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Keywords: aequorin
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Biochem J (2015) 466 (3): 455–465.
Published: 06 March 2015
...Francisco J. Aulestia; María Teresa Alonso; Javier García-Sancho High Ca 2+ content in the Golgi apparatus (Go) is essential for protein processing and sorting. In addition, the Go can shape the cytosolic Ca 2+ signals by releasing or sequestering Ca 2+ . We generated two new aequorin-based Ca 2...
Includes: Multimedia, Supplementary data
Biochem J (2014) 464 (1): e5–e6.
Published: 23 October 2014
... 10.1113/jphysiol.2013.268235 24366263 2 Bonora M. Giorgi C. Bononi A. Marchi S. Patergnani S. Rimessi A. Rizzuto R. Pinton P. Subcellular calcium measurements in mammalian cells using jellyfish photoprotein aequorin-based probes Nat. Protoc. 2013 8 2105 2118...
Biochem J (2014) 458 (1): 33–40.
Published: 20 January 2014
... expressing either the MICU1-specific shRNA or scrambled shRNA were also obtained and used in some of the experiments. To measure the [Ca 2+ ] M in the transiently transfected cells, the plasmids containing either single- or double-mutated aequorin were co-transfected with the shRNA construct. Cells co...
Includes: Supplementary data
Biochem J (2012) 445 (3): 371–376.
Published: 13 July 2012
... millimolar levels in a few seconds. Measuring the dynamics of [Ca 2+ ] M during prolonged stimulation has been previously precluded by the high Ca 2+ affinity of the probes available. We have now developed a mitochondrially targeted double-mutated form of the photoprotein aequorin which is able to measure...
Biochem J (2011) 437 (3): 469–475.
Published: 13 July 2011
... 40)] cells by using targeted aequorins for selective monitorization of Ca 2+ transport by organelles. We find that CALHM1 increases Ca 2+ leak from the ER and, more importantly, reduces ER Ca 2+ uptake by decreasing both the transport capacity and the Ca 2+ affinity of SERCA (sarcoplasmic/endoplasmic...
Includes: Supplementary data
Biochem J (2011) 435 (1): 227–235.
Published: 15 March 2011
... Ca 2+ stores using targeted aequorins for selective measurements in different subcellular compartments. Both, HEK-293T [HEK (human embryonic kidney)-293 cells expressing the large T-antigen of SV40 (simian virus 40)] and HeLa cells accumulated Ca 2+ into the ER (endoplasmic reticulum) to near...
Includes: Supplementary data
Biochem J (2006) 395 (2): 267–276.
Published: 28 March 2006
... be responsible for the degeneration of muscle occurring in DMD. In the present study, we used subsarcolemmal- and mitochondrial-targeted aequorin to study the effect of the antiapoptotic Bcl-2 protein overexpression on carbachol-induced near-plasma membrane and mitochondrial calcium responses in myotubes derived...
Biochem J (2002) 365 (2): 451–459.
Published: 15 July 2002
...% of the mitochondrial space to several hundred micromolar in 2–3% of mitochondria. Intermediate levels were found in the rest of the mitochondrial space. Single-cell imaging experiments with aequorin showed that the heterogeneity had both an intercellular and a subcellular origin. Those mitochondria responding...
Biochem J (2001) 353 (2): 175–180.
Published: 08 January 2001
... the importance of this phenomenon for nutrient sensing in pancreatic islets and β-cells by imaging adenovirally expressed Ca 2+ and ATP sensors (aequorin and firefly luciferase). [Ca 2+ ] m increases provoked by KCl or tolbutamide evoked an immediate increase in cytosolic and mitochondrial free ATP concentration...
Biochem J (1999) 340 (1): 33–40.
Published: 10 May 1999
...Evelyn L. RIDGLEY; Zhao-hui XIONG; Larry RUBEN Here we examine a cell death process induced by reactive oxygen species (ROS) in the haemoflagellate Trypanosoma brucei brucei . Ca 2+ distribution in cellular compartments was measured with stable transformants expressing aequorin targeted...