Skip Nav Destination
Close Modal
Update search
Filter
- Title
- Author
- Author Affiliations
- Full Text
- Abstract
- Keyword
- DOI
- ISSN
- EISSN
- Issue
- Volume
- References
Filter
- Title
- Author
- Author Affiliations
- Full Text
- Abstract
- Keyword
- DOI
- ISSN
- EISSN
- Issue
- Volume
- References
Filter
- Title
- Author
- Author Affiliations
- Full Text
- Abstract
- Keyword
- DOI
- ISSN
- EISSN
- Issue
- Volume
- References
Filter
- Title
- Author
- Author Affiliations
- Full Text
- Abstract
- Keyword
- DOI
- ISSN
- EISSN
- Issue
- Volume
- References
Filter
- Title
- Author
- Author Affiliations
- Full Text
- Abstract
- Keyword
- DOI
- ISSN
- EISSN
- Issue
- Volume
- References
Filter
- Title
- Author
- Author Affiliations
- Full Text
- Abstract
- Keyword
- DOI
- ISSN
- EISSN
- Issue
- Volume
- References
NARROW
Format
Subjects
Article Type
Date
Availability
1-34 of 34
Keywords: adipocyte
Close
Follow your search
Access your saved searches in your account
Would you like to receive an alert when new items match your search?
Sort by
Articles
In Collection
Adipose biology
Journal:
Biochemical Journal
Biochem J (2020) 477 (5): 985–1008.
Published: 13 March 2020
...Alexander Yang; Emilio P. Mottillo Fatty acids (FAs) are stored safely in the form of triacylglycerol (TAG) in lipid droplet (LD) organelles by professional storage cells called adipocytes. These lipids are mobilized during adipocyte lipolysis, the fundamental process of hydrolyzing TAG to FAs...
Articles
Journal:
Biochemical Journal
Biochem J (2016) 473 (19): 3177–3188.
Published: 27 September 2016
... proteins. A role of IQGAP1 in adipocytes has not been reported. We therefore investigated the cellular IQGAP1 interactome in primary human adipocytes. Immunoprecipitation and quantitative mass spectrometry identified caveolae and caveolae-associated proteins as the major IQGAP1 interactors alongside...
Articles
Paul Duffield Brewer, Estifanos N. Habtemichael, Irina Romenskaia, Adelle C.F. Coster, Cynthia Corley Mastick
Journal:
Biochemical Journal
Biochem J (2016) 473 (10): 1315–1327.
Published: 11 May 2016
...). published by Portland Press Limited on behalf of the Biochemical Society 2016 The Rab-GAP AS160 regulates glucose transport in adipocytes through two sequential insulin-sensitive steps. We show that Rab14 limits sorting of glucose transporter isoform 4 (Glut4) from endosomes into highly regulated...
Includes: Supplementary data
Articles
Fang Guo, Hui He, Zhi-Chao Fu, Shengping Huang, Tingtao Chen, Christopher J. Papasian, Leslie R. Morse, Yan Xu, Ricardo A. Battaglino, Xiao-Feng Yang, Zhisheng Jiang, Hong-Bo Xin, Mingui Fu
Journal:
Biochemical Journal
Biochem J (2015) 472 (3): 309–318.
Published: 27 November 2015
... that are associated with obesity-related metabolic complications. In an effort to identify novel proteins secreted from adipocytes that may negatively regulate macrophage inflammation, we found that peroxiredoxin (PRX)-like 2 activated in M-CSF stimulated monocytes (PAMM), a CXXC-type PRX-like 2 domain-containing...
Articles
Journal:
Biochemical Journal
Biochem J (2015) 468 (3): 425–434.
Published: 15 June 2015
... resistant to the allosteric Akt inhibitor MK-2206. We then developed adipocyte cell lines, in which endogenous Akt1 or Akt2 has been replaced by their corresponding drug-resistant Akt mutant. Treatment of those cells with MK-2206 allowed for acute and specific control of either Akt1 or Akt2 function. Our...
Includes: Supplementary data
Articles
Gerardo G. Piroli, Allison M. Manuel, Michael D. Walla, Matthew J. Jepson, Jonathan W. C. Brock, Mathur P. Rajesh, Ross M. Tanis, William E. Cotham, Norma Frizzell
Journal:
Biochemical Journal
Biochem J (2014) 462 (2): 231–245.
Published: 07 August 2014
... [S-(2-succino)cysteine]. We demonstrate that both α- and β-tubulin are increasingly modified by succination in 3T3-L1 adipocytes and in the adipose tissue of db / db mice. Incubation of purified tubulin from porcine brain with fumarate (50 mM) or the pharmacological compound DMF (dimethylfumarate...
Articles
Journal:
Biochemical Journal
Biochem J (2014) 459 (3): 489–503.
Published: 11 April 2014
...Miki Yuyama; Ko Fujimori VPA (valproic acid), a short-chain fatty acid that is a HDAC (histone deacetylase) inhibitor, is known to suppress adipogenesis. In the present study, we identified the molecular mechanism of VPA-mediated suppression of adipogenesis in adipocytes. VPA suppressed...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2013) 453 (2): 167–178.
Published: 28 June 2013
... of obesity, not all fat depots are created equal. Adipocytes found in white adipose tissue contain a single large lipid droplet and play well-known roles in energy storage. By contrast, brown adipose tissue is specialized for thermogenic energy expenditure. Owing to its significant capacity to dissipate...
Articles
Wulin Yang, Shermaine Thein, Xiangxiang Guo, Feng Xu, Byrappa Venkatesh, Shigeki Sugii, George K. Radda, Weiping Han
Journal:
Biochemical Journal
Biochem J (2013) 452 (1): 37–44.
Published: 25 April 2013
... LD (lipid droplet) dynamics and adipocyte differentiation, but whether these roles are mechanistically linked remains unclear. To understand how seipin regulates these processes, we investigated the evolutionary changes of seipin orthologues, and studied individual domains in regulating lipid...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2012) 445 (2): 247–254.
Published: 27 June 2012
...Norma Frizzell; Sonia A. Thomas; James A. Carson; John W. Baynes 2SC [ S -(2-succino)-cysteine] is a chemical modification formed by a Michael addition reaction of fumarate with cysteine residues in proteins. Formation of 2SC, termed ‘succination’ of proteins, increases in adipocytes grown in high...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2012) 445 (2): 265–273.
Published: 27 June 2012
... investigated N-glycosylation-deficient GLUT4 in detail in 3T3-L1 preadipocytes, 3T3-L1 adipocytes and L6 myoblasts. We have found no alterations in retention, insulin response, internalization or glucose transport activity. Degradation of the mutant molecule was increased, although once present at the cell...
Includes: Supplementary data
Articles
Graeme I. Lancaster, Beata Skiba, Christine Yang, Hayley T. Nicholls, Katherine G. Langley, M. H. Stanley Chan, Clinton R. Bruce, Gordon W. Rewcastle, Peter R. Shepherd, Michael Karin, Mark A. Febbraio
Journal:
Biochemical Journal
Biochem J (2012) 442 (3): 723–732.
Published: 24 February 2012
... in differentiated 3T3-L1 adipocytes. BMS-345541 did not prevent insulin-induced IRS1 serine phosphorylation on known IKKβ target sites. Secondly, adenovirus-mediated overexpression of wild-type IKKβ in differentiated 3T3-L1 adipocytes did not suppress insulin-stimulated 2-deoxyglucose uptake, IRS1 tyrosine...
Includes: Supplementary data
Articles
Roberta Possenti, Giampiero Muccioli, Pamela Petrocchi, Cheryl Cero, Aderville Cabassi, Lucy Vulchanova, Maureen S. Riedl, Monia Manieri, Andrea Frontini, Antonio Giordano, Saverio Cinti, Paolo Govoni, Gallia Graiani, Federico Quaini, Corrado Ghè, Elena Bresciani, Ilaria Bulgarelli, Antonio Torsello, Vittorio Locatelli, Valentina Sanghez, Bjarne D. Larsen, Jorgen S. Petersen, Paola Palanza, Stefano Parmigiani, Anna Moles, Andrea Levi, Alessandro Bartolomucci
Journal:
Biochemical Journal
Biochem J (2012) 441 (1): 511–522.
Published: 14 December 2011
... identification of a novel pro-lipolytic pathway mediated by the TLQP-21 peptide. We show for the first time that VGF-immunoreactivity is present within sympathetic fibres in the WAT (white adipose tissue) but not in the adipocytes. Furthermore, we identified a saturable receptor-binding activity for the TLQP-21...
Includes: Supplementary data
Articles
Xuefei Gao, Kuai Li, Xiaoyan Hui, Xiangping Kong, Gary Sweeney, Yu Wang, Aimin Xu, Maikun Teng, Pentao Liu, Donghai Wu
Journal:
Biochemical Journal
Biochem J (2011) 435 (3): 723–732.
Published: 13 April 2011
...Xuefei Gao; Kuai Li; Xiaoyan Hui; Xiangping Kong; Gary Sweeney; Yu Wang; Aimin Xu; Maikun Teng; Pentao Liu; Donghai Wu The adipocyte is the principal cell type for fat storage. CPT1 (carnitine palmitoyltransferase-1) is the rate-limiting enzyme for fatty acid β-oxidation, but the physiological role...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2011) 435 (2): 539–544.
Published: 29 March 2011
... allosteric Akt inhibitor under development for treating solid tumours. We report that MK-2206 potently inhibits Thr 308 Akt and Ser 473 Akt phosphorylation in 3T3-L1 adipocytes (IC 50 0.11 and 0.18 μM respectively) as well as downstream effects of insulin on GLUT4 (glucose transporter 4) translocation (IC 50...
Articles
Journal:
Biochemical Journal
Biochem J (2010) 432 (1): 191–198.
Published: 25 October 2010
... transporters to the plasma membrane. In the present study, we have investigated the metabolic side effects of non-ATP-competitive allosteric Akt inhibitors. In 3T3-L1 adipocytes, these inhibitors caused a decrease in the Akt signalling pathway concomitant with reduced glucose uptake. Surprisingly, a similar...
Includes: Supplementary data
Articles
Victoria A. Payne, Wo-Shing Au, Christopher E. Lowe, Shaikh M. Rahman, Jacob E. Friedman, Stephen O'Rahilly, Justin J. Rochford
Journal:
Biochemical Journal
Biochem J (2010) 425 (1): 215–224.
Published: 14 December 2009
... of adipocyte development including the induction of PPARγ (peroxisome-proliferator-activated receptor γ), the generation of an endogenous PPARγ ligand and the expression of several genes critical for lipid biosynthesis. Despite its significance, the regulation of SREBP1c expression during adipogenesis...
Includes: Supplementary data
Articles
Faiyaz Ahmad, Rebecka Lindh, Yan Tang, Iida Ruishalme, Anita Öst, Bobby Sahachartsiri, Peter Strålfors, Eva Degerman, Vincent C. Manganiello
Journal:
Biochemical Journal
Biochem J (2009) 424 (3): 399–410.
Published: 10 December 2009
...Faiyaz Ahmad; Rebecka Lindh; Yan Tang; Iida Ruishalme; Anita Öst; Bobby Sahachartsiri; Peter Strålfors; Eva Degerman; Vincent C. Manganiello In adipocytes, PDE3B (phosphodiesterase 3B) is an important regulatory effector in signalling pathways controlled by insulin and cAMP-increasing hormones...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2009) 419 (1): 105–113.
Published: 13 March 2009
... adipocyte cellugyrin glucose transporter 4 (GLUT4) protein chimaera syntaxin 6 vesicle Insulin stimulates glucose uptake in fat and skeletal muscle cells by redistributing GLUT4 (glucose transporter 4) from its insulin-responsive intracellular storage compartment(s) to the plasma membrane...
Includes: Supplementary data
Articles
Journal:
Biochemical Journal
Biochem J (2008) 411 (1): 89–95.
Published: 13 March 2008
... required for GLUT4 translocation. Based on their presence in GLUT4 vesicles and activity as AS160 GAP substrates, Rabs 8A, 8B, 10 and 14 are candidate Rabs. Here, we provide further evidence that Rab10 participates in GLUT4 translocation in 3T3-L1 adipocytes. Among Rabs 8A, 8B, 10 and 14, only...
Articles
Journal:
Biochemical Journal
Biochem J (2007) 403 (2): 243–250.
Published: 26 March 2007
... in a classical insulin-sensitive cell line, 3T3-L1 adipocytes. The results of the present study demonstrate that a specific pentacyclic triterpenoid, CG7, exerts an insulin-sensitizing effect as an IR activator in CHO/IR cells and adipocytes. The enhancement of insulin activity by CG7 may be useful...
Articles
Journal:
Biochemical Journal
Biochem J (2007) 403 (2): 353–358.
Published: 26 March 2007
... specificity of the Tbc1d1 GAP domain is identical with that of AS160. Ectopic expression of Tbc1d1 in 3T3-L1 adipocytes blocked insulin-stimulated GLUT4 translocation to the plasma membrane, whereas a point mutant with an inactive GAP domain had no effect. Insulin treatment led to the phosphorylation...
Articles
Mariko Omatsu-Kanbe, Kazuko Inoue, Yusuke Fujii, Takefumi Yamamoto, Takahiro Isono, Norihisa Fujita, Hiroshi Matsuura
Journal:
Biochemical Journal
Biochem J (2006) 393 (1): 171–180.
Published: 12 December 2005
...) in a concentration dependent manner. In this cell line, growth arrest is required before initiation of differentiation and growth-arrested post-confluent cells can be converted into adipocytes by the presence of the adipogenic hormones dexamethasone, 3-isobutyl-1-methylxanthine and insulin. On the other hand, those...
Articles
Journal:
Biochemical Journal
Biochem J (2006) 393 (1): 7–20.
Published: 12 December 2005
...Gema Frühbeck Leptin is a versatile 16 kDa peptide hormone, with a tertiary structure resembling that of members of the long-chain helical cytokine family. It is mainly produced by adipocytes in proportion to fat size stores, and was originally thought to act only as a satiety factor. However...
Articles
Journal:
Biochemical Journal
Biochem J (2003) 376 (3): 625–632.
Published: 15 December 2003
... in the development of type 2 diabetes. Inhibition of calpain activity has been shown to reduce insulinstimulated glucose uptake in isolated rat-muscle strips and adipocytes. In this report, we examine the mechanism by which calpain affects insulin-stimulated glucose uptake in 3T3-L1 adipocytes. Inhibition of calpain...
Articles
Journal:
Biochemical Journal
Biochem J (2002) 367 (1): 203–208.
Published: 01 October 2002
...-mail [email protected] ). 21 2 2002 8 6 2002 3 7 2002 3 7 2002 The Biochemical Society, London ©2002 2002 adipocyte daidzein peroxisome-proliferator-activated receptor γ (PPARγ) preadipocyte Abbreviations used: ALLN, N -acetyl-Leu-Leu-norleucinal; C/EBP, CCAAT...
Articles
Pavel FLACHS, JiŘí NOVOTNÝ, Filip BAUMRUK, Kristina BARDOVÁ, Lenka BOUŘOVÁ, Ivan MIKŠÍK, Jana ŠPONAROVÁ, Petr SVOBODA, Jan KOPECKÝ
Journal:
Biochemical Journal
Biochem J (2002) 364 (2): 369–376.
Published: 01 June 2002
...Pavel FLACHS; JiŘí NOVOTNÝ; Filip BAUMRUK; Kristina BARDOVÁ; Lenka BOUŘOVÁ; Ivan MIKŠÍK; Jana ŠPONAROVÁ; Petr SVOBODA; Jan KOPECKÝ In vitro experiments suggest that stimulation of lipolysis by catecholamines in adipocytes depends on the energy status of these cells. We tested whether mitochondrial...
Articles
Journal:
Biochemical Journal
Biochem J (2001) 360 (2): 305–312.
Published: 26 November 2001
... by LCFAs and thiazolidinediones in pre-adipocytes via a peroxisome-proliferator-activated receptor (PPAR)-mediated process. We isolated and analysed the murine FAT/CD36 promoter employing C 2 C 12 N cells directed to differentiate to either adipose or muscle. Transient transfection studies revealed...
Articles
Journal:
Biochemical Journal
Biochem J (2001) 358 (2): 335–342.
Published: 24 August 2001
... and adipocytes. Recent studies have suggested that SSAO could activate glucose transport in fat cells. In the present work, we investigated the potential role of a chronic SSAO activation on adipocyte maturation of the 3T3-L1 pre-adipose cell line. Exposure of post-confluent 3T3-L1 pre-adipocytes to methylamine...
Articles
Journal:
Biochemical Journal
Biochem J (2001) 356 (1): 143–149.
Published: 08 May 2001
...Mireille CORMONT; Nadine GAUTIER; Karine ILC; Yannick Le MARCHAND-BRUSTEL The small GTPase Rab4 has been shown to participate in the subcellular distribution of GLUT4 under both basal and insulin-stimulated conditions in adipocytes. In the present work, we have characterized the effect of Rab4 ΔCT...
Articles
Journal:
Biochemical Journal
Biochem J (2000) 350 (1): 99–107.
Published: 09 August 2000
...Annette M. SHEWAN; Brad J. MARSH; Derek R. MELVIN; Sally MARTIN; Gwyn W. GOULD; David E. JAMES The insulin-responsive glucose transporter GLUT4 is targeted to a post-endocytic compartment in adipocytes, from where it moves to the cell surface in response to insulin. Previous studies have identified...
Articles
Rosa ALVAREZ, MaLuz CHECA, Sonia BRUN, Octavi VI±AS, Teresa MAMPEL, Roser IGLESIAS, Marta GIRALT, Francesc VILLARROYA
Journal:
Biochemical Journal
Biochem J (2000) 345 (1): 91–97.
Published: 17 December 1999
... transcription of ucp-1 and the RA receptor (RAR) is known to be involved in this effect. However, co-transfection of an expression vector for retinoid-X receptor (RXR) increases the action of 9- cis RA but not the effects of all- trans RA on the ucp-1 promoter in brown adipocytes. Either RAR-specific { p-[(E)-2...
Articles
Kallol DAS, Renée Y. LEWIS, Terry P. COMBATSIARIS, Ying LIN, Lawrence SHAPIRO, Maureen J. CHARRON, Philipp E. SCHERER
Journal:
Biochemical Journal
Biochem J (1999) 344 (2): 313–320.
Published: 24 November 1999
... at very high levels in mitochondria of white adipocytes and is strongly induced in the course of 3T3-L1 adipogenesis. To determine the consequences of the presence of mDIC on the mitochondrial membrane potential, we transiently expressed mDIC in 293-T cells. Overexpression of mDIC leads to significant...
Articles
Kate A. POWELL, Lachlan C. CAMPBELL, Jeremy M. TAVARÉ, David P. LEADER, Jill A. WAKEFIELD, Gwyn W. GOULD
Journal:
Biochemical Journal
Biochem J (1999) 344 (2): 535–543.
Published: 24 November 1999
... membrane. We have engineered a series of chimaeras between Glut4 and green fluorescent protein (GFP) from Aequoria victoria and expressed these proteins in 3T3-L1 adipocytes by microinjection of plasmid cDNA. In the absence of insulin, GFP-Glut4 is localized intracellularly within a perinuclear compartment...