.... We tested their involvement using site-directed mutagenesis (SDM) of MCT1 to change residues individually or in combination with their MCT4 equivalent and determined inhibitor sensitivity following expression in Xenopus oocytes. Phe 360 and Ser 364 were identified as important for AR-C155858 binding...

..., distribution and reproduction in any medium, provided the original work is properly cited. chimaeric transporter erythrocyte lactate transport monocarboxylate transporter (MCT) Xenopus oocytes There are 14 members of the MCT (monocarboxylate transporter) family (SLC16) encoded by the human...

.... The other cDNA has a 5′-untranslated region sequence that is distinct from that of full-length mENT1. Microinjection of mENT1, mENT1b or mENT2 cRNA into Xenopus oocytes resulted in enhanced uptake of [ 3 H]uridine by the oocytes relative to that seen in water-injected controls. mENT1-mediated, but not mENT2...

...Ana M. PAJOR; Esther S. KAHN; Rama GANGULA The role of cationic amino acids in the Na + /dicarboxylate co-transporter NaDC-1 was investigated by site-directed mutagenesis and subsequent expression of mutant transporters in Xenopus oocytes. Of the ten residues chosen for mutagenesis, eight (Lys-34...

...: Department of Pathology, University of Arizona, Tucson, AZ 85721, U.S.A. 12 7 1999 20 9 1999 The Biochemical Society, London © 1999 1999 citrate mutagenesis succinate Xenopus oocytes Biochem. J. (1999) 344, 205 209 (Printed in Great Britain) 205 Cysteine residues in the Na...