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Keywords: adipocyte
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Adipose biology
Biochem J (2020) 477 (5): 985–1008.
Published: 13 March 2020
...Alexander Yang; Emilio P. Mottillo Fatty acids (FAs) are stored safely in the form of triacylglycerol (TAG) in lipid droplet (LD) organelles by professional storage cells called adipocytes. These lipids are mobilized during adipocyte lipolysis, the fundamental process of hydrolyzing TAG to FAs...
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Biochem J (2015) 472 (3): 309–318.
Published: 27 November 2015
... that are associated with obesity-related metabolic complications. In an effort to identify novel proteins secreted from adipocytes that may negatively regulate macrophage inflammation, we found that peroxiredoxin (PRX)-like 2 activated in M-CSF stimulated monocytes (PAMM), a CXXC-type PRX-like 2 domain-containing...
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Biochem J (2015) 468 (3): 425–434.
Published: 15 June 2015
... resistant to the allosteric Akt inhibitor MK-2206. We then developed adipocyte cell lines, in which endogenous Akt1 or Akt2 has been replaced by their corresponding drug-resistant Akt mutant. Treatment of those cells with MK-2206 allowed for acute and specific control of either Akt1 or Akt2 function. Our...
Includes: Supplementary data
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Biochem J (2014) 462 (2): 231–245.
Published: 07 August 2014
... [S-(2-succino)cysteine]. We demonstrate that both α- and β-tubulin are increasingly modified by succination in 3T3-L1 adipocytes and in the adipose tissue of db / db mice. Incubation of purified tubulin from porcine brain with fumarate (50 mM) or the pharmacological compound DMF (dimethylfumarate...
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Biochem J (2014) 459 (3): 489–503.
Published: 11 April 2014
...Miki Yuyama; Ko Fujimori VPA (valproic acid), a short-chain fatty acid that is a HDAC (histone deacetylase) inhibitor, is known to suppress adipogenesis. In the present study, we identified the molecular mechanism of VPA-mediated suppression of adipogenesis in adipocytes. VPA suppressed...
Includes: Supplementary data
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Biochem J (2013) 453 (2): 167–178.
Published: 28 June 2013
... of obesity, not all fat depots are created equal. Adipocytes found in white adipose tissue contain a single large lipid droplet and play well-known roles in energy storage. By contrast, brown adipose tissue is specialized for thermogenic energy expenditure. Owing to its significant capacity to dissipate...
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Biochem J (2012) 445 (2): 247–254.
Published: 27 June 2012
...Norma Frizzell; Sonia A. Thomas; James A. Carson; John W. Baynes 2SC [ S -(2-succino)-cysteine] is a chemical modification formed by a Michael addition reaction of fumarate with cysteine residues in proteins. Formation of 2SC, termed ‘succination’ of proteins, increases in adipocytes grown in high...
Includes: Supplementary data
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Biochem J (2012) 445 (2): 265–273.
Published: 27 June 2012
... investigated N-glycosylation-deficient GLUT4 in detail in 3T3-L1 preadipocytes, 3T3-L1 adipocytes and L6 myoblasts. We have found no alterations in retention, insulin response, internalization or glucose transport activity. Degradation of the mutant molecule was increased, although once present at the cell...
Includes: Supplementary data
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Biochem J (2012) 442 (3): 723–732.
Published: 24 February 2012
... in differentiated 3T3-L1 adipocytes. BMS-345541 did not prevent insulin-induced IRS1 serine phosphorylation on known IKKβ target sites. Secondly, adenovirus-mediated overexpression of wild-type IKKβ in differentiated 3T3-L1 adipocytes did not suppress insulin-stimulated 2-deoxyglucose uptake, IRS1 tyrosine...
Includes: Supplementary data
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Biochem J (2011) 435 (2): 539–544.
Published: 29 March 2011
... allosteric Akt inhibitor under development for treating solid tumours. We report that MK-2206 potently inhibits Thr 308 Akt and Ser 473 Akt phosphorylation in 3T3-L1 adipocytes (IC 50 0.11 and 0.18 μM respectively) as well as downstream effects of insulin on GLUT4 (glucose transporter 4) translocation (IC 50...
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Biochem J (2010) 432 (1): 191–198.
Published: 25 October 2010
... transporters to the plasma membrane. In the present study, we have investigated the metabolic side effects of non-ATP-competitive allosteric Akt inhibitors. In 3T3-L1 adipocytes, these inhibitors caused a decrease in the Akt signalling pathway concomitant with reduced glucose uptake. Surprisingly, a similar...
Includes: Supplementary data
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Biochem J (2010) 425 (1): 215–224.
Published: 14 December 2009
... of adipocyte development including the induction of PPARγ (peroxisome-proliferator-activated receptor γ), the generation of an endogenous PPARγ ligand and the expression of several genes critical for lipid biosynthesis. Despite its significance, the regulation of SREBP1c expression during adipogenesis...
Includes: Supplementary data
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Biochem J (2009) 419 (1): 105–113.
Published: 13 March 2009
... adipocyte cellugyrin glucose transporter 4 (GLUT4) protein chimaera syntaxin 6 vesicle Insulin stimulates glucose uptake in fat and skeletal muscle cells by redistributing GLUT4 (glucose transporter 4) from its insulin-responsive intracellular storage compartment(s) to the plasma membrane...
Includes: Supplementary data
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Biochem J (2008) 411 (1): 89–95.
Published: 13 March 2008
... required for GLUT4 translocation. Based on their presence in GLUT4 vesicles and activity as AS160 GAP substrates, Rabs 8A, 8B, 10 and 14 are candidate Rabs. Here, we provide further evidence that Rab10 participates in GLUT4 translocation in 3T3-L1 adipocytes. Among Rabs 8A, 8B, 10 and 14, only...
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Biochem J (2007) 403 (2): 243–250.
Published: 26 March 2007
... in a classical insulin-sensitive cell line, 3T3-L1 adipocytes. The results of the present study demonstrate that a specific pentacyclic triterpenoid, CG7, exerts an insulin-sensitizing effect as an IR activator in CHO/IR cells and adipocytes. The enhancement of insulin activity by CG7 may be useful...
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Biochem J (2007) 403 (2): 353–358.
Published: 26 March 2007
... specificity of the Tbc1d1 GAP domain is identical with that of AS160. Ectopic expression of Tbc1d1 in 3T3-L1 adipocytes blocked insulin-stimulated GLUT4 translocation to the plasma membrane, whereas a point mutant with an inactive GAP domain had no effect. Insulin treatment led to the phosphorylation...
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Biochem J (2006) 393 (1): 7–20.
Published: 12 December 2005
...Gema Frühbeck Leptin is a versatile 16 kDa peptide hormone, with a tertiary structure resembling that of members of the long-chain helical cytokine family. It is mainly produced by adipocytes in proportion to fat size stores, and was originally thought to act only as a satiety factor. However...
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Biochem J (2003) 376 (3): 625–632.
Published: 15 December 2003
... in the development of type 2 diabetes. Inhibition of calpain activity has been shown to reduce insulinstimulated glucose uptake in isolated rat-muscle strips and adipocytes. In this report, we examine the mechanism by which calpain affects insulin-stimulated glucose uptake in 3T3-L1 adipocytes. Inhibition of calpain...
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Biochem J (2001) 358 (2): 335–342.
Published: 24 August 2001
... and adipocytes. Recent studies have suggested that SSAO could activate glucose transport in fat cells. In the present work, we investigated the potential role of a chronic SSAO activation on adipocyte maturation of the 3T3-L1 pre-adipose cell line. Exposure of post-confluent 3T3-L1 pre-adipocytes to methylamine...
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Biochem J (1999) 344 (2): 313–320.
Published: 24 November 1999
... at very high levels in mitochondria of white adipocytes and is strongly induced in the course of 3T3-L1 adipogenesis. To determine the consequences of the presence of mDIC on the mitochondrial membrane potential, we transiently expressed mDIC in 293-T cells. Overexpression of mDIC leads to significant...
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