.... Neuroendocrine PC12 cell lines stably expressing wild type or L115R ANCL mutant CSP constructs fused to miniTurbo were generated; then the biotinylated proteomes were analysed by liquid chromatography–mass spectrometry and validated by western blotting. This confirmed several known CSP-interacting proteins...

...). Differentiation of PC12 cells was also associated with the up-regulation of adenosine A 1 receptors. Addition of adenosine receptor agonists to cell cultures increased CNT2-related activity by a mechanism consistent with A 1 and A 2A receptor activation. The addition of adenosine to the culture medium also...

... interfaced to an Intel Pentium II system running the Leica TCS NT control software. Parental PC12 cells (ECACC accession number 88022401) were cultured in DMEM (Dulbecco's modified Eagle's medium) supplemented with 5% foetal bovine serum and 10% horse serum. Prior to stimulation, cells were serum...

...: Institute of Cell Biology, University of Berne, Baltzerstrasse 4, CH-3012 Berne, Switzerland. 6 4 2006 16 6 2006 23 6 2006 23 6 2006 The Biochemical Society, London 2006 Akt PC12 cell FYVE domain 3T3-L1 adipocyte vesicle WD-repeat WD (tryptophan-aspartic acid...

... a role in differentiation, its function remains unknown. We established Tet-off inducible expression of Pcp2 in PC12 cells (rat pheochromocytoma cells) to determine whether Pcp2 regulates neuronal differentiation. Utilizing a polyclonal antibody, Pcp2 was localized in the cell body and throughout...

... specifically with syntaxin 1, a 35 kDa membrane protein, to mediate the fusion process. Here, we investigated whether other known syntaxin isoforms found at the plasma membrane can serve as binding partners for SNAP-25 in vivo . In our analysis, we employed rat phaeochromocytoma PC12 cells that are often used...

..., the components of the complexes and their physical properties at endogenous expression levels have not been defined. We characterized a large protein complex containing a nerve-growth-factor-activated ERK (extracellular-signal-regulated kinase) and MEK (MAPK/ERK kinase) in rat pheochromocytoma (PC12) cells...

...Mitsunori FUKUDA Although PC12 cells express three synaptotagmin isoforms (Syts I, IV and IX), all of which have been proposed to regulate dense-core vesicle exocytosis, it remains unknown which of the Sytisoforms acts as the major Ca 2+ sensor for dense-core vesicle exocytosis. In the present...

...James MWANJEWE; Ashok K. GROVER Cells take up transferrin-bound iron or NTBI (non-transferrin-bound iron). After treatment with NGF (nerve growth factor), PC12 cells exhibited a neuronal phenotype and an increase in the NTBI uptake ( 55 Fe 2+ or 55 Fe 3+ ). We loaded the cells with the dye calcein...