... immunofluorescence analysis showed that the glycosylation-impaired mutants and deglycosylated SNAT1 were equally capable of expressing on the cell surface. However, l -Gln and 3H-labeled methyl amino isobutyrate (MeAIB) was significantly compromised in N-glycosylation-impaired mutants and deglycosylated SNAT1 when...

... ectodomain in the functionality of the promiscuous plant P4-ATPase ALA2. We identified two N-glycosylated residues, Asn 181 and Asn 231 . Whereas mutation of Asn 231 seems to have a small effect on P4-ATPase complex formation, mutation of evolutionarily conserved Asn 181 disrupts interaction between the two...

... in promoting BMP signal activity. However, the mechanism responsible for the membrane tethering and the biological importance of N-glycosylation of S1 remain largely unknown. In the present study, molecular mapping analysis identified a polycationic segment (amino acids 501–550) in the spacer region required...

...Fernando M. Ruggiero; Aldo A. Vilcaes; Ramiro Iglesias-Bartolomé; José L. Daniotti ST3Gal-II, a type II transmembrane protein, is the main mammalian sialyltransferase responsible for GD1a and GT1b ganglioside biosynthesis in brain. It contains two putative N-glycosylation sites (Asn 92 and Asn 211...

...Heidi L. H. Malaby; William R. Kobertz Type I transmembrane peptides acquire N-linked glycans during and after protein synthesis to facilitate anterograde trafficking through the secretory pathway. Mutations in N-glycosylation consensus sites (NXT and NXS, where X≠P) that alter the kinetics...

... structural features were shared across all sites. Notably, the H antigen is not restricted to particular N-glycosylation sites. Also, the Asn 2635 site, previously designated as unoccupied, was found to be highly glycosylated. The delineation of such varied glycan populations in conjunction with current...

... translocation from storage compartments towards the cell surface. How GLUT4 is retained intracellularly is largely unknown. Previously, aberrant GLUT4 N-glycosylation has been linked to increased basal cell-surface levels, while N-glycosylation-deficient GLUT4 was found to be quickly degraded. As recycling...

... 2011 © The Authors Journal compilation © 2011 Biochemical Society 2011 endomannosidase endoplasmic reticulum-associated degradation (ERAD) free oligosaccharide N-glycosylation protein quality control secretory pathway ERAD [ER (endoplasmic reticulum)-associated degradation...

... Society 2010 detergent-resistant membrane endoplasmic reticulum NF-E2 (nuclear factor-erythroid 2)-related factor 1 (Nrf1) N-glycosylation nuclear envelope topology Nrf1 [NF-E2 (nuclear factor-erythroid 2)-related factor 1], Nrf2 and Nrf3 are CNC (cap'n'collar) bZIP (basic-region leucine...

... to a reduction in their ability to synthesize their essential GPI (glycosylphosphatidylinositol) anchors. The RNAi-induced parasites also showed aberrant N-glycosylation of their major cell-surface glycoprotein, variant surface glycoprotein, with loss of the high-mannose Man 9 GlcNAc 2 N-glycosylation at Asn 428...

...Yaowu He; Andrew J. Ramsay; Melanie L. Hunt; Astrid K. Whitbread; Stephen A. Myers; John D. Hooper The Tweety proteins are a family of recently identified putative Cl − channels predicted to be modified by N-glycosylation and, controversially, to contain five or six membrane-spanning domains...

... affected the ABCG2 F208S and S441N variants in terms of both protein levels and intracellular distribution. Immunoblot analysis revealed that those variants were N-glycosylated; however, their oligosaccharides were immature compared with those present on ABCG2 WT. The ABCG2 F208S and S441N variant proteins...

... and therefore they represent good drug targets for the treatment of inflammatory disorders and other pathologies involving selectins. In the present study, we examined the importance of N-glycosylation for the ability of C2GnT-I and FucT-VII to generate functional selectin ligands, particularly the PSGL-1 (P...

... that increases the carbon-dioxide-carrying capacity of blood. To study the topology of TMs (transmembrane segments) 1–4, a series of scanning N-glycosylation mutants were created spanning the region from EC (extracellular loop) 1 to EC2 in full-length AE1. These constructs were expressed in HEK-293 (human...

..., Lass5 and Lass6 were N-glycosylated, each at their N-terminal Asn residue. The occurrence of N-glycosylation of some Lass proteins provides topological insight, indicating that the N-termini of Lass family members probably face the luminal side of the endoplasmic reticulum membrane. Furthermore, based...

...Pascal M. Lanctot; Patrice C. Leclerc; Martin Clément; Mannix Auger-Messier; Emanuel Escher; Richard Leduc; Gaétan Guillemette GPCRs (G-protein-coupled receptors) are preferentially N-glycosylated on ECL2 (extracellular loop 2). We previously showed that N-glycosylation of ECL2 was crucial for cell...

... activity and as a consequence accumulates oligomannosidic structures. Molecular cloning of cgl GnTI cDNA revealed a point mutation, which causes a critical amino acid substitution (Asp 144 →Asn), thereby creating an additional N-glycosylation site. Heterologous expression of cgl GnTI in insect cells...

...Jing ZHU; Itaru WATANABE; Amanda POHOLEK; Matthew KOSS; Barbara GOMEZ; Chaowen YAN; Esperanza RECIO-PINTO; William B. THORNHILL N-glycosylation is a post-translational modification that plays a role in the trafficking and/or function of some membrane proteins. We have shown previously that N...

... demonstration that a specific N-glycan plays a definitive role in mucin dimer formation. Key words: calreticulin, dimer, mucus glycoprotein, N-glycosyl- ation, proteasomal inhibitor. INTRODUCTION Located near the C-terminus of most secretory mucins is a 90 100-residue motif termed the cystine knot (CK) [1 8...

...-type Dol-P-Man: Man ( GlcNAc # -PP-Dol mannosyltransferase cDNA in patient s fibroblasts normalized the mannosyltransferase activity. Key words: inherited disorder, mannosyltransferase, microceph- aly, muscular hypotonia, N-glycosylation. orthologue of the yeast asparagine-linked glycosylation (ALG)12...

...-regulated pro- tein 78), a protein marker of the unfolded protein response INTRODUCTION The normal process of N-glycosylation corresponds to the transfer en bloc of the Glc $ Man * GlcNAc # oligosaccharide chain on an asparagine residue of a nascent protein. This process takes place in the endoplasmic...

... and tetramerization it is possible to generate a rHuAChE displaying a circulatory residence exceeding that of all other known forms of native or recombinant human AChE. The Biochemical Society, London © 2001 2001 MALDITOF-MS N-glycosylation oligomerization pharmacokinetics Biochem. J. (2001) 354, 613...

..., London © 2000 2000 methotrexate resistance membrane protein N-glycosylation protein degradation Biochem. J. (2000) 346, 509 518 (Printed in Great Britain) 509 Mutations in the reduced-folate carrier affect protein localization and stability Heather SADLISH, Richard C. MURRAY, Frederick M. R...

... spectrometry N-glycosylation O-glycosylation Biochem. J. (1999) 343, 645 652 (Printed in Great Britain) 645 Structural characterization of human and bovine lung surfactant protein D Rikke LETH-LARSEN*, Uffe HOLMSKOV‹ and Peter HØJRUP*1 *Institute of Molecular Biology, University of Southern Denmark...

..., N-glycosylation and oligosaccharide processing. Secretion of HL was inhibited by carbonyl cyanide m -chlorophenylhydrazone (CCCP), monensin, brefeldin A (BFA), tunicamycin, castanospermine and N -methyldeoxynojirimycin, but not by 1-deoxymannojirimycin. Secretion of α 1 -antitrypsin, an unrelated N...