...Sophia Bustraan; Jane Bennett; Chad Whilding; Betheney R. Pennycook; David Smith; Alexis R. Barr; Jon Read; David Carling; Alice Pollard Adipogenesis, defined as the development of mature adipocytes from stem cell precursors, is vital for the expansion, turnover and health of adipose tissue. Loss...

... that plays an important role in regulating whole-body energy homeostasis. Using the well-established mouse 3T3-L1 in vitro model of adipogenesis, the role of the MNKs in adipocyte differentiation and lipid storage was investigated. Inhibition of MNK activity using specific inhibitors failed to impair...

... and/or targeting reagents. Here, we discuss several receptor families with an important function in adipogenesis and mature adipocytes to highlight the complexity at the cell surface and illustrate the problems with identifying adipose selective proteins. We then discuss that, while no unique adipocyte surface...

... on adipogenesis. AMPKβ2 was the principal AMPKβ isoform in 3T3-L1 adipocytes, isolated rodent adipocytes and human subcutaneous adipose tissue, as assessed by the contribution to total cellular AMPK activity. Down-regulation of AMPKβ2 with siRNA inhibited lipid accumulation, cellular adiponectin levels...

... and CCAAT–enhancer-binding protein (C/EBP) α, β, and δ, are important for the process, whereas the stage-specific intracellular signal transduction regulating the onset of adipogenesis remains enigmatic. Here, we explored the functional role of c- jun N-terminal kinases (JNKs) in adipogenic differentiation...

... transmission by this pivotal kinase. 1 To whom correspondence should be addressed (email [email protected] ). 2 7 2015 6 7 2015 6 7 2015 © 2015 Authors; published by Portland Press Limited 2015 adipogenesis forkhead box O1 (FoxO1) glucose transporter (GLUT) 4...

... factor forkhead box O1 (FoxO1) and the glucose transporter 4 (GLUT4), revealing a redundant role for these Akt kinases in the control of glucose transport into fat cells. In contrast, Akt1 signalling is uniquely required for adipogenesis, by controlling the mitotic clonal expansion (MCE) of pre...

... . Interestingly, the mixture elicited conversion of NIH-3T3 cells directly into adipocytes without a preceding pre-adipocyte stage. Functional analysis revealed that each component of the mixture was necessary for the induction of adipogenesis, including Dex which inhibited the cell proliferation stimulated...

... cofilin is a requisite step in adipogenesis. However, it remains unclear whether actin nucleation and assembly into the cortical structure are essential for adipocyte development. In the present study we investigated the role of cortical actin assembly and of actin nucleation by the actin-related protein...

...Dongju Jung; Lutfi Abu-Elheiga; Rie Ayuzawa; Ziwei Gu; Takashi Shirakawa; Yukio Fujiki; Norio Nakatsuji; Salih J. Wakil; Motonari Uesugi Chromeceptin is a synthetic small molecule that inhibits insulin-induced adipogenesis of 3T3-L1 cells and impairs the function of IGF2 (insulin-like growth factor...

... preadipocyte differentiation. During both overexpression and under physiological conditions, CREBL2 interacted and was entirely co-localized with CREB. Overexpression of CREBL2 was sufficient to promote adipogenesis via up-regulating the expression of PPARγ (peroxisome-proliferator-activated receptor γ) and C...

... differentiation. 1 These authors contributed equally to this work. 2 To whom correspondence should be addressed (email [email protected] ). 17 9 2010 24 2 2011 4 3 2011 4 3 2011 © The Authors Journal compilation © 2011 Biochemical Society 2011 adipogenesis...

... compilation © 2010 Biochemical Society 2010 adipogenesis fetuin-A insulin resistance nonesterified (‘free’) fatty acid nuclear factor κB (NF-κB) Type 2 diabetes Table 1 Primers used in the present study Primer Direction Sequence NF-κBp65 Forward 5...

... in response to PPARγ (peroxisome-proliferator-activated receptor γ), an inducer of adipogenesis. We show that the human AACS promoter is a PPARγ target gene and that this nuclear receptor is recruited to the AACS promoter by direct interaction with Sp1 (stimulating protein-1). 1 These authors...

.... Brd2 is highly expressed in pancreatic β-cells, where it normally inhibits β-cell mitosis and insulin transcription. In 3T3-L1 pre-adipocytes, Brd2 normally co-represses PPAR-γ (peroxisome-proliferator-activated receptor-γ) and inhibits adipogenesis. Brd2 knockdown protects 3T3-L1 adipocytes from TNF-α...

... of adipocyte development including the induction of PPARγ (peroxisome-proliferator-activated receptor γ), the generation of an endogenous PPARγ ligand and the expression of several genes critical for lipid biosynthesis. Despite its significance, the regulation of SREBP1c expression during adipogenesis...

... that are involved in numerous processes, including lipid metabolism and adipogenesis. By comparing liver mRNAs of wild-type and PPARα-null mice using microarrays, a novel putative target gene of PPARα, G0S2 (G 0 /G 1 switch gene 2), was identified. Hepatic expression of G0S2 was up-regulated by fasting...