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Biochem J (2005) 387 (3): 737–745.
Published: 26 April 2005
... and that Sp3 (specificity protein 3), a ubiquitously expressed transcription factor, but not Sp1 binds to this GC-rich motif, suggesting that Sp3 is involved in the regulation of SOCS3 expression. The results suggest that Sp3 is important for IL-6-induced transcriptional activation of the SOCS3 (gene...
Biochem J (2005) 385 (2): 557–564.
Published: 07 January 2005
... promoter activities and mRNA expression levels of DNMT3A and DNMT3B . Overexpression of Sp1 and Sp3 up-regulated the promoter activities of these two genes. The physical binding of Sp1 and Sp3 to DNMT3A and DNMT3B promoters was confirmed by a gel shift assay. Interestingly, Sp3 overexpression in HEK-293T...
Biochem J (2004) 382 (3): 975–980.
Published: 07 September 2004
... assays showed that the interaction of proteins in a nuclear extract from rat luteinized granulosa cells with this region was inhibited by a competitor having the wild-type Sp1 sequence in its promoter, but not a mutated Sp1 sequence. Supershift analysis confirmed that Sp1 and Sp3 were present in the...
Biochem J (2004) 380 (3): 735–747.
Published: 15 June 2004
... activated T cells (NFAT) Sp1 Sp3 Abbreviations used: BCA, bicinchoninic acid; C/EBP, CCAAT/enhancer-binding protein; ChiP, chromatin immunoprecipitation; EMSA, electrophoretic mobility-shift assay; IL, interleukin; MC, mesangial cell; (MT1)-MMP, (membrane type 1) matrix metalloproteinase; NFAT...
Biochem J (2004) 378 (2): 473–484.
Published: 01 March 2004
... basal transcription, but only limited induction by TRH and EGF. Site-directed mutagenesis of the reporter construct combined with band-shift and in vivo studies revealed that part of the constitutive activity of the MKP-1 promoter resides in two GC boxes bound by Sp1 and Sp3 transcription factors in the...
Biochem J (2003) 373 (3): 925–932.
Published: 01 August 2003
... the identification of three Sp1/Sp3-binding sites within this region, two of which are absolutely required both for promoter function and cell-type-specific activity. By Western blotting a panel of expressing and non-expressing breast tumour lines we show that the latter have higher levels of Sp3...
Biochem J (2003) 371 (2): 265–275.
Published: 15 April 2003
...'), CCAAT/enhancer-binding protein ('C/EBP') and E2F transcription factors. In one case, the boxes for Sp1 and NF-Y are overlapping. Gel-shift and supershift assays demonstrated specific binding of Sp1, Sp3 and NF-Y proteins. Transient transfections and luciferase assays revealed activation of the Sp1...
Milota KALUZOVÁ, Silvia PASTOREKOVÁ, Eliska SVASTOVÁ, Jaromír PASTOREK, Eric J. STANBRIDGE, Stefan KALUZ
Biochem J (2001) 359 (3): 669–677.
Published: 25 October 2001
... HeLa nuclear extracts. Of these, three were completely competed with the SP1 and transforming growth factor-β retinoblastoma control-element CACCC box (RCE) probes, whereas the AP2 probe competed against the same three complexes partially. Supershift EMSA identified SP1 in the complex 1 and SP3 in the...