...Brian S. Mantilla; Lisvane Paes-Vieira; Felipe de Almeida Dias; Simone G. Calderano; Maria Carolina Elias; Daniela Cosentino-Gomes; Pedro L. Oliveira; José Roberto Meyer-Fernandes; Ariel M. Silber The pathogenic protist Trypanosoma cruzi uses kissing bugs as invertebrate hosts that vectorize...

...Letícia Marchese; Karel Olavarria; Brian Suarez Mantilla; Carla Cristi Avila; Rodolpho Ornitiz Oliveira Souza; Flávia Silva Damasceno; Maria Carolina Elias; Ariel Mariano Silber In Trypanosoma cruzi , the etiological agent of Chagas disease, the amino acid proline participates in processes related...

... This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) . human Leishmania phosphofructokinase reverse reaction Trypanosoma cruzi Trypanosoma brucei...

...Damián Estrada; Gabriela Specker; Alejandra Martínez; Patricia Pereira Dias; Barbara Hissa; Luciana O. Andrade; Rafael Radi; Lucía Piacenza Chagas disease (CD), caused by the protozoa Trypanosoma cruzi , is a chronic illness in which parasites persist in the host-infected tissues for years. T...

... pharmacologically validated as a drug target in the African trypanosome, Trypanosoma brucei . In the present study, we evaluate NMT as a potential drug target in Trypanosoma cruzi , the causative agent of Chagas’ disease, using chemical and genetic approaches. Replacement of both allelic copies of TcNMT ( T. cruzi...

...Radika Soysa; Hanka Venselaar; Jacqueline Poston; Buddy Ullman; Marie-Pierre Hasne The TcPOT1.1 gene from Trypanosoma cruzi encodes a high affinity putrescine-cadaverine transporter belonging to the APC (amino acid/polyamine/organocation) transporter superfamily. No experimental three-dimensional...

..., this protein is modified by phosphorylation with unknown functions. In the present study we show that a phosphorylated state of eIF5A predominates in exponentially growing Trypanosoma cruzi cells, and extensive dephosphorylation occurs in cells in stationary phase. Phosphorylation occurs mainly at Ser 2...

...Gaspar E. Cánepa; Maria Sol Degese; Alexandre Budu; Celia R. S. Garcia; Carlos A. Buscaglia TSSA (trypomastigote small surface antigen) is a polymorphic mucin-like molecule displayed on the surface of Trypanosoma cruzi trypomastigote forms. To evaluate its functional properties, we undertook...

...Ivana Urban; Lucía Boiani Santurio; Agustina Chidichimo; Hai Yu; Xi Chen; Juan Mucci; Fernán Agüero; Carlos A. Buscaglia The surface of the protozoan Trypanosoma cruzi is covered by a dense coat of mucin-type glycoconjugates, which make a pivotal contribution to parasite protection and host immune...

... supply of nucleotide-sugars (NDP-sugars), yet little is known about how these NDP-sugars are made and supplied. In the present paper, we report a functional gene from Trypanosoma cruzi that encodes a nucleotidyltransferase, which is capable of transforming different types of sugar 1-phosphates and NTP...

...Sara R. Heras; M. Carmen Thomas; Francisco Macias; Manuel E. Patarroyo; Carlos Alonso; Manuel C. López It has been reported previously that the C2-L1Tc protein located in the Trypanosoma cruzi LINE (long interspersed nuclear element) L1Tc 3′ terminal end has NAC (nucleic acid chaperone) activity...

...Florencia Irigoín; Natalia M. Inada; Mariana P. Fernandes; Lucía Piacenza; Fernanda R. Gadelha; Anibal E. Vercesi; Rafael Radi The epimastigote stage of Trypanosoma cruzi undergoes PCD (programmed cell death) when exposed to FHS (fresh human serum). Although it has been known for over 30 years...

...Lucía Piacenza; Gonzalo Peluffo; María Noel Alvarez; John M. Kelly; Shane R. Wilkinson; Rafael Radi There is increasing evidence that Trypanosoma cruzi antioxidant enzymes play a key immune evasion role by protecting the parasite against macrophage-derived reactive oxygen and nitrogen species...

...Lucía Piacenza; Florencia Irigoín; María Noel Alvarez; Gonzalo Peluffo; Martin C. Taylor; John M. Kelly; Shane R. Wilkinson; Rafael Radi Trypanosoma cruzi undergo PCD (programmed cell death) under appropriate stimuli, the mechanisms of which remain to be established. In the present study, we show...

... eukaryotic genomes, with the remarkable exception of those of trypanosomatids. The genome of the CL Brener clone of Trypanosoma cruzi , the causative agent of Chagas' disease, encodes two such MCPs, with 64% identity between them: Tc MCP-1 and Tc MCP-2. Both genes, which are present in a single copy per...

...Ana L. Stern; Emmanuel Burgos; Laurent Salmon; Juan J. Cazzulo Trypanosoma cruzi , the human parasite that causes Chagas disease, contains a functional pentose phosphate pathway, probably essential for protection against oxidative stress and also for R5P (ribose 5-phosphate) production...

...Rocío Díaz-Benjumea; Sunil Laxman; Thomas R. Hinds; Joseph A. Beavo; Ana Rascón Trypanosoma cruzi , the causative agent of Chagas disease, encodes a number of different cAMP-specific PDE (phosphodiesterase) families. Here we report the identification and characterization of TcrPDEB1 and its...

...Mauricio Vieira; Peter Rohloff; Shuhong Luo; Narcisa L. Cunha-E-Silva; Wanderley De Souza; Roberto Docampo Previous studies in Trypanosoma cruzi , the etiologic agent of Chagas disease, have resulted in the cloning and sequencing of a pair of tandemly linked genes ( TcHA1 and TcHA2 ) that encode P...

... of Trypanosoma cruzi ) is involved in the process of cell invasion, since specific inhibitors block parasite entry into non-phagocytic mammalian host cells. In contrast with other POPs, POP Tc80 is capable of hydrolysing large substrates, such as fibronectin and native collagen. In this study, we present...

... other lower eukaryotes, the protozoan parasite Trypanosoma cruzi is capable of synthesizing free or protein-linked glycoinositolphospholipids containing IPC. As a first step towards understanding the importance and mechanism of IPC synthesis in T. cruzi , we investigated the effects of rustmicin...

...Nisha GARG; Arpad GERSTNER; Vandanajay BHATIA; James DeFORD; John PAPACONSTANTINOU Cardiac hypertrophy and remodelling in chagasic disease might be associated with mitochondrial dysfunction. In the present study, we characterized the cardiac metabolic responses to Trypanosoma cruzi infection...

... localization of TcPDE1, the first cAMP-specific phosphodiesterase to be identified from Trypanosoma cruzi . TcPDE1 is part of a small gene family and encodes a 929-amino-acid protein that can complement a heat-shock-sensitive yeast mutant deficient in phospho-diesterase genes. Recombinant TcPDE1 strongly...

... trypanosome Trypanosoma cruzi . We have isolated a single-copy gene from T. cruzi with the potential to encode an 18kDa enzyme, the sequence of which has highest similarity with glutathione peroxidases from plants. A recombinant form of the protein was purified following expression in Escherichia coli...