... level of energy regulation and its role in the maturation of cardiac metabolism after birth and cardiac adaptability for different metabolic conditions and energy demands. The role post-translational modifications, namely phosphorylation, acetylation, malonylation, succinylation and glutarylation, play...

... that Hpa2- Ab confers resistance against aminoglycoside antibiotics using Escherichia coli DH5α strains in which Hpa2 gene is expressed. Resistivity for aminoglycoside antibiotics is demonstrated with the help of CLSI-2010 and KB tests. Isothermal titration calorimetry, MALDI and acetylation assays indicate...

... understood, but evidence suggests a potential regulatory role for the sirtuin family of lysine deacetylases. Thus, we hypothesized that the acetylation status of the major 2-HG-generating enzymes [lactate dehydrogenase (LDH), isocitrate dehydrogenase (IDH) and malate dehydrogenase (MDH)] may govern their 2...

...Carla Ritagliati; Gabriela Vanina Villanova; Victoria Lucia Alonso; Aline Araujo Zuma; Pamela Cribb; María Cristina Machado Motta; Esteban Carlos Serra Acetylation is a ubiquitous protein modification present in prokaryotic and eukaryotic cells that participates in the regulation of many cellular...

..., but its cytoplasmic roles are still not elucidated fully. We found that blocking HDAC3 activity using MI192, a compound specific for HDAC3, modulated tubulin acetylation in the human prostate cancer cell line PC3. A brief 1 h treatment of PC3 cells with MI192 significantly increased levels of tubulin...

... glycosylase activity and deacetylates acetylated TDG. TDG acetylation weakens its interaction with SIRT1. Although acetylated TDG has reduced glycosylase activity towards T/G, 5-formylcytosine/G and 5-carboxylcytosine/G, it has a stronger activity towards a 5-fluorouracil/G substrate as compared...

... To whom correspondence should be addressed (email [email protected] ). 24 10 2012 13 12 2012 18 12 2012 © The Authors Journal compilation © 2013 Biochemical Society 2013 acetylation chromatin epigenetics inhibitor of growth (ING) stress tumour suppressor The ING...

... structure, regulation of microtubule dynamics during adipogenesis remains unclear. In the present paper we show that acetylation of α-tubulin is up-regulated during adipogenesis, and adipocyte development is dependent on α-tubulin acetylation, as expression of an acetylation-resistant α-tubulin mutant...

.... Metabolic inflexibility occurred concomitantly with decreased activities of PDH (pyruvate dehydrogenase) and complex II. Hyper-acetylation of protein lysine was also observed. Treatment of control heart mitochondria with acetic anhydride (Ac 2 O), an acetylating agent, preferentially inhibited respiration...

... of Victoria, Victoria, BC, Canada 2 To whom correspondence should be addressed (email [email protected] ). 27 3 2012 27 4 2012 30 4 2012 30 4 2012 © The Authors Journal compilation © 2012 Biochemical Society 2012 acetylation arylamine N...

... (trimethylated Lys 4 of histone H3) and H3K9ac (acetylated Lys 9 of histone H3) at the active Glyt1a promoter do not play a major role in recruiting HMGN proteins. HMGN3a/b binding leads to increased H3K14 (Lys 14 of histone H3) acetylation and stimulates Glyt1a expression, but does not alter the levels...

... myosin function. The fission yeast Schizosaccharomyces pombe possesses a single essential Tm, Cdc8, which can be acetylated on its N-terminal methionine residue to increase its affinity for actin and enhance its ability to regulate myosin function. We have designed and generated a number of novel Cdc8...

... by the post-translational synthesis of hypusine and the hypusine modification is essential for cell proliferation. In the present study, we report selective acetylation of the hypusine and/or deoxyhypusine residue of eIF5A by a key polyamine catabolic enzyme SSAT1 (spermidine/spermine-N 1 -acetyltransferase 1...

... the unspliced form by IRE1 (inositol-requiring enzyme 1) during the UPR. However, the post-translational modulation of XBP1s remains largely unknown. In the present study, we demonstrate that XBP1s is a target of acetylation and deacetylation mediated by p300 and SIRT1 (sirtuin 1) respectively. p300 increases...

...Kyung-Chul Choi; SiYong Park; Beom Jin Lim; Ah-Reum Sung; Yoo-Hyun Lee; Masaki Shiota; Akira Yokomizo; Seiji Naito; Younghwa Na; Ho-Geun Yoon Increasing evidence suggests that AR (androgen receptor) acetylation is critical for prostate cancer cell growth. In the present study, we identified Pro-B3...

... Sp1 at the GT-box. Immunoprecipitation experiments have shown that the acetylation of Sp1 and Sp3 is increased by TSA treatment; however, no effect on DNA binding was observed. Histone acetyltransferases such as p300 and P/CAF [p300/CREB (cAMP-response-element-binding protein)-binding protein...

...Guillermo G. Zampar; María E. Chesta; Agustín Carbajal; Natalí L. Chanaday; Nicolás M. Díaz; César H. Casale; Carlos A. Arce We showed previously that NKA (Na + /K + -ATPase) interacts with acetylated tubulin resulting in inhibition of its catalytic activity. In the present work we determined...

...René Assenberg; Michelle Webb; Edward Connolly; Katherine Stott; Matthew Watson; Josie Hobbs; Jean O. Thomas The structure-specific DNA-binding protein HMGB1 (high-mobility group protein B1) which comprises two tandem HMG boxes (A and B) and an acidic C-terminal tail, is acetylated in vivo at Lys 2...

... not explain CFTR -related gastrointestinal disease patterns and that epigenetic modifiers influence CFTR expression. Our aim was to characterize the native chromatin environment in cultured cells for intestinal CFTR expression by determining the relationship between histone acetylation and occupation of CFTR...

...Sharon Wong; Jason D. Weber The activity of Rb (retinoblastoma protein) is regulated by phosphorylation and acetylation events. Active Rb is hypophosphorylated and acetylated on multiple residues. Inactivation of Rb involves concerted hyper-phosphorylation by cyclin–CDK (cyclin-dependent kinase...

... that the N-terminal domain of MAML1 directly interacts with both p300 and histones, and the p300–MAML1 complex specifically acetylates histone H3 and H4 tails in chromatin. Furthermore, p300 acetylates MAML1 and evolutionarily conserved lysine residues in the MAML1 N-terminus are direct substrates for p300...

...Ahmed H. Hassan; Salma Awad; Zeina Al-Natour; Samah Othman; Farah Mustafa; Tahir A. Rizvi Bromodomains are present in many chromatin-associated proteins such as the SWI/SNF and RSC chromatin remodelling and the SAGA HAT (histone acetyltransferase) complexes, and can bind to acetylated lysine...

[email protected] ). 9 1 2006 7 4 2006 17 5 2006 17 5 2006 The Biochemical Society, London 2006 acetylation cAMP-response-element-binding-protein-binding protein/p300 (CBP/p300) histone acetyltransferase activity (HAT activity) murine double minute 2 (Mdm2) NIH 3T3 cell Ras...

... 1 -acetylated polyamines can be excreted or oxidized by acetylpolyamine oxidase. We have purified a recombinant human acetyltransferase (SSAT2) that shares 45% identity and 61% homology with human SSAT1, but is only distally related to other known members of the GNAT (GCN5-related N...

...Hiroto IZUMI; Ryo OHTA; Gunji NAGATANI; Tomoko ISE; Yoshifumi NAKAYAMA; Minoru NOMOTO; Kimitoshi KOHNO We demonstrated recently that expression of the UDP- N -acetyl-α- d -galactosamine: polypeptide N -acetylgalactosaminyltrans-ferase-3 (GalNAc-T3) gene is restricted to epithelial glands [Nomoto...

...Joseph J. BUGGY; Mindy L. SIDERIS; Polly MAK; Donald D. LORIMER; Brian MCINTOSH; James M. CLARK Histone deacetylases (HDACs) are a growing family of enzymes implicated in transcriptional regulation by affecting the acetylation state of core histones in the nucleus of cells. HDACs are known to have...