...William C. Hallows; Brittany N. Albaugh; John M. Denu Sirtuins are NAD + -dependent enzymes that have been implicated in a wide range of cellular processes, including pathways that affect diabetes, cancer, lifespan and Parkinson's disease. To understand their cellular function in these age-related...

...Claire Ceni; Nathalie Pochon; Michel Villaz; Hélène Muller-Steffner; Francis Schuber; Julie Baratier; Michel De Waard; Michel Ronjat; Marie-Jo Moutin cADPR (cADP-ribose), a metabolite of NAD + , is known to modulate intracellular calcium levels and to be involved in calcium-dependent processes...

...Santina Bruzzone; Iliana Moreschi; Lucrezia Guida; Cesare Usai; Elena Zocchi; Antonio De Flora β-NAD + e (extracellular β-NAD + ), present at nanomolar levels in human plasma, has been implicated in the regulation of [Ca 2+ ] i (the intracellular calcium concentration) in various cell types...

... L -arginine or poly( L -arginine) as the substrate. For the L -arginine assay, each membrane fraction (5 μl) was incubated with 50 mM Tris/HCl (pH 9.0), 5 mM NAD + and 0.1 M L -arginine at 37 °C for 1 h in a total volume of 100 μl. After the reaction was terminated by adding 100 μl of 0.2% SDS...

...Mathias ZIEGLER; Marc NIERE NAD + and its metabolites serve important functions in intracellular signalling. NAD + -mediated regulatory processes also take place on the cell surface, particularly of immune cells. In this issue of the Biochemical Journal , Gerth et al. have demonstrated a new...

...Anja GERTH; Karen NIEBER; Norman J. OPPENHEIMER; Sunna HAUSCHILDT Ca 2+ ions play a critical role in the biochemical cascade of signal transduction pathways, leading to the activation of immune cells. In the present study, we show that the exposure of freshly isolated human monocytes to NAD...

... NAD + -glycohydrolase activity, CD38 is also able to synthesize cyclic ADP-ribose, an endogenous calcium-regulating molecule, from NAD + . In the present study, we have compared ADP-ribosyl cyclase and NAD + -glycohydrolase activities in protein extracts of brains from developing and adult wild-type...

...Richard GRAEFF; Hon Cheung LEE cADP-ribose (cADPR) is a novel cyclic nucleotide derived from NAD + that has now been established as a general Ca 2+ messenger in a wide variety of cells. Despite the obvious importance of monitoring its cellular levels under various physiological conditions, its...

... Cainduced Ca#+ release [18 20]. It would therefore be of interest to test whether the concentration of cADP-ribose increases after stimulation of the Ang II receptor. Membrane-bound and cytosolic ADP-ribosyl cyclases that synthesize cADP-ribose from NAD+ are detected in mammalian tissue [11], including...

...Angélique AUGUSTIN; Hélène MULLER-STEFFNER; Francis SCHUBER Bovine spleen ecto-NAD + glycohydrolase, an archetypal member of the mammalian membrane-associated NAD(P) + glycohydrolase enzyme family (EC 3.2.2.6), displays catalytic features similar to those of CD38, i.e. a protein originally...