... to the dynamic interplay between Ca 2+ and cAMP signalling in regulating the activity of Ca 2+ -sensitive adenylate cyclase isoforms (AC1, AC5, AC6 and AC8). Two proteins have been identified as key components of SOCE: STIM1 (stromal interaction molecule 1), which senses ER Ca 2+ store content and translocates...

... activates or inhibits adenylate cyclases in order to induce proper physiological responses. A key event in this signalling system is the direct and dynamic interaction of Gα i1 subunits with adenylate cyclases. We established a FRET-based assay between G-protein subunits and AC5 (type 5 adenylate cyclase...

...Jürgen U. Linder Class I adenylate cyclases are found in γ- and δ-proteobacteria. They play central roles in processes such as catabolite repression in Escherichia coli or development of full virulence in pathogens such as Yersinia enterocolitica and Vibrio vulnificus . The catalytic domain...

... death in macrophages. EF is an adenylate cyclase that is activated by calmodulin through a non-canonical mechanism. EF causes sustained and potent activation of host cAMP-dependent signalling pathways, which disables phagocytes. Here I review recent progress in elucidating the mechanisms by which LF...

...Elisa Alvarez-Curto; Karin E. Weening; Pauline Schaap Intracellular and secreted cAMPs play crucial roles in controlling cell movement and gene regulation throughout development of the social amoeba Dictyostelium discoideum . cAMP is produced by three structurally distinct ACs (adenylate cyclases...

...Avinash R. SHENOY; Nandini P. SREENATH; Mohana MAHALINGAM; Sandhya S. VISWESWARIAH Analysis of the genome sequence of Mycobacterium tuberculosis H37Rv has identified 16 genes that are similar to the mammalian adenylate and guanylate cyclases. Rv1647 was predicted to be an active adenylate cyclase...

...Zhiyong GAO; Robert A. YOUNG; Matteo M. TRUCCO; Scott R. GREENE; Erik L. HEWLETT; Franz M. MATSCHINSKY; Bryan A. WOLF Activation of protein kinase A (cAMP-dependent protein kinase; PKA) triggers insulin secretion in the β-cell. Adenylate cyclase toxin (ACT), a bacterial exotoxin with adenylate...

...Kaori SATO-KUSUBATA; Yukiko YAJIMA; Seiichi KAWASHIMA Treatment of rat pituitary GH 4 C 1 cell membranes with calpain, a calcium-activated cysteine protease, increased adenylate cyclase activity, and this activity was inhibited by a calpain inhibitor, leupeptin. Calpain treatment potentiated...

... factor for negative regulation by treatment with CTX and PMA. In contrast with the cAMP response, the inhibition of membrane adenylate cyclase activity and the agonist competition curve were not influenced by treatment with CTX or PMA, suggesting that a cytosolic factor was involved in this negative...

... stimulated adenylate cyclase activity in clones expressing both IP prostanoid receptor and the IP prostanoid receptor-G s α fusion protein, and both constructs were shown to interact with and activate endogenously expressed G s α. Addition of iloprost to membranes of cells expressing the isolated receptor...

... is coupled via both PTX- sensitive and -insensitive G-proteins to multiple effector pathways. Key words: adenylate cyclase, G-protein-coupled receptor, lysophospholipid, mitogen-activated protein kinase, phospholipase C. and SPC activate multiple intracellular signalling molecules, including phospholipase C...