... lysis buffer was obtained from Merck Millipore. 30 3 2017 3 7 2017 20 7 2017 21 7 2017 © 2017 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2017 irisin secretion N-linked glycosylation protein stabilization protein...

... system b 0,+ impair its biogenesis, causing cystinuria. We show that a specific N-glycan and the C-terminal disulfide loop of the helper subunit are key elements for biogenesis of system b 0,+ . cystinuria endoplasmic reticulum folding N-linked glycosylation trafficking transporter...

... been shown to undergo N-linked glycosylation, and the N-glycosylation has been shown to be required for membrane trafficking and possibly function. In this study, recombinant wild-type (WT) and glycosylation-defective N380Q HCN2 channels were individually or co-expressed in HEK-293 cells. We...

.../cshperspect.a007526 21875985 TARP γ-8 regulates the surface delivery and channel gating of AMPA receptors. We have identified N-glycosylation sites on TARP γ-8 and demonstrated that the maturation of TARP γ-8 is required for surface expression of GluA1. AMPA receptor CNIH-2 N-linked glycosylation...

...Young-Woong Kim; Jinah Park; Hyun-Ju Lee; So-Young Lee; Seong-Jin Kim N-linked glycosylation is a critical determinant of protein structure and function, regulating processes such as protein folding, stability and localization, ligand–receptor binding and intracellular signalling. TβRII [type II...

... cell lines (J774.2 and RAW264.7 cells). In particular we find that glucose induces these effects by promoting an autocrine activation of Wnt signalling that is mediated by the hexosamine pathway and changes in N-linked glycosylation of proteins. These studies reveal that the Wnt/β-catenin system...

... leptin receptor (Ob-R) N-linked glycosylation peptide N-glycosidase F 2 To whom correspondence should be addressed, at The Scripps Research Institute, Mem-175, 10550 North Torrey Pines Road, La Jolla, CA 92037, U.S.A. (email [email protected] ). 17 8 2007 29 10 2007 6 11...

... detected. Pharmacological inhibition of N-linked glycosylation by tunicamycin in CRT-NN-expressing cells gave a similar reduction in molecular mass, corroborating the finding that Asn 192 and Asn 197 are major N-glycosylation sites in CRT. Although the apparent K m was not significantly affected...

... six times, permitting forma- tion of a channel, which is consistent with its proposed role as a copper transporter. Key words: copper transport, membrane topology, N-linked glycosylation, oligomerization, solute carrier. 35 kDa, which is expressed ubiquitously [12]. Between different cell types...

... 2002 cell surface exposure copper transport solute carrier N-linked glycosylation perinuclear compartment Abbreviations used: BFA, Brefeldin A; CTR1, copper transporter 1; Cy™3, indocarbocyanine; Endo H, endoglycosidase H; FAH, fumaryl acetoacetate hydrolase; FAH/6His, FAH tagged...