... spectrum of pharmacological properties. In the present study, we show that DIM is a potent inhibitor of Leishmania donovani topoisomerase I with an IC 50 of 1.2 μM. Equilibrium dialysis shows that DIM binds strongly to the free enzyme with a binding constant of 9.73×10 −9 M. The binding affinity of DIM...

...) Biochem. J. 387 , 591–600], the mechanisms that determine binding of substrate and cause product inhibition of adenosine kinase from Leishmania donovani remained unclear. In the present study, employing homology-model-guided site-specific protein mutagenesis, we show that Asp 16 is indispensable, since...

... (glyoxalase II) has been cloned from Leishmania donovani , a protozoan parasite that causes visceral leishmaniasis. DNA sequence analysis revealed an ORF (open reading frame) of ∼888 bp that encodes a putative 295-amino-acid protein with a calculated molecular mass of 32.5 kDa and a predicted pI of 6.0...

...Rupak DATTA; Ishita DAS; Banibrata SEN; Anutosh CHAKRABORTY; Subrata ADAK; Chhabinath MANDAL; Alok K. DATTA Leishmania donovani adenosine kinase (LdAdK) plays a pivotal role in scavenging of purines from the host. Exploiting interspecies homology and structural co-ordinates of the enzyme from other...

...Marie-Pierre HASNE; Françoise LAWRENCE Prenylated protein methyltransferase, an enzyme involved in the post-translational modification of many signalling proteins, has been characterized in a parasitic flagellated protozoan, Leishmania donovani. The activity of this enzyme was monitored...

... activity of antisense oligonucleotides. 1 To whom correspondence should be addressed (e-mail [email protected]). 10 9 1998 19 1 1999 10 3 1999 The Biochemical Society, London © 1999 1999 antisense oligonucleotides cationic liposomes Leishmania donovani...