acid hydroperoxides in a manner that ensures proper alignment of their hydroperoxy groups with the iron...

View articletitled, Inhibition of potato lipoxygenase by linoleyl hydroxamic acid: kinetic and EPR spectral evidence for a two-step reaction

Articles

Expression and purification of functional recombinant meningococcal transferrin-binding protein A

Jonathan S. OAKHILL, Christopher L. JOANNOU, Susan K. BUCHANAN, Andrew R. GORRINGE, Robert W. EVANS

Biochem J (2002) 364 (3): 613–616.

https://doi.org/10.1042/bj20020500

Published: 15 June 2002

...Jonathan S. OAKHILL; Christopher L. JOANNOU; Susan K. BUCHANAN; Andrew R. GORRINGE; Robert W. EVANS Pathogenic bacteria of the genus Neisseria have a siderophore-independent iron-uptake system reliant on a direct interaction between the bacterial cell and human transferrin (hTf), a serum protein...

View articletitled, Expression and purification of functional recombinant meningococcal transferrin-binding protein A

Articles

Structure and differential expression of the four members of the Arabidopsis thaliana ferritin gene family

Jean-Michel PETIT, Jean-François BRIAT, Stéphane LOBRÉAUX

Biochem J (2001) 359 (3): 575–582.

https://doi.org/10.1042/bj3590575

Published: 25 October 2001

... peptide in the mature subunit. Specific residues for ferroxidase activity and iron nucleation, which are found respectively in H-type or L-type ferritin subunits in animals, are both conserved within each of the four A. thaliana ferritin polypeptides. In addition, the hydrophilic nature of the plant...

View articletitled, Structure and differential expression of the four members of the Arabidopsis thaliana ferritin gene family

Articles

Ferritin and the response to oxidative stress

Kouichi ORINO, Lori LEHMAN, Yoshiaki TSUJI, Hitoshi AYAKI, Suzy V. TORTI, Frank M. TORTI

Biochem J (2001) 357 (1): 241–247.

https://doi.org/10.1042/bj3570241

Published: 25 June 2001

...Kouichi ORINO; Lori LEHMAN; Yoshiaki TSUJI; Hitoshi AYAKI; Suzy V. TORTI; Frank M. TORTI Iron is required for normal cell growth and proliferation. However, excess iron is potentially harmful, as it can catalyse the formation of toxic reactive oxygen species (ROS) via Fenton chemistry...

View articletitled, Ferritin and the response to oxidative stress

Articles

Apoptosis induced by exposure to a low steady-state concentration of H2O2 is a consequence of lysosomal rupture

Fernando ANTUNES, Enrique CADENAS, Ulf T. BRUNK

Biochem J (2001) 356 (2): 549–555.

https://doi.org/10.1042/bj3560549

Published: 24 May 2001

... to H 2 O 2 ; (iii) both events were inhibited by pre-treatment with iron chelators, including desferrioxamine. This compound is known to be taken up by endocytosis only and thus to become localized in the lysosomal compartment. After exposure to oxidative stress, when cells were again in standard...

View articletitled, Apoptosis induced by exposure to a low steady-state concentration of H2O2 is a consequence of lysosomal rupture

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