... regulator in both innate and adaptive immunity by targeting inflammatory mRNA degradation. Here we report that MCPIP1 RNase can also selectively detect and degrade the mRNAs encoded by transfected plasmids. In transient transfection, MCPIP1 expression potently degraded the mRNA from exogenously transfected...

...Elio Pizzo; Antonello Merlino; Mimmo Turano; Irene Russo Krauss; Francesca Coscia; Anna Zanfardino; Mario Varcamonti; Adriana Furia; Concetta Giancola; Lelio Mazzarella; Filomena Sica; Giuseppe D'Alessio Recently, extracellular RNases of the RNase A superfamily, with the characteristic CKxxNTF...

... for further analysis. For fluorescence-microscopy experiments, bovine RNase A (Sigma) was labelled with RITC (rhodamine B isothiocyanate; Sigma) as described previously [ 15 ]. GH3 cells were seeded on to a six-well plate and grown on coverslips at 40000 cells/well for 24 h. mECP–eGFP–6H, eGFP–6H or RITC...

..., dephosphorylation, Rnase. PNUTS [11]. Various other mammalian inhibitory polypeptides of PP1 have been described, including inhibitor-1 and the structurally related protein DARPP-32 [2,12], inhibitor-2 [2], inhibitor-3 [13], sds22 [14], RIPP1 [15] and p53BP2 [16], but these regulators have no established targeting...