... to this work. 2 To whom correspondence should be addressed (email [email protected] ). acute myeloid leukaemia all- trans -retinoic acid (ATRA) CD11b differentiation PMA PU.1 Haemopoiesis is driven and co-ordinated by a precise cascade of events governed by the specific temporal...

... study, we report the stimulatory effect of PMA on the hNHE3 (human NHE3) transcription. Pretreatment with actinomycin D or cycloheximide blocked the up-regulation of the NHE3 mRNA by PMA, indicating that the increased level of NHE3 mRNA expression is regulated by transcriptional activation...

... and an activator of novel PKC isoforms had no effect. PKC inhibitors (including the PKCα-preferring Ro 31-8280) reduced the inhibitory effect of the PKC-activating compounds. Microscopic analyses revealed a PMA-induced reduction in the cell-surface expression of fusion proteins between hCAT-3 and enhanced green...

... and late endosomes, finally resulting in their endolysosomal degradation. PMA, EGF (epidermal growth factor), bafilomycin A1 and cycloheximide were obtained from Sigma (St. Louis, MO, U.S.A.). Anti-Cx43 antibodies were obtained by injecting rabbits with a synthetic peptide consisting of the 20 C-terminal...

...Hye Jin YOU; Chang-Hoon WOO; Eun-Young CHOI; Sung-Hoon CHO; Yung Joon YOO; Jae-Hong KIM The roles of Rac and p38 kinase in the activation of cPLA 2 (cytosolic PLA 2 ) in Rat-2 fibroblasts were investigated. In the present study, we found that PMA activates cPLA 2 by a Rac-p38 kinase-dependent...

... (IGF-1), dexamethasone, thyroid hormones and PMA was studied. HepG2 cells were transfected transiently with luciferase reporter gene constructs containing DNA fragments flanking the 5′-region of the human CYP27A1 gene. GH, IGF-1 and dexamethasone increased the promoter activity by 2–3-fold, whereas...

..., but its use as an activator of PLD2 in intact cells to study function is limited due to toxicity. Key words: antigen, exocytosis, membrane ruffling, phosphatidic acid, PMA. Šitro [10]. PLD2 can be activated mildly by ARF, exhibiting 1.5 2-fold activation [11,12] ; however, when the N-terminal residues 1...

.... The α 1 -adrenergic agonist cirazoline also increased CREB phosphorylation. An increase in intracellular [Ca 2+ ] had, however, no effect, but protein kinase C activation by PMA was a potent stimulator. The cirazoline-stimulated (α 1 -adrenergic) CREB phosphorylation was inhibited by a desensitizing...

... by a 24-h treatment with 50ng/ml PMA, an agent that promotes keratinocyte differentiation. Previous studies show that thapsigargin (TGN), an agent that depletes intracellular calcium stores, inhibits keratinocyte differentiation. In the present study we show that TGN inhibits the PMA-dependent...

..., treatment of the cells with PMA, which activates PKC-θ and hence increases the phosphorylation state of MARCKS, reversibly inhibited both MARCKS translocation and myoblast fusion. These results suggest that MARCKS is preferentially phosphorylated by PKC-θ in cultured myoblasts and that the down-regulation...

... of 1,25(OH) 2 D 3 but had no effect on the IFN-γ-mediated increase in activity of sphingomyelinase. Isoform-specific antibodies were used to deplete different PKC isoforms from cytosol before the treatment of the cytosolic fraction with 1,25(OH) 2 D 3 , arachidonic acid (AA) and PMA. The depletion of PKC...