... This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) . human Leishmania phosphofructokinase reverse reaction Trypanosoma cruzi Trypanosoma brucei...

... that the motif is not involved in binding to PIGO or PIGG, but critical for its function. Finally, we identified a PIGF homologue from Trypanosoma brucei and showed that it binds specifically to the T. brucei PIGO homologue. These data together support the notion that PIGF plays a critical and evolutionary...

... depends on the up-take of exogenous amino acids. Amino acid transporters are also of high pharmacological relevance because they may mediate uptake of toxic amino acid analogues. In the present study we show that the eflornithine transporter AAT6 from Trypanosoma brucei (TbAAT6) mediates growth on neutral...

...Craigen R. Nes; Ujjal K. Singha; Jialin Liu; Kulothungan Ganapathy; Fernando Villalta; Michael R. Waterman; Galina I. Lepesheva; Minu Chaudhuri; W. David Nes Trypanosoma brucei is the protozoan parasite that causes African trypanosomiasis, a neglected disease of people and animals. Co-metabolite...

...Jialin Liu; Kulothungan Ganapathy; Ewa Wywial; Janusz M. Bujnicki; Chizaram A. Nwogwugwu; W. David Nes Tb SMT [ Trypanosoma brucei 24-SMT (sterol C-24-methyltransferase)] synthesizes an unconventional 24-alkyl sterol product set consisting of Δ 24(25) -, Δ 24(28) - and Δ 25(27) -olefins. The C...

...Helen Denton; Stewart Fyffe; Terry K. Smith A putative GDP-Man PP (guanidine diphosphomannose pyrophosphorylase) gene from Trypanosoma brucei ( Tb GDP-Man PP) was identified in the genome and subsequently cloned, sequenced and recombinantly expressed, and shown to be a catalytically active dimer...

...Bhargavi Patham; Josh Duffy; Ariel Lane; Richard C. Davis; Peter Wipf; Sheara W. Fewell; Jeffrey L. Brodsky; Kojo Mensa-Wilmot HAT (human African trypanosomiasis), caused by the protozoan parasite Trypanosoma brucei , is an emerging disease for which new drugs are needed. Expression of plasma...

...Sandesh Subramanya; C. Frank Hardin; Dietmar Steverding; Kojo Mensa-Wilmot GPI-PLC (glycosylphosphatidylinositol-specific phospholipase C) is expressed in bloodstream-form Trypanosoma brucei , a protozoan that causes human African trypanosomiasis. Loss of genes encoding GPI-PLC reduces...

.... Database mining revealed two putative C/EKs (choline/ethanolamine kinases) in the Trypanosoma brucei genome, which were cloned, overexpressed, purified and characterized. Tb EK1 ( T. brucei ethanolamine kinase 1) was shown to be catalytically active as an ethanolamine-specific kinase, i.e. it had...

... to treat patients with late-stage African trypanosomiasis, the requirement for additional drug targets is paramount. Here, we report the biochemical properties of TbSpSyn ( Trypanosoma brucei spermidine synthase), the enzyme immediately downstream of ODC in this pathway. Recombinant TbSpSyn was purified...

...Jianmin Fang; Peter Rohloff; Kildare Miranda; Roberto Docampo Inorganic poly P (polyphosphate) is an abundant component of acidocalcisomes of Trypanosoma brucei . In the present study we report the presence of a protein homologous with the yeast Vtc1p (vacuolar transporter chaperone 1) in T. brucei...

...Tanja Schlecker; Marcelo A. Comini; Johannes Melchers; Thomas Ruppert; R. Luise Krauth-Siegel Trypanosoma brucei , the causative agent of African sleeping sickness, encodes three nearly identical genes for cysteine-homologues of the selenocysteine-containing glutathione peroxidases. The enzymes...

[email protected] ). 5 2 2007 26 3 2007 3 4 2007 3 4 2007 © The Authors Journal compilation © 2007 Biochemical Society 2007 interfacial activation lipid metabolism lysophosphatidylcholine phospholipase Trypanosoma brucei All eukaryotic organisms are thought...

... trypanosomes possess two isoforms that are localized in the cytosol and in the mitochondrion of the parasites respectively. Here we report on the biological significance of the cTXN (cytosolic TXN) of Trypanosoma brucei for hydroperoxide detoxification. Depending on the growth phase, the concentration...

... is essential for viability in a number of human pathogens, including the fungi Candida albicans and Cryptococcus neoformans , and the parasitic protozoa Leishmania major and Trypanosoma brucei . We have purified the Leishmania and T. brucei NMTs as active recombinant proteins and carried out kinetic analyses...