...; Abcam), Total PAK1 (1 : 1000; Cell Signaling Technologies), β-actin (1 : 10 000; Sigma–Aldrich), α-tubulin (1 : 20 000; Sigma–Aldrich), GAPDH (1 : 20 000; Abcam). actin cytoskeleton β-catenin insulin secretion p21-activated kinases INS-1E cells were co-transfected with either WT FLAG...

...: Carlos M Farinha ( [email protected] ) actin cytoskeleton cAMP CFTR EPAC1 protein trafficking Cystic Fibrosis (CF) is the most common lethal and life-limiting autosomic recessive disorder among Caucasian population, affecting about 1 : 2500–6000 new borns [ 1 ] and it is caused...

...Fumihiko Nakamura Cell migration, phagocytosis and cytokinesis are mechanically intensive cellular processes that are mediated by the dynamic assembly and contractility of the actin cytoskeleton. GAPs (GTPase-activating proteins) control activities of the Rho family proteins including Cdc42, Rac1...

... 8 2010 7 10 2010 12 10 2010 © The Authors Journal compilation © 2011 Biochemical Society 2011 actin cytoskeleton exocyst complex invasion motility neuronal development small GTPase In the model shown in Figure 3 , additional components are required to interface...

... concentrations where there are no observed effects on Keap1 or cytotoxicity. actin cytoskeleton focal adhesion post-translational protein modification reactive lipid species redox signalling thiol 1 To whom correspondence should be addressed (email [email protected] ). 4 8 2009 3 6...

... ]. actin cytoskeleton cancer inflammatory response knockout-mouse study neurological disorder p21-activated kinase (PAK) The Rho-family GTPases, of which Rho, Rac and Cdc42 are the most studied isoforms, act as molecular switches regulating diverse cellular processes from cell adhesion...

... correspondence should be addressed (email [email protected] ). 1 10 2007 15 1 2008 23 1 2008 23 1 2008 © The Authors Journal compilation © 2008 Biochemical Society 2008 actin cytoskeleton GTP-binding protein Rho GTPase Rho-associated kinase (ROCK) RhoE (Rnd3...

... the prevention of calponin's actin-binding activities. 1 To whom correspondence should be addressed (email [email protected] ). 19 10 2005 2 3 2006 15 3 2006 15 3 2006 The Biochemical Society, London 2006 actin cytoskeleton actin-nucleating activity calponin...

.... We have shown that NF-κB activation in response to the perturbation of actin dynamics required reactive oxygen species, as demonstrated by the effect of antioxidants. Actin cytoskeleton disruption by cytochalasin D induced O 2 − release from human monocytes, through the activation of the NADPH...

...Jocelyn MÉRÉ; Anne CHAHINIAN; Sutherland K. MACIVER; Abdellatif FATTOUM; Nadir BETTACHE; Yves BENYAMIN; Claude ROUSTAN Gelsolin is a calcium-, pH- and lipid-dependent actin filament severing/capping protein whose main function is to regulate the assembly state of the actin cytoskeleton. Gelsolin...

... The Biochemical Society, London 2005 actin cytoskeleton cAMP cell motility prostaglandin E 2 protein kinase A Rac Cell migration plays a critical role in a wide variety of physiological and pathological phenomena, including morphogenic processes during embryogenesis, inflammatory responses...

... in these responses. 1 To whom correspondence should be addressed, at the Ludwig Institute for Cancer Research (email [email protected] ). 15 9 2004 8 10 2004 21 10 2004 21 10 2004 The Biochemical Society, London 2005 actin cytoskeleton intercellular adhesion molecule-1...

... should be addressed (email [email protected] ). 4 3 2004 7 7 2004 7 7 2004 7 7 2004 The Biochemical Society, London 2004 actin cytoskeleton Ca 2+ calpain myristoylated alanine-rich C kinase substrate (MARCKS) myogenesis protein kinase Cα (PKCα...

... excitation wavelengths using the equation as described by Grynkiewicz et al. [ 33 ]. actin cytoskeleton brown adipocyte extracellular ATP P2 receptor store-operated Ca 2+ entry Brown adipose tissue is known to be specialized for non-shivering thermogenesis in mammalian species...

... cytoskeleton remodelling. 1 To whom correspondence should be addressed (e-mail [email protected] ). 2 2 2004 16 3 2004 19 3 2004 19 3 2004 The Biochemical Society, London ©2004 2004 actin cytoskeleton depolymerization cortactin Fer kinase latrunculin B...

... at the apical membrane. 1 To whom correspondence should be addressed (e-mail [email protected] ). 15 10 2003 13 2 2004 16 2 2004 16 2 2004 The Biochemical Society, London ©2004 2004 actin cytoskeleton apical domain cortactin binding protein 1 (CortBP1) lipid raft...

... organization of the actin cytoskeleton. 1 e-mail [email protected] 5 4 2004 14 4 2004 25 5 2004 The Biochemical Society, London ©2004 2004 actin cytoskeleton actin polymerization Arp2/3 complex Fyn/Fer kinases tyrosine phosphorylation Biochem. J. (2004) 380, e7 e8...

... modifications are necessary for mem- brane association of reggie-1. Overexpression of reggie-1 resulted in the induction of numerous filopodia-like protrusions in various cell lines, suggesting a role for reggie-1 as a signalling protein in actin-dependent processes. Key words: actin cytoskeleton, filopodia...

... is mediated by an interaction of the endoplasmic reticulum (ER) with the plasma membrane (PM) and is modulated by the actin cytoskeleton. To explore this possibility further we have now investigated the role of the actin cytoskeleton in the activation and maintenance of SMCE in pancreatic acinar cells, a more...

... independent of the CaM substrate interaction. We also propose that TIMP-2 acts as a negative regulator of MT1-MMP-dependent activities promoted by the action of CaM inhibitors in U-87 glioblastoma cells. Key words: actin cytoskeleton, cell migration, glioma, MT1- MMP, TIMP-2. membrane domain does not seem...

... is supported by electron microscopy, which demonstrates that merlin binds periodically along cyto- skeletal actin filaments. Comparison of these findings with those reported for ERM proteins reveal a distinct role for merlin in actin filament dynamics. Key words: actin cytoskeleton, ezrin}radixin}moesin family...

... that, as with secretion, the actin filaments at the cell periphery act as a negative clamp which prevents constitutive coupling. In addition, the actin cytoskeleton plays a positive role, since disruption of the actin network inhibited the coupling when the Ca 2+ stores were depleted. These results provide strong...

... to the disruption of a key structural element. 1 To whom correspondence should be addressed (e-mail [email protected] ). 18 2 2000 22 5 2000 7 6 2000 The Biochemical Society, London © 2000 2000 actin cytoskeleton glycine-rich repeat muscle myogenesis Biochem. J...

... Institute, Graduate School of Science, Tohoku University, Aoba-ku, Sendai 980-8578, Japan (e-mail [email protected] ). 26 4 1999 17 6 1999 16 7 1999 The Biochemical Society, London © 1999 1999 actin cytoskeleton LIM domain LIM-kinase protein kinase PDZ domain...

...Roland B. GREGORY; Robert A. WILCOX; Leise A. BERVEN; Nicole C. R. VAN STRATEN; Gijs A. VAN DER MAREL; Jacques H. VAN BOOM; Gregory J. BARRITT The roles of a subregion of the endoplasmic reticulum (ER) and the cortical actin cytoskeleton in the mechanisms by which Ins(1,4,5) P 3 induces...