1-50 of 53
Keywords: alternative splicing
Close
Sort by
Articles
Biochem J (2023) 480 (5): 385–401.
Published: 15 March 2023
... study, we identified two Doublesex ( Dsx )-like mRNA isoforms in the brine shrimp Artemia franciscana (Kellogg 1906), which are generated by the combination of alternative promoters, alternative splicing and alternative polyadenylation. The two transcripts exhibited sex-biased enrichment, which we...
Includes: Supplementary data
Articles
Biochem J (2020) 477 (16): 3091–3104.
Published: 28 August 2020
...Luciana E. Giono; Alberto R. Kornblihtt Gene expression is an intricately regulated process that is at the basis of cell differentiation, the maintenance of cell identity and the cellular responses to environmental changes. Alternative splicing, the process by which multiple functionally distinct...
Articles
Articles
Biochem J (2017) 474 (6): 885–896.
Published: 07 March 2017
...Sathiya Pandi Narayanan; Smriti Singh; Sanjeev Shukla The discovery of an increasing number of alternative splicing events in the human genome highlighted that ∼94% of genes generate alternatively spliced transcripts that may produce different protein isoforms with diverse functions. It is now well...
Articles
Biochem J (2016) 473 (12): 1693–1702.
Published: 10 June 2016
... and Complete™ protease inhibitor cocktail). Finally, beads were incubated with Laemmli sample buffer containing 5% 2-mercaptoethanol and heated at 95°C for 10 min to elute proteins. alternative splicing glycobiology mitochondria O-GlcNAc transferase (OGT) O-linked N -acetylglucosamine (O-GlcNAc...
Articles
Biochem J (2015) 468 (2): 191–202.
Published: 22 May 2015
... To whom correspondence should be addressed (email [email protected] ). 3 3 2015 16 3 2015 © The Authors Journal compilation © 2015 Biochemical Society 2015 alternative splicing arginine-demethylase epigenetic regulation Fe(II) and 2-oxoglutarate dependent...
Articles
Biochem J (2014) 458 (3): 513–523.
Published: 28 February 2014
... (cysteinyl-tRNA synthetase). We demonstrate that the single Pf CysRS ( Plasmodium falciparum CysRS) transcript is alternatively spliced, and, using a combination of endogenous and heterologous tagging experiments in both P. falciparum and Toxoplasma gondii , we show that CysRS isoforms traffic to the cytosol...
Includes: Multimedia, Supplementary data
Articles
Biochem J (2013) 453 (2): 271–279.
Published: 28 June 2013
...Ganesh Shankarling; Kristen W. Lynch Understanding functional distinctions between related splicing regulatory proteins is critical to deciphering tissue-specific control of alternative splicing. The hnRNP (heterogeneous nuclear ribonucleoprotein) L and hnRNP LL (hnRNP L-like) proteins...
Articles
Biochem J (2013) 450 (1): 149–157.
Published: 24 January 2013
...Abdel A. Belaidi; Guenter Schwarz The complexity of eukaryotic multicellular organisms relies on evolutionary developments that include compartmentalization, alternative splicing, protein domain fusion and post-translational modification. Mammalian gephyrin uniquely exemplifies these processes...
Includes: Supplementary data
Articles
Biochem J (2013) 450 (1): 85–94.
Published: 24 January 2013
...Deshun Gong; Fan Yang; Fudong Li; Dandan Qian; Minhao Wu; Zhenhua Shao; Mian Wu; Jihui Wu; Yunyu Shi Human RBM25 (RNA-binding motif protein 25) is a novel splicing factor that contains a PWI domain, a newly identified RNA/DNA-binding domain, and regulates Bcl-x pre-mRNA alternative splicing...
Includes: Supplementary data
Articles
Articles
Biochem J (2012) 445 (2): 145–156.
Published: 27 June 2012
...Hegel R. Hernandez-Lopez; Sheila V. Graham Persistent infection with cancer risk-related viruses leads to molecular, cellular and immune response changes in host organisms that in some cases direct cellular transformation. Alternative splicing is a conserved cellular process that increases...
Articles
Biochem J (2012) 442 (3): 573–581.
Published: 24 February 2012
... of the interaction domain in DREB2A appears to be required for proper DREB2A function under stress conditions. abiotic stress alternative splicing CMIV-3 domain protein–protein interaction protein stability transcription factor Protein structure predictions for the RST domain of RCD1 and for the C...
Includes: Supplementary data
Articles
Articles
Biochem J (2010) 428 (3): 347–358.
Published: 27 May 2010
... 2010 26 4 2010 26 4 2010 © The Authors Journal compilation © 2010 Biochemical Society 2010 alternative splicing DNA cloning vascular endothelial growth factor-C (VEGF-C) vascular endothelial growth factor-C isoform 62 (VEGF-C 62 ) vascular endothelial growth factor-C isoform...
Includes: Supplementary data
Articles
Biochem J (2010) 427 (3): 377–390.
Published: 14 April 2010
... alternative splicing from three exons. These exon 1 variants were designated exons 1A, 1B and 1C on the basis of their 5′–3′ order. RT (reverse transcription)–PCR demonstrates the differential expression in various human tissues. The alternative 5′-UTRs (untranslated regions) possessed by these isoforms have...
Includes: Supplementary data
Articles
Biochem J (2009) 422 (2): 321–328.
Published: 13 August 2009
...) and to regulate the alternative splicing of SSAT pre-mRNA. The unique effects of chiral polyamine analogues on polyamine metabolism may offer novel possibilities for studying the physiological functions, control mechanisms, and targets of the natural polyamines, as well as advance therapeutic drug development...
Articles
Articles
Biochem J (2009) 417 (1): 15–27.
Published: 12 December 2008
... and are also important regulators of alternative splicing. In addition they participate in post-splicing activities, such as mRNA nuclear export, nonsense-mediated mRNA decay and mRNA translation. These wide-ranging roles of SR proteins highlight their importance as pivotal regulators of mRNA metabolism...
Articles
Biochem J (2008) 416 (1): e1–e3.
Published: 28 October 2008
... (AMP-activated protein kinase)-related kinases which control cell metabolism, cell growth and cell polarity. In this issue of the Biochemical Journal , Hardie and colleagues discover an alternative splice form of LKB1 that alters the C-terminus of the protein containing a few known sites of post...
Articles
Biochem J (2008) 414 (1): 121–131.
Published: 29 July 2008
... different hH 3 R (human H 3 R) isoforms led us to investigate the possible existence of H 4 R splice variants. In the present paper, we report on the cloning of the first two alternatively spliced H 4 R isoforms from CD34+ cord blood-cell-derived eosinophils and mast cells. These H 4 R splice variants...
Includes: Supplementary data
Articles
Biochem J (2007) 405 (1): 21–30.
Published: 13 June 2007
...Zhaohua Tang; Amy Tsurumi; Sarah Alaei; Christopher Wilson; Cathleen Chiu; Jessica Oya; Benson Ngo Evolutionarily conserved SR proteins (serine/arginine-rich proteins) are important factors for alternative splicing and their activity is modulated by SRPKs (SR protein-specific kinases). We...
Articles
Biochem J (2007) 401 (1): 185–195.
Published: 11 December 2006
...Chiharu Sogawa; Kei Kumagai; Norio Sogawa; Katsuya Morita; Toshihiro Dohi; Shigeo Kitayama The NET [noradrenaline (norepinephrine) transporter], an Na + /Cl − -dependent neurotransmitter transporter, has several isoforms produced by alternative splicing in the C-terminal region, each differing...
Articles
Articles
Articles
Biochem J (2006) 393 (2): 575–582.
Published: 23 December 2005
... residues in fragment I are differentially accessible in the SI(+) and SI(−) splice variants of the type-I IP 3 R. Alternative splicing calcium endoplasmic reticulum IP 3 receptor thiol groups Changes in cytosolic Ca 2+ regulate a wide array of cellular processes [ 1 ]. RyRs (ryanodine...
Articles
Biochem J (2005) 389 (2): 343–354.
Published: 05 July 2005
... cellular processes and several are mutated in human diseases. We report that the product of the PTPN20 gene at the chromosome locus 10q11.2 is alternatively spliced to generate 16 possible variants of the classical human non-transmembrane PTP 20 (hPTPN20). One of these variants, hPTPN20a, was expressed...
Includes: Supplementary data
Articles
Biochem J (2004) 384 (3): 647–653.
Published: 07 December 2004
... DNA probe. The sequence was 5′-CAAAAATAACAACCTGCCGCCGCGGTGCCTGC-3′. A 4 μg portion of poly(A) + RNA of K562 cells was hybridized to the probe to determine the transcription start site of the ZFF29 gene. alternative splicing fetal erythroid transcriptional activator zinc-finger protein...
Articles
Articles
Biochem J (2004) 377 (3): 641–651.
Published: 01 February 2004
... the isolation, genomic organization and in vivo expression of a mouse Dot1 homologue (mDot1). Expressed sequence tag analysis identified five mDot1 mRNAs (mDot1a–mDot1e) derived from alternative splicing. mDot1a and mDot1b encode 1540 and 1114 amino acids respectively, whereas mDot1c–mDot1e are incomplete...
Articles
Biochem J (2003) 375 (3): 689–696.
Published: 01 November 2003
... subfamily, corresponding to the classical μ- and m-calpain forms. The third, which shows peculiar activating and regulatory properties, is an alternatively spliced calpain 3 (p94) form. This new calpain differs from calpain 3 in that it has lost IS1 insertion and exon 15, a lysine-rich sequence regarded...
Articles
Articles
Articles
Biochem J (2003) 374 (1): 175–184.
Published: 15 August 2003
...′. By means of alternative splicing, the GS gene produces an altered form of GS transcript with 5′-untranslated region (UTR) containing the exon 1′. This alternative transcript is abundantly expressed in brain, whereas it is found at lower levels in other tissues. In the human and mouse GS genes, extra exons...
Articles
Biochem J (2003) 373 (1): 191–200.
Published: 01 July 2003
... isoforms of human PSAT can be produced by alternative splicing, PSATβ rather than PSATα is the physiologically functional enzyme required for the phosphorylated pathway, and indicate that the human PSAT gene is regulated depending on tissue specificity as well as cellular proliferation status...
Articles
Articles
Articles
Articles
Articles
Articles
Biochem J (2002) 367 (3): 687–695.
Published: 01 November 2002
..., we cloned and characterized two novel isoforms of Fe65L2, designated I-214 and I-245, which are produced by alternative splicing of the RNA. The splicing events disrupt the ability to bind with APP and low-density-lipoprotein-receptor-related protein. Fe65L2 was highly expressed in the brain, whereas...
Articles
Articles
Articles
Biochem J (2001) 355 (2): 279–288.
Published: 06 April 2001
... consists of four exons spanning 5.5kb on chromosome 20. Using PCR, six alternative splice variants of the H 3 receptor were cloned from human thalamus. These variants were found to be coexpressed in human brain, but their relative distribution varied in a region-specific manner. These isoforms displayed...
Articles
Biochem J (2001) 355 (2): 529–535.
Published: 06 April 2001
... numbers AF162683 and AF162684. 21 6 2000 16 1 2001 8 2 2001 The Biochemical Society, London © 2001 2001 alternative splicing Alu sequences erythroid promoter gene evolution Biochem. J. (2001) 355, 529 535 (Printed in Great Britain) 529 Transcriptional and translational...
Articles
Biochem J (2000) 351 (1): 123–132.
Published: 26 September 2000
... is a ubiquitously expressed protein with characteristic properties of a cAMP- dependent protein kinase catalytic subunit. Keywords: alternative splicing, cAMP-dependent protein kinase, isoform-selective antibody, morphological change, tissue dis- tribution. serves an in ŠiŠo function distinct from Ca comes from...
Articles
Biochem J (2000) 350 (3): 855–863.
Published: 08 September 2000
... correspondence should be addressed (e-mail [email protected] ). 29 2 2000 9 6 2000 13 7 2000 The Biochemical Society, London © 2000 2000 alternative splicing ATP7A mRNA copper transport Menkes disease gene nuclear-localization sequence Biochem. J. (2000) 350, 855 863 (Printed...
Articles
Biochem J (2000) 349 (1): 289–297.
Published: 26 June 2000
...Li ZHANG; Timothy LEE; Yue WANG; Tuck W. SOONG Natural resistance-associated macrophage protein 2 (Nramp2) has been suggested to be involved in transferrin-independent iron uptake. Two isoforms of the Nramp2 gene generated by alternative splicing of the 3ʹ exons were identified in mouse, rat...
Articles
Articles