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Keywords: NMR spectroscopy
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Biochem J (2022) 479 (5): 687–700.
Published: 16 March 2022
... License 4.0 (CC BY) . circular dichroism NMR spectroscopy peptides phosphorylation/dephosphorylation protein–protein interactions The determinants of α-helix stability are key in understanding the formation and strength of α-helix mediated protein–protein interactions (PPIs) [ 1...
Includes: Supplementary data
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Biochem J (2021) 478 (14): 2871–2887.
Published: 23 July 2021
... 5 2021 18 6 2021 29 6 2021 30 6 2021 © 2021 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2021 electron transfer Geobacter multiheme cytochrome c NMR spectroscopy site-directed mutagenesis Geobacter bacteria...
Includes: Supplementary data
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Biochem J (2021) 478 (5): 1009–1021.
Published: 04 March 2021
... ) 30 11 2020 15 1 2021 19 1 2021 19 1 2021 © 2021 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2021 chemokine glycosaminoglycans heparin heterodimer isothermal titration calorimetry NMR spectroscopy Chemokines...
Includes: Supplementary data
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Biochem J (2021) 478 (1): 197–215.
Published: 15 January 2021
... facilitating the calcium-sensitive interaction with membrane surfaces. In this work, we determined the calcium-free and calcium-bound structures of the dysferlin C2A domain using NMR spectroscopy and X-ray crystallography. We show that binding two calcium ions to this domain reduces the flexibility of the Ca 2...
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Biochem J (2020) 477 (22): 4383–4395.
Published: 26 November 2020
... the Creative Commons Attribution License 4.0 (CC BY) . glycoside hydrolase NMR spectroscopy protein–ligand docking small molecule activators Tr Bgl2 The depletion of fossil fuel in combination with the increasing demand for energy worldwide has stimulated research on alternative...
Includes: Supplementary data
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Biochem J (2020) 477 (17): 3147–3165.
Published: 04 September 2020
... with distinct glycoconjugates and protein counter-receptors in the cytoplasm and nucleus, as well as on the cell surface. Its structural analysis by NMR spectroscopy detected doubling of a set of particular resonances, an indicator of Gal-7 existing in two conformational states in slow exchange on the chemical...
Includes: Supplementary data
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Biochem J (2020) 477 (12): 2193–2219.
Published: 22 June 2020
... ) 15 4 2020 29 5 2020 29 5 2020 1 6 2020 © 2020 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2020 host–pathogen interactions NMR spectroscopy post-translational modification Ubiquitins Ubiquitin (Ub), a 76 residue...
Includes: Supplementary data
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Biochem J (2020) 477 (9): 1613–1630.
Published: 05 May 2020
... [ 13 C, 15 N]-KTI55 SS1448 , respectively. © 2020 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2020 bacterial receptor Gram-positive bacteria NMR spectroscopy plasminogen protein interactions protein–ligand binding M-proteins...
Includes: Supplementary data
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Biochem J (2019) 476 (11): 1679–1694.
Published: 14 June 2019
... domains of SPAG1 in the recruitment of HSP chaperones by combining biochemical assays, ITC, NMR spectroscopy and molecular dynamics (MD) simulations. First, we propose that only two, out of the three TPR domains, are able to recruit the protein chaperones HSP70 and HSP90. We then focused on one...
Includes: Multimedia, Supplementary data
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Biochem J (2019) 476 (3): 613–628.
Published: 14 February 2019
... 25 1 2019 © 2019 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2019 conformational heterogeneity endolysins NMR spectroscopy To accomplish biological functions, proteins must conserve their native state with a considerable degree...
Includes: Supplementary data
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Biochem J (2018) 475 (14): 2377–2393.
Published: 31 July 2018
.... Using a combined approach based on NMR spectroscopy, synthesis and in vitro testing of all Ala-scan mutants of the peptide and molecular docking/molecular dynamics, we have generated a detailed model of the AIF (370–394)/CypA complex. The model suggests us that the central region of the peptide spanning...
Includes: Supplementary data
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Biochem J (2018) 475 (12): 2043–2055.
Published: 26 June 2018
... to facilitate robust and reliable identification of compounds that occupy either YAP-binding pocket or palmitate-binding pocket. Here, using NMR spectroscopy, we validated compounds that bind to these pockets and also identify the residues in mouse TEAD4 (mTEAD4) that interact with these compounds. Flufenamic...
Includes: Supplementary data
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Biochem J (2018) 475 (7): 1371–1383.
Published: 16 April 2018
... License 4.0 (CC BY) . enzymology metabolism mutarotase NMR spectroscopy sulfoglycolysis Various prokaryotes metabolize the sugar sulfoquinovose (SQ) to sulfolactaldehyde (SLA) and dihydroxyacetone phosphate (DHAP), via an Embden–Meyerhof–Parnas (EMP)-like pathway [ 1 ], or pyruvate, via...
Includes: Supplementary data
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Biochem J (2017) 474 (19): 3321–3338.
Published: 25 September 2017
.... 14 6 2017 9 8 2017 15 8 2017 15 8 2017 © 2017 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2017 HIV-1 reverse transcriptase metamorphic NMR spectroscopy structural maturation Despite the substantial progress that has been...
Includes: Supplementary data
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Biochem J (2017) 474 (19): 3307–3319.
Published: 20 September 2017
..., identify by NMR spectroscopy and confirm by docking simulations, a new but cryptic phosphoinositide-binding site, comprising contiguous A1, A1′ and B helices. The addition of helix A1′, unusual among RhoGAP domains, seems to be crucial for lipid interactions. Such a site was totally unexpected inside...
Includes: Supplementary data
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Biochem J (2017) 474 (11): 1853–1866.
Published: 16 May 2017
... ) 1 12 2016 11 4 2017 13 4 2017 13 4 2017 © 2017 The Author(s); published by Portland Press Limited on behalf of the Biochemical Society 2017 Alzheimer's disease calcium signaling co-chaperone NMR spectroscopy tetratricopeptide repeat The S100 family...
Includes: Supplementary data
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Biochem J (2016) 473 (20): 3451–3462.
Published: 11 October 2016
... The Author(s); published by Portland Press Limited on behalf of the Biochemical Society 2016 giant virus glycogen glycosylation NMR spectroscopy polysaccharide ANOVA with multiple comparisons was used and P  < 0.05 was considered statistically significant. GraphPad Prism 6 software...
Includes: Supplementary data
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Biochem J (2016) 473 (19): 3113–3126.
Published: 27 September 2016
... the wolf spider Alopecosa marikovskyi (Lycosidae), studied by NMR spectroscopy. PT2 is composed of an N-terminal inhibitor cystine knot (ICK, or knottin) β-structural domain and a C-terminal linear cationic domain. In aqueous solution, the C-terminal fragment is hyper-flexible, whereas the knottin domain...
Includes: Supplementary data
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Biochem J (2016) 473 (8): 1085–1095.
Published: 08 April 2016
... at the C-terminus. In order to obtain insights into the mechanism of chitosan recognition, the structures of DD1 and DD2 were solved by NMR spectroscopy and crystallography. DD1 and DD2 both adopted a β-sandwich fold with several loops in solution as well as in crystals. On the basis of chemical shift...
Includes: Supplementary data
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Biochem J (2016) 473 (8): 1097–1110.
Published: 08 April 2016
... substrate-mimics, some 4′-phosphopantetheine prosthetic group arms swing freely, whereas others stick to the protein surface, suggesting a possible mode of interaction with enzyme domains during polyketide biosynthesis. acyl carrier protein mycolactone NMR spectroscopy 4′-phosphopantetheine type I...
Includes: Supplementary data
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Biochem J (2013) 456 (3): 397–407.
Published: 22 November 2013
... to be properly defined. antibiotic resistance enzyme structure inhibitor binding metallo-β-lactamase NMR spectroscopy zinc enzyme The rapid increase in resistance to β-lactam antibiotics is a major clinical and public health concern, as these antibiotics have long been key to the treatment...
Includes: Supplementary data
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Biochem J (2013) 454 (3): 459–466.
Published: 29 August 2013
... Society 2013 microtubule-associated protein light chain 3 beta (LC3) NMR spectroscopy optineurin protein–protein interaction selective autophagy X-ray crystallography Autophagy is a process by which cells manage to deliver bulky cellular substrates, such as aggregates or damaged...
Includes: Supplementary data
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Biochem J (2013) 449 (2): 415–425.
Published: 14 December 2012
... (24 scans, 160 increments) was recorded both directly after this addition and on the following day after overnight storage of the NMR sample at 4°C. binding-induced folding lipoate protein ligase lipoyl domain NMR spectroscopy protein–protein interaction X-ray crystallography Aerobic...
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Biochem J (2012) 445 (2): 167–174.
Published: 27 June 2012
...) [ 23 , 24 ] indicated that the secondary structure of HIP2 contains four α-helices and four β-sheets, consistent with the X-ray crystal structures. catalytic pocket hydrogen bonding NMR spectroscopy structure ubiquitin E2 conjugating enzymes are central components in the Ub (ubiquitin...
Includes: Supplementary data
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Biochem J (2011) 434 (1): 37–48.
Published: 27 January 2011
.... annexin calcium-binding protein EF-hand NMR spectroscopy phospholipid-binding protein S100A11 The S100 proteins comprise a group of 25 members within the EF-hand calcium-binding protein family. Most of these dimeric proteins have been shown to act as calcium-signalling molecules, in a manner...
Includes: Supplementary data
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Biochem J (2010) 425 (3): 513–522.
Published: 15 January 2010
.... cruzi glutathione peroxidase-like enzyme I). We also characterized the wild-type, C48G and C96G variants of TcGPXI by NMR spectroscopy and biochemical assays. Our results show that residues Cys 48 and Cys 96 are required for catalytic activity. In solution, the TcGPXI molecule readily forms a Cys 48...
Includes: Supplementary data
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Biochem J (2009) 422 (2): 207–215.
Published: 13 August 2009
... Intrinsically disordered protein NMR spectroscopy prokaryote protein degradation Pup ubiquitin In bacteria, protein degradation is carried out by four families of energy-dependent proteases including ClpAP/XP, HslUV/ClpQY, Lon and FtsH [ 8 ]. In all of them, substrates are first recognized...
Includes: Supplementary data
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Biochem J (2008) 413 (2): 281–290.
Published: 26 June 2008
... 2008 Biochemical Society 2008 Bet v 1 allergen homology modelling norcoclaurine synthase NMR spectroscopy substrate binding BIAs (benzylisoquinoline alkaloids) form an important group of plant secondary metabolites including approx. 2500 different compounds. Many of these small...
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Biochem J (2008) 411 (3): 571–579.
Published: 14 April 2008
... 2008 Biochemical Society 2008 Bacillus subtilis copper-mediated dimerization copper trafficking cysteine thiol luminescence NMR spectroscopy Copper is an essential metal ion involved in a wide variety of cellular processes, including oxygen transport, electron transfer, respiration...
Includes: Supplementary data
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Biochem J (2008) 411 (3): 553–561.
Published: 14 April 2008
... © 2008 Biochemical Society 2008 acetylation high mobility group (HMG) box NMR spectroscopy non-histone protein site-directed mutagenesis Protein acetylation plays a critical role in the control of gene transcription. This is effected through modulation of chromatin packaging...
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Biochem J (2007) 401 (2): 465–473.
Published: 21 December 2006
... glucose synthesis glutamine metabolism ketone body NMR spectroscopy small intestine The contribution of the liver to glucose production in normal fasted humans and animals is well established; under this situation, the liver produces glucose as a result of both gluconeogenesis mainly from...
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Biochem J (2006) 399 (2): 191–198.
Published: 27 September 2006
... in AutoDockTools program. We wrote a specific Python script program to allow detection and systematic monitoring of contacts between side-chain polar groups of the protein and selected defined ligand atoms. antithrombin conformation docking heparin NMR spectroscopy protein–carbohydrate interaction...
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Biochem J (2004) 384 (1): 93–99.
Published: 09 November 2004
...–peptide complexes were prepared by direct addition of several aliquots of a concentrated solution of unlabelled sugar (typically 15 mM) to the NMR peptide sample. heparin-derived oligosaccharide interferon-γ (IFNγ) NMR spectroscopy protein–carbohydrate interaction NMR spectra were recorded...
Includes: Supplementary data
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Biochem J (2002) 368 (2): 483–494.
Published: 01 December 2002
...-glycans of previously established structure accounted for 13% of total glycosylation. The remainder could be attributed to a peptidoglycan-associated secondary cell wall polymer. Structure analysis was performed using purified secondary cell wall polymer—peptidoglycan complexes. NMR spectroscopy revealed...
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Biochem J (2002) 364 (3): 725–737.
Published: 15 June 2002
... been associated with fatty acid signalling, cell growth, regulation and differentiation. As a contribution to understanding the structure—function relationship, we report in the present study features of its solution structure and backbone dynamics determined by NMR spectroscopy. Applying multi...
Includes: Supplementary data
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Biochem J (2001) 359 (2): 265–272.
Published: 08 October 2001
...-glycoside of A respectively) glycosidic linkages. Evidence is also provided that the protein drives the conformation of the 2-O-sulphated iduronic acid residue towards the skewed 2 S 0 form. NMR spectroscopy protein–carbohydrate interaction solution structure Abbreviations used: 3D, three...
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Biochem J (2001) 354 (2): 259–266.
Published: 22 February 2001
... solution. The binding of fatty acid molecules to FABPs, which proceeds through a portal region on the protein surface, is of particular interest. In the present study we have determined the three-dimensional solution structure of human heart-type FABP by multi-dimensional heteronuclear NMR spectroscopy...
Includes: Supplementary data
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Biochem J (2000) 348 (3): 649–656.
Published: 07 June 2000
... neurotoxin I). Although DLPs are the major peptides in the platypus venom, little is known about their biological roles. In this study, we determined the three-dimensional structure of DLP-2 by NMR spectroscopy, with the aim of gaining insights into the natural function of the DLPs in platypus venom. The DLP...
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Biochem J (2000) 346 (2): 385–391.
Published: 22 February 2000
... (‘MFBAPTA’), with dissociation constants for Ca 2+ ranging from 46 to 537 nM, have been used to measure [Ca 2+ ] i , over the range from less than 100 nM to more than 3 μ M, in Langendorff-perfused ferret hearts (30 °C, pH 7.4, paced at 1.0 Hz) by 19 F-NMR spectroscopy. Loading hearts with indicators...
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Biochem J (1999) 344 (2): 495–501.
Published: 24 November 1999
... 1999 The Biochemical Society, London © 1999 1999 fluorescence NMR spectroscopy portal region site-directed mutagenesis Biochem. J. (1999) 344, 495 501 (Printed in Great Britain) 495 Functional and conformational characterization of new mutants of heart fatty acid-binding protein Aukje...
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