..., but nothing is known about the relationship between trpF-3 and priA . After in vitro steady-state enzyme kinetics we found that trpF-3 encodes a phosphoribosyl anthranilate isomerase. However, mutation of this gene in Streptomyces sviceus did not lead to auxothrophy, as expected from the biosynthetic role...

...Katie L. I. M. Blundell; Michael A. Hough; Erik Vijgenboom; Jonathan A. R. Worrall In Streptomyces lividans an extracytoplasmic copper-binding Sco protein plays a role in two unlinked processes: (i) initiating a morphological development switch and (ii) facilitating the co-factoring of the Cu...

... family 2 (GH2). Sequence alignments identified a group of CsxA-related protein sequences forming a distinct GH2 subfamily. Most of them have been annotated in databases as putative β-mannosidases. Among these, the SAV1223 protein from Streptomyces avermitilis has been purified following gene cloning...

... be used to comprehensively evaluate the reactions that result in the formation both of D -Ala from L -Ala and of L -Ala from D -Ala at several substrate concentrations. Streptomyces ALR also catalyses the formation of D -Ser from L -Ser and of L -Ser from D -Ser, but the catalytic constants ( k cat...

... of Streptomyces coelicolor were overexpressed in Escherichia coli and the DmdR1 and DmdR2 proteins were purified to homogeneity. Electrophoretic mobility-shift assays showed that both DmdR1 and DmdR2 bind to the 19-nt tox and desA iron boxes forming two different complexes in each case. Increasing...

...David C. LAMB; Kay FOWLER; Tobias KIESER; Nigel MANNING; Larissa M. PODUST; Michael R. WATERMAN; Diane E. KELLY; Steven L. KELLY The annotation of the genome sequence of Streptomyces coelicolor A3(2) revealed a cytochrome P450 (CYP) resembling various sterol 14α-demethylases (CYP51). The putative...