... 17 02 2018 © 2025 The Author(s). 2025 This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) . kinase leucine rich repeat kinase phosphorylation Rab...

... inhibitor leucine rich repeat kinase Parkinsons disease Rab GTPase Autosomal dominant mutations that activate the LRRK2 (leucine rich repeat protein kinase-2) signaling pathway cause late-onset Parkinson's disease [ 1 , 2 ]. LRRK2 is a large 2527 residue multidomain enzyme bearing an armadillo...

... mutations in LRRK2 that enhance kinase activity cause Parkinson's disease. LRRK2 phosphorylates a subset of Rab GTPases including Rab8A and Rab10 within its effector binding motif. Here, we explore whether LRRK1, a less studied homolog of LRRK2 that regulates growth factor receptor trafficking...

...Alexia F. Kalogeropulou; Jordana B. Freemantle; Pawel Lis; Edmundo G. Vides; Nicole K. Polinski; Dario R. Alessi Mutations that enhance LRRK2 protein kinase activity cause inherited Parkinson's disease. LRRK2 phosphorylates a group of Rab GTPase proteins, including Rab10 and Rab12, within...

... Parkinson's disease. Recent work has revealed that LRRK2 directly phosphorylates a conserved threonine/serine residue in the effector-binding switch-II motif of a number of Rab GTPase proteins, including Rab10. Here we describe a facile and robust method to assess phosphorylation of endogenous Rab10 in mouse...

..., Sec18p and Sec17p (yeast NSF and α-SNAP) and the HOPS (homotypic fusion and protein sorting)–Class C Vps (vacuole protein sorting) complex, but not the vacuolar Rab GTPase Ypt7p. The present study investigates the activity of Ypt7p in proteoliposome lipid mixing. Ypt7p is required for the lipid mixing...

...Rudi A. Baron; Miguel C. Seabra Prenylation (or geranylgeranylation) of Rab GTPases is catalysed by RGGT (Rab geranylgeranyl transferase) and requires REP (Rab escort protein). In the classical pathway, REP associates first with unprenylated Rab, which is then prenylated by RGGT. In the alternative...