1-50 of 60
Keywords: X-ray crystallography
Close
Follow your search
Access your saved searches in your account

Would you like to receive an alert when new items match your search?
Close Modal
Sort by
Articles
Biochem J (2021) BCJ20210117.
Published: 12 April 2021
... Creative Commons licence. phospholipase D substrate recognition X-ray crystallography protein structure catalytic mechanism 1 Structures of an engineered phospholipase D with specificity for secondary alcohol transphosphatidylation: Insights into plasticity of substrate binding and activation...
Articles
Biochem J (2021) 478 (5): 1117–1136.
Published: 10 March 2021
... different stages of the life cycle of the parasite. To gain further insight into its biological activity, we solved the structure of N-terminal histone chaperone domain of PfAsf1 (1–159 amino acids) by X-ray crystallography to a resolution of 2.4 Å. The structure is composed of two beta-sheet to form a beta...
Includes: Supplementary data
Articles
Biochem J (2020) 477 (24): 4769–4783.
Published: 24 December 2020
... of the Biochemical Society 2020 appl1 appl2 crystallography curvature endocytosis heterodimer APPL1 APPL2 curvature endocytosis heterodimerization structure x-ray crystallography In response to extracellular stimuli, signaling transduction usually elicits endocytosis...
Includes: Supplementary data
Articles
Biochem J (2020) 477 (21): 4313–4326.
Published: 13 November 2020
... 22 10 2020 23 10 2020 © 2020 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2020 E-cadherin leptospirosis leucine-rich repeat NGAL X-ray crystallography The interactions between rLRR20 and ECMs or cell surface receptors were...
Articles
Biochem J (2020) 477 (19): 3833–3838.
Published: 12 October 2020
... Biochemical Society 2020 Correspondence: Liansheng Cheng ( charlson@ustc.edu.cn ) or Zhongliang Zhu ( zlzhu63@ustc.edu.cn ) glutamine methylation HEMK2 SAM/SAH TRMT112 x-ray crystallography Crystals were grown using the sitting drop vapor diffusion method at 18°C. For...
Includes: Supplementary data
Articles
Biochem J (2019) 476 (18): 2521–2543.
Published: 14 September 2019
... Creative Commons Attribution License 4.0 (CC BY) . DEAH-box helicase DHX8 RNA binding splicing X-ray crystallography The splicing of pre-mRNA to remove non-coding introns is a crucial process in gene expression in all eukaryotes [ 1 ]. It is estimated that ∼95% of human genes are...
Includes: Supplementary data
Articles
Biochem J (2019) 476 (6): 975–989.
Published: 22 March 2019
... mass spectrometry X-ray crystallography † These authors contributed equally to this work. 3 12 2018 26 2 2019 4 3 2019 5 3 2019 © 2019 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2019 Table 1 X-ray diffraction...
Includes: Supplementary data
Articles
Biochem J (2018) 475 (14): 2257–2269.
Published: 25 July 2018
... phosphatidylinositol 4-phosphate 5-kinase X-ray crystallography The previously solved PIP5K/AMP-PNP complex structure has implied multiple roles of the aspartate residue mutated in LCCS3 [ 12 ]: (1) it binds ATP through a hydrogen bond with the ribose ring of ATP; (2) it forms a hydrogen bond with S301 and...
Includes: Supplementary data
Articles
Biochem J (2017) 474 (4): 539–556.
Published: 03 February 2017
Includes: Supplementary data
Articles
Biochem J (2016) 473 (14): 2023–2032.
Published: 12 July 2016
... inhibitor metalloenzyme X-ray crystallography The carbonic anhydrases (CAs, EC 4.2.1.1) belong to a superfamily of metalloenzymes which use as substrates some unusually chemically simple molecules/ions, as they catalyse the interconversion between CO 2 and bicarbonate [ 1 – 13 ]. Although this...
Articles
Biochem J (2016) 473 (11): 1593–1604.
Published: 27 May 2016
... biology transforming growth factor-β (TGF-β) X-ray crystallography Gremlin-1 is an extracellular antagonist of the bone morphogenetic proteins (BMPs) and functions by directly neutralizing its ligands and inhibiting BMP signalling [ 1 – 4 ]. BMPs, together with their antagonists, are responsible...
Includes: Supplementary data
Articles
Biochem J (2016) 473 (1): 55–66.
Published: 09 December 2015
... structures of the wild-type and various mutants of SAV1875 with different cysteine oxidation states were elucidated. SAV1875 functions as a chaperone and the redox state of Cys 105 may play an important role. chaperone cysteine DJ-1 superfamily oxidation SAV1875 Staphylococcus aureus X-ray...
Includes: Supplementary data
Articles
Biochem J (2015) 468 (2): 245–257.
Published: 22 May 2015
... glycogen, with greater affinity. AMP-activated protein kinase (AMPK) carbohydrate-binding module (CBM) glycogen isothermal titration calorimetry nuclear magnetic resonance spectroscopy X-ray crystallography AMP-activated protein kinase (AMPK) is a key enzyme involved in energy...
Includes: Supplementary data
Articles
Biochem J (2015) 468 (1): 159–166.
Published: 05 May 2015
... C2 domain epidermal growth factor (EGF)-like repeat Notch X-ray crystallography Sedimentation velocity and sedimentation equilibrium experiments were performed using a XL-I analytical ultracentrifuge (Beckman Coulter) equipped with UV/Vis scanning optics. Buffer reference (TBS, 5 mM CaCl 2...
Includes: Supplementary data
Articles
Biochem J (2014) 464 (3): 387–399.
Published: 05 December 2014
... specific activity values with L -amino acids were ~10–50-fold lower than those reported in the original paper [ 4 ], thus lending support to the assumption of aminoacetone as the preferred substrate. aminoacetone 2   5-dimethylpyrazine enzyme structure flavoprotein X-ray crystallography...
Includes: Supplementary data
Articles
Biochem J (2014) 463 (3): 405–412.
Published: 10 October 2014
... determined. This trimeric core binds both E1 and E3 component enzymes to form for the first time a catalytically active mini-complex. archaea macromolecular assembly multienzyme complex 2-oxoacid dehydrogenase thermophile X-ray crystallography The co-ordinates reported for truncated C...
Articles
Biochem J (2014) 462 (3): 581–589.
Published: 22 August 2014
... metal site kynurenine formamidase tryptophan catabolism X-ray crystallography zinc enzyme Tryptophan catabolism via the kynurenine pathway supports the biosynthesis of NAD + , important neuroactive intermediates in eukaryotes, then anthranilate, quinolate and antibiotics in prokaryotes [ 1...
Articles
Biochem J (2014) 462 (3): 415–424.
Published: 22 August 2014
... mechanism MD simulation Salmonella typhi X-ray crystallography A solution of sodium borohydride (2 μl; 0.5 M) in aqueous sodium hydroxide (40 mM) was added to a 1 ml incubation of compound 3 (0.5 mM) and the enzyme (1 unit) in PPB (50 mM; pH 7.2) at 25°C. The DHQ1 activity was assayed before...
Articles
Biochem J (2014) 461 (2): 335–345.
Published: 26 June 2014
...® accession number EF159153), two primers were designed as following: P1, 5′-TCCTCGGTTGGAGCAGCAG-3′ and P2, 5′-GTGGATTCAGCCCAGACCG-3′. In the present study, using a combination of structural (X-ray crystallography), biochemical and biophysical techniques, we present the first structural insights for...
Articles
Biochem J (2014) 457 (3): 425–434.
Published: 10 January 2014
...-oxoadipate; carboligation, EC 2.2.1.5) or the lipoyl domain from E2 for the dehydrogenase reaction (E1o canonical pathway; reductive acylation, EC 1.2.4.2) [ 11 ]. bacterial metabolism decarboxylase dehydrogenase enzyme catalysis Mycobacterium protein dynamics X-ray crystallography...
Includes: Supplementary data
Articles
Biochem J (2013) 454 (3): 491–499.
Published: 29 August 2013
... tornroth@chem.gu.se ). The structural co-ordinates of the SoPIP2;1–mercury complex will appear in the PDB under accession code 4JC6. 13 3 2013 7 6 2013 3 7 2013 3 7 2013 aquaporin mercury plasma membrane intrinsic protein proteoliposome assay water transport X-ray...
Includes: Supplementary data
Articles
Biochem J (2013) 454 (3): 459–466.
Published: 29 August 2013
.... 21 12 2012 6 6 2013 28 6 2013 28 6 2013 © The Authors Journal compilation © 2013 Biochemical Society 2013 microtubule-associated protein light chain 3 beta (LC3) NMR spectroscopy optineurin protein–protein interaction selective autophagy X-ray crystallography...
Includes: Supplementary data
Articles
Biochem J (2013) 451 (2): e1–e3.
Published: 28 March 2013
... therapeutics. 1 email esundberg@ihv.umaryland.edu 26 2 2013 1 3 2013 © The Authors Journal compilation © 2013 Biochemical Society 2013 antibody asthma interleukin 4 (IL-4) interleukin 13 (IL-13) X-ray crystallography Asthma is an inflammatory respiratory disorder...
Articles
Biochem J (2013) 450 (3): 469–476.
Published: 28 February 2013
... Langer G. Cohen S. X. Lamzin V. S. Perrakis A. Automated macromolecular model building for X-ray crystallography using ARP/wARP version 7 Nat. Protoc. 2008 3 1171 1179 29 Murshudov G. N. Vagin A. A. Dodson E. J. Refinement of macromolecular structures by the...
Includes: Supplementary data
Articles
Biochem J (2013) 449 (2): 469–477.
Published: 14 December 2012
.../licenses/by-nc/2.5/ ) which permits unrestricted non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited. early secretory antigenic target of 6 kDa system 1 (ESX-1) Gram-positive bacterium protein kinase protein secretion pseudokinase X-ray...
Includes: Supplementary data
Articles
Biochem J (2013) 449 (2): 415–425.
Published: 14 December 2012
... lipoate protein ligase lipoyl domain NMR spectroscopy protein–protein interaction X-ray crystallography Aerobic metabolism of 2-oxoacids and C1 metabolism are dependent on LA (lipoic acid) in a highly conserved manner [ 1 ]. LA is an essential co-factor of the OADHCs (2-oxoacid dehydrogenase...
Articles
Biochem J (2012) 448 (3): 417–423.
Published: 21 November 2012
..., distribution and reproduction in any medium, provided the original work is properly cited. cancer epidermal growth factor receptor (EGFR) erlotinib gefitinib inhibitor molecular dynamics receptor tyrosine kinase (RTK) X-ray crystallography Activating mutations in the EGFR (epidermal growth...
Includes: Supplementary data
Articles
Biochem J (2012) 448 (2): 213–220.
Published: 07 November 2012
... orphan receptor 2 (Ror2) tropomyosin receptor kinase A (TrkA) X-ray crystallography Recent clinical success with targeted inhibitors of the kinases EGFR (epidermal growth factor receptor), B-Raf and ALK (anaplastic lymphoma kinase) underscore both the promise and the challenges of ‘personalized...
Articles
Biochem J (2012) 448 (1): 55–65.
Published: 18 October 2012
...- and two MPN-containing proteins in the 19S proteasome lid can be identified in the CSN complex [ 32 ]. Rpn12 consists of an N-terminal PCI domain followed by a C-terminal tail of unknown structure and corresponds to CSN8 and eIF3k. PCI domain proteasome ubiquitin X-ray crystallography...
Includes: Supplementary data
Articles
Biochem J (2012) 447 (3): 335–351.
Published: 05 October 2012
... 7 2012 23 7 2012 © The Authors Journal compilation © 2012 Biochemical Society 2012 calcium-binding domain structure enzyme activation inhibitor complex proteinase proteolysis X-ray crystallography The present review will focus on insights about calpains and their...
Includes: Multimedia, Supplementary data
Articles
Biochem J (2012) 446 (1): 149–157.
Published: 27 July 2012
.... chitinase chitinase-like proteins glycan glycan array glycobiology protein structure lectin X-ray crystallography The co-ordinates and structure factors have been deposited in the PDB under accession code 4AY1. 1 These authors contributed equally to this work. 2 To whom...
Articles
Biochem J (2011) 440 (1): 51–61.
Published: 27 October 2011
... 28 7 2011 © The Authors Journal compilation © 2011 Biochemical Society 2011 bacteriochlorophyll light-harvesting complex puc gene purple photosynthetic bacterium Rhodopseudomonas palustris X-ray crystallography In some species of purple bacteria, such as Rps...
Includes: Supplementary data
Articles
Biochem J (2011) 437 (3): 413–422.
Published: 13 July 2011
... isomerase activity and are inhibited by drugs including rapamycin and tacrolimus. We determined the structure of the Mip homologue [BpML1 ( Burkholderia pseudomallei Mip-like protein 1)] from the human pathogen and biowarfare threat B. pseudomallei by NMR and X-ray crystallography. The crystal structure...
Includes: Supplementary data
Articles
Biochem J (2010) 432 (2): 237–247.
Published: 12 November 2010
... 24 8 2010 1 9 2010 1 9 2010 © The Authors Journal compilation © 2010 Biochemical Society 2010 Bacillus subtilis inositol dehydrogenase myo inositol dehydrogenase site-directed mutagenesis structural classification X-ray crystallography myo -Inositol is...
Includes: Supplementary data
Articles
Biochem J (2010) 428 (1): 67–74.
Published: 28 April 2010
... comprises two homologous domains, the differing substrate preferences of which present a new avenue for domain-selective inhibitor design. We have co-crystallized lisW-S, a C-domain-selective derivative of the drug lisinopril, with human testis ACE and determined a structure using X-ray crystallography to a...
Includes: Supplementary data
Articles
Biochem J (2010) 425 (3): 513–522.
Published: 15 January 2010
... 2009 3 11 2009 3 11 2009 © The Authors Journal compilation © 2010 Biochemical Society 2010 Chagas disease enzyme mechanism NMR spectroscopy peroxidase X-ray crystallography Across the tropics over 20 million people are infected by the protozoan parasites Trypanosoma...
Includes: Supplementary data
Articles
Biochem J (2009) 424 (3): 347–356.
Published: 10 December 2009
... Bacillus subtilis copper cluster copper-mediated protein–protein interaction copper trafficking cysteine thiol X-ray crystallography Copper is involved in a wide range of biochemical processes, including aerobic and anaerobic respiration, oxidative stress response, iron and oxygen transport...
Includes: Supplementary data
Articles
Biochem J (2009) 421 (1): 97–106.
Published: 12 June 2009
... reactivation tabun analogues X-ray crystallography Human plasma BChE (butyrylcholinesterase; EC 3.1.1.8) is a toxicologically relevant enzyme because it hydrolyses or scavenges a wide range of toxic esters, including heroin, cocaine, carbamate pesticides, organophosphorus pesticides and nerve agents...
Articles
Biochem J (2009) 421 (1): 87–96.
Published: 12 June 2009
... stability protein structure X-ray crystallography Glycosylation is an important cellular modification, and it is related to many human diseases and developmental defects [ 1 ]. It introduces diversity into a biological system because of its inherent structural heterogeneity, and thus plays...
Includes: Supplementary data
Articles
Biochem J (2008) 415 (2): 217–223.
Published: 25 September 2008
... drastic effects on steady-state kinetics, suggesting that the differences could be exploitable with small-molecule inhibitors. Aspergillus fumigatus inhibitor design kinetics mutagenesis protein structure UDP-GlcNAc biosynthesis X-ray crystallography Aspergillus fumigatus is a...
Articles
Biochem J (2008) 414 (1): 81–91.
Published: 29 July 2008
...) thioredoxin-like protein X-ray crystallography Thiol–disulfide oxidoreductases (TDORs) occur in most cell types, where they function in the control of the redox state of cysteine-residue side chains. Many TDORs are structurally highly related and contain the active-site motif CXXC (amino acid one...
Includes: Supplementary data
Articles
Biochem J (2008) 411 (2): 241–247.
Published: 27 March 2008
... 12 12 2007 © The Authors Journal compilation © 2008 Biochemical Society 2008 Chlamydomonas reinhardtii chloroplast mutant proximal cysteine ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) specificity factor X-ray crystallography The bifunctional enzyme Rubisco...
Includes: Supplementary data
Articles
Biochem J (2008) 411 (1): 181–190.
Published: 13 March 2008
... synchrotron X-ray crystallography QCs (glutaminyl cyclases; glutaminyl-peptide cyclotransferases, EC 2.3.2.5) catalyse the conversion of protein N-terminal glutamine (or glutamic acid) residue into pGlu (pyroglutamate), a reaction that is important during the maturation of many bioactive peptides...
Includes: Supplementary data
Articles
Biochem J (2007) 408 (1): 29–38.
Published: 29 October 2007
... trichosurin was solved by X-ray crystallography in two different crystal forms to 1.9 Å (1 Å=0.1 nm) and 2.6 Å resolution, from crystals grown at low and high pH values respectively. Trichosurin has the typical lipocalin fold, an eight-stranded anti-parallel β-barrel but dimerizes in an orientation that has...
Includes: Supplementary data
Articles
Biochem J (2006) 399 (3): 427–434.
Published: 13 October 2006
... Society, London 2006 protein phosphatase transforming growth factor-β-activated kinase 1-binding protein 1 (TAB1) transforming growth factor-β-activated kinase 1 (TAK1) pro-inflammatory cytokine X-ray crystallography TAK1 (transforming growth factor-β-activated kinase 1) is activated...
Articles
Biochem J (2006) 399 (2): 199–204.
Published: 27 September 2006
... Society, London 2006 angiogenesis human thymidine phosphorylase product inhibition pyrimidine nucleoside phosphorylase structure X-ray crystallography HTP (human thymidine phosphorylase; EC 2.4.2.4) belongs to the PYNP (pyrimidine nucleoside phosphorylase) family and catalyses the...
Articles
Biochem J (2006) 398 (1): 37–43.
Published: 27 July 2006
.... 16 2 2006 19 4 2006 28 4 2006 28 4 2006 The Biochemical Society, London 2006 carbohydrate-active enzyme cellobiose phosphorylase chitobiose phosphorylase glycoside hydrolase family 94 substrate specificity X-ray crystallography The enzymes involved in oligo...
Articles
Biochem J (2006) 397 (2): 271–278.
Published: 28 June 2006
... binding Ig-like lectin (siglec) x-ray crystallography The siglec (sialic acid binding Ig-like lectin) family is a group of transmembrane receptors with the ability to recognize sialic acids, a family of 9-carbon sugars which often cap the non-reducing ends of glycoconjugates in higher animals...
Articles
Biochem J (2005) 392 (1): 55–63.
Published: 08 November 2005
... 2005 The Biochemical Society, London 2005 catalytic mechanism D -alanine carboxypeptidase deacylation Escherichia coli penicillin-binding protein X-ray crystallography During the final stages of cell-wall synthesis in bacteria, enzymes known as penicillin-binding proteins...
Articles
Biochem J (2005) 389 (2): 365–371.
Published: 05 July 2005
... whom correspondence should be addressed (email hosur@magnum.barc.ernet.in ). 27 10 2004 17 3 2005 24 3 2005 24 3 2005 The Biochemical Society, London 2005 catalysis HIV-1 protease inhibitor reaction intermediate transition state X-ray crystallography...