... with the developmental stages of their respective sexes. RNA interference with dsRNA showed that the knockdown of AfrDsx M in male larvae led to the appearance of female traits including an ovary-like structure in the original male reproductive system and an elevated expression of vitellogenin. However, silencing...

... microscopy. For SEM (scanning electron microscope) analysis, cysts from the test and control groups were fixed as described by Dai et al. [ 24 ] and observed under a SEM (Hitachi). apoptosis Artemia cyst RNA interference trehalase trehalose Trehalose, a non-reducing disaccharide...

... renal cell carcinoma (RCC) RNA interference transcriptional regulation Of the three hypoxia-inducible transcription factor family members identified in mammals so far, HIF (hypoxia-inducible factor)-3 is the least characterized and understood. HIF-1 and -2 are heterodimeric transcription...

... and that knockdown of ERα with RNA interference increased slug expression. More specifically, slug expression was down-regulated in ERα-negative MDA-MB-468 cells transfected with ERα after treatment with E2 (17β-oestradiol). The down-regulation of slug in the ERα-positive MCF-7 cell line was mediated by direct...

...Katherine A. Kaproth-Joslin; Xiangquan Li; Sarah E. Reks; Grant G. Kelley In the present study, we examined the role of PLCδ1 (phospholipase C δ1) in the regulation of cellular proliferation. We demonstrate that RNAi (RNA interference)-mediated knockdown of endogenous PLCδ1, but not PLCβ3 or PLCϵ...

... should be addressed (email [email protected] ). 30 1 2007 9 2 2007 14 2 2007 14 2 2007 © The Authors Journal compilation © 2007 Biochemical Society 2007 RNA interference selenocysteine selenocysteine synthesis selenoprotein selenoprotein synthesis...

... glycohydrolase). To overcome the early embryonic lethality of parg -knockout mice and gain more insights into the biological functions of PARG, we used an RNA interference approach. We found that as little as 10% of PARG protein is sufficient to ensure basic cellular functions: PARG-silenced murine and human...

... is phosphorylated by IKKβ upon TNFα stimulation and that this modification negatively regulates TANK binding to NEMO (NF-κB essential modulator). Interestingly, reduced TANK expression by RNA interference attenuates TNFα-mediated induction of a subset of NF-κB target genes through decreased p65 transactivation...

... is synthesized from glutathione and spermidine by a single enzyme, TryS (trypanothione synthetase), with glutathionylspermidine as an intermediate. To examine the physiological roles of trypanothione, tetracycline-inducible RNA interference was used to reduce expression of TRYS . Following induction, TryS...

... with reducing sample buffer for SDS/PAGE and boiled. For immunoblot analysis, syndecan-4 bound to GST–LG4 was detected with anti-syndecan-4 antibody 8G3. RNA interference was carried out essentially as described in [ 35 , 36 ]. Templates for the preparation of ds (double-stranded) RNA were PCR-derived...

... roles of these enzymes in vivo , we report in the present study the effects of decreased expression, using RNA interference, on the response of S2 cells to different stressors. When either of the TPxs was blocked, cells became relatively more susceptible to oxidative stress caused by exposure...

... distribution of all three cathepsin B transcripts and thus resembled subcultured chondrocytes at an early stage of dedifferentiation. Transfection of T/C-28a2 chondrocytes with double-stranded cathepsin B mRNA resulted in inhibition of cathepsin B biosynthesis by up to 70% due to RNA interference, and single...

... and the rate of trypanosome growth was halved. Key words: glycolysis, glycosome, metabolic control, RNA interference, trypanosome alternative oxidase. control of the glycolytic flux is exerted by the plasma-membrane glucose transporter, but some other enzymes also contribute to control [2]. In bloodstream...

...Akira GOTO; Yuichi MATSUSHIMA; Tatsuhiko KADOWAKI; Yasuo KITAGAWA We have cloned cDNA encoding Drosophila mitochondrial (mt) transcription factor A ( d -TFAM). RNA interference (RNAi) of d -TFAM by lipofection of haemocyte-derived Kc167 cells with double-stranded RNA reduced d -TFAM to less than 5...