... and describe the status of development of therapeutic strategies to target tumor immune evasion mechanisms with focus on how the tumor microenvironment interacts with T cells. Considering the elevated adenosine concentration in the TME, targeting the immunosuppressive effects of the adenosine pathway has...

..., adenosine and 2′-deoxyadenosine, and no capacity to transport hypoxanthine or AMP. [ 3 H]Adenine transport was sensitive to protons and the ENT inhibitor dipyridamole. 2′-[ 14 C]Deoxyadenosine uptake was inhibited by plant hormone cytokinin molecules with micromolar apparent affinity. Competition studies...

...Sonia Fernández-Veledo; Isabel Huber-Ruano; Ivette Aymerich; Sylvie Duflot; F. Javier Casado; Marçal Pastor-Anglada CNT2 (concentrative nucleoside cotransporter) is a plasma membrane high-affinity Na + -coupled adenosine transporter, also localized in intracellular structures. This transporter...

...Hiroshi Kubo; Kaoru Hazeki; Shunsuke Takasuga; Osamu Hazeki We prepared CHO (Chinese hamster ovary) cells expressing both IR (insulin receptor) and A 1 R (A 1 adenosine receptor). Treatment of the cells with insulin or PIA [ N 6 -(2-phenylisopropyl)adenosine], a specific A 1 R agonist increased Akt...

... sites (∼1.5-fold) for the es -specific probe [ 3 H]NBMPR ([ 3 H]nitrobenzylthioinosine), and increased (∼1.8-fold) the V max for 2-chloro[ 3 H]adenosine of the NBMPR-sensitive ( es ) nucleoside transporter. There was a concomitant decrease in the V max of the NBMPR-resistant ( ei -mediated) uptake of 2...

... by intestinal epithelial cells have not been investigated. In the present study we investigated the role of adenosine, whose levels are up-regulated during inflammation, in modulating FN synthesis, the polarity of FN secretion and the downstream effects of the secreted FN. Polarized monolayers of T84 cells were...

...Rosa J. TORRES; Isabel DeANTONIO; Carmen PRIOR; Juan G. PUIG We postulated that adenosine function could be related to some of the neurological features of Lesch–Nyhan syndrome and therefore characterized adenosine transport in PBLs (peripheral blood lymphocytes) obtained from Lesch–Nyhan patients...

... partially removed. After 7M urea treatment, both α i1 and βγ subunits are required to restore high-affinity agonist binding and receptor-catalysed guanosine 5′-[γ-thio]triphosphate binding. We demonstrate the generality of this approach for four G i -coupled receptors (α 2A -adrenergic, adenosine A1, 5...

... of adipocytes with R -phenylisopropyladenosine, an adenosine A 1 receptor agonist, increased the transport of 3- O -methylglucose and trypsin-accessible GLUT4, in adipocytes from both pair-fed and ethanol-fed rats. These results demonstrate that whereas the insulin-mediated signalling and translocation of GLUT4...

... adenosine with a considerably higher apparent affinity ( K m 0.32±0.05 mM) than the pyrimidine nucleoside uridine ( K m 3.5±1.1 mM). It also efficiently transports nucleobases such as adenine ( K m 0.32±0.10 mM) and hypoxanthine ( K m 0.41±0.1 mM), and anti-viral 3ʹ-deoxynucleoside analogues. Moreover...

...Cynthia M. FERRARA; Samuel W. CUSHMAN Agents that activate the G-protein G i (e.g. adenosine) increase, and agents that activate G s [e.g. isoprenaline (isoproterenol)] decrease, steady-state insulin-stimulated glucose transport activity and cell-surface GLUT4 in isolated rat adipose cells without...

...-dependent enzymes by polyglutamated derivatives, or indirectly to adenosine release and/or apoptosis and clonal deletion of activated peripheral blood lymphocytes in S-phase. In this study of phytohaemagglutinin-stimulated primary human T-lymphocytes we show that MTX (20 nM to 20 μ M) was cytostatic...