... mutants, all expression and purification methods were the same as MurI Mtb , except that the E. coli host was BL21(DE3). analytical ultracentrifugation enzyme kinetics glutamate racemase monomer engineering Mycobacterium tuberculosis thermofluor Mycobacterium tuberculosis...

... significance of these metal sites was investigated. Analytical ultracentrifugation (AUC) and CD showed that zinc, but not other divalent metals, causes ZAG to oligomerize in solution. Thus ZAG dimers and trimers were observed in the presence of 1 and 2 mM zinc. Molecular modelling of X-ray scattering curves...

... studied. Analytical ultracentrifugation showed that all four IgA1 samples were monomeric with similar sedimentation coefficients, s 0 20,w . X-ray scattering showed that the radius of gyration ( R g ) slightly increased with IgA1 concentration, indicating self-association, although their distance...

...-mediated proteolytic inactivation of C3b into iC3b [ 5 , 6 ]; (ii) accelerating the spontaneous decay of the C3 convertase of the alternative pathway [ 7 ]; and (iii) blocking Factor B from binding to C3b to form the C3 convertase (C3bBb) [ 7 ]. analytical ultracentrifugation Factor H heparan...

... in solution which are often difficult to quantify. In the present manuscript we show that the combination of MS under nondenaturing conditions and AUC-SV (analytical ultracentrifugation sedimentation velocity) unambiguously clarifies a distribution of states and hydrodynamic shapes of assembled oligomers...

... the cluster are in a more extended conformation compared with iron–sulfur-bound human GLRX2, and the intersubunit interactions are more extensive and involve conserved residues among monothiol GLRXs. Gel-filtration chromatography and analytical ultracentrifugation support a tetrameric organization of holo...

... a single peak at 0.3 mg/ml, which resolved into two peaks that shifted to smaller r values at 1.3 mg/ml, even though the maximum dimension of IgG4 was unchanged at 17 nm. This indicated a small concentration dependence of the IgG4 solution structure. By analytical ultracentrifugation, no concentration...

...-operativity. It also increased K 0.5,malate and decreased k cat values, indicating that the catalytic power of these mutant enzymes was significantly impaired following mutation of Lys 57 . Analytical ultracentrifugation analysis demonstrates that the K57A, K57S and K57C mutant enzymes dissociate...

... chains instead of one. We analysed structural and functional characteristics of recombinant baculovirus-derived SP-A1, SP-A2 and co-expressed SP-A1/SP-A2 using a wide array of experimental approaches such as analytical ultracentrifugation, DSC (differential scanning calorimetry) and fluorescence. We...

... (email [email protected] or [email protected] ). 1 8 2006 22 8 2006 24 8 2006 24 8 2006 The Biochemical Society, London 2006 analytical ultracentrifugation CD spectroscopy ferric uptake regulator (Fur) potentiometric titration Pseudomonas aeruginosa...

... an optimized purification protocol and different detergents. Furthermore, the ATPase activity of BmrA and the quantity of bound lipids and detergent were determined, and the oligomeric state was analysed using SEC (size-exclusion chromatography) and analytical ultracentrifugation. The activity...

...John D. DORAN; Xun LIU; Paul TASLIMI; Ahmad SAADAT; Ted FOX The effect of the length of ROCK (Rho-associated kinase) on its oligomerization state has been investigated by analysing full-length protein and four truncated constructs using light-scattering and analytical ultracentrifugation methods...

... analogues (namely adenosine 5′-[γ-thio]triphosphate) to requiring hydrolysable ATP for any interaction to occur. We have investigated this further using the molecular hydrodynamic methods (hydrodynamic bead modelling, sedimentation-velocity analytical ultracentrifugation and dynamic light-scattering...

... that none of the tryptophan residues were essential for activity. The equilibrium binding of 7AWσ N to core RNAP was examined by analytical ultracentrifugation. In sedimentation equilibrium experiments, absorbance data at 315nm (which reports selectively on the distribution of free and bound 7AWσ N...