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Keywords: Mycobacterium tuberculosis
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Articles

Insights into the central role of N-acetyl-glucosamine-1-phosphate uridyltransferase (GlmU) in peptidoglycan metabolism and its potential as a therapeutic target

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Vijay Soni, Eric H. Rosenn, Ramya Venkataraman
Journal: Biochemical Journal
Biochem J (2023) 480 (15): 1147–1164.
https://doi.org/10.1042/BCJ20230173
Published: 27 July 2023
... and their significance in aiding future drug discoveries. Both peptidoglycan and arabinogalactan are extensively altered with amide, succinyl, glycolyl, and galactosamine residues [ 50 , 114 , 115 ]. Some bacteria such as Mycobacterium tuberculosis display unusual 3–3 peptide crosslinking instead of traditional 4–3...
View articletitled, Insights into the central role of N-acetyl-glucosamine-1-phosphate uridyltransferase (GlmU) in peptidoglycan metabolism and its potential as a therapeutic target
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Polypharmacological repurposing approach identifies approved drugs as potential inhibitors of Mycobacterium tuberculosis

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Jasdeep Singh, Neha Quadir, Shubham Vashishtha, Ankan Chakraborty, Anwar Alam, Bishwajit Kundu, Uzair Ahmad, Durai Sundar, Nasreen Z. Ehtesham, Seyed E. Hasnain
Journal: Biochemical Journal
Biochem J (2023) 480 (14): 1079–1096.
https://doi.org/10.1042/BCJ20230143
Published: 17 July 2023
...Jasdeep Singh; Neha Quadir; Shubham Vashishtha; Ankan Chakraborty; Anwar Alam; Bishwajit Kundu; Uzair Ahmad; Durai Sundar; Nasreen Z. Ehtesham; Seyed E. Hasnain Mycobacterium tuberculosis ( M. tb ), the causative pathogen of tuberculosis (TB) remains the leading cause of death from single...
View articletitled, Polypharmacological repurposing approach identifies approved drugs as potential inhibitors of Mycobacterium tuberculosis
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Includes: Supplementary data
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Functional insights into Mycobacterium tuberculosis DevR-dependent transcriptional machinery utilizing Escherichia coli

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Saurabh Sharma, Ramesh Kumar, Ayushi Jain, Manoj Kumar, Rahul Gauttam, Rajdeep Banerjee, Jayanta Mukhopadhyay, Jaya Sivaswami Tyagi
Journal: Biochemical Journal
Biochem J (2021) 478 (16): 3079–3098.
https://doi.org/10.1042/BCJ20210268
Published: 20 August 2021
...Saurabh Sharma; Ramesh Kumar; Ayushi Jain; Manoj Kumar; Rahul Gauttam; Rajdeep Banerjee; Jayanta Mukhopadhyay; Jaya Sivaswami Tyagi DevR/DosR response regulator is believed to participate in virulence, dormancy adaptation and antibiotic tolerance mechanisms of Mycobacterium tuberculosis...
View articletitled, Functional insights into Mycobacterium tuberculosis DevR-dependent transcriptional machinery utilizing Escherichia coli
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Includes: Supplementary data
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Structure-based drug repurposing to inhibit the DNA gyrase of Mycobacterium tuberculosis

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Balasubramani GL, Rinky Rajput, Manish Gupta, Pradeep Dahiya, Jitendra K. Thakur, Rakesh Bhatnagar, Abhinav Grover
Journal: Biochemical Journal
Biochem J (2020) 477 (21): 4167–4190.
https://doi.org/10.1042/BCJ20200462
Published: 09 November 2020
... and extensively drug-resistant strains of Mycobacterium tuberculosis (Mtb) H37Rv, as well as the significant death toll globally, necessitates the development of new and effective drugs to treat TB. In this study, we have employed a drug repurposing approach to address this drug resistance problem by screening...
View articletitled, Structure-based drug repurposing to inhibit the DNA gyrase of Mycobacterium tuberculosis
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Includes: Supplementary data
Articles

Antitoxin autoregulation of M. tuberculosis toxin-antitoxin expression through negative cooperativity arising from multiple inverted repeat sequences

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Izaak N. Beck, Ben Usher, Hannah G. Hampton, Peter C. Fineran, Tim R. Blower
Journal: Biochemical Journal
Biochem J (2020) 477 (12): 2401–2419.
https://doi.org/10.1042/BCJ20200368
Published: 26 June 2020
...-like toxin, and a two-domain antitoxin. In Streptococcus agalactiae , the antitoxin AbiEi negatively autoregulates abiE expression through positively co-operative binding to inverted repeats within the promoter. The human pathogen Mycobacterium tuberculosis encodes four DUF1814 putative toxins, two...
View articletitled, Antitoxin autoregulation of M. tuberculosis toxin-antitoxin expression through negative cooperativity arising from multiple inverted repeat sequences
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Includes: Supplementary data
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The thick waxy coat of mycobacteria, a protective layer against antibiotics and the host's immune system

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Sarah M. Batt, David E. Minnikin, Gurdyal S. Besra
Journal: Biochemical Journal
Biochem J (2020) 477 (10): 1983–2006.
https://doi.org/10.1042/BCJ20200194
Published: 29 May 2020
...Sarah M. Batt; David E. Minnikin; Gurdyal S. Besra Tuberculosis, caused by the pathogenic bacterium Mycobacterium tuberculosis ( Mtb ), is the leading cause of death from an infectious disease, with a mortality rate of over a million people per year. This pathogen's remarkable resilience...
View articletitled, The thick waxy coat of mycobacteria, a protective layer against antibiotics and the host's immune system
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Articles

Phosphatase-defective DevS sensor kinase mutants permit constitutive expression of DevR-regulated dormancy genes in Mycobacterium tuberculosis

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Priyanka Kumari, Sudhir Kumar, Kohinoor Kaur, Umesh Datta Gupta, Sameer Suresh Bhagyawant, Jaya Sivaswami Tyagi
Journal: Biochemical Journal
Biochem J (2020) 477 (9): 1669–1682.
https://doi.org/10.1042/BCJ20200113
Published: 11 May 2020
...Priyanka Kumari; Sudhir Kumar; Kohinoor Kaur; Umesh Datta Gupta; Sameer Suresh Bhagyawant; Jaya Sivaswami Tyagi The DevR–DevS/DosR–DosS two-component system of Mycobacterium tuberculosis , that comprises of DevS sensor kinase and DevR response regulator, is essential for bacterial adaptation...
View articletitled, Phosphatase-defective DevS sensor kinase mutants permit constitutive expression of DevR-regulated dormancy genes in Mycobacterium tuberculosis
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Includes: Supplementary data
Articles

Crystal structure of a hemerythrin-like protein from Mycobacterium kansasii and homology model of the orthologous Rv2633c protein of M. tuberculosis

Open Access
Zhongxin Ma, Jan Abendroth, Garry W. Buchko, Kyle H. Rohde, Victor L. Davidson
Journal: Biochemical Journal
Biochem J (2020) 477 (2): 567–581.
https://doi.org/10.1042/BCJ20190827
Published: 31 January 2020
... in an all-inclusive Read & Publish pilot with Portland Press and the Biochemical Society. iron metalloprotein Mycobacterium tuberculosis protein structure The gene Rv2633c in Mycobacterium tuberculosis ( Mtb ) is of interest as it is up-regulated within minutes of phagocytosis...
View articletitled, Crystal structure of a hemerythrin-like protein from Mycobacterium kansasii and homology model of the orthologous Rv2633c protein of M. tuberculosis
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Articles

The Mycobacterium tuberculosis capsule: a cell structure with key implications in pathogenesis

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Rainer Kalscheuer, Ainhoa Palacios, Itxaso Anso, Javier Cifuente, Juan Anguita, William R. Jacobs, Jr, Marcelo E. Guerin, Rafael Prados-Rosales
Journal: Biochemical Journal
Biochem J (2019) 476 (14): 1995–2016.
https://doi.org/10.1042/BCJ20190324
Published: 18 July 2019
... the Mycobacterium tuberculosis (Mtb) capsule began more than 60 years ago, but the relatively recent development of mycobacterial genetics combined with improved chemical and immunological tools have revealed a more refined view of capsule molecular composition. A glycogen-like α-glucan is the major constituent...
View articletitled, The Mycobacterium tuberculosis capsule: a cell structure with key implications in pathogenesis
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Structural insights into the interaction of helicase and primase in Mycobacterium tuberculosis

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Dhakaram Pangeni Sharma, Ramachandran Vijayan, Syed Arif Abdul Rehman, Samudrala Gourinath
Journal: Biochemical Journal
Biochem J (2018) 475 (21): 3493–3509.
https://doi.org/10.1042/BCJ20180673
Published: 15 November 2018
... the functional conservation despite the low sequence homology of the DnaB-binding domains of DnaGs of eubacteria, we determined the crystal structure of the helicase-binding domain of DnaG from Mycobacterium tuberculosis ( Mt DnaG-CTD) and did so to a resolution of 1.58 Å. We observed the overall structure of Mt...
View articletitled, Structural insights into the interaction of helicase and primase in Mycobacterium tuberculosis
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Includes: Supplementary data
Articles

Structural and functional characterization of the transcriptional regulator Rv3488 of Mycobacterium tuberculosis H37Rv

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Meera Kumari, Ravi Kant Pal, Alok K. Mishra, Sarita Tripathi, Bichitra Kumar Biswal, Kishore K. Srivastava, Ashish Arora
Journal: Biochemical Journal
Biochem J (2018) 475 (21): 3393–3416.
https://doi.org/10.1042/BCJ20180356
Published: 09 November 2018
...Meera Kumari; Ravi Kant Pal; Alok K. Mishra; Sarita Tripathi; Bichitra Kumar Biswal; Kishore K. Srivastava; Ashish Arora Rv3488 of Mycobacterium tuberculosis H37Rv has been assigned to the phenolic acid decarboxylase repressor (PadR) family of transcriptional regulators that play key roles...
View articletitled, Structural and functional characterization of the transcriptional regulator Rv3488 of Mycobacterium tuberculosis H37Rv
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Exploring the structure of glutamate racemase from Mycobacterium tuberculosis as a template for anti-mycobacterial drug discovery

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Sinothai Poen, Yoshio Nakatani, Helen K. Opel-Reading, Moritz Lassé, Renwick C.J. Dobson, Kurt L. Krause
Journal: Biochemical Journal
Biochem J (2016) 473 (9): 1267–1280.
https://doi.org/10.1042/BCJ20160186
Published: 26 April 2016
... recently been proven to be essential in Mycobacterium tuberculosis , the causative organism of tuberculosis, a disease for which new medications are urgently needed. In the present study, we have determined the protein crystal structures of MurI from both M. tuberculosis and Mycobacterium smegmatis...
View articletitled, Exploring the structure of glutamate racemase from Mycobacterium tuberculosis as a template for anti-mycobacterial drug discovery
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Crystal structure of Mycobacterium tuberculosis O 6 -methylguanine-DNA methyltransferase protein clusters assembled on to damaged DNA

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Riccardo Miggiano, Giuseppe Perugino, Maria Ciaramella, Mario Serpe, Dominik Rejman, Ondřej Páv, Radek Pohl, Silvia Garavaglia, Samarpita Lahiri, Menico Rizzi, Franca Rossi
Journal: Biochemical Journal
Biochem J (2016) 473 (2): 123–133.
https://doi.org/10.1042/BJ20150833
Published: 05 January 2016
...Riccardo Miggiano; Giuseppe Perugino; Maria Ciaramella; Mario Serpe; Dominik Rejman; Ondřej Páv; Radek Pohl; Silvia Garavaglia; Samarpita Lahiri; Menico Rizzi; Franca Rossi Mycobacterium tuberculosis O 6 -methylguanine-DNA methyltransferase ( Mt OGT) contributes to protect the bacterial GC-rich...
View articletitled, Crystal structure of Mycobacterium tuberculosis O 6 -methylguanine-DNA methyltransferase protein clusters assembled on to damaged DNA
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Includes: Supplementary data
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Alternative substrates reveal catalytic cycle and key binding events in the reaction catalysed by anthranilate phosphoribosyltransferase from Mycobacterium tuberculosis

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Tammie V. M. Cookson, Alina Castell, Esther M. M. Bulloch, Genevieve L. Evans, Francesca L. Short, Edward N. Baker, J. Shaun Lott, Emily J. Parker
Journal: Biochemical Journal
Biochem J (2014) 461 (1): 87–98.
https://doi.org/10.1042/BJ20140209
Published: 13 June 2014
[email protected] ). The structural co-ordinates reported for Mycobacterium tuberculosis anthranilate phosphoribosyltransferase complexed with anthranilate or anthranilate analogues have been deposited in the PDB under codes 4N5V, 4N8Q, 4N93, 4OWM, 4OWN, 4OWO, 4OWQ, 4OWS, 4OWU and 4OWV. using...
View articletitled, Alternative substrates reveal catalytic cycle and key binding events in the reaction catalysed by anthranilate phosphoribosyltransferase from Mycobacterium tuberculosis
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Mechanistic insight into the reaction catalysed by bacterial type II dehydroquinases

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Claire Coderch, Emilio Lence, Antonio Peón, Heather Lamb, Alastair R. Hawkins, Federico Gago, Concepción González-Bello
Journal: Biochemical Journal
Biochem J (2014) 458 (3): 547–557.
https://doi.org/10.1042/BJ20131103
Published: 28 February 2014
...Claire Coderch; Emilio Lence; Antonio Peón; Heather Lamb; Alastair R. Hawkins; Federico Gago; Concepción González-Bello DHQ2 (type II dehydroquinase), which is an essential enzyme in Helicobacter pylori and Mycobacterium tuberculosis and does not have any counterpart in humans, is recognized...
View articletitled, Mechanistic insight into the reaction catalysed by bacterial type II dehydroquinases
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Includes: Supplementary data
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RipD (Rv1566c) from Mycobacterium tuberculosis : adaptation of an NlpC/p60 domain to a non-catalytic peptidoglycan-binding function

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Dominic Böth, Eva Maria Steiner, Atsushi Izumi, Gunter Schneider, Robert Schnell
Journal: Biochemical Journal
Biochem J (2014) 457 (1): 33–41.
https://doi.org/10.1042/BJ20131227
Published: 10 December 2013
...Dominic Böth; Eva Maria Steiner; Atsushi Izumi; Gunter Schneider; Robert Schnell Enzymes carrying NlpC/p60 domains, for instance RipA and RipB from Mycobacterium tuberculosis , are bacterial peptidoglycan hydrolases that cleave the peptide stems and contribute to cell wall remodelling during cell...
View articletitled, RipD (Rv1566c) from Mycobacterium tuberculosis : adaptation of an NlpC/p60 domain to a non-catalytic peptidoglycan-binding function
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Includes: Supplementary data
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Mycobacterium tuberculosis DNA gyrase possesses two functional GyrA-boxes

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Aurélie Bouige, Amélie Darmon, Jérémie Piton, Mélanie Roue, Stéphanie Petrella, Estelle Capton, Patrick Forterre, Alexandra Aubry, Claudine Mayer
Journal: Biochemical Journal
Biochem J (2013) 455 (3): 285–294.
https://doi.org/10.1042/BJ20130430
Published: 10 October 2013
...Aurélie Bouige; Amélie Darmon; Jérémie Piton; Mélanie Roue; Stéphanie Petrella; Estelle Capton; Patrick Forterre; Alexandra Aubry; Claudine Mayer In contrast with most bacteria which possess two type II topoisomerases (topoisomerase IV and DNA gyrase), Mycobacterium tuberculosis possesses only one...
View articletitled, Mycobacterium tuberculosis DNA gyrase possesses two functional GyrA-boxes
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Crystal structure of hexanoyl-CoA bound to β-ketoacyl reductase FabG4 of Mycobacterium tuberculosis

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Debajyoti Dutta, Sudipta Bhattacharyya, Amlan Roychowdhury, Rupam Biswas, Amit Kumar Das
Journal: Biochemical Journal
Biochem J (2013) 450 (1): 127–139.
https://doi.org/10.1042/BJ20121107
Published: 24 January 2013
...-weight FabG) form a phylogenetically separate group of FabG enzymes. FabG4, an HMwFabG from Mycobacterium tuberculosis , contains two distinct domains, an N-terminal ‘flavodoxintype’ domain and a C-terminal oxoreductase domain. The catalytically active C-terminal domain utilizes NADH to reduce β-oxoacyl...
View articletitled, Crystal structure of hexanoyl-CoA bound to β-ketoacyl reductase FabG4 of Mycobacterium tuberculosis
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Regulation of the intersubunit ammonia tunnel in Mycobacterium tuberculosis glutamine-dependent NAD + synthetase

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Watchalee Chuenchor, Tzanko I. Doukov, Melissa Resto, Andrew Chang, Barbara Gerratana
Journal: Biochemical Journal
Biochem J (2012) 443 (2): 417–426.
https://doi.org/10.1042/BJ20112210
Published: 27 March 2012
...Watchalee Chuenchor; Tzanko I. Doukov; Melissa Resto; Andrew Chang; Barbara Gerratana Glutamine-dependent NAD + synthetase is an essential enzyme and a validated drug target in Mycobacterium tuberculosis (mtuNadE). It catalyses the ATP-dependent formation of NAD + from NaAD + (nicotinic acid...
View articletitled, Regulation of the intersubunit ammonia tunnel in Mycobacterium tuberculosis glutamine-dependent NAD + synthetase
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Structural investigation of inhibitor designs targeting 3-dehydroquinate dehydratase from the shikimate pathway of Mycobacterium tuberculosis

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Marcio V. B. Dias, William C. Snee, Karen M. Bromfield, Richard J. Payne, Satheesh K. Palaninathan, Alessio Ciulli, Nigel I. Howard, Chris Abell, James C. Sacchettini, Tom L. Blundell
Journal: Biochemical Journal
Biochem J (2011) 436 (3): 729–739.
https://doi.org/10.1042/BJ20110002
Published: 27 May 2011
...Marcio V. B. Dias; William C. Snee; Karen M. Bromfield; Richard J. Payne; Satheesh K. Palaninathan; Alessio Ciulli; Nigel I. Howard; Chris Abell; James C. Sacchettini; Tom L. Blundell The shikimate pathway is essential in Mycobacterium tuberculosis and its absence from humans makes the enzymes...
View articletitled, Structural investigation of inhibitor designs targeting 3-dehydroquinate dehydratase from the shikimate pathway of Mycobacterium tuberculosis
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Includes: Supplementary data
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Mycobacterium tuberculosis PtkA is a novel protein tyrosine kinase whose substrate is PtpA

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Horacio Bach, Dennis Wong, Yossef Av-Gay
Journal: Biochemical Journal
Biochem J (2009) 420 (2): 155–162.
https://doi.org/10.1042/BJ20090478
Published: 13 May 2009
...Horacio Bach; Dennis Wong; Yossef Av-Gay In Mycobacterium tuberculosis , signal transduction is mediated by 11 serine/threonine kinases, but no tyrosine kinases have been identified thus far. The protein encoded by the ORF (open reading frame) Rv2232 has been annotated as a member of the HAD...
View articletitled, Mycobacterium tuberculosis PtkA is a novel protein tyrosine kinase whose substrate is PtpA
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Temperature stability of proteins essential for the intracellular survival of Mycobacterium tuberculosis

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Nathan A. Lack, Akane Kawamura, Elizabeth Fullam, Nicola Laurieri, Stacey Beard, Angela J. Russell, Dimitrios Evangelopoulos, Isaac Westwood, Edith Sim
Journal: Biochemical Journal
Biochem J (2009) 418 (2): 369–378.
https://doi.org/10.1042/BJ20082011
Published: 11 February 2009
...Nathan A. Lack; Akane Kawamura; Elizabeth Fullam; Nicola Laurieri; Stacey Beard; Angela J. Russell; Dimitrios Evangelopoulos; Isaac Westwood; Edith Sim In Mycobacterium tuberculosis , the genes hsaD (2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid hydrolase) and nat (arylamine N-acetyltransferase...
View articletitled, Temperature stability of proteins essential for the intracellular survival of Mycobacterium tuberculosis
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Includes: Supplementary data
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Characterization of coenzyme binding and selectivity determinants in Mycobacterium tuberculosis flavoprotein reductase A: analysis of Arg 199 and Arg 200 mutants at the NADP(H) 2′-phosphate binding site

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Muna Sabri, Adrian J. Dunford, Kirsty J. McLean, Rajasekhar Neeli, Nigel S. Scrutton, David Leys, Andrew W. Munro
Journal: Biochemical Journal
Biochem J (2009) 417 (1): 103–114.
https://doi.org/10.1042/BJ20080466
Published: 12 December 2008
...Muna Sabri; Adrian J. Dunford; Kirsty J. McLean; Rajasekhar Neeli; Nigel S. Scrutton; David Leys; Andrew W. Munro Mycobacterium tuberculosis FprA (flavoprotein reductase A) is an NAD(P)H- and FAD-binding reductase that is structurally/evolutionarily related to adrenodoxin reductase. Structural...
View articletitled, Characterization of coenzyme binding and selectivity determinants in Mycobacterium tuberculosis flavoprotein reductase A: analysis of Arg 199 and Arg 200 mutants at the NADP(H) 2′-phosphate binding site
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Includes: Supplementary data
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Trp 359 regulates flavin thermodynamics and coenzyme selectivity in Mycobacterium tuberculosis FprA

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Rajasekhar Neeli, Muna Sabri, Kirsty J. McLean, Adrian J. Dunford, Nigel S. Scrutton, David Leys, Andrew W. Munro
Journal: Biochemical Journal
Biochem J (2008) 411 (3): 563–570.
https://doi.org/10.1042/BJ20071298
Published: 14 April 2008
...Rajasekhar Neeli; Muna Sabri; Kirsty J. McLean; Adrian J. Dunford; Nigel S. Scrutton; David Leys; Andrew W. Munro Mtb ( Mycobacterium tuberculosis ) FprA (flavoprotein reductase A) is an NAD(P)H-dependent FAD-binding reductase that is structurally related to mammalian adrenodoxin reductase...
View articletitled, Trp 359 regulates flavin thermodynamics and coenzyme selectivity in Mycobacterium tuberculosis FprA
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Includes: Supplementary data
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Crystal structure and kinetic study of dihydrodipicolinate synthase from Mycobacterium tuberculosis

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Georgia Kefala, Genevieve L. Evans, Michael D. W. Griffin, Sean R. A. Devenish, F. Grant Pearce, Matthew A. Perugini, Juliet A. Gerrard, Manfred S. Weiss, Renwick C. J. Dobson
Journal: Biochemical Journal
Biochem J (2008) 411 (2): 351–360.
https://doi.org/10.1042/BJ20071360
Published: 27 March 2008
... Mycobacterium tuberculosis ( Mtb -DHDPS) was determined and refined at 2.28 Å (1 Å=0.1 nm) resolution. The asymmetric unit of the crystal contains two tetramers, each of which we propose to be the functional enzyme unit. This is supported by analytical ultracentrifugation studies, which show the enzyme...
View articletitled, Crystal structure and kinetic study of dihydrodipicolinate synthase from Mycobacterium tuberculosis
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Quaternary structure and biochemical properties of mycobacterial RNase E/G

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Mirijam-Elisabeth Zeller, Agnes Csanadi, Andras Miczak, Thierry Rose, Thierry Bizebard, Vladimir R. Kaberdin
Journal: Biochemical Journal
Biochem J (2007) 403 (1): 207–215.
https://doi.org/10.1042/BJ20061530
Published: 13 March 2007
.... To learn more about specific properties of RNase E/G homologues from pathogenic Gram-positive bacteria, a polypeptide comprising the catalytic domain of Mycobacterium tuberculosis RNase E/G (MycRne) was purified and characterized in vitro . In the present study, we show that affinity-purified MycRne has...
View articletitled, Quaternary structure and biochemical properties of mycobacterial RNase E/G
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Characterization of phylogenetically distant members of the adenylate cyclase family from mycobacteria: Rv1647 from Mycobacterium tuberculosis and its orthologue ML1399 from M. leprae

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Avinash R. SHENOY, Nandini P. SREENATH, Mohana MAHALINGAM, Sandhya S. VISWESWARIAH
Journal: Biochemical Journal
Biochem J (2005) 387 (2): 541–551.
https://doi.org/10.1042/BJ20041040
Published: 05 April 2005
...Avinash R. SHENOY; Nandini P. SREENATH; Mohana MAHALINGAM; Sandhya S. VISWESWARIAH Analysis of the genome sequence of Mycobacterium tuberculosis H37Rv has identified 16 genes that are similar to the mammalian adenylate and guanylate cyclases. Rv1647 was predicted to be an active adenylate cyclase...
View articletitled, Characterization of phylogenetically distant members of the adenylate cyclase family from mycobacteria: Rv1647 from Mycobacterium tuberculosis and its orthologue ML1399 from M. leprae
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Mycobacterium tuberculosis DnaA initiator protein: purification and DNA-binding requirements

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Anna ZAWILAK, Agnieszka KOIS, Grażyna KONOPA, Aleksandra SMULCZYK-KRAWCZYSZYN, Jolanta ZAKRZEWSKA-CZERWIŃSKA
Journal: Biochemical Journal
Biochem J (2004) 382 (1): 247–252.
https://doi.org/10.1042/BJ20040338
Published: 10 August 2004
...Anna ZAWILAK; Agnieszka KOIS; Grażyna KONOPA; Aleksandra SMULCZYK-KRAWCZYSZYN; Jolanta ZAKRZEWSKA-CZERWIŃSKA The Mycobacterium tuberculosis oriC (the origin of chromosomal replication) region contains 13 non-perfect DnaA boxes. The M. tuberculosis initiator protein, DnaA, was overexpressed...
View articletitled, Mycobacterium tuberculosis DnaA initiator protein: purification and DNA-binding requirements
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