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Keywords: allosteric regulation
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Biochem J (2024) 481 (6): 437–460.
Published: 13 March 2024
... HXMS hydrogen/deuterium exchange mass spectrometry NAD + nicotinamide adenine dinucleotide PAR poly(ADP-ribose) PARP poly (ADP-ribose) polymerase allosteric regulation crystallography HXMS PARP veliparib analogs References 1 De Vos , M...
Includes: Supplementary data
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Biochem J (2023) 480 (19): 1503–1532.
Published: 04 October 2023
... ]. However, under in vitro experimental conditions, the polypeptide substrate derived from the pseudosubstrate region can also displace the autoinhibitory domains and underestimate the proportion of autoinhibited kinase [ 30 ]. AGC kinase allosteric regulation PDPK1 phosphorylation...
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Biochem J (2023) 480 (10): 715–728.
Published: 19 May 2023
...), in addition to the technological advances that have facilitated the study of a multitude of functions. Table 1. Methods for functional analysis of pseudoenzymes Mode of action Family Examples Methods of study References Allosteric regulation Pseudokinase Pseudokinase FAM20A...
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Biochem J (2022) 479 (7): 825–838.
Published: 11 April 2022
.... Correspondence: Giuseppe Melacini ( melacin@mcmaster.ca ) 21 11 2021 17 3 2022 21 3 2022 © 2022 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2022 agonism allosteric regulation antagonism cAMP kinases protein kinase A Allosteric...
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Biochem J (2020) 477 (17): 3387–3399.
Published: 17 September 2020
... ) allosteric regulation dimerization drug discovery pseudokinase RNase L l sunitinib Light scattering experiments were performed on a DynaPro NanoStar instrument (Wyatt Technology Corporation) at 25°C. The scattered light was collected at an angle of 90°. Due to that 0.2–10 mg/ml protein...
Includes: Supplementary data
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Biochem J (2020) 477 (7): 1203–1218.
Published: 02 April 2020
... Society and distributed under the Creative Commons Attribution License 4.0 (CC BY-NC-ND) . allosteric regulation GTPases leucine-rich repeat kinase Nanobody Roco proteins form a family of large multi-domain proteins, that are characterized by the occurrence of a RocCOR supra-domain...
Includes: Supplementary data
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Biochem J (2019) 476 (20): 2939–2952.
Published: 18 October 2019
... enzymes exhibited high affinity for oxaloacetate and ATP, reinforcing their role as decarboxylases. We employed a high-throughput screening for putative allosteric regulators using differential scanning fluorometry and confirmed their effect on enzyme activity by performing enzyme kinetics. Ath PEPCK1...
Includes: Supplementary data
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Biochem J (2018) 475 (12): 2025–2042.
Published: 26 June 2018
... of the spindle, where it plays important roles in the assembly and function of the bipolar spindle [ 12 ]. allosteric regulation aurora kinases protein dynamics The presence of a polar residue in the usually hydrophobic back pocket of some kinase active sites has important implications...
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Biochem J (2018) 475 (6): 1141–1158.
Published: 26 March 2018
... (PerkinElmer), and then performing liquid scintillation counting with a 1215 Rackbeta instrument (LKB Wallac). © 2018 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2018 allosteric regulation cation transporter enzyme kinetics membrane-bound...
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Biochem J (2018) 475 (1): 247–260.
Published: 05 January 2018
..., which differ in their molecular architecture and mechanism of allosteric regulation. The short-form ATP-PRT is a hetero-octamer, with four HisG chains that comprise only the catalytic domains and four separate chains of HisZ required for allosteric regulation by histidine. The long-form ATP-PRT is homo...
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Biochem J (2017) 474 (17): 2977–2980.
Published: 17 August 2017
... transfer NMR spectroscopy . J. Am. Chem. Soc. 129 , 1743 – 1750 doi: 10.1021/ja067827z allosteric regulation calcium signaling entropy NMR spectroscopy It has long been known that calcium signaling is a centerpiece for intracellular processes. The S100 proteins contain two EF-hand...
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Biochem J (2017) 474 (7): 1307–1320.
Published: 23 March 2017
... found out that phosphatidylglycerol and sulfoquinovosyl diacylglycerol are allosteric regulators of the nucleotide binding, which increase the affinity towards NADPH a 100-fold. Moreover, we showed for the first time that NADH can, like NADPH, form active complexes with Pchlide and POR, however, at much...
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Biochem J (2017) 474 (5): 781–795.
Published: 20 February 2017
... conformations may be low, an allosteric event is capable of enhancing them through a ‘population shift’. Below, we illustrate this to be the case, with the allosteric regulator being a covalently linked Nedd8 molecule. Post-translational modifications are known to act allosterically [ 21 ]. © 2017...
Includes: Supplementary data
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Biochem J (2016) 473 (15): 2273–2293.
Published: 28 July 2016
... on behalf of the Biochemical Society 2016 allosteric regulation heparin metastability polymerization proteinase inhibition protein folding receptor binding serpin The initial identification of a relationship that would grow into the serpin superfamily of proteins was made in 1980...
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Biochem J (2016) 473 (9): 1165–1178.
Published: 26 April 2016
... glutamine. It is subject to allosteric regulation by NADH and the ATP/ADP ratio, and by Ca 2+ through binding to the E1 subunit. The latter involves a unique Ca 2+ -binding site which includes D 114 ADLD (site 1). Here, we describe three splice variants of E1 in which either the exon expressing this site...
Includes: Supplementary data
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Biochem J (2016) 473 (8): 1027–1035.
Published: 08 April 2016
... inhibitor which significantly reduced the viability of triple negative breast cancer (TNBC) cells, but was less toxic to normal fibroblasts. allosteric regulation breast cancer inhibitor kinesin mitosis Inhibition of cell mitosis is a clinically validated strategy for cancer treatment [ 1...
Includes: Supplementary data
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Biochem J (2014) 464 (3): 343–354.
Published: 05 December 2014
... a cytoplasmic interaction between Kir6 and SUR subunits of ATP-sensitive potassium channels. We identify residues forming a key salt-bridge controlling transfer of allosteric information from agonist/antagonist binding to gating of the channel pore. allosteric regulation glibenclamide inward rectifier...
Includes: Supplementary data
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Biochem J (2010) 430 (2): 179–189.
Published: 13 August 2010
... for interfering with the catalytic machinery of proteases. allosteric regulation antibody inhibition mechanism phage display pseudo-protease serine protease Proteases hydrolyse peptide bonds of protein and peptide substrates, an activity which is fundamental to many aspects of human physiology...
Includes: Multimedia, Supplementary data
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Biochem J (2009) 420 (2): 201–209.
Published: 13 May 2009
.... In the present study, we decipher the functional role of the residue Lys 57 , which resides at the fumarate-binding site and dimer interface, and thus may be involved in the allosteric regulation and subunit–subunit interaction of the enzyme. In the present study, Lys 57 is replaced with alanine, cysteine...
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Biochem J (2005) 391 (3): 449–464.
Published: 25 October 2005
...; indeed, a handful of co-repressors may be sufficient to accurately fulfil the task in hand. AF1 transactivation domain allosteric regulation protein–nucleic acid interaction protein–protein interaction post-translational modification secondary structure steroid receptor The AF1 domain...
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Biochem J (2003) 376 (1): 277–283.
Published: 15 November 2003
... ©2003 2003 allosteric regulation eosin-5-maleimide phosphofructokinase-2 pyrene N-(1-pyrenyl) maleimide SH group Biochem. J. (2003) 376, 277 283 (Printed in Great Britain) 277 Structural and functional roles of Cys-238 and Cys-295 in Escherichia coli phosphofructokinase-2 Mauricio BAEZ...
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Biochem J (2001) 359 (3): 591–597.
Published: 25 October 2001
...} phosphatase activity ratio by 4-fold, and decreased the affinity for the substrate fructose 6-phosphate. The data show that the N-terminus is important both for subunit assembly and for defining the kinetic properties of the enzyme. Key words: allosteric regulation, enzyme kinetics, fructose-2,6- bisphosphate...
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Biochem J (1999) 341 (1): 15–23.
Published: 24 June 1999
... of the well-known prokaryotic enzyme. Key words: allosteric regulation, glycolysis, mutagenesis in Šitro, PFK genes, yeast metabolism. sequenced [14,15]. Promoter studies revealed that PFK1 and PFK2 are constitutively transcribed at a relatively high level in S. cereŠisiae [16]. From the deduced amino acid...